circRNA, product and application thereof

文档序号:3406 发布日期:2021-09-17 浏览:25次 中文

1. A circRNA, characterized in that the sequence has more than 90% sequence homology with SEQ ID NO. 1.

2. A product comprising a reagent for detecting the circRNA of claim 1 in a sample.

3. The product of claim 2, wherein the product comprises a chip, a kit, or a nucleic acid membrane strip.

4. The product according to claim 2, wherein the reagents comprise reagents for detecting the expression level of the circRNA in the sample using sequencing, nucleic acid hybridization or nucleic acid amplification techniques; preferably, the expression level of the circRNA in the sample is detected by adopting a high-throughput sequencing technology, a probe hybridization technology, a gene chip technology or a fluorescent quantitative PCR technology.

5. The product according to claim 2, wherein the reagent comprises a probe specifically recognizing circRNA, or a primer specifically amplifying circRNA; more preferably, the primer sequences for specifically amplifying the circRNA are SEQ ID NO.2 and SEQ ID NO. 3.

6. The product according to claim 2, wherein the sample is adipose tissue, preferably subcutaneous adipose tissue.

7. Use of the circRNA of claim 1, comprising:

use of the circRNA of claim 1 for predicting or assisting mutton quality;

use of circRNA in claim 1 for the preparation of an agent for predicting or assisting mutton quality;

use of circRNA according to claim 1 for the selective breeding of sheep with different muscle qualities.

8. Use of the product of any of claims 2-6, comprising:

use of the product of any one of claims 2 to 6 for predicting or assisting mutton quality;

use of a product according to any one of claims 2 to 6 in the manufacture of an agent for predicting or assisting mutton quality;

use of the product of any one of claims 2 to 6 for the selection of sheep having different muscle qualities.

9. A method for detecting fat content of sheep, comprising: (1) selecting sheep adipose tissues; (2) detecting the expression level of the circRNA of claim 1 in the sample.

10. The method of claim 9, wherein the fat is subcutaneous fat.

Background

circRNA is the first closed circular RNA molecule found in plant viruses and influenza viruses, generated by specific cleavage. As circRNA does not have a 5 'cap and 3' poly A structure, it is more stable than linear RNA because ribonuclease cannot degrade circRNA. Endogenous competitive circRNA can also affect miRNA activity, thereby regulating gene expression, presumably circRNA may regulate adipogenic differentiation via the above principles. The research finds that 31 circRNA molecules in a precursor adipocyte in-vitro differentiation system test can be obviously increased along with the fat differentiation process, and correlation analysis shows that the expression of the circRNA molecules can be related to the corresponding linear RNA expression state. Research on the subcutaneous adipose tissues of Laiwu pigs and white pigs revealed that circRNA _26852 and circRNA _11897 can target genes that are enriched in pathways related to lipid metabolism and disease. CircACC1 promotes fatty acid beta oxidation by promoting LO2 hepatocyte AMPK holoenzyme assembly, activity and stability. Therefore, it is speculated that circRNA is possibly involved in the regulation of lipid metabolism and can provide a potential target for lipid metabolism related diseases.

The research related to the subcutaneous fat of sheep is less at present, and in order to further discover a novel circRNA related to the subcutaneous fat of sheep, the inventor selects representative pluronic sheep (D) and small tailed Han sheep (X) with larger difference in fat deposition as research objects on the basis of a high-throughput sequencing technology, wherein the former belongs to meat and fat dual-purpose sheep, the fat hip is larger, the latter belongs to short and thin tailed sheep, the subcutaneous fat is less, and key candidate genes related to fat metabolism and fat formation differentiation are screened and identified, so that a molecular mechanism related to the fat deposition is explored.

Disclosure of Invention

The primary object of the invention is to provide a circRNA related to sheep fat, which is obtained by complete transcriptomics sequencing screening and molecular experiment verification.

A circRNA, the sequence of which has more than 90 percent of sequence homology with SEQ ID NO. 1.

Preferably, the circRNA sequence has more than 95% sequence homology with SEQ ID NO. 1; more preferably, the long non-coding RNA sequence is SEQ ID NO. 1.

It is a second object of the present invention to provide a product comprising a reagent for detecting the above-mentioned circRNA in a sample.

Preferably, the product comprises a chip, a kit or a nucleic acid membrane strip.

Preferably, the reagents comprise reagents for detecting the expression level of the circRNA in the sample by sequencing technology, nucleic acid hybridization technology or nucleic acid amplification technology.

Preferably, the expression level of the circRNA in the sample is detected by adopting a high-throughput sequencing technology, a probe hybridization technology, a gene chip technology or a fluorescent quantitative PCR technology.

Preferably, the reagent comprises a probe that specifically recognizes circRNA, or a primer that specifically amplifies circRNA.

Preferably, the primer sequence for specifically amplifying the circRNA is SEQ ID NO.2 and SEQ ID NO. 3.

Preferably, the sample is adipose tissue, and more preferably the fat is subcutaneous fat or intramuscular fat.

The third purpose of the invention is to provide the application of the circRNA in predicting or assisting mutton quality.

The fourth purpose of the invention is to provide the application of the circRNA in preparing a reagent for predicting or assisting mutton quality.

The fifth purpose of the invention is to provide the application of the circRNA in breeding sheep with different muscle qualities.

The meat performance refers to the performance in the aspects of meat yield, carcass composition, fat content in meat, meat grade, fat color, intramuscular fat content and the like.

The sixth purpose of the invention is to provide the application of the product in predicting or assisting mutton quality.

The seventh purpose of the invention is to provide the application of the product in preparing a reagent for predicting or assisting mutton quality.

The eighth purpose of the invention is to provide the application of the product in breeding sheep with different muscle qualities.

The ninth purpose of the invention is to provide a method for detecting the fat content of sheep, which comprises the following steps: (1) selecting sheep adipose tissues; (2) detecting the expression level of the circRNA in the sample.

Preferably, the fat is subcutaneous fat or intramuscular fat

"Polynucleotide" or "nucleic acid" are used interchangeably herein to refer to a polymer of nucleotides of any length, including DNA and RNA. The nucleotide may be a deoxyribonucleotide, a ribonucleotide, a modified nucleotide or base, and/or an analog thereof, or any substrate that can be incorporated into a polymer by DNA or RNA polymerase, or by a synthetic reaction. Thus, for example, a polynucleotide as defined herein includes, but is not limited to, single-and double-stranded DNA, DNA comprising single-and double-stranded regions, single-and double-stranded RNA, and RNA comprising single-and double-stranded regions, hybrid molecules comprising DNA and RNA, which may be single-stranded or, more typically, double-stranded or comprise single-and double-stranded regions. In addition, the term "polynucleotide" as used herein refers to a triple-stranded region comprising RNA or DNA or both RNA and DNA. The chains in such regions may be from the same molecule or from different molecules. The region may comprise the entire population of one or more molecules, but more typically is a region comprising only some molecules. One of the molecules of the triple-helical region is often an oligonucleotide. The term "polynucleotide" specifically includes cDNA.

Polynucleotides may comprise modified nucleotides, such as methylated nucleotides and analogs thereof. Modification of the nucleotide structure may be performed before or after assembly of the polymer. The nucleotide sequence may be interrupted by non-nucleotide components. The polynucleotide may be further modified after synthesis, such as by conjugation with a label. Other types of modifications include, for example, "caps", substitution of one or more of the naturally occurring nucleotides with an analog, internucleotide modifications such as, for example, those having uncharged linkages (e.g., methylphosphonates, phosphotriesters, phosphoramidates, carbamates, etc.) and those having charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.), those containing pendant moieties (e.g., proteins (e.g., nucleases, toxins, antibodies, signal peptides, poly-L-lysine, etc.), those having intercalators (e.g., acridine, psoralen, etc.), those containing chelators (e.g., metals, radioactive metals, boron, oxidative metals, etc.), those containing alkylators, those having modified linkages (e.g., alpha anomeric nucleic acids), and unmodified forms of the polynucleotide. In addition, any hydroxyl group typically present in sugars can be replaced with, for example, a phosphonate group, a phosphate group, protected with a standard protecting group, or activated to make additional linkages to additional nucleotides, or can be conjugated to a solid or semi-solid support. The 5 'and 3' terminal OH groups may be phosphorylated or substituted with amines or organic capping group modules of 1-20 carbon atoms. Other hydroxyl groups can also be derivatized to standard protecting groups. Polynucleotides may also contain analog forms of ribose or deoxyribose sugars commonly known in the art, including, for example, 2 '-O-methyl-, 2' -O-allyl-, 2 '-fluoro-or 2' -azido-ribose, carbocyclic sugar analogs, alpha-anomeric sugars, epimeric sugars such as arabinose, xylose or lyxose, pyranose, furanose, sedoheptulose, acyclic analogs, and abasic nucleoside analogs such as methylribonucleosides. One or more phosphodiester linkages may be replaced with alternative linking groups. Not all linkages in a polynucleotide need be identical. A polynucleotide may contain one or more different types of modifications described herein and/or multiple modifications of the same type. The foregoing description applies to all polynucleotides mentioned herein, including RNA and DNA.

As used herein, "oligonucleotide" generally refers to a short, single-stranded polynucleotide that is less than about 250 nucleotides in length, although this is not required. The oligonucleotide may be synthetic. The terms "oligonucleotide" and "polynucleotide" are not mutually exclusive. The above description for polynucleotides is equally and fully applicable to oligonucleotides.

"primer" as used herein refers to a single-stranded polynucleotide capable of hybridizing to a nucleic acid and allowing polymerization of the complementary nucleic acid, typically by providing a free 3' -OH group.

The present invention may utilize any method known in the art for determining gene expression. It will be appreciated by those skilled in the art that the means by which gene expression is determined is not an important aspect of the present invention. These techniques include, but are not limited to: nucleic acid sequencing, nucleic acid hybridization, and nucleic acid amplification techniques.

Illustrative, non-limiting examples of nucleic acid sequencing techniques include, but are not limited to, chain terminator (Sanger) sequencing and dye terminator sequencing. One of ordinary skill in the art will recognize that RNA is typically reverse transcribed into DNA prior to sequencing because it is less stable in cells and more susceptible to nuclease attack in experiments.

Another illustrative, non-limiting example of a nucleic acid sequencing technique includes next generation sequencing (deep sequencing/high throughput sequencing), which is a unimolecular cluster-based sequencing-by-synthesis technique based on proprietary reversible termination chemical reaction principles. Random fragments of genome DNA are attached to an optically transparent glass surface during sequencing, hundreds of millions of clusters are formed on the glass surface after the DNA fragments are extended and subjected to bridge amplification, each cluster is a monomolecular cluster with thousands of identical templates, and then four kinds of special deoxyribonucleotides with fluorescent groups are utilized to sequence the template DNA to be detected by a reversible edge-to-edge synthesis sequencing technology.

Illustrative, non-limiting examples of nucleic acid hybridization techniques include, but are not limited to, In Situ Hybridization (ISH), microarrays, and Southern or Northern blots. In Situ Hybridization (ISH) is a hybridization of specific DNA or RNA sequences in a tissue section or section using a labeled complementary DNA or RNA strand as a probe (in situ) or in the entire tissue if the tissue is small enough (whole tissue embedded ISH). DNA ISH can be used to determine the structure of chromosomes. RNA ISH is used to measure and locate mRNA and other transcripts (e.g., ncRNA) within tissue sections or whole tissue embedding. Sample cells and tissues are typically treated to fix the target transcript in situ and to increase probe access. The probe is hybridized to the target sequence at high temperature, and then excess probe is washed away. The localization and quantification of base-labeled probes in tissues labeled with radiation, fluorescence or antigens is performed using autoradiography, fluorescence microscopy or immunohistochemistry, respectively. ISH can also use two or more probes labeled with radioactive or other non-radioactive labels to detect two or more transcripts simultaneously.

The present invention can amplify nucleic acids (e.g., ncRNA) prior to or simultaneously with detection. Illustrative non-limiting examples of nucleic acid amplification techniques include, but are not limited to: polymerase Chain Reaction (PCR), reverse transcription polymerase chain reaction (RT-PCR), Transcription Mediated Amplification (TMA), Ligase Chain Reaction (LCR), Strand Displacement Amplification (SDA), and Nucleic Acid Sequence Based Amplification (NASBA). One of ordinary skill in the art will recognize that certain amplification techniques (e.g., PCR) require reverse transcription of RNA into DNA prior to amplification (e.g., RT-PCR), while other amplification techniques directly amplify RNA (e.g., TMA and NASBA).

The polymerase chain reaction, commonly referred to as PCR, uses multiple cycles of denaturation, annealing of primer pairs to opposite strands, and primer extension to exponentially increase the copy number of a target nucleic acid sequence; transcription-mediated amplification of TMA (autocatalytically synthesizing multiple copies of a target nucleic acid sequence under conditions of substantially constant temperature, ionic strength and pH, wherein multiple RNA copies of the target sequence autocatalytically generate additional copies; ligase chain reaction of LCR uses two sets of complementary DNA oligonucleotides that hybridize to adjacent regions of the target nucleic acid; other amplification methods include, for example, nucleic acid sequence-based amplification commonly known as NASBA; amplification of the probe molecule itself using RNA replicase (commonly known as Q.beta.replicase), transcription-based amplification methods, and self-sustained sequence amplification.

In the present invention, the product comprises a reagent for detecting CIRCRNA, and one or more substances selected from the group consisting of: container, instructions for use, positive control, negative control, buffer, adjuvant or solvent.

The kit of the invention can be accompanied with instructions for using the kit, wherein the instructions describe how to use the kit for detection, how to judge the tumor development by using the detection result and how to select a treatment scheme

The invention has the advantages and beneficial effects that: (1) the sequence shown as SEQ ID NO: 1 and the circRNA related to the performance of the sheep meat; (2) in actual production, the meat performance of sheep can be improved and the meat feeling can be improved by regulating and controlling the expression of circRNA, so that the invention provides theoretical basis and technical revelation for animal husbandry production.

Drawings

Figure 1 is an adipose tissue differential expression circRNA GO annotation;

FIG. 2 is an adipose tissue differential expression circRNA KEGG pathway enrichment assay;

FIG. 3 shows the results of the validation of the circRNA gene differential expression qRT-PCR.

Detailed Description

The present invention is further illustrated below with reference to specific examples, which are intended to be illustrative only and are not to be construed as limiting the invention. Those of ordinary skill in the art will understand that: various changes, modifications, substitutions and alterations can be made to the embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the claims and their equivalents. The following examples are examples of experimental methods not indicating specific conditions, and the detection is usually carried out according to conventional conditions or according to the conditions recommended by the manufacturers.

EXAMPLE 1 Collection agent RNA extraction of samples

1.1 Collection of samples

The test was conducted on Doudang sheep (D) and small tailed han sheep (X) with differences in fat deposition. The selected sheep were all adult females, and the individuals were well-conditioned, healthy and disease-free, of similar body weight (about 50kg), with three biological replicates per group.

1.2 extraction and quality detection of sample RNA

Equal amounts of adipose tissue were removed from each sample for total RNA extraction, ensuring that the entire procedure was performed in a sterile environment, as follows:

(1) respectively grinding adipose tissues in a mortar added with liquid nitrogen;

(2) placing the ground tissue into a centrifuge tube added with Trizol reagent for standing;

(3) adding chloroform, mixing and standing for 10 min at room temperature;

(4) placing the mixture into a centrifuge, and centrifuging the mixture for 15 minutes at the rotation speed of 12000 under the condition of 4 ℃;

(5) putting the colorless liquid at the uppermost layer into an RNase free tube, precipitating RNA by using isopropanol with the same amount, uniformly mixing at room temperature and standing for 10 minutes;

(6) centrifuging again for 15 minutes at the temperature of 4 ℃ and the rotating speed of 12000;

(7) removing supernatant, adding 75% ethanol into the precipitate, mixing, and centrifuging under the above conditions;

(8) removing supernatant, and continuously adding ethanol to wash RNA2-3 times;

(9) removing ethanol, and drying the precipitate for 5-10 min;

(10) finally, the precipitate is treated with RNA enzyme-free water.

To ensure the purity and integrity of total RNA, the total RNA was subjected to quality testing:

the requirement of library construction and sequencing is that the Total amount of RNA is not less than 2ug, a TruSeqTM Stranded Total RNA Kit is adopted for the library construction of longRNA (mRNA, lncRNA and circRNA), the Total amount of RNA is 2 mug, the concentration is more than 100 ng/muL, and the OD260/280 range is 1.8-2.2.

Total RNA is extracted from 6 subcutaneous adipose tissues of the Wulang sheep and the small-tailed han sheep respectively and quality detection is carried out, and the result shows that the brightness of a 28S strip is obviously higher than that of 18S strip; the RNA concentration of each sample is higher than 200 ng/mu l; RIN values are all larger than 8, OD260/280 is larger than or equal to 1.8, OD260/230 is larger than or equal to 1.0, and the result shows that RNA is not polluted by carbohydrate, protein and other impurities, the integrity is good, and the RNA can be continuously applied in subsequent tests.

Example 2 sequencing and data analysis

Sequencing was performed using the Illumina NovaSeq 6000 sequencing platform. The circRNA is in a closed ring shape, is formed by reversely connecting exon splicing acceptors and donor sites which are respectively positioned at the upstream and the downstream, belongs to a non-coding RNA molecule, has no influence on the non-coding RNA molecule by RNA exonuclease, is not easy to degrade, and is more stable. Linear RNA fragments can be aligned by linear alignment software, but Reads in trans-splicing positions of circular RNA cannot be aligned directly to the genome. circRNA prediction was performed using correlation software (CIRI2+ find _ circ collection) based on Back spot connections (BSJ) reads and compared to the database circBase (animals).

Classification is based on the starting and ending points of circRNA on the genome. The first type of termination point and the initiation point are both located in the exonic region of the gene and are exon circRNA. One end of the second type of initiation or termination point is located in the intron region of the gene and is intron circRNA. A third class of circRNAs is classified as endogenous circRNAs when either the start or stop point is located in the intergenic region or when both start and stop points are located in the exon regions of a gene but the two exons are not in the same gene.

