1. A Serratia (Serratia sp.) HGS-487 strain for transforming ginsenoside Rf into ginsenoside Rg1, the strain having a deposit number of: CCTCC NO: m20191057, and the 16S rDNA sequence of the strain is shown in SEQ ID NO. 1.

2. Use of the serratia HGS-487 strain of claim 1, wherein: used for preparing the ginsenoside Rg1 by fermentation and transformation.

Background

Ginseng (Panax ginseng) is a traditional Chinese medicinal material in China. Ginsenoside (Ginsenoside) is the main active medicinal component of ginseng, has a plurality of physiological and pharmacological activities of resisting tumor, resisting oxidation, protecting cardiovascular system and nervous system, and the like, and is widely applied to the development of medicaments and health care products.

The ginsenoside Rg1 has good effect of protecting central nervous system, can delay and reduce apoptosis of neuron, has effect of promoting cortical neural stem cell proliferation and glia directional differentiation, can also reduce beta amyloid content in brain by improving enkephalinase expression, and has good effect of resisting Alzheimer disease. Rg1 also has good effect of protecting cardiovascular system, and can obviously reduce the incidence of arrhythmia after ischemia/reperfusion by regulating and controlling the endogenous mitochondrial apoptosis pathway; the blood flow of cardiac muscle can be reduced and the structural change of cardiac muscle can be inhibited by down regulating the activity of a RhoA signal channel, so that the cardiac muscle can be protected from being damaged; can also inhibit inflammatory reaction to change myocardial pathological change, thereby playing the role of resisting myocardial ischemia reperfusion injury.

Ginsenoside Rg1 is low in ginseng, and ginsenoside Rf is high. The conversion of ginsenoside Rf into ginsenoside Rg1 with higher medicinal value is one of effective ways to improve the utilization value of ginsenoside and obtain better components of medicine and health product.

Methods for transforming ginsenoside Rf into Rg1 include physical method, chemical method, and microbial transformation. Physical and chemical methods are easy to destroy the aglycone structure in the conversion process, and have low conversion rate, violent reaction and more byproducts. The microbial conversion method has the advantages of high conversion efficiency, strong specificity, low energy consumption, no pollution and the like.

Disclosure of Invention

The invention solves the problems in the background art, and provides a Serratia HGS-487 strain for converting ginsenoside Rf into ginsenoside Rg1, wherein the strain can convert ginsenoside Rf into ginsenoside Rg1 and has high conversion rate.

The inventor selects a novel strain, which is named as HGS-487 and is a Serratia sp strain, and the strain is deposited in China center for type culture Collection with the address: china, wuhan university; the zip code 430072 has a preservation date of 2019, 12 months and 17 days, and has a preservation number of: CCTCC NO: m20191057; the 16S rDNA sequence of the strain is shown in SEQ ID NO. 1.

The Serratia HGS-487 strain can be used for preparing ginsenoside Rg1 by fermentation and transformation.

Compared with the prior art, the invention has the following advantages: the Serratia HGS-487 strain provided by the invention can convert ginsenoside Rf into ginsenoside Rg 1; the strain is low in cost, quick in reaction and high in conversion rate when used for converting ginsenoside Rg1, and the conversion efficiency of converting ginsenoside Rf fermented for 12 hours into ginsenoside Rg1 is up to 67%. In the reaction for producing the ginsenoside Rg1 by transforming the strain, the target product is clear, the interference of other impurities is small, the ginsenoside Rg1 is easier to prepare, and the strain has obvious advantages in the process of producing Rg1 by mass fermentation.

Drawings

FIG. 1 is an HPLC detection chart of ginsenoside Rf and ginsenoside Rg1 in a fermentation liquid before fermentation and transformation of Serratia HGS-487 strain;

FIG. 2 is an HPLC detection chart of ginsenoside Rf and ginsenoside Rg1 in fermentation broth after fermentation and transformation of Serratia HGS-487 strain;

FIG. 3 is an LC-MS single-channel directional detection diagram of ginsenoside Rf and ginsenoside Rg1 in fermentation broth after fermentation and transformation of Serratia HGS-487 strain.

Detailed Description

The present invention will be described in detail with reference to specific examples, but the scope of the present invention is not limited to the examples.

Isolation and culture of the Strain

In the embodiment, 0.5g of root tissue of 5-year-old fresh wild ginseng collected from Changbai mountain is surface-sterilized with 75% ethanol for 20min, washed with sterile water for 3-5 times, put into a mortar sterilized in advance for standby use, added with 5mL of sterile water, ground into a suspension, uniformly coated on an LA culture medium plate, inversely cultured in a 37 ℃ constant temperature incubator for 3-5 days, picked with a toothpick, transferred to a new LA plate, and repeatedly purified until a single colony is obtained.

The monoclonal bacteria are picked into a 1.5mL EP tube filled with 600 mu L LB liquid medium, shake-cultured for 1d at 37 ℃ and 190r/min, added with 50% glycerol with the same volume, mixed evenly and stored in an ultra-low temperature refrigerator at minus 80 ℃, and each strain is stored at least 3 parts.

