1. The nicotine with low content of the hypoalkaloids is characterized in that the nicotine with low content of the hypoalkaloids is obtained by extracting twigs newly issued after flue-cured tobacco leaves are picked and cured as raw materials, and comprises the steps of raw material obtaining, extraction, purification and post-treatment.

2. The nicotine with low content of the hypoalkaloids according to claim 1, wherein the nicotine with low content of the hypoalkaloids is obtained by extracting twigs newly released after flue-cured tobacco leaves are picked and cured as raw materials, and comprises the steps of raw material obtaining, extraction, purification and post-treatment, and specifically comprises:

A. raw material acquisition: after the tobacco plant picks up the flue-cured tobacco leaves, fertilizing, spraying a plant growth regulator, culturing for 25-30 days until the length of the twigs is more than 60cm, and picking and drying when 40-60% of the twigs enter the bud stage to obtain a raw material a;

B. extraction:

1) crushing the raw material a, sieving the crushed raw material a with a 15-30-mesh sieve, adding a potassium hydroxide solution which is 2-5 times of the weight of the raw material a and has a mass percentage concentration of 3-5%, performing ultrasonic extraction at the temperature of 70-80 ℃ for 30-50 min, and performing filter pressing to obtain filter residue b and an extract c; adding a potassium hydroxide solution with the mass percentage concentration of 3-5% and the weight of 1-2 times that of the filter residue b into the filter residue b, ultrasonically washing the filter residue b at room temperature for 10-15 times, and performing filter pressing to obtain a washing liquid d and washing residue j; mixing the extracting solution c and the washing solution d to obtain a material e;

2) adding methyl acetate of 1/4-1/10 weight of the material e into the material e, fully stirring for extraction, and standing for layering to obtain a methyl acetate phase f and an extraction residual liquid g; adding methyl acetate with the weight of 1/10 in g of raffinate into g of raffinate, fully stirring and extracting to obtain a methyl acetate phase h and raffinate k, combining the methyl acetate phase f and the methyl acetate phase h, and recovering a solvent from methyl acetate to obtain crude nicotine i;

C. and (3) purification: carrying out molecular distillation on the crude nicotine i under the protection of nitrogen, and collecting distillation components at 100-120 ℃ to obtain a target pure nicotine;

D. and (3) post-treatment: and returning the waste washing slag j and the raffinate k generated in the extraction step to the tobacco field as fertilizers.

3. The nicotine with low content of hypoalkaloids according to claim 2, wherein the fertilization in step A is carried out by applying a three-element compound fertilizer containing 10-20 parts by mass of nitrogen, phosphorus and potassium and 10-20 parts by mass of urea.

4. The nicotine with low content of the secondary alkaloid according to claim 3, wherein the application amount of the three-element compound fertilizer is 15-20 kg/mu.

5. A nicotine with low content of sub-alkaloids according to claim 3, in which urea is applied in an amount of 5-10 kg/acre.

6. A low sub-alkaloid nicotine according to claim 2, wherein the plant growth regulator used in step a comprises the following components: 0.3-0.5% of compound sodium nitrophenolate, 0.1-0.2% of sodium naphthalene acetate, 0.001-0.002% of brassinolide, 0.05-0.1% of gibberellin, 2-5% of monopotassium phosphate, 2-5% of ammonium nitrate and the balance of water.

7. Use of nicotine low in a hypoalkaloid according to any of claims 1 to 6, in the manufacture of buccal tobacco, e-cigarettes and heat-not-burn tobacco with low levels of nitrosamines.

Background

Nicotine, also known as nicotine, is a pyridine-type alkaloid, is present in tobacco commercial crops, and is the most marked chemical component in tobacco. The tobacco product has high nicotine content, strong acrimony, pungent taste and low nicotine content, and is light and tasteless when being sucked. Nicotine has many uses and it can be used as an insecticide. The nicotine sulfate obtained by reacting nicotine with sulfuric acid is a widely used pesticide in the world. Scientific workers also research and develop various types of nicotine pesticides according to the structure of nicotine, are widely used for preventing and treating plant diseases and insect pests, and have the advantages of no residue and no public nuisance and high-efficiency pest killing. In addition, the use of nicotine in medicine is also increasingly recognized. Studies have found that non-smokers have a much higher prevalence in patients with alzheimer's disease and parkinson's syndrome than smokers, suggesting that nicotine has the potential to be a drug for certain diseases.