And analyzing the difference of sample expression. Using rpm (reads per millon reads) as a quantitative index, the calculation formula is:

RPM=Total exon reads/Mapped reads(Millions)

wherein, Total exon reads represent all reads from a certain sample Mapping to a specific gene exon; mapped reads represent the total amount of all reads for a sample. 18947 circRNAs are obtained in subcutaneous adipose tissues of Dolang sheep and small tailed Han sheep, differential expression analysis is carried out on the data, (screening is carried out by taking the standard of | Fold Change | ≧ 2.0 and Pvalue < 0.05), 141 circRNAs with differential expression coexist in subcutaneous adipose tissues of two varieties of sheep, wherein 61 differential genes are up-regulated and 80 are down-regulated. The circRNA-NC-040256.1-12467238-12540333 with remarkable differential expression is found for the first time in the application as a candidate molecule for the next time.

To understand the function of differentially expressed genes and gene products, three-way annotation analysis was performed on differentially expressed genes using GO annotation, including biological processes, cellular components, and molecular functions. The source genes of the different circRNAs are analyzed, and the enrichment is found to be 379 entries in total, and 99 entries are screened under the condition that Pvalue is less than 0.01. As shown in fig. 1, the differentially expressed circRNA-derived gene is mainly enriched in terms of translation inhibitor activity (translational activity), leptin receptor binding (leptin receptor binding), triphosphatase activity (triphosphatase activity) and the like in terms of molecular functions; in the cellular fraction, it is mainly enriched in ribonucleoprotein granules (ribonucleoprotein granules), fibrillar collagen trimers (fibrillar collagen trimers), nucleotide-activated protein kinase complexes (nucleotid-activated protein kinase complex), cytoplasmic ribonucleoprotein granules (cytoplasmic ribonucleoprotein granules); in biological processes, protein activation cascade (protein activation cascade), plasma membrane bound cell projection assembly (plasma membrane bound cell projection assembly), vesicle cytoskeletal transport (vesicle cytoskeletal transfer), and regulation of TOR signaling (regulation of TOR signaling) are among the processes. Through GO function research, the source gene of the differential expression circRNA is found to be involved in fat development by regulating and controlling leptin receptor binding enzyme, triphosphatase and other enzyme activities related to fat deposition.

In subcutaneous adipose tissues of a pluronic sheep and a small-tailed han sheep, the source genes of the circRNA are enriched into 110 passages (fig. 2, screening is carried out under the condition that Pvalue is less than 0.05, 3 remarkable enrichment passages are obtained in total), Protein digestion and absorption (Protein differentiation and absorption), Glucagon signaling (Glucagon signaling pathway) and lipolysis (Regulation of lipolysis in adipocytes) are obtained, KEGG passage analysis shows that the source genes of the circRNA are differentially expressed in the subcutaneous adipose tissues of the pluronic sheep and the small-tailed han sheep and participate in the passages related to fat metabolism, and the differential genes enriched in the passages can play an important role in regulating and controlling the subcutaneous fat deposition of the two varieties.

Example 3 real-time fluorescent quantitative PCR assay validation

In the experiment, 20 pluronic sheep and small-tailed han sheep are respectively collected, PCR detection is carried out on the circRNA with differential expression between the two groups, each gene is repeated for 3 times, and the differential expression condition of the NC-040256.1-12467238-12540333 gene is verified.

The main method comprises the following steps:

reverse transcription was performed using the circRNA cDNA Synthesis Kit (GenePool, Cat # GPQ1805) in the following reaction scheme: 2 × FastSYBR texture, 10 μ l; 0.4. mu.l each of the downstream primer (10uM) and the upstream primer (10 uM); template, 2. mu.l; distilled water was sterilized to 20. mu.l. The primer information is shown in the table.

TABLE 1 PCR reaction results

After the reaction is finished, data are derived, using 2-ΔΔCtThe relative expression of the samples is calculated by the method, the relative expression is analyzed by t-test statistics, the data is shown as Mean +/-standard deviation (Mean +/-SD), and the significance is shown when P is less than 0.05. Analysis of PCR data and results, using 2-△△CtThe method calculates the relative expression of CIRCRNA gene among samples, and data are expressed as Mean + -standard deviation (Mean + -SD), P<0.05 indicated that the gene differed significantly between the two groups. The results show that the NC-040256.1-12467238-12540333 gene is significantly up-regulated in subcutaneous adipose tissues of small tailed han sheep.

The method obtains the circRNA gene closely related to fat deposition through functional annotation and enrichment analysis screening, and the conclusion is further verified through a fluorescent quantitative PCR experiment result.

Sequence listing

<110> Beijing animal husbandry and veterinary institute of Chinese academy of agricultural sciences

<120> circRNA, product and application thereof

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<170> SIPOSequenceListing 1.0

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<213> Artificial Sequence (Artificial Sequence)

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catcattcca gtacactctt agggaacgga catatatgtg ctgtaccttc tgtgttccaa 180

gatgggtgat gccaatctga acacaatgga aactagtaga cactcgtatc taaggaatga 240

gttttactcc agtatccttt ctgtaacaat gtgctttgag aactaacagc attatcatga 300

ctcttgtttg aaatacgcat tctcaagagt tcacctgtgg cacagtgaat gaaactttga 360

aatttgggcc tagttatttc tgttttaaca agcaatggac attattctct tggacactgt 420

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gtctgtcaac aaattttgat tatgttcaat ttggggatat cctaacctga ggttcaaagt 1920

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ggacatctga aaactcacac tggagagaaa ctctatgaat ataaggaatg tgggaagctt 2760

ttgggtcatt gtcatacctt catgttcaca gtagaattca tgctggaata aaaccttata 2820

aatgtcaaga atgtgggaaa acctttactt tcccctcttg ccttattgaa catatgaaaa 2880

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gtaatcttct tagacattta cgatctcata cagggcagaa gactgctaaa tgtgccagat 3000

gtgggaaagc atttgcttga tcctcacaag ttactcaaca tatgagaaca cacactggag 3060

agaagccttg taagtgtaac acatgtggga aagcctttca gttttccagt aatcttctta 3120

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tgctcctcaa tgcttacttg ctatatacaa tgacattgga aaagaagtca ctgaaatgtg 4080