Screening of strains

Collecting the preserved strainInoculating 10 μ L of the extract into 10mL LB liquid medium, performing activation culture at 37 deg.C for 24 hr by shaking at 180r/min, transferring to 100mL LB liquid medium (liquid loading 100mL/250mL) according to 5% transfer amount, fermenting, and shaking at 37 deg.C for 180r/min to OD₆₀₀When the concentration is 0.8, ginsenoside Rf is added to a final concentration of 30 mg/L. After 12h fermentation culture, collecting fermentation liquor.

And (3) carrying out HPLC detection on each strain fermentation liquor sample, comparing the HPLC spectrograms and data of the standard ginsenoside Rf and Rg1, and determining whether the strains can directionally convert Rf into Rg 1. And calculating the transformation efficiency of the target strain to Rf according to the standard curve of the corresponding ginsenoside.

The ginseng endophyte capable of converting the ginsenoside Rf into the ginsenoside Rg1 is obtained by screening after the detection, and is named as HGS-487 strain. The conversion efficiency of the ginsenoside Rf fermented for 12h by the strain to the ginsenoside Rg1 is 67%, and the HPLC detection of the fermentation liquid before fermentation conversion is shown in figure 1; HPLC detection of fermentation broth after fermentation conversion is shown in FIG. 2.

The applicant adopts LC-MS single-channel directional detection on the fermentation liquor after the fermentation and conversion of the HGS-487 strain, and the detection result is shown in figure 3, so that the HGS-487 strain is further determined to be capable of converting ginsenoside Rf into ginsenoside Rg 1.

Sequencing, sequence alignment and analysis

The 16S rDNA sequence of the HGS-487 strain is sequenced by a biotechnology limited company, and the 16S rDNA sequence of the strain is shown as SEQ ID NO. 1. And (3) performing Blast similarity analysis on the sequencing result in a GenBank nucleic acid database, and determining that the bacteria are Serratia sp bacteria through Blast sequence comparison and evolutionary tree analysis.

Preservation of the strains:

the strain is preserved in China center for type culture Collection with the address: china, wuhan university; the zip code 430072 has a preservation date of 2019, 12 months and 17 days, and has a preservation number of: CCTCC NO: m20191057.

Sequence listing

<110> Shanxi university of traditional Chinese medicine

<120> Serratia HGS-487 strain for converting ginsenoside Rf into ginsenoside Rg1 and application

<160> 1

<170> SIPOSequenceListing 1.0

<210> 1

<211> 1449

<212> DNA

<213> Serratia sp

<400> 1

gctgacgtca gactacacat gcagtcgagc ggtagcacag gagagcttgc tctctgggtg 60

acgagcggcg gacgggtgag taatgtctgg gaaactgcct gatggagggg gataactact 120

ggaaacggta gctaataccg cataacgtct acggaccaaa gtgggggacc ttcgggcctc 180

acgccatcag atgtgcccag atgggattag ctagtaggtg gggtaatggc tcacctaggc 240

gacgatccct agctggtctg agaggatgac cagccacact ggaactgaga cacggtccag 300

actcctacgg gaggcagcag tggggaatat tgcacaatgg gcgcaagcct gatgcagcca 360

tgccgcgtgt gtgaagaagg ccttagggtt gtaaagcact ttcagcgagg aggaagggtt 420

cagtgttaat agcactgtac attgacgtta ctcgcagaag aagcaccggc taactccgtg 480

ccagcagccg cggtaatacg gagggtgcaa gcgttaatcg gaattactgg gcgtaaagcg 540

cacgcaggcg gtttgttaag tcagatgtga aatccccgcg cttaacgtgg gaactgcatt 600

tgaaactggc aagctagagt cttgtagagg ggggtagaat tccaggtgta gcggtgaaat 660

gcgtagagat ctggaggaat accggtggcg aaggcggccc cctggacaaa gactgacgct 720

caggtgcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgctgtaaac 780

gatgtcgatt tggaggttgt gcccttgagg cgtggcttcc ggagctaacg cgttaaatcg 840

accgcctggg gagtacggcc gcaaggttaa aactcaaatg aattgacggg ggcccgcaca 900

agcggtggag catgtggttt aattcgatgc aacgcgaaga accttaccta ctcttgacat 960

ccagagaact ttccagagat ggattggtgc cttcgggaac tctgagacag gtgctgcatg 1020

gctgtcgtca gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc gcaaccctta 1080

tcctttgttg ccagcgattc ggtcgggaac tcaaaggaga ctgccggtga taaaccggag 1140

gaaggtgggg atgacgtcaa gtcatcatgg cccttacgag tagggctaca cacgtgctac 1200

aatggcgtat acaaagagaa gcgaactcgc gagagcaagc ggacctcata aagtacgtcg 1260

tagtccggat tggagtctgc aactcgactc catgaagtcg gaatcgctag taatcgtaga 1320

tcagaatgct acggtgaata cgttcccggg ccttgtacac accgcccgtc acaccatggg 1380

agtgggttgc aaaagaagta ggtagcttaa ccttcgggag ggcgctacca cttgtgatga 1440

gcatcaaac 1449