In recent years, with the rapid rise of novel tobacco products such as low-temperature cigarettes, cigarettes without mouth and electronic cigarettes, the demand of the novel tobacco products on nicotine is increasing day by day, so that the price of the nicotine is continuously increased, and the method for preparing high-quality nicotine at low cost has a very wide market prospect. Nicotine can also be synthesized artificially. The usual synthetic route is: methyl nicotinate and N-butenyl pyrrolidone are used as raw materials to prepare N-butenyl-3-benzoyl-1-pyrrolidone through condensation reaction, and (R, S-) nicotine can be obtained through reduction reaction. However, the artificial synthesis method is high in cost, and extraction from tobacco waste species is still the main way to prepare commercial nicotine.

The raw material for extracting nicotine is waste (including tobacco leaf review and powder in cigarette processing) in cigarette production process, and the extraction method comprises steam distillation, organic solvent extraction, subcritical extraction, ion resin exchange, etc. However, the waste in the cigarette production process is subject to monopoly control, and the raw material source is difficult and very limited. In order to overcome the above technical problems, it is necessary to develop a method for preparing tobacco alkaloids which can solve the above technical problems.

Disclosure of Invention

It is a first object of the present invention to provide a nicotine having a low content of a secondary alkaloid; a second object is to provide a nicotine use with a low content of said secondary alkaloids.

The first purpose of the invention is realized by that the nicotine with low content of the hypoalkaloids is obtained by extracting the branch newly generated after the flue-cured tobacco leaves are picked and cured as the raw material, and comprises the steps of raw material obtaining, extraction, purification and post-treatment.

The raw material obtaining operation is as follows:

A. and (4) fertilizing tobacco plants after tobacco leaf curing. And planting the flue-cured tobacco according to a normal planting rule, and harvesting leaves for baking. The leaves of the tobacco plant are not picked completely (3-4 leaves on the upper part of each tobacco plant are reserved). After the tobacco leaves are harvested, 15-20 kg of ternary element compound fertilizer (N: P: K is 15:15: 15) and 5-10 kg of urea are applied to each mu. The fertilizer can be uniformly sprinkled in the tobacco ditch, provides sufficient nutrients for the full development of twigs, provides sufficient nitrogen sources and promotes the synthesis of tobacco alkaloids.

B. The preparation and application of the plant growth regulator. Preparing a plant growth regulator, which comprises the following components in percentage by weight: 0.3 to 0.5 percent of compound sodium nitrophenolate, 0.1 to 0.2 percent of sodium naphthaleneacetate, 0.001 to 0.002 percent of brassinolide, 0.05 to 0.1 percent of gibberellin, 2 to 5 percent of monopotassium phosphate, 2 to 5 percent of ammonium nitrate and the balance of water; and fully and uniformly mixing according to the proportion to obtain the plant growth regulator. The plant growth regulator is diluted by 10-20 times with water again, and is sprayed on tobacco plants, 10-20 kg of diluent is sprayed per mu, and the quick growth of branches of the tobacco plants is promoted.

In the above growth regulator formulation: the compound sodium nitrophenolate and sodium naphthylacetate cell division and activation agent can increase the fluidity of cell sap and promote cell division, thereby promoting the quick growth of twigs; brassinolide and gibberellin are antidotes, and in order to enable leaves to accumulate more nutrition in the flue-cured tobacco cultivation process, a bud inhibitor is required to be used for inhibiting the growth of lateral buds, and the brassinolide and the gibberellin have a good detoxification effect on the bud inhibitor and can promote the growth of the lateral buds; potassium dihydrogen phosphate and ammonium nitrate are nutritional ingredients required by plant growth, and the tobacco plant nutrient absorption can be accelerated by spraying the tobacco plant, so that the growth of new leaves is promoted.

C. And (4) collecting the branches. After the harvested and roasted tobacco plants are fertilized and sprayed with the plant growth regulator, a large number of branches grow rapidly on the tobacco stems for 25-30 days, the length of the branches can reach more than 60cm, and about 50 percent of the branches enter buds to be harvested. Collecting the branch from the tobacco stem, spreading in the field, and drying in the sun, so as to obtain the product for extracting nicotine. The branch yield (dry weight) of each mu of tobacco field can reach 140-170 kg. The nitrogen fertilizer has enough trial amount, can promote the nicotine synthesis of tobacco plants, and the total alkali content of the plants in the twigs can reach 2.8-3.6 percent and is higher than the average value of normal tobacco leaves.