acaaatgtgg taaagccctt gttgattcct cctatcatac ttagcatttt atttttaata 4140

tttgactgca tctgcagcaa atgagacctc agttctctga caaaggataa aacccatccc 4200

cctgcattgg aatgcagagt cttgacctct gaacaaccag agaagtccca tactaaacat 4260

ttttgaactc aacataaaag agaatccttt tgagtgtaat aaatttcagt aagactcgcc 4320

aattcttcac cttttcaaat aatgaatgaa ataccaatta gcgttaatct gggattgcct 4380

catgaccatt ttttgctgac acagtgaaac cttgaccagg ttcgacgcag aaactgaaat 4440

tcatctattt gtacttctga tgtggcacag aagggcagag gattaggcac agctctactt 4500

tgtacaacat tgaataaatt accccactac ctcacaagaa agagtaaaga caatttaata 4560

tttatacatc cagtattttt cctctgtcct gtaactcttc tatcgacatc ctaagaccac 4620

ttgtgaaaga gagggaaaaa atatggtttg ctttaaaatc ataaaaatat ggtatcttca 4680

tatgataatc ttgatacaga aaaagccttt gacaaaatcc agcacccatt tatgattaaa 4740

aactcttcaa aaaaatggtc atagaaagaa cctacctcaa tatagaaaag accatacaaa 4800

tacacctaca gcaaacatta ttctcatggt gaaaagctga aagcattccc tctaagatca 4860

ggacaagaca agggtgtcca ctttcaccac tattattcaa catagttctg gaagtcctag 4920

ctacagcaaa cagagaagaa aaaaaaataa aaaaatccag attggaaaag aagaagtaaa 4980

aatctcactg tttgcaggtg acatgataca gtacacagaa aaccctaaag atagtattag 5040

aaaattacta ggactaacca gtgaatttag caaagttgca agatacaaaa tcaatacaca 5100

gaaatcactt gcattttgta ctaacaatga aaaatcagag aaattaagga atcaatccca 5160

ttcaccactg aaacaaaaag aattaaatat ctaggaataa gcttacctaa ggagacaaaa 5220

gaactgtaca cagaaaatta taagacactg atgaaagaaa tcaaagatga cataacagat 5280

ggagatattc catgtttctg gataggaaga atcaatgttt gtgaaaatga ctatactacc 5340

aaatggaatc tacagattca atgtgatccc tatcaaacac catgactttt tcacagaact 5400

agaacaaaaa aagtcacaat tcgtatgaaa aaacaaagac cctgaatagc aaaacagtct 5460

tgagaaagaa gaatggagct agagaaatca accttcctga cttcagatta tactacaaag 5520

ctacagtcat caagacggta ctggcacaaa aacagaagta tagaccaatg gaacaagata 5580

gaaatcccag aaataaaccc atgcacctat gggtacctta ttttttgaca aaggaggcaa 5640

gaatatacaa tggggcaaag atagcctctt caataaatgc tgctgggaaa actggaccac 5700

tacatgtaaa agagtgaaat tagaacactt tctaacacca tacacaaaga taaactcaaa 5760

atggattaaa gacctaaatg taagataagg agctataaaa ctataaagga agatctacaa 5820

aactataagg caagatcctc tgacccacct cctagagtaa tggaaataaa aacaaaaata 5880

aagtgagacc tgattaaact taaaatcttt gcacagcaaa ggaaactata gcaaggtgaa 5940

aagacaaccc tcagaatggg agaaaataat agcaaatgaa acaactgaca aaggatttcc 6000

aaaatataca agcagctaat acaactcaat gccagaaaaa caaaaaaccc aatcaaaagg 6060

tgggggaaag acctaaacag gcatttctcc aaagaagaca taaagatggc taacaaacac 6120

atgaaaagat actcaacatc actcattatt agagatgcaa atcaaaatta taatgagata 6180

tcacctcaca ccactcagaa tgaccatcat caaaatgtct acaaacaata aattctggag 6240

agggtgtgcg aaaagggaat gctcttgccc tattggtggg aatctaaatt gatacagcac 6300

tatggaagat tgtatggaga ttccttaaaa aactaggaat aaagctacca atatgaccca 6360

gaaatcccac tcctaggcac ataccctgag gaaaccgaaa ctgaaaaaga cacatgtatc 6420

ccattgttca ttgcactatt tacaatagct acaacatgga agcaatctag atgttcatca 6480

atggacgaat ggatataaaa gttgtggtac atatacacaa tggacgtaaa aggagcacat 6540

ttgagtctta aaaggagcca taaaaggagc acatttgagt cagttttaat gaggtggatg 6600

aacctagaat ctattataca ggtgaagtga gtcagaaaga ggaagataaa tatcatattc 6660

taatggatat atatggaatc tagaaaaatg gtactgaata agttattttc agggcagcaa 6720

tggagaagca gacatagaga gtagatttat ggacatggag agacgggagg agaggtgata 6780

tgtatggaga gagtaacatg aaaacttaca ttaccatatg taaaatagat agccaatagg 6840

aattttttgt atgtctcagg aaacccaaac aggggctttg tatcaaccta gaggtatggg 6900

atggggaggg agatgggagg aggttcaaaa gagagatgat atatatatac ctatggctga 6960

ttcatgttga ggtttgacag aaaaccacaa aatttctgta aagcaaatta tttcttcaat 7020

aaaaaattaa attaattttt aaaaatcatg gtttgagttt attatcagga cattcaatag 7080

gatctatttt tttctctaag gctacgtgcc agactgagac tcaaattaga acaaagtgtg 7140

ggatttgaat atttctcaca gaataaaggg atacaaaagt gaggacagaa atttctcagt 7200

gatgcatcaa atactgaatg tgagaagatt ctgttatctt tacagatact catttatgta 7260

aagaaaactg agtcaagcac aaagcaaatc gctaatttac tacatagaga cctaatatct 7320

atcttttact atacaccgtg tcttctacac tcatccttag cactgactct aatgcctgat 7380

accttctccc cacatgctat gacttcagtt taacatttta ccattgccca catattttaa 7440

agaggcccaa tactctctcc catctctcta taattaaccc tactcagttg ccatttgatc 7500

aagaatactc agggcttcct gggtagctca gctggtaaag aatctgcctg caatgcagta 7560

gactccagtt ctatacctag gttgggaaga tcccctggag aaaggataag ctacccactc 7620

catattcttg ggctttctgg tggctcagat ggtaaaagaa tctgcctgca atgcaggagg 7680

acctgggttc gacccctggg ttgggaagat cgcctggagg agggcatggc aaccccactc 7740

cagtgttctt gcctggagaa tccacatgta caggagagcc tggtgggcta caatacaaca 7800

gggtcacaaa gagtctgact tgactgagtg actaagcaca gtttagcaac caggatactc 7860

atcataaaag agggaattgg gaaaagaagc agtacatgtc acatttatgg tataagcata 7920

atgagccttg gttttccatg catcatgttc tttttttttc tttttttctc caatggtttc 7980

tttttttaat ttttgttttt gctttatttt actttacaat actgtattgg ttttgccata 8040

cattgacatg aatccgccac gggtgtacat gagttcccaa tcctgaaacc cccctcccac 8100

cttcccaccc catatcatct ctctggatca tccccatgca ccagccccaa gaatcctgaa 8160

atcctgaatc gaacatagac tggtgattca tttcttacat gatagtatac atgtttcagt 8220

acaattctcc caaatcatcc caccctctcc ctcttcctca gagtccaaaa gtcccgttct 8280

atacatcttg tgtctctttt gctgtctcac atgcctcaat gttctataga ttcatctttg 8340

tcattatcct agaatcttat ctcatctact caactatgaa aataacttca gtttaagtaa 8400

tacatttttg ggcttcctgg gtggtgctag tggtaaagaa cctggctgct aatgcaggag 8460

acataagaga cgcgggttca atccctgagt ctggaagatc ccctgaagaa gggccatgga 8520

aacccactcc ggttactctt gctcggggaa tcccatggac agaggagcct ggcgggatac 8580

agcccaaaaa ggtcaataag agtcgaacac gactgaagtg acttagcaca catgcacagt 8640

tatacatttt gctgtggaat gaattatttt tgatgcccaa aacccagtgt cttcctctca 8700

tttaggatga ggttttctgt gtggtgggca gtatctgaga ttctctcact gatccaaact 8760

cctggttttc ctacccctgt gtatttccct gccattgagt gcaagatgta cataaagacc 8820

cacttccaac aacaagaatg tgaaagaaat gtcgggaagt cattcttagc tggaacagag 8880

caaaataatc tggctctctt cttggtgttt tctctctggc attcttggtg ccttgcatta 8940

ataaaatcca actgcctcac caggaggtca catgtcaagg aactgaagga attctctgga 9000

caatattcca tgaagaagtg aatcctacca acagcctcat gagtgagctt gagagtggat 9060

atctgctgat ctaactttgt gatacctgca gcctgactga catcttgatt gcaacattct 9120

gggagatgca ctgaaatatt taaagtatca gtgagtaaat tataagtaaa gatcaatagt 9180

tttctaatat gccaatgtca atagagaaaa aaacttaaaa ctgaagactc atggtttggc 9240

agtggtttag ttgctaaatc atgtctgatt cttgtgaccc caagtgaaga ttcattaaaa 9300

ttagccaaaa aaaaaagaga gaagctatcc tttgagacaa atactaactg tgaaaatgga 9360

aagtatatca cattcttggt agagacctaa tactacatag atttcatgat tgatactact 9420

gatttgtaga tatcaaaata ataccattga gtggctaaat tggaggaggg agggtctgaa 9480

ttttatgtta agtacctgca agttagaagc aataagtaaa agtacaggtt aatttcctcc 9540

tgaggagaag aggggcccag atcattccta ggacattcat ttttctcctt tcctgttctg 9600

ttaatattat caagtctcca gagattggga taatgtggac cagcttgcta atgatgagtt 9660

ttggaagttg agccctaagg tcataccatt gtaaataact gggcagtgcc tctagctaat 9720

aatttgcctg ttctaatact gtgagtccac acctgtgtct cctcttcata ggaatctgca 9780

tcagaaggtc cagcctgagt ctgtctttca ctgcttcaac ctgaggagaa ggaccctgag 9840

actttatcac acaccaggca ggagagggtg agtgagtctg tgctccttgg tcatgctcca 9900

gcagagatgc agcccagccc ccgtaagtat tgagggagac agtgatcgaa ggacaaactg 9960

ttgggagcat tattccctcc acaagaggaa caaactgata ttttacttgg tagagaaatc 10020

tggagggcac cgatctgctc agttttcaaa tagagcactt aaaatctaca ttcgaggatc 10080

tgggctgggg tgtgagaact ctcattcaga gttctacttc caccaccact ggttctgact 10140

ctggctggaa atcccttccc accagaggag ctaatggact ctgtaacctt ccccctgaac 10200

acttcagttc attatgagag aagtgatgag gatgaagctg aggacaagga tgaagaatgt 10260

gacatcaacc ataacatcat gtactgttat gaccatagaa catcacagca tcatagatat 10320

ctggctctga agtgtttaca gtctggagga caagactaac aggtatataa aaaggcactc 10380

aacatcagtt aatatcagaa acacaggtcc aaaccagatc aaagatcacc tcataactgt 10440

taggatggct attaaaaaca atcaaaagaa aacaaatgct ggtgaggaag tggagagata 10500

agggaactgt tgtacattgt tggtgggaat gaatattggt accgtcgttg gagaaacaga 10560

atggatgttc tttaaaacgg tatttttcac agagctagaa caaataattt cacaatttgt 10620

atggaaatac cgaaaaactc aaatagccaa agcaatctta agaaagaaga atggaactgg 10680

aggaatcaac ctgcctgact tcaggctcta ctacaaagcc acagtcatca agacagtatg 10740

gtactggcac aaagacagaa atatacatca atggaacaaa atagaaagcc cagagataaa 10800

tccatacaca tatggacacc ttatcttcga caaaggaggc aagaatatac aatggagaaa 10860

agacaatctc tttcacaagt ggtgctggga aaactggtta accacttgta aaagaatgaa 10920

actagaatac tttctgacac catacacaaa aataaactca aaatggatta aaaatctaaa 10980

cggaagacca gaaactataa aactcctaga ggagaacata ggcaaaacac tctcagacat 11040

aaatcacagc aggatcctct atgacccacc tcccagaata ttggaaataa aagcaaaaat 11100

aaacaaatgg gacctgatta aacttataac cttctgcaca acaaggaaac tataagcaag 11160

gtgaaaagac agccttcaga atgggagaaa ataataccaa atgaagcagc tgacaaacaa 11220

ctaatctcaa aatatacaag caaatcctgc agctcaattc cagaaaaata aatgacccaa 11280

tcaaaaaatg ggccaaagaa ctaaatagac atttctccaa agaagacata cggatggcta 11340

acaaacgcat gaaaagatgc tcaacatcac tcattatcag agaaatgcaa atcaaaacca 11400

caatgaggta ccatttcatg ccagtcagaa tggctgagat ccaaaagtct atgagcaata 11460

aatgctggag agggtgtgga gaaaagggaa ccctcttaca ctgttggtgg gaatgcaaac 11520

tagtacaatt atggagaaca gtgtggatat tcctttaaaa actggaaata gaactgcctt 11580

atgacccagc aatcccactg ctgggcatac acactgagga aaccagaact gaaggagaca 11640

catgtacccc aatgttcatc gcagcactgt tgataatagc cagaacatgg aagcaaccta 11700

gatgttcatc agcagatgaa tggataagaa agctgtgata cagatacaca atggagtatt 11760

actcagccct taaaaagaat acatttgaat cagttctaat gaggtggatg aaactggagc 11820

ctattatacc aagtgaagta agccagaaag aagaacacca atacagtata ctaacacaca 11880

tatatatgga atttagaaag atggtaatga taaccctgta tgcgagacag caaaagagac 11940

acagatgtat agaacagtct tttgaactct gtgggagaag gcgaggatgg gatgatttgg 12000

gagaatggtg ttgaaacatg tataatatct attggatgaa tcgccagtcc agcttcgatg 12060

catgatactg gatgctttgg gctggtacac tgagacgacc cagagggatg gtacggggag 12120

ggaggagggc ggggggttca ggatggggaa cacgtatata cctgaggtag attcatgttg 12180

atgtatggca aaaccaatac aatattgtaa agtaattaac ctccaattaa aataaataat 12240

ttttttaaaa aaattttaac tgcaacaaat tgaaatagaa ctagaaacca gaaaccccac 12300

ttcagagtat tggccaagaa ataaaatcag accctcaaaa gtgagtattt gcattccctt 12360

gtctttgcaa tattatttga aatagtgaaa atatggatct gttgacagat aaataggtaa 12420

agaaaatgag atgtgtatat ataaacaatg ggatattatc tagccacaga aagaagaaaa 12480

tcctgccatt tttgacaaca tggatggacc tggaaaatac tgtgcttatt aaactatata 12540

taccagtcat agaaagtcaa atccactatg atctcagttc tgtgtggaag ctaagaaaat 12600

cacactcttg ttgtgaggat tggtggtaga aaaggtgagg aaatggtggt caaaatgcac 12660

aaacttttgt tacaaaatga gtaagtttgg ggatctaata tgcagcctga taactatagc 12720

taataattct ggttgtgttc ttgaaatctg ctgagagaga tctgcttaac tgttactaca 12780

cacacacaca caaacacaca catgcacgcg cgcacacaca cacacacatt cacagaagct 12840

atgtgaggta ccacatacat tatttaactt gactgtggta atcattccca atgtgtattg 12900

tattaaatca tcattttgta catcttttaa tgatctccat gcggcacaat tcaaatatat 12960

gaaattttgt caatcacttc agtaaactta agaaaataaa atcagtgaag tattattttt 13020

ctgccagtac agaatagagc ccccaaaggt aaagggtctc atccatcaaa catttaaatg 13080

agatggtatt gaaacaagta accctgttta agattaaaaa tttcaagagg atttcttttt 13140

tgttttttaa agaggatttc ttaaatttca ttattgtgtt atctttagca gtgctgtgta 13200

catgaacacc atctttatag tcaacaacac attagatcag gtacacttaa acaatattga 13260

tcgaaatttg aagtccacca aagtcaaaga tatgaaatat atatatacta aatgacttct 13320

aaaaaagctc tgatacttga ataatccctt atttcataaa ggagagaaga cagaaaaaca 13380

aaggatgata aaactctgag tatcatataa aatgagttct gattatgaac atcaccttta 13440

atttagaacc cagatgacat atctctgcat ttcatagaga tttataaata ttccaagtgg 13500

aggaaatatc tagaattgta tttgaataaa ataagattta tataaatgac ttaacttctt 13560

tatatttgca tttttacatg ctttcttaga agggaaaggg actctcattc tgtattatgt 13620

ctaaagaagt aggctgtctt taactgaggc catggatgta ttacctctaa tatccttcct 13680

ccctttcact tttcatgaag ctttataaca aatgtaagag agtatagaca gaagttaaaa 13740

acacagaaac caacaggaaa caattctaag aaaatagaat ttgtcacact gagaaatatt 13800

gctgaaggaa ctgggatgta taatctgaga aatgaattac ctgagtccaa aatatggaca 13860

cagtactgca tgaccattta tgtatttata tttttccagt cctcttttct tcttccgaag 13920

gtgtccaagc tacacagaac tggaaatcta acaagtgtct cagaattctt cctcctggga 13980

ctctcagatg atccagaact gcagcctttg ctctttatcc tcttcctgtc cgtgtacctg 14040

gtcaccatgc tggggaacct gctcatcatc ctggccacca cctctgaccc ccacccccga 14100

cccccacccc cacactccct tgtacttctt cctctccaac ctctccttgg ctgacattgg 14160

cttcagctcc accacggtcc ccaagatgat tgagaacatt caaactcaca gcagagtcat 14220

ctcctatgca ggctgcctga cacacatgtc tatttttata ctttttggat gtatggatgg 14280

tatgctgctg tctgtgatgt cctatgacag gtttgtggcc atctgtcacc cactgcacta 14340

cctggttatc atgaacccac acctctgttg ctccttagtt ttggtgtctt ttttttggta 14400

gccttatgga ctcccaggta cacaatctga ttgtgctgca acttacctgc ttcaaatttc 14460

taatttcttc tgtgaccctt ctctgctcct caagcttgcc tgttttgaca ttttcaccaa 14520

aaatatagtc atatatttta tcggtgccat ttttgctttt ctccctttct cagggatctt 14580

ttactcttac tataaaatta tctcctccat tttgagagtc ccatcatcag gtgggaggta 14640

caaagccttc tctacctgtg gctcacacct ggcagttgtt tgcttatttt atggaacagg 14700

tcttggtgcg tacctcagtt cagccatctc acaatctccc aggaaggatt cactggcctc 14760

agttatgtac actgtggtca cccccatgct gaaccccttc atctacagcc tgaggaacaa 14820

agacttcaaa agggccttgt gcaggttcct caacaaaaca atctaacttc catttggagt 14880

gtagattgga aaacgtagta aaattaacat ctagatctga aaattctacc tccctcatga 14940

catcagttgt agctcttagg gccttcttgc ctttccttgt tttacacctg aatattgctt 15000

ctttggatat gtttttgatg gggctaggag ggtcttctgg gattctgcag atgtcagaac 15060

tactgtgact gaatcttatt atacccaaga aggagtctag tttattatta ttatttatta 15120

ttgtggtgac ccacatacta gaaagatgaa caactccatt ttttacagat ttaaatagta 15180

tatttttttt tctaaagctg ttgtctattt tccagctgat tactatgttt taagtctgtg 15240

tatgcagtct acgtgtgagg atacatgtta tggcttctca gccttggctt ttaacaaaat 15300

aatctgggtg gggcgggggg cttacaaata ctgactattt tgcaacattg taattcaact 15360

atcctacaat aaaataaatt ttaaaaaata ctgtcaactg agactcactg ccaaagagtc 15420

tatttattct attcttattt atttttaaat gtattactta ttttaaagga ggaaaaatac 15480

ttcacaatgt taggttgtct tgaactgggg attttttaaa attccttata tctgttaatt 15540

tttgggcata acatgcagct tgtggggtct taattccaag accggggatt gaattcatgc 15600

ctcctgcatt caaagacaga atctaagcct ctgggccacc agggaagtcc cagcatgagg 15660

atttttaaac acactgaggc tggttctacc gtgtagccaa ggttgagaac ttctgatcta 15720

agtcagatct ttcagtcaaa tgtgaccttt atgtcatgtg tcctaactgc tcttggacaa 15780

atgtcccaga tcctaaagag atctgagctc atgggggaag atataaagag cctgctatag 15840

taacaggcaa catgcctgtg tgcatattca gtcagtaagt tgcacctgac tctctagtga 15900

tcccatggac tgtagcctgc caggctattc agtccatgat atttttcagg caagaatact 15960

ggagttgccc tttccttctc cgggggatct tcccgaccca aggatggaac ccacatctct 16020

tgaattggaa ggcatattct ttaccactgc acaacttgag aagccaaaca ggcaacatgg 16080

tttccccatt attgtcaata atgattcttc acatatagcc tctctacacc atagagtttg 16140

gtctctatgg cttatgtgct gtttctgaag ctattatctc tgctccaaac tcatgcttgt 16200

atacattgct ttggagtgtg actctggctt tggagtgtga ctctggaaat aaccgctcta 16260

ttttctattt tgtgttcatt ttacatacat tccatagtgg tcagtaaaat tctctttgcc 16320

ctaaattctc aactgttcct ttcatattcc tcaagccctt ggtggagtac aatctttctt 16380

cacccacaaa tgctatgcta ttgctttgtt cttttttttg cctgttagtc tttcagtatc 16440

ttttaaacca actccgtttc agttccattt agttcagtta ctcagtcgtg tctgactctt 16500

tgcaacccca cggactgcag cacgccagga ttccctgtcc atcaccaaca tccagagctt 16560

gctcaaactc atgtccattg agtcagtgat gccatccaac catctcatcc tctgccatcc 16620

ccttctcctc ctgccttcac tgtttcccag catcagggtc tttttcaatg agtcagttct 16680

tcacatcagg tggccaaagt attggagttt cagcttcagc atcagtcctt ccaatgaata 16740

gttaagactg atttccttta ggattgactg gtttgatatc cttgctgtcc aagggactct 16800

caagaatctt ctccaacgcc acatttcaaa ggtatcaatt ctttggtgct cagctttctt 16860

tatggtccaa ctctcacatc catacatggc tactggaaaa accatagctt ttggactata 16920

tggacctttg tcagcaaagt atgtctctgc tttttaatat gctgtctagg tttgtcacag 16980

ctcttcttcc aaagagcaag catcttttat catggctgca gtcaccatct gcagtgattt 17040

tggagcccag gaaaatagtt tatcactgtt tccattgttt ccccatctgc ttgccatgaa 17100

gtgatgggac tggatgccat aaccttcatt ttttgaatgt tgagttttaa gccaacctct 17160

tcactctcct ctgtcatttt catcaagagg ctctttatcc tcttcacttt ctgctctaag 17220

agcaatgtca tctgtatatc tgtggttatt gatactcctc ccagcaatct tgattccagc 17280

ttgtgcttca tccagcccag catttcacat agtatacttt gcatagaagt taaagaatca 17340

gggtgacaat atacagcctt aacatactcc tttaccaatt tggaaccagt ctgttgttcc 17400

atgtccagtt ctaactcttg cttcttgatc tgcatacaga tttctcagga ggctggtcag 17460

gtgttctggt attcccatct ctctaagaat tttccacagt ttgttgtcat ccacacagtc 17520

aaaggctttg gtgtagtcaa taaagcagta gtagatgttt ttctggaact ctcttgcttt 17580

ttctatgatc caacagatgt tggcaatttg atctctggtt cctctctgcc ttttctaaat 17640

ccagcttgaa catcaggaag ttcatgattc atgtactttt gaagcctggc taggagaatt 17700

ttgagcatta ttttgctagt gtgtgagatg agtgcaattg tgcagtagtt tgaacattct 17760

ttggcattgc ctttctttgg gattggaatg aaaactgacc ttttccagtc ctgtggccac 17820

tgctcaggtt tccaaatttg ctgcatattg agtgcagcac tttcatggca tcatctttta 17880

ggatttgaaa tagctcaact gatattccat cacctctact agctttgttc atagtgaact 17940

cccagtttaa attgccttaa ttaaaatgac taactggttt ttcctttctt ggctgaatct 18000

cagctaagta gcaagagaca taaaggtatg ctgtgaggta cctccaattg gaagattgca 18060

aaatgtgccc agggaataaa gcatatgtca agcgtatgtc ttagtttggg gtatgcacct 18120

gaaaaatagg cagtgatgtc ctagtccagt tgctgtgtgt ctctaatgat gcctgaggta 18180

cattgtcacc caagttttca cacttaattt atcttttttc acataatttt aggattgcaa 18240

agaactgtat tgtcaacaaa gagattttcc cctttactag aaatagcctg attaggactt 18300

cattactatg ctttcctgtc ttctgtcaca ggaaccctac gtcctagggt tcctattgtg 18360

ttctacaaga ttagtatcca tcgcgtctca gatgtgtcac acctgggttt tgtggaagta 18420

atcttggcca tccaaaagct gtgaaaaact agtttaattt actttgcatt ttgccactta 18480

ggaaaatcac tgactaactt tcttgttgag aaacttttat attaaagaaa acacttgaca 18540

cagactaaat tttaattagt ttggaggaag agatatcacc cagtaaaatt catgaaaatt 18600

acttgaatac tacatctgca tatccattta ggcaagccta agtgatagtg aaagaatatt 18660

ggaaaaaggc aaaggattgc tgcttttgtg tgtgggtggg tgggtgggtg gtttggtttc 18720

cagttcatgt tctgttaaat ttacacctcc tattttctta tagcatgcat gcatgttaag 18780

tctcctcagt tgtgtctgga tctttgcaac ccgatggact gtagcccacc aggttcctct 18840

gtccatggga ttttccaggc aagaatactg aagtgggttg tcatgccttt ctccagggga 18900

tcttcctcac ccagaaatcg aacccgcatc tcttatgtct cctattttgg caggcagctt 18960

atttaccact agagcctcag aagccccatt ttcttattaa atcatattaa attcttacag 19020

caaaaggaaa tttcaaaccc aaagttataa tagaaagcac acacctggat actaatccta 19080

gatgttctgc aacattcatg aaatattttt tcacaatata cacaattggt caaaggcgag 19140

aaaatacatt ctgtttctat gagaccaata atttatattc tatatataac aaataataca 19200

ttatacgtct ttcacagtat gacttatttg ttaccatgat gccctcaaat ttcacccata 19260

ttgtaccaaa tgacaggacc tgcttctttc tcataactga ataatattgt atgtactgaa 19320

tgtatatcac attttcttta tcccttatgc atccatggac actaggttgg tttcatcttt 19380

tgctctcgtg actgatgctg gaatgaacat ggccatgcag tcatttcttt gagagagtta 19440

tgtcatgccc tatggacaca cccagaagtg ggatcattgt agcatatggc agttctattt 19500

aacatttttg aggaacctcc gcactactgt ccatagtgct tatacagtcc catcagcagt 19560

gcatgaggat tccattttct acacattttt gccagcatgt tactttttgt ctttgtaata 19620

ataagcagtc taacaggtgt gaggtggaga tttgtcttcc tagtgttggg cctcaggctg 19680

aggtgtccaa tatatggtgc aaatctctca tccctacttc tcatagaggt taacccagcc 19740

cagtccctcc tcttctgtgt cccctcctga gggtatcaag tcactctcct ttcctaccca 19800

atttcatgtg gatcattctg ttttctttta atcttttttt aaaaattatt aaagtatagt 19860

tgatttataa tgttgtgcaa atctctactg tacagcaaag tgactgagtt atacacacat 19920

acacatttct ttctacattt tttaccatgt ggttcactct ttacagtctt ggtaaaagag 19980

tctctctgac agtttccagt tgttttcagt gagaatgact ccccatgtag atgcatttgt 20040

gttgttttcc tgcagggagc tgatctcagt gtccttttat tcccccatct tcacctcctc 20100

atagcaatgt atttaaattt atgcatgtgt gcgtttgctc agtcatatcc aactctttgc 20160

gaccccatgg acggtagccc accatgctcc gttatccatg ggattctcca ggcaggaata 20220

ctggagtggg ttgccatttc ctactccaaa atacatgtga gcatcttgca actcacgtgt 20280

tcccttatat taccaactgt gactgtgcgt gtgatacaaa acatcttttc aacttcctca 20340

tctataggag aatttcaaca aaatttctta actgagacag tggttgagac gagacaatca 20400

caggtactta tcagaacagt gtttggacac agtagtggat caagaagtgt acactatcat 20460

attcaccttg ttaataagcc agtattttgt ttcagctaaa aggtttttca ggtaatatat 20520

ttcatctgaa gcatcaactc ctggaagtca ttagcttgtg aaccagcaaa acacaaacaa 20580

actcttatgc tttccaacta tttttaacca catggaggtt aaagcactct agtttaaaag 20640

atggccttgg agagagtatc atggaagcag atacactacc atacgcaata tagatcccta 20700

atggaaatgt gctctatgac tcagggaact caaacttggg ctctataaca acctagaggg 20760

tggaaaagga agggaggtgg gagggaggtt cacaatggag gggatatatg tacacctatg 20820

gctaattcat gtagatgtat ggcagaaatc gaaacaatat tgtaaagcaa tcatcaagca 20880

atttaaaaaa ataaatgtaa ttaattttta aaagatgggc ttccccagtg gttcagtgat 20940

aagaaaccgc ctgccagtgc aggagacaaa ggtttgattt ctgggttgag aagatcccct 21000

ggataaggga atggcaaccc actctagtat tcttgcctgg ggaatcctat ggaaagagga 21060

gcctgatagg ctacagccct tggtgttgca gagttggaca tgacttagtg aataaacacc 21120

aataaccaca aattatttaa atgccccaac acccagtctc ttcctctcat atccacctgt 21180

gcatatcggc tatgtgatct ctcttaaaaa taccctgtca cttattcaca tacagttaaa 21240

taacattctt ctctccatat ccaatttgac ttggtttaaa gttttttcat gtcactggtg 21300

gctcagaggg taaagcatct gcctgcagtg tgggagacct gggttcgatc cctgggtcag 21360

gaagatcctc tgaagaagga aatggcaacc cactccagta ctcttgcctg gaaaatccca 21420

tggacggagg agcgtagtag gctacagtcc atggggtcgc aaagagtcgg acatgactga 21480

gagacttcag taatgacaaa tcaacctctg cattcatcag gagagcactc tccgtcagga 21540

agactgcatg gcaggactga gaaggttaca tcccaccact ctccacacac atcccacttt 21600

atccactagc aaaaaatggg taagccaaat ccacatttta ttaaagtctt caagagctga 21660

gaccacaagg aaaccaagag aaaacatacc caggtttgag tggatatcat tcgaatcagc 21720

agaaatcaga aacatgtgga acatatcatg cggaaacttg ttacatctgt tcacggagca 21780

gctacaggtt gtcctgatgc taatggaggg gctctcctag tataccataa accagcagtc 21840

tacttgagac tacatggcct gaagctatag agcagggtct gcagaagtac ccagtggaga 21900

ggaaggtcaa cccatttcta gtttgtttcc agggaatttt tgctgagatc atgttggtgc 21960

agtgaatcta ggaatatgct gcaaagcaga tgggcaagac atgactattg aagggtttgg 22020

agcacaaagt ccttatagag gatgaggcct gtgtggtgag caggacctta gatgctttca 22080

ctgatctcaa ctcctggttt tcattccact gtgtatttcc tcccatcaac tgtgagctat 22140

agtaaggaga tgaaagtacg ttatctgtag agacatttat taactctgaa aatggaaagt 22200

gtatcacatc attggtttac atctcttgcc acacagattt catgattgat acgatggatt 22260

ttcagctatc aaaaccacac tttgagtggc aaaattgagg ggggaaatgt gtaattttat 22320

gttaagatcc tgcaactttg aagtaaggaa caagagtaca tggaattacc tactaaagag 22380

aagagggaac cagacctttc caaggacatt cacttttctc cttttcctgt tctgttaata 22440

ttaacaagtc cccagagatg aggacaacat ggacctaatg atgagctttg tgcctcgagc 22500

tgtaaggtta tactatttta aataagtggc cagtgcctag agctctttag tttgccagtt 22560

ttgataccat gaatccacac ctatgtgccc atcctttata gcaatctgca tcagaaggtc 22620

cagcctgagt ctgtcttcct gctcaacctg aggagagact ctgagacttc atcacacacc 22680

aggcaggaga gggtgagtga gtctatgctc cttggtcatg ctccagcaga gatgcagccc 22740

agcccagtaa gtgctaggga gagagggatg gtgcagggaa tgttggaagc aatctatgca 22800

ggtttggaat aaagcacttt gaagttcata ttcttggttc tggttgagat gtgagaactc 22860

tcattcagag ttctgctttc accctcaatg gcctggctct ggctggcatc cctcccacta 22920

gagaagcaaa tgcacctctg taaacattcc ccttgttcag ttgtggttca gccactcagt 22980

tgtgtccaac tctttgtgga ctgcagcacg ccagtcttcc ctgtccttca ccatctccca 23040

gactttgctc aaactcatgt ccattgagtt gatgatgcca tccaaccatc tcatcctctg 23100

tcccccttcc cactaacact tcagttcatt acgggatcag tgatgaggat gaggacgagc 23160

atgatgagga tgatatgaac cataccatcc tgtcctgtta ttgaaggctt cctaggtgtc 23220

gggctctgaa gtataagttt tatatttcct tcttgagtac atctgcataa tttctctcca 23280

atcatatgta tacacattac tattatttta ataagaggaa agaagtgaaa tattttatgt 23340

aacatgtacc agtttatggg gctttcccgg tggcttagtg gtaaaaggat ctgcctgcca 23400

gtgcaggaga ctcaggatca agtcccgggt catgaagatc ccctggagaa ggaaatagca 23460

accgacccca gtgttcttgc ctgggaaatc acatggacag aggagcctgg cgggctacag 23520

tccttggtgt ctcaaagagt cagacacaac ttagggacta aacaatagca accagatcag 23580

gagttaaaga atgataaaat catgtttgaa gtcagtcatt ttggacagac tgattccaga 23640

tgcaaggtat gtgggcaatc ctctgccacc ccaaaccatc atgtcactca cttccctgtc 23700

agtcctggca gagactccct gctccctgct cactgttaca ggaacacttg tacttggttg 23760

gtgggaatga ataatgatac agccattgca gaaaacagaa tggaggttcc ttaaaaaaat 23820

taaaagctgc aactaattga aatagaacta ccataggatc tagcaaccca cttcagggta 23880

ggtgccaaag aaataaaatc agaacctcta agggatattt gcattccctt gtaatcatca 23940

tattacttga atagtgaaag tgtgtttatt gaagatgaat aggtaaaaga tgttgggtgt 24000

atatataaac aatgggatat tactcagcca taaaaaggac atcctgagat ttctgacaac 24060

atggaatgac ctggagaata ttatgcctat tttttaactc atttactata attggaggat 24120

aattacttta caatgttaca atggttcttg ccaaacatca ccaagaatca aaatttcaag 24180

agagttctta aattatatta tcatttactc attgacagat gattaataaa ggacaaaaat 24240

ggtatggacc taacagaagc agaaaatata atgaggtggc aagaatacac agaactatac 24300

aaaaaagatc ttcatgaccc agataatcag ataatcaccc agataatcac caaataatca 24360

tgacccagat aatcttcatg actcagatga tataatcact catctagagc cagacatcct 24420

ggaatgtaaa atcaaatggg ccttaggaag catcactatg aacaaagcta gtagaagtga 24480

tggaattcca attgagctat ttcaaatcct aaaagacaat gctgtgaaag tgctgcactc 24540

aatatgccag caaatttgga aaactcagca gtggccacag gactgaaaaa ggtcagtttt 24600

cattccaatc ccaaagaaag gtaatgccaa agaacactca aactactgca cagttgcact 24660

catctcatgc tggcaaaata atgctcaaaa ttctccaaac caggcttcaa cagtacatga 24720

accatgaact tccagatgtt caagctgaat ttagaaaagg caaagaggaa ccagagatca 24780

aattgccaac atctgttgga tcatagaaaa agcaagagag ttccagaaaa aaatatctac 24840

ttctgctttg ttgactacac caaagccttt gactgtgtgg atgacaacaa actgtggaaa 24900

attcttcaag agatgggaat accagaatgc ctgaccttcc tcttgagaaa catgtatgca 24960

ggtcaggaag caacagttag aactggacaa ggtacaacag actggttcca aataggaaaa 25020

ggagtacatc aaggctgtat attgtcaccc tacttattta acttctatgc agagtacatc 25080

atgagaaacg ctgggctgga tgaagcacaa actggaatca agattgccaa gagaaatatc 25140

aatcagctca gatatgcaga tgacatcacc cttatggcag aaagggaaga agaactaaag 25200

agtctcttga tgaaagtgaa agaggagagg gagaaagtgg gcttaaagct caacattcag 25260

aaaactaaga tcatggcatc cagtcccatc acttcatggc aaatagatgg aaaaacaatg 25320

gaaactgtga gattttattt tcaggggctc caaaatcaac gcagatggtg actgcagcca 25380

tgaaattaaa agatgcttgc ccctggaaaa aaaaaagcta agaccaacct agacagcata 25440

ttaaaaagca gagacattac tttgccaaca aaggtccctc tagtcaaggc tacggttttt 25500

ccagtagtca tgtatggatg tgagagttag actatcaagc aagctaatca ccaaagaatt 25560

gatgattttg aactgtggtg ttgaagaaga ctcttgagag tcccttggac tccaaggaga 25620

tccaaccagt ccatcctaaa ggagatcagt cctgagtgtt cattggaagg actgatgctg 25680

aagctgaaat tccaatactt tggccatctg atgcaaagag ctgactcttt tgaaaagacc 25740

ctgatgctgg aaaagattga aggtgggaga agtaggagac aacagaggat gagattgttg 25800

gatggaatca ccaactcaat ggacatgggt ttaggtaaac tccaggagtt ggtgatggac 25860

agggaggcct ggcatgctgc agtccatggg gtcgcaaaga gtcggacacg actgaactga 25920

actgaactga actgtatagc atagtgaacc cttctcaata ttctgtgaga ctaatttggg 25980

gaaaaaaatc taaaaaccag tggatatgta tgatgtataa ctgattcact ttcttgtaca 26040

cctgaaacta aaataccagt aaatcaactg tattccaata atttttaata atattcttta 26100

atatggcaat gtaattccat tagataaaaa ctcaaaaaca aagattcatt caaattagag 26160

ataaaaaatg taaatcttca tgatataaaa tgccattcta agcaaggagt aaagcaatga 26220

aaacaataat ttatacctag aggcaactaa ctgagaaaat ggaaagtgta tcacaccctg 26280

gtaaaaagtc ctaaaatgcc tagatttaat gataaataaa gagacttaca aatatcaaaa 26340

atcacacatt gtgtgattaa attgagaaaa aagtctgaat tttatgttat cctcctgcaa 26400

cttggaagta agaagtaaag cggagaggtg cacaaccacc tgaggagagg agggacattt 26460

ccaggacatt cacttttctc cttttccttt ctgtattaac aaatctccag agattgggat 26520

aacatggacc aactgactaa tgatgagctt tgggcattga gccctaaggt cataccatta 26580

aaaataattg gccagtgcct ccagctcttt agtttgcctg ttctaatatt gtgagtccac 26640

acctatgtct ccatcctttg taggaacctg catcagaagg tccagtctga atatgtcttc 26700

ctgcccatcc tgaggaggga ccctgagact tcatcacaca ccaggcagga gagggacagt 26760

gagtctgtgc tccttggtca tgctccagca gaaatgcagc ctagcccagt aagtgcagag 26820

ggagacagtg gttgacagcg ggactatttc agcattattt cctcctcaag agcaacaaat 26880

atgatagttt tcatagaaag gtaggaagac acccatctac atggttttgg aatagtgact 26940

tttgaaatat atgtatttgg ttctggttgg catatgagaa ctctcattca gagttctgca 27000

tacaccttac tggttctggt tctgactggc atccttccca tcagagcacc taatgggtct 27060

ctgtaaactt tctttcacac atttaaataa aatgaaatgg tgatgaacag atgatgaaaa 27120

tgaggaggat aaagctcatc ataacatcat gtactgttat tcaaggcttc ctaagtatca 27180

ggctctgagg cacgttttat gtttcttgct tgattctgtc tgcataatct ttcccaaatt 27240

attagtgcac acattattat tacttaaata agaggaaaga agcagagcat tttatgtcat 27300

atctaccact tcatgttaaa gtgttaaagc caagtctgaa gcctgtcaat ctgggcagac 27360

tgactccaga cacagagcat cagggcagac ttcccctttt cacccaaaca gtcctgtcac 27420

ttcctggcag agactccctg cttactgtgc ccttcccctt ggagttgtct ggagggcaca 27480

gtgttgggca ggtatcactt gccacatata ctataggaag aatgtctgag atcacagatg 27540

aagccataga aataactggc acctgatact ttagggtaga tctctgttga tgctgttctt 27600

tgatttcact tcacctgtcc actttctcct ctatagttag aatgagctaa gtcaatcatg 27660

aaggagacag aaaatcagtc ttagggccca gtcctattaa ggaaaatgaa aacatttaaa 27720

ataatgtact ccagatgtat ccatagttat acctctctac ctctgctgaa aactttcaga 27780

aagtctataa acaatgaaat ttagggcatg ggactcagag aagaaaaaca tttatttttg 27840

aatcctgttt gcttcactca ttcttagtgt actgtggggt aatttcttag aatctttgag 27900

atttaggatt atcaactgag ggattcctca ctaatacaga accactggat tgggactaag 27960

caaacaggct caccctcaat gcaataacta tgtgatggat gcaaaagaaa gggaaaaata 28020

catatttgat tttgttacta ataatgataa ggctatttgt catatcatca ggtttaaata 28080

tctctagatc tgatgttgtg gtttccttac atccctgaca ggacatttta gtttctacaa 28140

gtacattctt cctagactaa gagaactgag tgaggctcat ttatttctga ttccccaaaa 28200

cctgtatctt gttggtgcag aagagacacc aaggataaaa atgcccagtt aagtgttgaa 28260

taaactcttt ttttcatgga aacaacatgt gagcaatatg gtccacagag tggaccaatt 28320

atgtcagtgt tttcatcctt ttcagaaagt cccacaggtc tagagaaaag ttaaaacact 28380

ggttgtttct gtttcatggg cttcctttga gacttgttat tttccattta agagcatctg 28440

caacattcaa tcattgagaa gtcattgaag aaattttggt aaatatgggg agaaagagtt 28500

agaaaagaag aggacatgca cgttttattt cacgtgccta gccatatttt aggatttgat 28560

gttttctgtg ctccacctct gagataggaa aggctttgat ctctctgtca tatactccat 28620

gatacatcat tacattttgg gctttgcact taattaataa acatgcatgt gtactaagtc 28680

acttcagttg tgtccagcta tttgctaccc catggactgt ctcctgccag gcccctctgt 28740

caatggagtt ctccaggcaa gaatactgga gtgggttgcc aagccctcat ccaggggatc 28800

tttctgacct gcatctctta tgtctcctgc attggcaggc aggattttta ccactagcac 28860

ctcatggaaa gccctattaa taaacacatt atcccaataa tgattaaaaa tctttataca 28920

actgaccaaa tatttattgc ttccaactac cctctggtgc cacatgtatt ctagagtagg 28980

tgttagatct tcatgtccaa gaagagaagc acagagagcg tgagataatt gcacatattt 29040

cacttacaag gatgaaagac ccaggactct tatcactgat ctgctttttt ctgcaccagc 29100

tctcagtcat ctccactgta gctaatgtgt ttcttttcaa acctacttgg ctcccatagg 29160

tgataggaag gtagataatt acttcatcct cttttggttc agtgagaatc tgtgaatcat 29220

gggactgagg tcgcagttta cagaggtcat aatggcacta acatggatga ctgtaaggtt 29280

ttcagtgaat agtcatgcaa actgcagtcc agctttgaaa tttaaggttg agaagatttc 29340

ctatacaagg cgaaaatggc attggatgca gttgacagaa tattatattt gcactgaaac 29400

gagtacaggg aattagcttt cctggtggct cagacaacaa agaatctacc tgtgatgcag 29460

gaggcccagg tttgatccct gggttgggaa gattccctgc agaaggaagc ggcaacccac 29520

tccagtattc ttacgtggag cctggctcct ctgtatcctt acagaggagc ctggcgggct 29580

ccagtccatg gggtcgcatg gagtcagaca caactgagca cacacatacc aaactggtta 29640

tgcacccact gagagaactt aactaattac cactcacatg ttatgtacta atttccattt 29700

gtctgaaaca gagaaatgta cgagaatgtt cattggattg ttggaagcag gaactgatat 29760

aaataaaaat aacatatgca atgtaaacta aatatttatt taatatatca gttcagttca 29820

gttcagtcgc tcagtcatgt ccgactcttt gtgaccccat gaattgcagc atgccaggcc 29880

tccctgtccc tcaccaactc ctggagttca ctcagactca cgtccattga gtcagtcatg 29940

ccatccagcc atctcatcct ctgtcatccc cttctcctcc tgcccccaat ccctcccagc 30000

atcacagtct tttccaatga gtcaactctt cgcatgaggt ggccaaagta ctggagtttc 30060

agcttcagca tcattccttc caaagaaatc ccagggccaa tctccttcag aatggactgg 30120

ttggatctcc ttgcagtcca agggactctc aagagtcttc tccaacacca cagttcaaaa 30180

gcatcaattc ttcagcgctc agccttcttt acagtccaac tctcacatcc atacatgacc 30240

acaggaaaaa ccatagcctt gactagacag agcttagtcg gcaaagtaat gtctctgctt 30300

ttgaatatgc tatctaggtt ggtcataact tttcttccaa agagtaagtg tcttttaatt 30360

tcatggctgc agtcaccatc tgcagtgatt ttggagccca aaaaaataaa gtctgacact 30420

gtttccattg tttccccatc tatttcccat gaagtgatgg gaccagatgc catgatcttt 30480

gttttctgaa tgttgagctt taagccaact ttttcactct cctgtttcac tttcatcaag 30540

aggcttttta gttcctcttc actttctgcc ataagggtgg tgtcatctgc atatctgagg 30600

ttattgatta tttctcccag caatcttgat tccagcttgt gtttcttcca gtccagcgtt 30660

tctcatgatg tactctgcat agaagttaaa taagcagggt gacaatatac agccttgaca 30720

tactcctttt cctatttgga accagtctgt tgttccatgt ccagttcgaa ctgttgcttc 30780

ctgacctgca tacagatttc tcaagaggca gatcaggtgg tctggtattc ccatctctct 30840

cagaattttc cacagtttat tgtgatccac acagtcaaag gctttggcat agtcaataca 30900

gcagaaatag atgtttttct ggaactctct tgctttttca aaatatatca aattatatat 30960

tactgcactt aaatgtgtta aatttgttaa agtgaaatta atttatataa atttaccaac 31020

tgaagaaaat gctgtctctc cttatataat aatgtgtgtc agaaaattgt ttgatgtagt 31080

tgtatgtact tcacaagaga cttaaccaaa tggtgtggta atgaagtgca ttcagaggca 31140

gtatggatat tattttttta gggaagattt tttaaaagct ggttgtatgt gtgttagtca 31200

ctccatcttg tccaactctt tgcaccccca tggactgtag tccatgaggc tcctctatcc 31260

atggaattct ccaggcaaaa atactggagt gggttgccat ttccttctcc agggcatcta 31320

ccttacccag ggattgaacc caggtctcct gctttgcagg tggattcttt actgtctgag 31380

ctactacaga aaccctagaa gctggttatg ggtatgtaaa tgaatattga cattatgaaa 31440

acatttacaa gaaaacatat gtcaagttca tcacactggt tgctttgggg acgtgtatgc 31500

agggaagtgg gattgaactg aggttccaag agaagttaaa ttttatcatt aatgtttcaa 31560

atttctacaa tatgtgggat ctgaagaaat attattacat gtttatattt taagaataag 31620

tgtggtattc atgacagtat taatcaataa tattcatgga gacttttcta agtgttgggc 31680

aatattcatt ctgaggcagt gttgatcaag ccctggttca cagaagggag acagacactc 31740

atctatagag gtgctgaaat aggttctagg ctgaggtcag ggtaactaag catttttaaa 31800

taagcacatg taaataattg gtgaaatgga actgaaaatc actgctttaa tcaatataaa 31860

tgatttttta aaactttctt gcaatggtcc tattggtagg ttcaattact atcacaattt 31920

catagataag gaaactgctc agatgtgttt gcattattct atttaaatct ttttcacaac 31980

acactccctt gtcattgcaa tattttttga aatagaatat ggatatatgt ctgttgacag 32040

atgaacaggt aaagaaaatg tggagtgtat ataaacaatg ggatattact caggcatgag 32100

gggagataga gaggaatcaa catgaaccct gttaattttt tttgttctgt tacatttatg 32160

tttttaatta atttacttat tttaatggaa gataatttaa aaattgtgat ggtttttgcc 32220

atacatcgac atgaattggc caaaggtaca catgtacaac caacaaggac tttttgtata 32280

gtacagggaa ctctgctcaa tgttatgtgg tagtgtgaac ggaaggggat tttgagggag 32340

aatgggtaca tttatatgta tggctgagtt cctttgctgt ccgcctgaaa ctatcacaac 32400

attgttaact gactacaccc aatataaaat aagatttttt tttaataaga agtttttaaa 32460

aagagctgca gttgtgaggc ctagtggtgg agaaggtaga gaaatgttgg tcaaaggata 32520

taacttttgc taaaaaatga atatattctg gggatctaat atgcagtctg atgactataa 32580

ttaataattt catattgtgt tcttgaaatc tgctatgaga ggtttgttta ccttacacca 32640

caaacacgtg agcacaaata gaagttatgt aaggtgacat atacattaat tactttgatt 32700

gtggtagtca caatttgtat tgtagtaaat catcattttg tacaagtttt cataaactta 32760

tgtggcaaaa cccaaatata agcaattttg tcaatcactt caataaactg gaaaaaaaaa 32820

gaaaaacaat aaaggagtat tagttcacca gcaaagaatg gactccaaaa aatgtaaagt 32880

ggtctcacac attagacatt taactgagct ggtatgaaaa caaacactgt atacgattaa 32940

aaaaaaaaat ttcaagaacg tttctaaaat ttcattatca tttaatcatt gacaatgctc 33000

tgtatgctaa tgtcataatt atagtaatta attctgtatg atcacgtgcg ctgaattaaa 33060

ccatacagat caaattttta agtccaccaa agtcaaatga agtatatact aaatgatttt 33120

ttaaatgcaa ccctgataag tgagtaatca cttatttcat gaaggaggga agacaggaaa 33180

accaataata aaactctgaa tatcacttaa atatgttttc tgattgtaaa cattcatttt 33240

tagtttagaa tccagatgaa caatttcttc atttcataga gatgtaaaaa tattccaaat 33300

aaaggaaata actagaattt aatttaaata agatttatac aaatatgttt aatttctttg 33360

aattatattt tttcttaaag gaggaaggga atttctcttt ctgtattatt tctaaagaag 33420

taggctgtat ttaactgaga tcatagatat taattccaat gttcttgtcc cctttcactt 33480

ttatgtggag ttttataatc taagagtttg tagacagatg tgtctaacag aaaccaagga 33540

ggaaaaattc taagcaaata gaatttgtca tagtgagaaa tattgctgga ggaacaagag 33600

tgtataatct tagaaataaa ttgcttgaat ccaaaatatg gacacagtct caacgcccgt 33660

ttacttattt cctttgtttc cagccctgtt tcctttttcc aaaggtgtct gagatacaca 33720

gaactggaaa tttaacaagt gtctcagaat tcttcctcct gggcctctca gatgatgcag 33780

aactgcagcc tttgctctgt gtcctcttcc tgtccatgta cctggtcacc gtgttgggga 33840

acctgttcat catattagcc gtcacctctg acccctgcct ccacaccccc atgtacttct 33900

tcctctccaa cctgtcctcg gttgacatag gtttcatctc caccagagtc cccaaaatga 33960

ttgtggacat ccaatctcaa agcagagtca tctcctattc aggttgcctg actcagatgt 34020

ctatttttat cctttttgca cgtacggatt gtatgcttct tactgtgatg cctatgacag 34080

atttgtggcc atctgtcacc cactgcacta cattgtcatc atgaacccat gcctgttttg 34140

ctccttagtt ttggtgtcct tttgtgttag ccttttggac tcccaggtgc acaatgtgac 34200

tgtgttacaa cttacctgtt tcaaggatgt agaaatttct agtttcttct gtgacccttc 34260

tcaaccgctc aaccttgctt gttctgacac tttcaccaat aacatagtca gctatactat 34320

ccgtgccttg actggttttc tatctcagga aacattttct cttactataa aattcttgtc 34380

tccattctga gagtcccctc atcaggtggg atgtacaaag cctttgccac ctgtggttct 34440

catctggcag ttgtttgctt attttatgga acaggtcttg gtgtgtacct cagctcagcc 34500

atctcacaat ctcccaggaa ggatgcagca gcctcggtgg tgtacactgt ggtcaccccc 34560

atgctgaacc ccttcatcta cagcctgagg aacaaagaca tccaaagggc cctgtggagg 34620

ttcctggtag cacaaactca acttaggacc ttcgcaaccg atttgcatgt atactaaaaa 34680

atgtagcaaa actaaacctg gagatctaaa aattccacct ctctcatgac accattttta 34740

tagctcttat ggacttcatg tctctcctca ctttctacct gaatattact tctctttacg 34800

gtttatcccc ctgccagtgc aggagacaca agaaacacag gtccaatccc tgagtctgga 34860

agatccctgg aggaggaaat ggcaacccac tccagtattc ttgcctttga aatcccatgg 34920

acagagaagg ctggtggtct acagtctgca gggttgcaaa gagtcaaaca agactgagca 34980

catgcaaaca cacatggttt aacatgacag tggggaattc taagagtctt ttgtatcaaa 35040

atcactcctt gaatgaatct cattaatatc tatggagatt ctatatttta tctttggtag 35100

cccacgcatc ctcaaaagat gaataattct ccaggcaaga acactggact gggttgccat 35160

ttccttctcc aaggcatgaa agtgaaaagt gaaagtgaag tcgctcagtc atgtctgact 35220

cttcatgacc ccatggactg cagcccacca ggctctgcca tccatgggat tttccaggca 35280

agagtactgg agtggggtgc cattgccttc tccggtggag aagtttagaa gacttaaatc 35340

tgttccagag aaggctttga agagaagggg cttatttggt tttatgataa gagggaagag 35400

gtaagagaaa aataatattc aatatttcat gagtttcact ctgtgtcatt cacagaattg 35460

tggagccata cattgcttca tcagttcagt tcagttcagt tcagtcgctc agtagtgtcc 35520

aactctttgc aaccccatga actgcagcac gccagaggta cccaaatctg aagtccgtaa 35580

aaagcatggt tatggaaaaa tcagcaagaa gcaaatttcc ctgacaggta acgtgttgat 35640

tgctcgatct cttgggaatg tatggctcca caactctcag catgttaact gaagccttgc 35700

tgagcttcac aaaggtgcta ttgaagatct gccggaggcg aaggagctgt agcacctttt 35760

gaacctttgg gccacgctgt tgacacctct gatcctgacg acaaatgcca atttgggttc 35820

tgcaggtaca tagaagttgc cagctttttg ttccatccta gccattcgaa tttcagttct 35880

gtacatctgc atgtattcct tgcggtaatg cttagctttt tcatagacaa gcttcctcct 35940

tgcctttcaa agcatctttt gggcaaactt ctttctcagg tgcttaatct taagctctgt 36000

gaaattcttt tgctttttct taggggtttc aggcacagta ggaacctcct ttttttttct 36060

cttctgcaca tttcatggtt ccagctggaa aagaggtatc tcaaaatttg tgtgtgtgaa 36120

gagtaaatac gttggagcta taaagtttca aagagttccc tgcacagagt aacaaacagt 36180

tcaataaacc ataactattt cagtggaatc aaactattaa tatgcatata ttgctaacaa 36240

tgagctgtac gtattggtct catatttttt tttttttatc tttattggag tatagttact 36300

ttacaatgtt gtattagttt ctactataca gaaaaatgaa tcagttatac atgtacatac 36360

ttccttccca ttcaggtcac cacagtgcat taagtagagt tccctgtgct atacagtagg 36420

ttatctattt tatacacagt ataagtagta tatatgtgtc aatatcagtc tcccaattcc 36480

tcccacccca tcgtttcccc ttggtatcca tatatttgtt ctctacacct gtgtctctat 36540

ttctgctttg caaatatcta tactattttt ctagatttca tatatatgaa ttaatataca 36600

atatttgctt ttctctgact tacttcactt tgtatgacat tctctaggtc catcaatgaa 36660

cactggggtg catgtgtctt tttcaattag gtcttatact ttattaaatg gattctttcc 36720

ttccctgtaa ccacactcct tccgtaagat aaggagttca tattctgtgg ataattggaa 36780

agatatctgg agcttgccta gaagatggca agtgaaggtc actccaaaat gctgtctcag 36840

tctcagctga ccctagcagt tccctgtcta accacaaata agcctgcttt ctctaactgg 36900

ttcttcagaa ttctttggtc actggaggca atatagtata gttataaaaa tcatgcactc 36960

tgaattcaga cgtagtttca ctacatgtat aacaccggcc aattatttga gctcatgtgt 37020

gctgtttcac tatttgtgtc aagtggataa gtatctgcct cataaacttt ttgtgacaat 37080

caaaaagatt aatatataga aatatcttga gtattaagat agtgaatgat acagagtgaa 37140

actcatgaaa tattaaatat tatttttctc ttacctcttc cctcttatca taaaaccaaa 37200

taagcccctt ctcttcaaag ccttctctga aacaatttaa gtcttctaca cttctccacc 37260

agagaaggca atggcacccc actccagtac tcttgcctgg aaaatcccat ggacggcaga 37320

gcctggtggg ctgcagtcca tggggttgtg aagagtcaga cacaactgag cgacttcact 37380

ttcacttttc actttcatgc cttggagaag gaaatggcaa cccagtccag tgttcttgcc 37440

tggagaatcc cagggatggg ggagccgggc gggctgctat ctatggggtc acacagagtc 37500

ggacacgact gaagtgactt agcagcagca gcagcaatag caaacttctc aacggtatgg 37560

gacatgggta tggccccatg gcatgtgaca tcttagttcc aatggtatta gttccctgac 37620

cagggatcaa acctacaccc ccatcattgg aggcacagag tcttaaccac tggaccacca 37680

gggaagtcct tgagaaggaa gtgtttctga tagcacagaa aggatgcagt cccaccccgg 37740

ccactgcagt agcacttcct atgtgcattt ctgatacact aaactaagga ccagaaagct 37800

taaatgactt ttttctaagg tcacagaggt aataagttga aaagtgaaga ctagaaatca 37860

ggtgtcttgg ttcccagacc agttttcttt tcattccaac aagaaaaccc tgtgacctta 37920

tgacaatagg caacataaag gtgtttctct gcatacatct caaatgcatc gcaactgata 37980

atagtagaat attctgatgg gatggttcta taagcaaact acacaaaaca taagacaagc 38040

agtgacataa gcatctatca caactagcta ggaaaatatg agaaaattaa acccaaattc 38100

agtagaagga ataagaccaa aaattaaaga attataagta gaataaaact gagagtttgt 38160

tcttcaaaaa caattaaaat atctaaatcc ctaccaagat gaagaaaagg gggactacat 38220

aaattaccaa gatcaggaat gaaaaaggag atattattat cattacagct tttagacatt 38280

aaaaataaat tataggactt tattaataac tttagaccga caaatttgga aagttatgga 38340

atggacagat acaatatagc aaaacaaaca caaattttat ataatctttg tctatttata 38400

cattcaatat aaatcaccac tggagtagga aatggcaacc cactccagaa tccttgcctg 38460

gaaaactcca tgaacagtgc cacctggcag gctacagtcc atggggttgc aaagagttgg 38520

acaaaactga gcattacaca cacacacaca aatttaccag taaaactatt ttacaaaagc 38580

ttcctgtcaa gaaaacccaa atatagattc cctggtgaac ttttccaaac atataagtaa 38640

tagatataga tagttcagat ttcatataaa gtcttcagaa cagtttccaa cttttatgag 38700

gcagcatgaa cctgatacaa agcatactga cataaaagaa attacaagac aaacctcatt 38760

aacacagaca cagaaatatt ttttcaaata gcattttgaa tctagcaaaa tgaataggtt 38820

aataagctaa tctataacat tcccaagtat ggtctattcc aggactacat gttgtcttac 38880

cacttgaaaa ccaaggaatg tagtaattct ccacgttaag agaccaagga attattttta 38940

aagaccctga catagacaga aaaggcacta gataaaatcc attatccatt catgattttt 39000

aaaactcttc ccatatatat atatatatat atatccttat gattgattat ggatgattta 39060

tgttattgta cagcagaaac caacacaacg ttataacgca attatcctcc aataaagatt 39120

tttttaagtt acaaggtatt ttttaaaaaa catcttcaca attgaggaat agatagtaga 39180

acagaattcc tttccttttt aaaagttatc tatgaaaaac ttataactat tattaactta 39240

atggcaaaat atggaacatt ttcccctaag atcaggaaga atgcaaagat gttcactttc 39300

atcatttcta tttattacta taaagaagaa aaagaaaggg taaaatttag aaatcaagac 39360

tttcttttat tggtcttgat aagattgtat acaatgaaaa cccaaaataa ctcataaacc 39420

atttaaataa ataattgaat ttataaatgt catggataaa agatcaatgt acaaaaggaa 39480

actgcaattc tatgtgctag cctccaaaat caaaaattaa attaaaatat ttacaaagga 39540

agacatacca caagcctaga aataaagtta ttcaagacct ctacaataga aactacaaaa 39600

aaaactgtta agaggaatta aagctgacct agataaatta acaattatat taggctcatc 39660

atagtgttgt aaaaacgtta attttattca tgttattcta taaagtcaat gcaattccaa 39720

gcagagtccc aaaagatttg tgaaaattgc taaggagatc ctaaaattaa tatagaatca 39780

agtgggaaat agagaaggca tgacaatctt gatgaataaa aaggttggaa ggctcttgct 39840

atcaagtatc taaaaatatt gcaaaattat gtaaagactg agaaatcatc ttcagcaatc 39900

aaaagagatg gtaacataat caaacctttc tgaaagattt cttgaactat ggctggctgg 39960

gaattcccag acttcatagt tccctcccac tgctaagatc agagtaagca tattgtgttg 40020

gctaatgtta ttattacatc tattactata ccctatctgc ccctgctgcc ttctcccttc 40080

accatataca tcatgaaatc aaccctgaat attcattgga aggaccatta ctgaaactga 40140

agttccaata ctttgaccac ctgatgcaga gggccaattc attggaaaac accctgatga 40200

tgggaaagat tgagagcagg agacgggggt gacagagaat gagatggttg gatggcatca 40260

ccaactcaat ggacatgagt ttgagcaaac tccaggagat agtgaaggac agggaaggct 40320

ggtgtgcttc agtccattgg gtcacaaaga ttctgacatg acttagcaac ggaataacaa 40380

gattcagtga gaagccaatc ctaaaccaac caaagaattg tatttctctg gccacaatga 40440

attggtaaca gttggcaagt aagccaatca gattgaactg taacaggtat attggaattg 40500

gtcagaatga tcatcactct cttctccctg acacatcaac ctggaaagaa agcagctagg 40560

atcttctgag ggccactgtg gagagaaaga gcccaccagg aagaatagtt agtgcagaaa 40620

gatgtacaat caaggaatgg agacagaatt catccagata acatcttttt agctccagga 40680

tccagccatc tctgcgatta gttataacca aggatttttc tgtaatcaaa ctatttaatt 40740

ctctttatag tttacaaaaa tgcaaagaat gaagacaagc cagagactgg gaaaaattat 40800

ctgcaataca gtttatatat agtatatacc tgcaatacag gtatatatag tatacataat 40860

ttacatatat ataatataca acagatgttt tatgtatatg tatgtaacta gatagagaca 40920

tacacataca tttaaaaagg ataagtaacc agaggaaggg cttcccaggt ggtatagtgc 40980

aaaagaatcc tcctgccaat gcaggagaca caggagacat gggttcaatc cctggctcag 41040

aagatcccct ggagaaggaa atggcaaccc acttcagtat tcttgcctgg gaaatcccat 41100

ggacagagga gcctggtgga ctacagtcca tgggatcata aagagttgga catgactgag 41160

tgactgatca tgcacaaaag tggcagtttt aagacatggc tcccaaattc ttttatagtc 41220

ctctcatgaa agtgaagttc atatttcctc ttttgaatca acttataatt ctaagtatga 41280

attgtagctg aaactattaa tacctgtttc taaaaacttt gttacccagc ctattgaaat 41340

gtcagtttca aaatggggcc aataaaaatt acaattttct ataacatttt tagatctcag 41400

ctatgtattc tcacatgctc ctacattcac tgccacaggt atagaactct tttcttatct 41460

ctgtggactc tacacaattt aatcttgatt tcacgcaacc agtttctcca gggactaggg 41520

gtggggaaaa cggggagata gtgatcaaag gatgcaaact gccagataaa agatgaataa 41580

attcttgaga tctaactata gtcagggctt acctgacttc cctggtagct cagacagcaa 41640

agaatccacc tacattgtag gagacctggg gtttatctct ggtcagtaag atcccctgga 41700

gaagggaaca gctaactcac tccagtattc ttgcctggag aattccatgg acagagggga 41760

ctagaggtta cagtccctgg cattgcaaag aactggacat gactgatcaa ctgagcacac 41820

acacacacac acacaaccat aggaaacctt tcaaacagaa aaggaaccaa aaatgggtga 41880

aatgcttcaa caggtatccc ccagaaagaa gacatccagg gagttgcctg gttgcctagt 41940

ggttaggtgc tgggaactgc cacgggagat cccacccatg acagggtcat gtggaagaga 42000

actgttaagc aaggcttcag aacttaaggg gctccctagg ctttctcgag catctacccc 42060

aaaaccagaa tctgtctgtt ttactgtttc atgactttca ccaactcctc tgacattaac 42120

agggggctaa ccctgaccac ctttctctgg aaaaaatcaa cttagggtta tagctaataa 42180

gtttcctgga catgagagga atatttcaaa tcaaactccc tctgttagca ttctagcttg 42240

cttggcaggt atatccagac tcttatagct acgcatgtga ttatgtacag cctcccaact 42300

gtgagaggca cagaaagcct aaaacataga gcctttaaaa gagttaaagt tattagagta 42360

atgctagtgc tgatgtagga tttcattttt gggccaatgc ttgttgctaa gttcccatat 42420

ctcttatcca ctgtgcacct gggaatgcat tagttaacat agtgagaatg caagaaaaac 42480

aagtgtagcc ttgaatttaa ccatatcgga cttttgagct aattggttct ttcttgtaac 42540

tcactgcacc tttgctctat gaaaaatgta actctgtttg gcattttctg aggctgacat 42600

agatttaaaa tataaagaaa aaacactttg agggaaaata agttttctgg ttgagcagcc 42660

tttatcaaaa gagggtcata aaatgttcac aggcctccaa ggccagaaga tactgtatac 42720

aacatctttt gtggaaaagg tatacagaaa aatcctggtt ttgataaggg caaaactgct 42780

ggaatgtttg ggctgactct gtatgacctt gcctctttca tttcccttta tgtaaaggca 42840

aggtataaaa gtaccttttg aaaaataaag cttttggggc ctcgcagaag cttggtcacc 42900

ccgtgttttt tcttctttta tctttctttc tctctctctc tctctccctc tccctctctt 42960

tttcaggctg atcccttgga gcatgggggc tccctgcgtt cacttatctg cccaggtttc 43020

taagacctga acgggaagac attctgcatc ttcactccct cgggagactg ggagggcacc 43080

tgtggcctct gtgaacaggg caaacttctt gtcttgaagt tttattggtt ctctatgtaa 43140

accaaggaat atcagcctct ttctctcctc tattttctta tctacaacat tctttcctta 43200

tctctctcta aatcaatcgc cgacgctgtt tctccttcgg gtttccctgg atcctgcggg 43260

gcctggaccc cagcagttag gattccaggc tttcactatc atggcccagg ttcaatctct 43320

ggtcagggaa ctaaaaacct gcaagtcaca cagccaaaaa acaaaacaaa aaaaaacaaa 43380

ccagatatcc aaataatcaa taagcatata aaagaagtat tcaccattgt tacatatcag 43440

gtaaatgcaa attaaaacca gaaacaagat aatagtacat tctgtgttga acagctaaaa 43500

ttagaaagtg ctaatcaggc atgtggaaca gttaggacct tcattcattg cttttaggat 43560

tgtaagtgga attgcttttg aaaaactgga atacctacct agacataaag tctatcctat 43620

gattcagtaa tttcattctt atattcaaag tatgtaacaa gagacataag ttcatagtct 43680

gccaaaacag caattaagag gcaaattaga cattgaaaaa agaagccaaa tatgtaactg 43740

tacatattgt atcttaacat ttacatgaag atcaaaaaca agcaaactta ataaattaca 43800

atcaaagcca aaattgtgga gcgacatatt ttaatttttt tttttttttt tttttttggc 43860

tgcactaagt cttagttgca gtatgcagga tctttagttg tggcatgtga gatctagctc 43920

cctgaccagg gatcaaaccc cagctccctg cactggcagg cagattatta gctactggac 43980

caccaggaaa gtccccagag gaattattga gaaagcagat aaatgaactt tctgaaatcc 44040

tggaaaagtt ctgtttgtca cagtatttag tgtaaaattt catcaagctg tacacttaag 44100

attagcacac tttggaccat gtagatgaca acaaccctga tccaaactgg agccaactct 44160

atgcagtcaa cagatgtgcc aaagggcctg taatatcagc aaaattacaa tgcaagaact 44220

tgccagaaaa atccaagaag ctttgtatgg ccatgatggg gaaatttacg gaggggtcta 44280

tggagtggct gcctctgaga taatgaaagt caagcacttt gtgttctgct ttgagccagt 44340

attgatcagt gagtccaata attaggcaaa aagctggaaa ctgaagcttg gcaggccccc 44400

atgcatctgt ctccctgtct cattgccccc aaactcagaa taatggtttc ttccctctgc 44460

cttccaattc tcacacaaac tcttacttta gacaactcaa acctggaact agataaagaa 44520

ctgactctgg gaagtgcagt cccagccacg tccaaagtac tacaacataa gacagcccaa 44580

ccaactttac ttgtagcttt cctgttgcaa ggtctctttt ataaaagttc aaaatactgg 44640

gtccaattcc cagtcataag caaggataaa gaacaaaata caaaaataac ataacagatg 44700

agactcttct atcaaggctt ttgcacttat tgatttccac tggattactt attagatagg 44760

gaaagctttt aatggtaaaa actaataatg tttttgtgtg aaaaaaaaaa aagagagaga 44820

gagagaggtg gggggcgggg aatagtgggt aggcaatttc cagtttccac catactagat 44880

caaaatgaaa ttaccattta ccaaagaaac tacaaatact tgaatgtcga taccacaatg 44940

actgcacact tagagaacaa agagaattaa ctggtagcta ataataataa taaaagatca 45000

ctcatgccta tatattaaaa aaatacttta aatgcctgat gggtccagaa aactctgtaa 45060

tagaaacaac atttacatct gaaccctaat aaaatcaccc cataataaaa cataagtggc 45120

tagagctgaa aggtattaga ggaaatttat caccttaaac attcatatca gaaaattaaa 45180

ccggaaatca atgatcaagc agtagcccac tttaaggatg aggcactaat ctttgctttg 45240

agtatgaatt ttcttagaac acaataaatg atggtcatgg aggtattatc acatgattta 45300

cataacagag tttctctcag ttataggttt ttccaaaaga tgggtgacaa tttcttacat 45360

tcatagggtg tcttcacagt gtgagatctc acatgctccc taagctttga aaaagcatta 45420

aagacattcc ctcattcctt actttatata ttttctctgg tgtgagtttt cacatgtctt 45480

cgaaagtaag caggacaaac aaaggctttc ccacattcct tacattcata gggcttctca 45540

ccagtgtggc ttcttacatg tcttcgaaag gatgaaggac aactgaaagc tttcccacat 45600

tccagacatt caaagggttt ctctccagtg tgcatccttg tatggacagt caggtatgaa 45660

gaatggcgaa aggctttccc acattcctta cattcatagg gtttctcccc agtgtgtgtt 45720

ctcatgtgga tcctaagggc tgaggggtaa atgaaggctt ttccacattt cttacattca 45780

tagggtttct ctccagtgtg agttcttata tgtaccgtca ggtgggatga gctgatgaag 45840

gctttcccac attccttaca ttcataaggc ttttctccac tgtgagttct taagtgttca 45900

gtgagggatg aggaacgact gaaggctttc ccacattcct tacattcata ctgcaccttt 45960

ccaatgtgat ctttcacgtg tgctcgaaag gacgaagggc aactgaaggc ttttccacat 46020

tctttacatt catacggttt ctctctactc tgatttttca catgtgtatt cagggaagtg 46080

ggaagataga aggctttccc acattctagg cactcatagg gtttttcccc tgtgtgtttt 46140

ctcatgtgga tgctcaagga ggatggacaa ttgtaggctt tcccacattc cttacattta 46200

tagggtttct cgcctgtatg tgttctaacg tgtacagtga gcttcgagga ctcactgaag 46260

gctttcccac actcttgaca ttcataaggc ttttctccag tatggattct tatatgtatg 46320

ataaggtgag aggaggaact gaaggccttc ccacactcct tacactcata tggcttatct 46380

ccactgtgaa ttcttttgtg tttggagagt gaggaggaac agctaaaggc tttcccacac 46440

tccttacatt cataaggctt atctccactg tgaattcttt tgtgttctgt gagggatgaa 46500

gaacagctaa aggttttccc acattccctg cattcgcaac ttgtctttcc tgcatgaatc 46560

ttcatatgtg cccgaaagaa ggaagaacaa ctgaaggctt tggtacattc cttacattca 46620

tagggtttct cttcagtggg agttttcata tgcttcttaa aacaagcaaa aaaatggaaa 46680

cctttcccac attcgttaca ctgatagggt ttgctaccag tgtgagacct aatgtgattc 46740

ttcagggata aatgatctat gaaggccttt ttacacacac ggcactcata ggctcttact 46800

ccggtagtac tcttgagtat attaaggtgt gaaatttggc tgaaggttag gccacactga 46860

ttttcttcat tatgttcgta gatgttctca accccatgaa ttctttcaaa aataaaaagc 46920

acattattag tggcaagtaa atttttacca ttttcagtgc ctatgtgtat ttttctggcc 46980

tgtccaatga taagatgaaa gtactcacat tgtgctctaa gatagacaat attatgactg 47040

agtttttgag ttatgagatt tttctgatgg ttgctcaatg atttatgtgt taaacatgga 47100

agggctcaag tctcacttgt gaaaaaaata tcttatgaaa agtctttagg aaattacaat 47160

ttttgactaa gtttagattt ttcttttttt taattctgtg atattttaag attctttcct 47220

gagacactac ttttctctta tatgaatggc acttacctca aacgtttctt gtggttttca 47280

ttctgataat caatatcatg gtattcccag attttatgta aaatggacag tgaggaatca 47340

ctccttttga atcctactac atcctgttca ttggatattt ttccataagt atcttgtgga 47400

gcaactgatt cattaatttt aagttgaatc tcgaaacctg aaacaaagta gcaaaggcac 47460

ccatgagcaa aggactttgg cagtacagct ggttcaggac tttggtaata agcacacggc 47520

cgaaatagtg aagtgattag gaataatccc atgaagatgg aaactgatgg tagcatagat 47580

aagatattac taggagacta aaacaaaaag aaatggaatg aaacacacat gaccaaatac 47640

tacattacga agacaaaatg tggtctttcc caagaacaag gaacaggtga gaggactgaa 47700

gaaaagtcaa aatagtaatc aaggacttcc ctggtggcac agcggataag aatccacttg 47760

ccaatgtagg ggacactggt tcaatccctg gtccaggaag attccacatg ccacagagca 47820

actaagccag tgtgcagtaa ctactaagcc cacaagctac aaatactaag cccaagcact 47880

gcaactagag ggcagtcctc actctctgca actagagaaa agcccatgca aagcaatgaa 47940

gatccagcac agtaaaaaat gaaatttttt taaataaata aaatagtaat ctaagatatc 48000

tgtggaagct cagcttcctc agacaaaaca aagcttcatt ttgatcttat gttatttttc 48060

ccaaatgtaa tatcccccaa ccacatcagg tattcttgct ttactgatgc tccccctgga 48120

cagacagtct tctctccact gtctctaggt cctcctcttg tttccactgt aagatcacat 48180

acggtttgtg tagtggatac cctgttcaca tagaaaggta tatcgtgagg gaaaacagtg 48240

agttaccaca tatatttcta tgaaacagag caaaaactgc tcttctaata ctctaaagca 48300

agtgataggg atagaatagg atagttatac aggataggat agttatgcag aagtcccagt 48360

agtggattag agataacgag ggaaacctag agaactttga gagaccagtg gaagttcagt 48420

tcagttcagt tcagttcagt cgctcagctg tgtccgactc tttgcaacct ttgaatcaca 48480

acacgccagg cctccctgtc catcaccaac tcctggagtt cactcaaact catgtccatc 48540

aagttggtga tgccatccag ccatctcatc ctctgtcatc cccttctcct cctgccccca 48600

atccctccca gcatcacagt cttttccaat gagtcaactc ttcacatgag gtggccaaaa 48660

tactggagct tcagctttag catcattctt tccaaagaaa tcccagggct gatctccttc 48720

ggaatggact ggttggatct ccttgcagtc caagggactc tcaagagtct tctccaacac 48780

cacagttcaa aagcatcaat tctttctttc actcagcttt ctttatagcc caactctcac 48840

gtccatacat gaccactgga aaaatcatag ccttgactag atggaccttt gttggcaaac 48900

taatatctct gcttttcaat atactatcta ggttggttat aatttccttc caaggagtaa 48960

gcatctttta atttcatggc tgcaatcacc atctgcagtg attttggagc caaaaaaaaa 49020

taaagtctga cactgtttcc actgtttccc catctatttc gcataaagtg atgggagcag 49080

atgccatgat cttcgttttc tgaatgttga gctttaagcc aaccttttca ctctcctctt 49140

tcactttcat caagaggctt tttagttcct cttcactttc tgccatacag gtggtatcat 49200

acaggtttga ccacacaggg tcagacattc ttgtgcctga tacaaaggag gaactagtga 49260

tgtaagcctg aaatcaactc aaagaaggca gtcctttccc cccacacaca ctttcctttg 49320

acaataaaat tatagcccac ttaatcctca gtgcagggct ccctcatcta tccacttaca 49380

tctcttgcaa gcatcttata ctaataaatc taccttttgc ctatcagttt gcttcttgct 49440

gttcctttcg cactgagata caaagactct gaccatcatt aagtccagaa accaggtatg 49500

tgatctcagt tggaagacca tgggttttgg ccaggctgaa gttgcagcct catgagttca 49560

agtcccaaac tggattttgg ccaagttcga atcctggcac atgagctcaa actctggcac 49620

acgggttcaa gtcccaatct gaagtgaact ttttcaccgg tgtgagttta actgcaacaa 49680

aatatctaat ttatctttta aggggatggg gcagtctgat gaatagaact gatctgatat 49740

tcacacatag acctcgatat tattagaagc tggtccagta ctcacaggca ggatatgttg 49800

ttctcctgtt gaagacaaat aatctccgaa taccaatctt aggcaaggtt actctaatac 49860

catgttaaaa tgaaacaaaa taaggctact tcatgattta gcctaggggc aaagtattac 49920

tagaatttcc cctgctttct gtcagcatcc aatctaaaat gaacacctgt gaactagggt 49980

gacagtggcc ctcaaagatt cctatctaaa tagaacaatg gaacagaata gagagtctag 50040

aaatagaccc acataaatat agtcaactta cctttgacaa aggttcaaag gcaatacaga 50100

aatacagcaa ttcagaaata cagtagagaa aaaaataagt ctttccaaca aatggtgcta 50160

aaacaactac tagacattaa ggtacatttt taaaaatatc ttgacataga cttgcaaaaa 50220

attaactcaa aattcacagg gaggcataca gggctttggg ataactgggg tccatcaata 50280

gagagtaaaa aattagctca aaatggatca caaatctaaa tgtaaaacgt aaaactatgg 50340

aattcctaag agacaacagg gaaaaaaagt agatgcccta agatctagtg atttgctaga 50400

tatgtgaaaa ataatcaagg aaaacttcac taaatggagt tgagaggcca gaagaggaag 50460

ttttcatcta attctttaca acattaatta cagggagaat tgtccatcag agaccccaac 50520

aagaatcaca aattataatc ctaacaggaa aagatgtaca tcacatctcc tatcagaaat 50580

cactactcca gcaactcagc catcatcacc atgaactctc acctttatcc agtggacttt 50640

catccaaaac aacctctctc aatttcctcc gttttctcta taaaattaca ttcttctcca 50700

ttgttccgtg gacttgccta tggcttttgt tatagctttc ttatctcaaa ttgcaattcc 50760

cttgctattc ccaagtaaac tatttttttg ttggtaaaat aactttttat ttttaaagcc 50820

aataagtaac ctgaaaagca caattcatga aagaaagaac tgataagttg gtcttcatta 50880

atattaaaaa tttttgctct gagtaaaaca ctgtcaagaa aatgaaaaga catgccacat 50940

acttggagaa aatatttgca aaagacatgt gtgataaaga actattatcc aaaatatata 51000

cagaagtctt aaaactcaac aataagaaaa taacttgatt ttttttaaat aggccaaagt 51060

aagaatatac aatggagaaa aagtctcttc agcaagtggt gttgggaaca ctagacagtt 51120

tcatgtgatc aaggaaacaa caacacaccc tcacatcata cacaaaacta tacttggcca 51180

gaagccagca cgggagatcc catccatgac aaggtcatgt gaaggagcct gacaagcaag 51240

gcttcaggac tggagggacc ccctggacct gctcgagcat ctaccccaaa accaaaatct 51300

tactatttat gcctttcacc aactcttttg acattaacag ggggttatcc ccgaccacct 51360

ttttctggaa aaagtcaact ttgggctcta gttgataaat ctcctgggca taaaaggagt 51420

atttcaattt aaaccccctg ttagcattct agcttacttg gcaggtttat ccagactctt 51480

gcaacaatgt tgagccaatg cttgctgcca aattcccaca tcccttatcc attgtgttcc 51540

tgggagtgca tacagttaag atggagaaaa aacaagtagt agccttagca ttagcaatat 51600

tagactttga gttaataagt tctttctttg ctgtaaccta ctgaatcttt gctccataaa 51660

aacgtaactc tgtactttaa gggtgacgcg ggctaaggaa tttaacaaaa aacatttcaa 51720

gggaaagtaa gtgttttagc tgaccaacct ttatcaaaaa ggggtcataa aatttcaaca 51780

ggcctctggg caagaagata atgtacaaaa aataagaccc ttgtttatga aatggtatgc 51840

aggaagaaac ctggtatcga taaaagttaa aactgatgga atgttgagct gactctgcat 51900

ttttcacttt gctcaatgta taactcaggg tataaaagtt ccctctgaaa acaaagtgac 51960

gggccttgct caccaaagct tggtctcccc gtgtcattct ttctctctct ttccctttcc 52020

ttttcaggtt gatcccctgg agcgcagggg ccctctgagt tcactttctt gcctgggctt 52080

ctaagaccca ctcgagaagg tgcccaaggt ggggcacctt cagctacttg agagggcgcc 52140

tgtggcttat gtgaacagag caagtcccgt gctggggctt tattggcttt ctgcgtaaac 52200

caaggaatat caacctcttt ctctcctcta ttttcaggac tggaaaaggt cagttttcat 52260

gccaattcca aagaaaggca atgcaaaaga atgttcaaac tactgcacaa ttgcactcat 52320

ctcacatgct agtaaagtca tgctcaaaat tctccaagcc aggcttcagc agtacatgaa 52380

ctgtgaactc cctgacgttc aagctggttt tagaaaaggc agaggaacca gagatcaaat 52440

tgccaacatc cgctggatca tggaaaaagc aagagagttc cagaaacaca tctatttctg 52500

ctttattgac tatgccaaag cctttgactg tgtggatcac aataaactgt ggaaaattct 52560

gagacagatg ggaacaagac cacctgacca gcctcttgag aaatctgtat gcaggccagg 52620

aagcaacagt tcgaactgga catggaacaa cagactggtt ccaaatagga aaaggagtac 52680

gtcaaggctg tatattgtca ccctgcttat ttaacttcta tgcagagtac atcatgagaa 52740

acgctggact ggaagaagca caagctggaa tcaagattgc caggagaaat ttcaataacc 52800

tcagatatgc agatgacacc actcttatgg cagaaagtga agaggagcta aaaagcctct 52860

tgatgaaagt gaaacaggag agtgaaaaag ttggcttaaa gctcaacatt cagaaaacga 52920

agatcatggc atccggtccc atcactttat gggaaataga tggggaaaca gcggaaacag 52980

tgtcagactt tatttttggg ggctccaaaa tcactgcaaa tggtgactgc agccatgaaa 53040

ttaaaagacg cttactcttt ggaagaaaag ttatgaccat cctaggcagt atattcaaaa 53100

gcagagacat tactttgcca actaagctcc atctagtcaa ggctatggtt tttccagtgg 53160

tcatgtatgg atgtgagagt tggactgtga agaaggctga gcgccgaaga attgatgctt 53220

ttaaactgtg gtgttggaca agactcttga gagtcccttg gactgcaagg agatccaacc 53280

agtccattct gaaggagatc agccctggga tttctttgga aggaatgatg ctaaagctga 53340

agctccagta ctttggccac ctcatgcgaa gagttgactc attggaaaag actgtgatgc 53400

tgggagggat tgggggcaag agaagaaggg gacgaccgag gatgagatgg ctggatggca 53460

tcacggactc gatggacgtg agtctgagtg aactccggga gttggtgatg gacagggatg 53520

cctggcgtgc tgcgattcat ggggtcacaa agagtcagac atgactgagc gactgaactg 53580

aactgaactg aactgaatat aaacttgaga tttaccttaa taatgatgtg tcaatatagg 53640

ttcatcaact gtaatgaata tactactctg gcagaggcag tgcatttatg ggaaatcttt 53700

gtacttgcca gttaattttg ctgtgagcat acaaactgct ctaaaaaaaa aatagtgtac 53760

tggaaaaaaa aaatccatgt ccactgagaa ttcagaacat tactttattt ggaaatagat 53820

atttgcagaa gtaatcaagt taacatgaag ttatactcga tttgggtaag ccctaaatgc 53880

aatggctcat gtccttacaa ggacagaaag atgtagagac aaaggacaaa ggccctatga 53940

agatggaggc agagattaga gtgatatggc taccaaccaa ggactgccag caacaaccaa 54000

aagctaggaa gaggcaagga ggggttttct cctgagcctt cagaaggatc atgacacctt 54060

gatttcagac tttcaatctc ttgaaattta gaagagtaaa tttcacactt ccttggcagt 54120

acaggtggta cagaagacgc ctgccaaggc aggagacaca ggttcgatcc ctggtgcagg 54180

aagattccac atgccacgga gcaattaagc ccatgcacca caactactga agccctcgca 54240

ccctacaact accaaagccc tcgcacctaa agcatgggct ccccaacaac agaacccacc 54300

actgtgagaa gcccatgcac cccaacaaag agtagcccca ctcactgcaa ctagagaaag 54360

cccaagtgca gcaaagacct agcacagcct aaataaataa aatagttgtc tagctacaga 54420

ataaaactga ggagtagagg tagaggaagt ttttttttaa agtaaaagaa taagtttctg 54480

ttgttcctaa ctacccagtt tgtggcactt tgttatgaaa ggcctaggaa aatagaacag 54540

cctgttgtca tggacctacc cctaaatcat tcaacctaag tatataaatc ctataatatt 54600

tactttctaa cacactgcat tgttccccat gacatgtgtt tctccctcac tgccccaagg 54660

aataaaccca acttgttaaa ctggtggtct ttgggtacag ggaattaata gcagtaaaat 54720

tctcaaaagg tctcaaacca ccaattctct ccctgtcccc caaagtgacc cctgaagcct 54780

gtgcccacac catacacact taataagcct ccttgaggat tctgaccaca aaaaaaaagg 54840

aaaaacaggt tcaatatata tgtttatttt ctttcttttc ctcatgttaa tttcatattt 54900

atcttctcca ttactgaagc gttttgaata caatgacatc ttattgtcta tagtatatat 54960

tcaaaagggt ttgatacaaa ttcatcctca aaaaaaaaat gcttaaacaa tgaaagaatg 55020

actgaataaa gaaaaataat gctttcagta ccctgagaga aagtgaatac tttacaaccc 55080

tgagaaaatg aaaactctgc atttagtgcc agtggcaaaa tgggtaagga aactttcact 55140

aattcttttg cttgttttat attggtgaaa tactttaaca atacacatgt ttattttcat 55200

ggtgaagcca ctagattcca ctagctcctt ggggtcagtg accatgtctg tcttatttaa 55260

caatttattc cagtttctca acccaaatcc tagaacaagg gcaatttata agataaacct 55320

gttttctaaa taacataaca ttggaggaat gttaccatcc ttacctaatg aggccaggtt 55380

ccgaaagttt tccagcatca catctctgta gaggtttctc tgagtgagat tcagcagagc 55440

ccactcctct tgggtaaagt ccacagccac atcctcaaag accactgtgt cctaaaacat 55500

tccacatact ttgttcagaa aggtaccaac tcccccagca tgtgcaggag ggtgaatgag 55560

gctgacagta ctggggaact gagaatcaac tcacaggaag ttcaaaagat tcttttctcc 55620

caaatatcta ccctaggatt cagtcatcag atctttctca ttctatctaa gcatcctgac 55680

tgatcaaatt ctgtctcata gatttaacaa caatttttaa acactaaatt tatctttcca 55740

ctgtttgatg gtgaccagtt ccactcttac aataatcaaa aacatccaga acatattgca 55800

gaaacgagag aaatgctcta aaaatgaaat aatttccaca ttgaaagaag caaaaatccc 55860

ttgttagagg gacttccctg gaggttcagg gcttaagact gtgctttcca acgcagggga 55920

tgtgggttca atccttggtt gagggcctaa aatcccacat gccttggggc caaaaaacca 55980

aaacagaaaa cagaaaaaac aactgtaaca aaacattata ctgtaaaata gaaaaactat 56040

tgtaacgaaa aaaaactatt taaaaaaaat atccttgtta cagaaacaag ttcacctgct 56100

gccttattcc ccaccacagc aatcagcaca acacaaaaca ctaatataaa atttggatga 56160

ggtttaaatc cccaagagac aaggaccttt tctttttctt ttttccctcc atacacctgg 56220

agcccagaag tcagtgagaa gcacaatatt caacccaggc ccacagtcag tcatattttc 56280

ttacactcta ggcccaggag ctgtaaatta tagaacatga actaaaatca gcctatcacc 56340

tgtttatatt aattaaagtt ttactggaac acaataaaac atttatttat acactgtata 56400

tggctgcttt cacactaaaa tggcagaact gatgggaaat ccctgtcagt ccagtggtta 56460

ggactccacg ttttcactgc tgagggcaca ggttcaattc ctggtcaggg aaataagatc 56520

ccacaagctg cccaaaacaa acaaacaaaa aacaataaaa tgccagaact gagtaaaata 56580

gaaactaagg cctaaaatgc ttactatgtg acccaatgta ggaataggtt gttgatctct 56640

gctctaaatt taggatataa ataacctgat ggaatttaaa tattcttttg gagaattatt 56700

agctcttact tacctgtatc acatttctct gtaactcagc agctattctc tcttcctcca 56760

tgttaccttc atgaagaaag acagagtatt gagatgttac agctgagaca ggagaaagaa 56820

gacatgagaa tctgggtgtc cccacagtct ccacaatcaa gactgggttc agcagcacat 56880

ctgttatgag tgatcattct cctcaaaaca cacacacaca cacgcaaagc ccctgcatac 56940

ccaagcaaac acacactgat cttgtgagaa aatggagcaa gaaaaaaaaa ataataatag 57000

taacttactt acaaaacaga gactcacaga catagagaag gacattatgg ttgccagggg 57060

gaaggacagg ggaaagggat aattagggag tttagaatgg acatgtacac actcctatat 57120

ttaaagtgga taaccaacaa gggcctactg tgtagcacat ggaactctgt tcaatgttat 57180

gtggaggcct ggatgggagg agagtttagg gaagaatgga tacatgtata tgtatggctg 57240

agtgtcttag ttgctcacct gaaactatca caacactgtt aattagctac atcccaatcc 57300

aaaataaaaa gttttttttc tttaaagttg gagcaatttt atctcctcca gagctagaaa 57360

cagtcctctc actcttgtag atcattaagg aaaaaaaaag gtttaagagt ccattttgta 57420

agtgagtcag ccggagtcta aaaaatttgg ctgtcaatct gcccatgata atgaaaacat 57480

taaagatata agagcatgcc ttcagtagta agaatataca ttccatctca aaggaaagtg 57540

tgacttgtac accagatctc caggggttcc agacccaata tttatttctg cactgcctgg 57600

ccagccaaca tgcattacta ctcaatctct aactatgaga tcttcctcct ccgagggctg 57660

caaggtctcc aaaacctggt ttttgagaaa cagcacaact gactgaattt tacatgcagg 57720

tggcagagat gacatctcat gttaaatttt gcctgtacag gagatatagt cacagcataa 57780

caagaacacc tgaaatgaag ttggcatatg tgtcatctct gacagtagca tccacagcac 57840

tagtgcatga atttcaaata gtgaaaacac agcagtctct ctcacttcag tggaccatat 57900

atgtattgta gagccaatag aaacttcagt cacttagaca tgtgctcagt gttatccaat 57960

tagaaagcta agggagggtg tgtatttacc ttcaaagcta aatgcccaaa gagtgtgttt 58020

ctgaacatat accttataat atagttgtca gtgaggcccc tacgccctaa cattactggg 58080

gtctagccta gaatgctgtt gggcacacta tggagagtaa acaaataaag tattcccttc 58140

aactgtttta tctttgagct ccttctcttt ttttattaac ttatttttaa ttggagggaa 58200

gacttttcag agtcccttgg acaggaagga gatcaaacca gtcaatccta aaggaaatca 58260

accctgaata ctcattggaa ggattgaggc tgaaccttcg atactttggc catctgatgc 58320

aaagaaccaa ctcattggaa aagactctga ggctgagaaa gattcagagc aagaggagaa 58380

ggggatgaca gaagatggat gtcagctgga tggcatcatt gactcaatgg acatgagttt 58440

gagcaaactc tgggagacag tgagggtcag ggaagcctgg catgctgcag ttggacacaa 58500

cttagcaact gaatgcttcc ctggtagctc agctggtaac gatctgcctg caatgcaggg 58560

gagacctggg ttcgatccct gggatgggaa gatgcgctgg agaaggaaat ggctacccac 58620

tccagtattc tggcctggag gattccatgg actgtacagt ccatggggtc acaaagagct 58680

ggacacgact gagcgacttt cactttcact agtgactgaa caacaaatgc tttacaatgt 58740

tatgttggtt tattctatat atcaatgtga atcagctacg aattacatat attccctccc 58800

tcttgagtct ccctctcacc catgtccatc ccaccctcta ggttgtaaca cagcagcaag 58860

ctgagctccc tgtgctatac agcactttct actatccgtt gtacacatgg tagcatatat 58920

atgtcaatgc tactctcgca attcatccca ccgtctcctt cctccactgt atccacaagt 58980

ccattctcta catctgcatc tctattcctg cccttgagct ccttctcttg actaagccct 59040

atgtcttcaa caaggatgct ccccttaagg tccctacctc ctgctccttc atctcatatt 59100

tatttaggta atatttgccc tgtccaacta tgtgcagtca taggagacac agcactaaac 59160

aaaacagaaa aaccctaaag tacttgagtc ttataattgt tttaaggagg accagtaaat 59220

taccaaaata ttatcaggtg atggtatatg ctatataaaa atatatcacg ttatagagat 59280

aaggagatac acgcacattt atctttagca agtcagaacc acttatgtga cagggtagaa 59340

gcaggaaatt aaaacaactg ttttaggatt tccctggtgg tccagtggtt aagaatctgc 59400

aggggacagg ggtttgatcc ctagtccaga aagattttac atgccacagg gcaactaagc 59460

tgtgcaccac aactactgag ccagcactct agggccctca taccgcaact actaagctca 59520

cacgctgcaa ctactgaagt ccacacacct agagtgtgtg ctctgcaata agaagccaac 59580

gccatgagaa gcccacgcac tgcaaagaag agtagccccc actcgctaca actaaagaaa 59640

acccatgctt ggcaatggag acccagtgca gccaaaaata actttttaag aataaataaa 59700

tagaacaact gtttcaaata acacaaaaaa tcaatggctg gtgtggcaca tacatttatg 59760

cacaactgac cctcaaacaa ttgcaggggt tggggtacca agcctccagc aatggaaaat 59820

ctgagtttaa ctcatagtca gccctcagta tccaaggatt caatcaatgg cccatcctgt 59880

atttaatagt gaaaaaaaaa aatctgcata taagtggaca catgcatttc aaacctatgt 59940

tgttcaaggg ttaattgtat ttataaatat catcagttcc tatagaagaa atattagtaa 60000

ttataggtta accattgatg tataatggca gaaaaagaac cacataaagg tgtaaatata 60060

tatatatgac aaaattaatg atattttttg gagaaggaaa tggaaaccca ctccagtact 60120

cttgcctgga gaatcccatg gacagaggag cctggcaggc tacagtccac agggtcgcaa 60180

gagtcagaca ctacttagtg actaaaccac cacccatgat atttttaaaa acttagaaaa 60240

actaaaatgg caatactacc caaagcaacc tacagattta ttgccatccc tatcaaatta 60300

cccatgacat ttttcacaga actagaatac taaaattttt atgaaatcat aaaagaccca 60360

gaattgccaa agcaatcctg aggaaaaaga atgaagcaaa aggcataacc atcccaggct 60420

tcagactact acagaactac agtaatcaaa acagtgtggt atttgcacac acacacacac 60480

acacacaaaa aaaaaaaaaa aacaggcaca cagatcaatg gaacagaata gagagcccag 60540

aaacaaatcc atactcctgt ggtcaattaa tcgtcaacaa aggaggcaag aatatacaat 60600

gggtaaaaga taatctcttc agcaagtggg tgctgggaaa gctggacagc tgcatgtaaa 60660

tcaaagaaat tagaacacac tctctcccat gggaaaaagg gaatcctact acctgttggt 60720

aggaatgtaa actggtacaa ccattgtgga gaacagtatg taaggttcct taaaaaacta 60780

aacacagaac taccgtatga tgcaaaaatc ccactcccgg gcatatatct ggagaaaagc 60840

aattcaaaaa gatacacgca tccctatgtt caaagcagca ctattcacca cagcacaaca 60900

gtgaagtcag gtaaacaacc taaatatcca tcagtggagg aatgaataaa gatgtgatac 60960

atatataaaa tggaacacta ctcagccatt taaaaaaaga aaggaataat gctatttgca 61020

gcaaaacggg tacatccaga aattatcaca ctaagtgaag taagccagaa agtgaaagac 61080

aaataccata tgatatcact tatatatgga atctaaaatg tggtgcgaac aagccaatct 61140

acaaaatgga aaaagactca acggatatag agaacagatt tgtggttacc aaaggggaga 61200

ggagacagga cagggatgga ctaggagtat ggaattagta gatgcaaact atagaatgga 61260

taaacaacaa agtcctattg catagcaatg ggaattatat tcaatatcct gggatagact 61320

ataatgaaaa agaatatact acataaaaaa agactaaata ttacatgtga taaatataat 61380

tcacatcaat agaaaaagat catatgactt atccccttca atagaaaata gtgacttatg 61440

tctatcaata cccattgatg ataataattt aacccactag gactagaagg aaataggctt 61500

taattagaaa atatatatat tatctacaaa cacctatagc aaacatccta tttaataatg 61560

aaatgacaga attccctctg ggatcaggaa caaggcaagg atgcaattta tatgcttttc 61620

tacattgaac tgaaagtcct gggaaatgca ctaaataaac aaaaatgtaa gacacaagga 61680

ataaaaaaga aaacgctaaa actggcatat gatcacactt ccggtgtgaa aatttttaaa 61740

aatctatgga taattgatca acattcagag cagcaactgg caaacagggg ttaaaaagat 61800

actaagtaat cagttcacta gtatgtacct gaatataatg tagcaaacta actcccacaa 61860

tcaaagaaaa agaaaaaaca ggttaaaatc tatctcttgc acctagaaag gagatgcaga 61920

cggagttaag caaacgtcac cccgcactca ccgaacaaac cgaaatccac agacaggcgc 61980

agaaacgagg tggaggctca ggtcctcgtg acccgaatgg caaggatgct tgtcctttcc 62040

gaaaggagag cagaggatgc ttttggaggc cgattaagca cgagaatggc ccttcaactc 62100

ttgcctgcag ccgattcacc gcgactgctg gaccctcaca gagttcctga ttcagtgagt 62160

ttgggaccag ccgcggctgc tacagacccc gggaggggcg gggagcccaa gcggcagcct 62220

tttgaccaca gatcccaagg ccgagttccc cacctcccga gcaccagact ttgggccgcc 62280

tgggtcgaac tggatgggaa aatttctatc agatttagac agagatgcaa gaagacaagg 62340

ccgcccgctc gcgcacgcgc agaagcgtgc tgctgcttcc ggtttccgag ccacgtacag 62400

cgtaaggggc ggggtctcgg gagctgggaa atttgggatg gcggttgcag cttccgccat 62460

tgcaagcgga gaagtgctag tttcctccag ggaaagacgc gtcggggtct tggagccggg 62520

taagccgagg acttggcgtt ggatgacgct ggagcaggac caggcctgtg gcggggcttg 62580

gttgactagg cctgtggggc ggagctggga cgcgaccagg cctaggggcg tggcttagtt 62640

gaatattccc atgtgggatc ttagtttccc aatcagggat tgaacctctg ttgcctgcat 62700

tgcaagacag aaattaacca catgcttaac cactggacca ccagagaagt cccgaggtgg 62760

taggtttttg ctgagcccgt gtataacttt cctccctgcc accgtggcta ctttgttcag 62820

gagcccactg gggagacgag cagctggaaa aggaggctta ctggtatcca cagaaccagt 62880

tattctattg tcatagtcac tctgcagata cagaatggaa cccccacccc aaactttatt 62940

tgcatgtcaa gatattatgc cacacacata caccaagagg gtgtgaatat gtttaaaact 63000

tacattacaa atggcccttg ggtcagcaag gtaaaataac tctgcttgtg agttgccatc 63060

aaacatccag ttttttaaga cacttgagac atattctcca ccattaattt ctctcagtct 63120

tccatggacc tttcttgaag caataaaaag ttctcaatgg tgggatttac caaccatatg 63180

tttccacctt ctctcccact ccgtcttttt ctcccaacct tgcctgtctc tctgctgctg 63240

ctgctaagtc acttcagtcg tgtccgactc tgtgcgaccc cagagatggc agcccaccag 63300

gctcccccgt ccctgggatt ctccaagcaa gaacactgga ctgggttgcc atttccttct 63360

ccaatgcatg aaagtgaaaa gtgaaagaga agtcgctcag tcgtgtccga ctatttgtga 63420

ccccatggac tgcagcctac caggctcccc cgtccatggg attttccagg caagagtact 63480

ggagtggggt gccattgcct tctccggcct gtctctctgg gaccgttaat actcaacaca 63540

tctttctcag ttcttcatcc ccactaatct atttagatgg gaacttttga ctcaatgaag 63600

agccactatt aggttcacac ctgaaagatt ttttttccct tttttaatct ctcaggctct 63660

gatgtcctaa tatactaatt tatgttggct tcccttaaag accctccact catgtttctc 63720

cgactcacta attagttcag ttttctgatt tacttttctc aataactgac ttctcatcta 63780

cagatgtggg ggaaagaaag attatagacc catatgaatc agtataaacc ccacaatgcc 63840

tctgcaattg cacagttctg gcaacccact ccagaattct tgcctgggaa atcgcatggt 63900

cagaagggcc tggtgggcta tataccatgg gatcgcaaaa gagtcagacg tgacttatca 63960

actaaacaac aacaatctca aagaactaga ggcactatga tctattgttg gagatttcat 64020

agtcaagagc ttactcattc cttgtactaa tctctgaaac actcctatcc tcttaaaaaa 64080

atataagggg aattattttg tggggaaaaa aaagggttat aaattctatc atgtgcccac 64140

atttcctgtg gccccccaac ccaactgcca ttctggcttc ttttcttctg aaatcagcat 64200

actttacagt agtaggtctc tgttcagctt tctctagtat tcctctacat cctgactcca 64260

aatacctttt cactttagta tgacaaaaca atacacctgg aagtatgatg tcacaaggct 64320

tcactaacat cccctcctac ttcttccagt ttctcaacca taatctccaa gcctttcaac 64380

ctccctattc ttctactttg agtctaggtg gtggtgttgt tgtccagttg ctcagtcctg 64440

tccaacccca tgaactgcag catgccaggc ttccctgtcc ttcaccagct cccagagctt 64500

ggtcaaactc atgcccattg agttggtaat gccatccaac catctcatcc tctgctgaac 64560

ttttatattc acctgatcta acctcccctc aagagaaata tttttatctc tggaaaactt 64620

tgttatctaa agatatacag tttctgggaa ttccctggtg atccagtggt tagggataag 64680

tgctttcatt gcagggggcc tggattcaat ccctggtcat ggaactaaga tcttgcaagc 64740

ctcccagcca aaaataaaga aataagacac aaagtttctg aggataaatt ataattaaat 64800

cagatttagt aggttaattg tatagttcat gatgtaacac tgagaaacaa gtcctcacac 64860

caaactgagt aacagctatt aaaaattccc tttcctgggg acttccccag tggtccagtg 64920

gttaagaatc tcccttccaa tgcaggggat gctggctcat ccctggtgag ggaactaaga 64980

tcacacatgc tgcagggcag ctaagcctgt gcgccaatac taagatccaa tgcagcaaag 65040

taaatacatc aatataaagt aaaataaatt tttaaaaatt ccgtttcctt ttgctgaaag 65100

acaacttagt tcagttcagt tcagttcagt tcatttgctc agttgtgtcc aactctttgc 65160

gaccccatga attgcagcat gccaggccac cctgtccatc accgacaact cctgaagtcc 65220

acccaaaccc atgtccatcg agtcagtgat gccatccaac catctcatcc tctgtcgtcc 65280

ccttctcctc ctgacttcaa tctttcccag catcagggtc ttttacagtg agtcagctct 65340

ttgcatcagg tggccaaaat attggagttt cagcttcaac atcagtcctt ccaatgaaca 65400

cccaggactg atgtccttta ggatggactg gttggatctc cttgcagttc aaggggctct 65460

caagagtctt ctccaacacc acagtttaaa agcatcaatt cttcggcgct cagctttctt 65520

atagtctcac tgtcgcatcc atacatgacc acagaaaaag ccatagcctt gactagacag 65580

acttttgttg gcaaagtaat gtctctgctt tttaatatgc tgtctagatt ggtcataact 65640

ttccttccaa agagtaagcg tcttttaatt tcatggctgc agtcaccatc tgcactgatt 65700

ttggagccca aaaaaataaa gtcagccact gtttccccat ctatttcccc atgaagtgat 65760

gggaccggat gccatgatct tcgttttctg aatgttgagc tttaagccaa ctttttcact 65820

ctcccctttt actttcatca agaggctttt tagttcctca tcactttctg ccataagggt 65880

ggtgtcatct gcatatctga ggttattgat atttctccca gtaatcttga ttccagcttg 65940

tgtttcttcc agcccagagt ttctcatgat gtactctgca tataagttaa ataagcaggg 66000

tgacaatata catccttgat gtactccttt tcctatttgg aaccagtctg ttgttccatg 66060

tccagttcta actgttgctt cctgacctgc atacaggttt ctcaagaggt agatcacgtg 66120

gtctggtatc cccatctctt tcagaatttt ccacagttta ttgtgatcca catagtcaaa 66180

ggctttggca tagtcgataa agcagaaata gatgtttttc tggaactctc ttgctttttc 66240

catgatccag cggatgttgg caatttgatc tctggttcct ctgccttttc taaatccagc 66300

ttgaacatct ggaagttcac ggtacacgta tttaggattc ctaaagctta ttgtgaactg 66360

tgcactgtcg ccttaattat ctgcctccat gatatgacta ttcaattatg cacccaaatt 66420

tgaaagcaat tgtcatttat gtaagatgca tgaactactg aggaaagcaa acctccgagc 66480

caatcttggg ccacagcccc gccatgcggg tccggatcct gcgcctcagt gcctctcagc 66540

ggaaggaaaa gggtataact cccggatccg ggactttaga agcagtggcc tctagggccc 66600

gcccaacctc cgaggcccca gcccttaggc tgcacgcggg cactggaagt gcgcctgcgc 66660

gtttgtattt gcgcgtgggc aacgcggaag gtctgttatt tgtgtgcccc ggctccggtg 66720

tgaggtgaaa ttttcgtttc tctcgtctcg tctctgtgtg gtcaagcagg aggggaacag 66780

acccaagagg aggcttgaag tccaaggaca gtggcctcga gggctgacct ggccgccctg 66840

gcttcccgcc ggtgctgcgg ggcggcggcc cctcctccga gagctcggac ccactcggcc 66900

cgcaggagtc ccggcggcag tcccgggagg aggcgggccg ggccggccta ctctccatcc 66960

gcgtggctcc cgatttcaca agcctccaga gcagcctttg ggtccttcct ccgcagggaa 67020

aagaaatagc atctctgtcc ttctcgactc cccgggtccc ggtgggctct cggtggcttt 67080

actccggccg tggttgtcga tcattgaagt ttggtaagtc atattccgtt ggaaagagaa 67140

gaaacctttg taggttatta gggagcacgt gtgtttgatc ttaataagag taggagtgaa 67200

ggcattcata taaataataa atttaccata tgagagcccc gtaaagcccc aaaccttcaa 67260

aatatagaag cctctgggaa ggaagccaat cgaggctgtt ttccgttagt agctgccgtt 67320

ggaattgaat ttacgtaaga tgagttttga cgggtagatt tttattctag gtattcatat 67380

agaatttctc tttctttttt tttcgcccgt gctgcacggc tggagggatc ttagttcccc 67440

caccaggagg tgaaccctgg cccacgggat tgacagtgcg gagtcctaac cactggacca 67500

ccaagaaatt ccctcatctt gaaattttta tcttaagaaa atttgtggtc aataaaagga 67560

atatccatga atctaagttc actaatttaa gattttgtct gtcattttat atatatataa 67620

aatgatgaac catttaatgg taaattgcag acattgactt ctaagaatga ggacagtttc 67680

ctactaagaa caaaactccg ttgtacatcc aagaaaatca aaatttctcc agttttccaa 67740

aagtatattt tatagttgtc tggttttgga gggagctgga ttcaaataag gtttgcacct 67800

tgcatttagg ttttacatct gttttaccga aaacatttgc cagtattttt ggttcctgca 67860

gtccaggaga tttatatgga aaaatgttgg cattcccata tggtgatgtt ttcctttaat 67920

ttcatgtaat tgtgtatctc ataaagtgga cattgagtct agggtgtgtg tgctgtcgtg 67980

tctgtgtctt ttgcgtcctc atggactgta gcccaccagg ctcctctgtc cataggattt 68040

tccaggcaag actactggag tgggttgcca ttccctcctc caggggatcc tcttgaccca 68100

ggggtcaaac ccaggtcacc tgcctttgca ggcaggttct ttaccactga gtcacctggt 68160

acagccagta gtatctaata caaatgtaat gtgatttaaa aaattttagt agtcaaatta 68220

ggagaaaata tacatgtgaa gttattttta ataatatgtc atagctaacc caatttaccc 68280

agttattaat atataactgt tggtgaaatt aatgaccaat gtagcttata gaaagttatt 68340

agtgagaaat tttacatttc ttttgtacca agttgttgga ctctgatatg tattttacac 68400

ctaagctcac ctcagtatgg atgctaaatt tttattagaa attcctgatc tgtatttagc 68460

tttcagaaaa ttgactgttg aaaaaaaata tttggggaaa cccagattgc tccaaacata 68520

ctgaaagatt tttttagtaa ctgaacagtt ttatttctaa aatttaatta aaagtgagta 68580

aaatgacaaa ttcagcccct cagtctcact agccatattt cgtactcaca atagccgcat 68640

agagcaagac aatgactact gcgttagact gcaggtttaa agacttgaaa atttcccagg 68700

tgatgtgtac ttcatgttct atcacctcag gaggcacata aatgcaagtc gtccactgtg 68760

tattaggagt gcaacgcgtt tagttaaggt acccatctct gtctcgcaca gtcacatctt 68820

tcttggtggt aatctgtagg tgataagctt cccaggtggc tcggtggtaa agaatctgtc 68880

cacagtgcag gaatgctggt ttgatccctt tgtcaggaag atcccctgga gaagaaaatg 68940

acaacccact ccaggattct tgtctgggaa atcccatgaa cagaagagcc tgatgggcta 69000

atctgtgggg tcacaaaaga gttggacatg actcagcaac taaaccacaa caatctgagg 69060

gtgataactt ggcaacaggg gagtaatctg ttgcccagcc ctttcaccca gtggttttag 69120

catcacttgt tgctctttac ttctacctat ttttacactg gggtcttgaa aagtgcttgt 69180

tgttgttgtt gtttctaatt ctctttcttt ttttccaaat ttttccattc tcttgctgtc 69240

agtcttttgt aaacaagagc tttctctccc cacgtaccct gccttctcta tttgtttttt 69300

ccctttcacg gtttcacaac atattcccct gtttctcttt catttatcat attataaaca 69360

gatgctgtta tttgatgctc aggctctccc aaacttggtc aagagtagtc cttttaattt 69420

gattcatgtg tcctttcaac acatccctat aagtctttga gcgtcaattc ccaggtcact 69480

ttgaacttga ctgttgctgc ttagtcactt gagtcatatc caactctgtg tgaccctatg 69540

gactgtagcc tgccaggctc ctctgtccat ggaattctgc aggcaagagt actggagtgg 69600

gttgccatgc cctcctccag ggtatcttcc caacccaggg atcaaaccca ggtctcctgg 69660

ctgcattgca ggcagattct ttactgctga ggcaccagag aagccccaaa cttgactgtt 69720

aagagaccat aatcagtgat ttctccaaga agcctgtttc cttgtagaca ccaaggtctg 69780

tatgatacat ggattcagtg ttcctggggt ttaattgtgt ctaggccttt cagtggatat 69840

ctaaaatcga tattttcagg acttttctgg tggtccagtg cttaagacgc catgctgcca 69900

ctgcaggggg cacaagttcg atccctggtt ggggagctaa gatcctacat gccacacaaa 69960

gtggccaaaa atgtaaataa gtaagttttt taaaaaatat ttttaaaaaa cttacttatt 70020

tacattggat tcgtgtcaat gatggcaaaa ccaatacagt attgtaaagt aaaataaagt 70080

aaaaataaaa attgttttta aataaataaa taggcagtcc aagagaaaaa gaaagaagaa 70140

aagaaaaaaa atatttataa ctttatatcc tcctttccat ataaattata ttgtgaacgt 70200

actcttatat tattattctt caaaaaccca attaaaatat atcttttctt acatcatatg 70260

ttcatactca tattcctagt aaaaattttg ggcaacccag acctgctaag ttaatcgttt 70320

cctgcacact atttcgtgat cactgtccag tcatattgga gaattaacaa taatcttagg 70380

agttcaggct tttgagacag agtaacctcg aatcaggctc tggctcacca cttcctaagc 70440

ggtgatcttg gaccaattac tactgctgtt tgcgcatgaa ttgcctcaac tattgaatga 70500

cagtaagaat aacttgataa tcatatgagc tttcagtgtt tccagtacat aatgggtcct 70560

cagtaaatga caattattta tattcatgtc gtctacagtt tctaatgact ttcacataat 70620

atataatggc cgtcttctac agagcgatga ctaataaatg atgcttgatg acaaatcagt 70680

aatgcctact gcttaaagat tgatggcatg aggcaagact cccactacta cttaatcgga 70740

attgaactgt cttgaagaga cttcgggaat aacatgtata aggcagattt cactgtcaga 70800

ttcacagatg ccagcccttg attttttccc ttgtggcaag tacttgctga tgctcctttc 70860

agagacagtt ctttactggc ctgtgagcag aatgtctttg tggtctgggt ctacatagtc 70920

ctgcttctgg gctgtctagg agggtgctta agtatataac aatatcctgt tgtgtaaatc 70980

ctgggacttg gggagagaag ggtaagtctg cccatttttg tgagagaagt tctgcttaat 71040

tacatgatga attatcactg ttagccaaaa aaaaaataaa aaataaacaa ttttacctgt 71100

ctagacttta tctgtaaaat gaaattacat tattaaactt tttttccttt ttagcaaaat 71160

ccatttctaa tttcaggtga ggcagattgt agggcatttt atggcagcag tgtgtatgtg 71220

tttacttaga attagtgtgg ggtgtttcca ggcttgtgag tatggaaact gtgggcagcc 71280

tgaattctca ggttttgttt cttctcctta aggctttctc gggaattttt ctgtctttat 71340

gaagagaagt cagaagggga aaggactctg actggatgtc tgacaaatta ttttcaagtg 71400

agtaggaaat ttattctatt cttttagaaa agcacgtttc ttccttccag gtagatgtgt 71460

ttccttctag tgaaacagag ttttttattc caacagaacc tgcttaggga ctgagtccaa 71520

gttaaatttc ctcagatctt agggcctttc atttacataa gagaatgata ccatcatccc 71580

agtatttact aattcaatcc gatcatccat gctggttctg tgccctcatt ttgctataat 71640

agacagagag ctgacatgtg attctgagaa tttgtgctgt cactggagga gtgatgcatt 71700

tccagcattt attgtctata atgtgctcag aactattgct aatcctatca ctagagaaaa 71760

ttaagaccag aagaggtcac aatctagttg tggtgagata catcgttagg agagagagag 71820

gtactcttaa gctacacaat tggatgagat cactatgaat atgtccaggt agaggggaga 71880

aggggcttga ggactatcaa gccccagggt tgatggtggg agaacacgaa acctgtctag 71940

gctttgggtc tcccttcctc tttttcaagt gcacaactga tggagatggc aactgcctga 72000

tgccaggatg tttgtgatgg tgtaggatct cgtgaccttt gaggtcacag actttacagt 72060

acaatggctt cctcttgaaa aatgaagatc tgtccgttgt actgacttta ttttttctag 72120

gataaaaggc ttaggactta attgtgtata taagcttgag cttgggtttt aggcaaaaga 72180

tagggagtct attttgtgag agagagagag agagaatgtg aatatgtgtg cgttttagtt 72240

ttattttgaa gtttactggt ttgataaagt tgacatgaac attttgttgt ttctcaagtt 72300

atacctgaga gtatttggta tccctgaaga gcagactctc ggccccttat ttatggaagt 72360

atttgtcatc ccttacatgt tagtcttata tcaggcatct tcctctcaga gcagggcacc 72420

agttatgcag acctcgtctg atctttcagg tagaactaag aggaaagctg aagaaaatgg 72480

aaagttttcc aagagggggg aaaaggctaa ttatttattt atttggctgt gctgggtctt 72540

cattgctgtt cgcagctttc tctagttgtg acaagtgggg actgctcctt gttgtggcgc 72600

acaggcttct cattttggta gcctctcttg ttgtagagca caggctctag gctttcgggc 72660

tcagcagttg tggcacgtgg gctaagtttt tccacagcaa gtgggatctt ctcggaccag 72720

ggaccgaacc cacatcccct gcattggcag gtggattctt atctactgta ccaccaggga 72780

agtccaaact tttcttctct ttaacttttc cctcaccttt ggaaactttc tttcggcctg 72840

acagtagaat ctgtcccatt tttgaatttt aattttgtgt ttaagtcagt gtttgaaagt 72900

aaatacacat tggccttcat ttttaacacc ttccttccat ttattttcca ataacataat 72960

ttttggacat aacataatct gtaataacat aatctgtaat cttatttcag attgggagat 73020

ttgtctcaat accagaaggt cagcacctca gtggaatatt ttgaatggga aaaaatccaa 73080

cgtggtagac atg 73093

<210> 2

<211> 21

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 2

ggtcagcacc tcagtggaat a 21

<210> 3

<211> 21

<212> DNA

<213> Artificial Sequence (Artificial Sequence)

<400> 3

ggtcagcacc tcagtggaat a 21

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