The extraction, purification and post-treatment steps are specifically operated as follows:

A. and (4) extracting tobacco alkaloid. The branches dried in the field are crushed into 15-30 meshes, placed in an ultrasonic extraction tank, then added with 3-5% of potassium hydroxide solution which is 2-5 times of the weight of the branch powder, and ultrasonically extracted for 30-50 min at 70-80 ℃. After extraction, the extract is filtered by pressure. Adding 3-5% potassium hydroxide solution 1-2 times the weight of the branch into the extraction tank, ultrasonically filtering at room temperature for 10-15 min to wash the branch powder, and press-filtering to obtain washing solution. Mixing the extractive solution and the washing solution for extracting nicotine.

B. And (4) extracting tobacco alkaloid. Putting the extract liquid into a liquid separating device, adding methyl acetate with the weight of 1/4-1/10 of the extract liquid, fully stirring for extraction, then standing for layering, and separating out a methyl acetate phase; adding 1/10 methyl acetate into the raffinate, and extracting once again; and combining the methyl acetate phases extracted twice, and evaporating the methyl acetate to dryness to obtain the crude nicotine with the total plant alkali content of more than 90 percent. The methyl acetate recovered by distillation can be used for the extraction of nicotine of the next batch again. And (4) supplementing potassium hydroxide to the raffinate after the two extractions, and returning the raffinate for extracting the sample again.

C. And (4) purifying the tobacco alkaloid. And (3) carrying out molecular distillation on the obtained crude nicotine under the protection of nitrogen, and collecting distillation components at 100-120 ℃ to obtain pure nicotine with the total alkali content of more than 96% in plants. The pure nicotine is measured by gas chromatography-mass spectrometry, the content of nicotine exceeds 96%, and the proportion of other tobacco secondary alkaloids does not exceed 4%. Compared with the nicotine (the content of the hypoalkaloid in the tobacco is more than 7%) extracted from the crushed tobacco in the traditional method, the proportion of the nicotine in the obtained nicotine is greatly improved, and the quality of the nicotine is good. The method adopts the liquid chromatography-tandem mass spectrometry to determine the tobacco-specific nitrosamine in the nicotine, and compared with the nicotine extracted from the tobacco processing crushed tobacco by the traditional method, the method has the advantages that the tobacco-specific nitrosamine precursor compound (tobacco sub-alkaloid) is greatly reduced, and the tobacco-specific nitrosamine is reduced by over 40 percent. The application in the novel tobacco products is very beneficial to improving the safety of the products.

D. Recycling of reagents and waste treatment. In the extraction process of nicotine, the methyl acetate obtained by evaporating the solvent and recovering can be used for extracting the nicotine again. As the tobacco is a potassium-loving and chlorine-aversion plant, a large amount of potassium sulfate needs to be applied in the tobacco cultivation process to improve the quality of the tobacco, the soil acidity can be caused after the potassium sulfate is applied, and the adjustment needs to be carried out by lime. The residue after extracting the nicotine and the extraction raffinate can be directly returned to the tobacco field as fertilizer, so that the waste is fully and comprehensively applied.

The second purpose of the invention is realized by the application of the nicotine with low content of the hypoalkaloids in preparing buccal cigarettes, electronic cigarettes and heating non-combustible cigarettes with low content of nitrosamine.

Compared with nicotine prepared by the traditional method, the nicotine with low content of the secondary alkaloid has no negative influence on the sensory quality of the product, and the tobacco-specific nitrosamine content of the product is obviously reduced.

The nicotine with low content of hypoalkaloids is prepared by a method for preparing tobacco alkaloids by using twigs newly emerged after tobacco leaves are harvested and cured, after the tobacco leaves are harvested, fertilizer is applied, a plant growth regulator is sprayed to ensure that the twigs fully grow, the grown twigs enter the bud stage, the twigs are harvested and dried in the sun in the field, crude nicotine capable of being extracted by a solvent extraction method is extracted, and pure nicotine can be obtained after molecular distillation and purification. The nicotine can be used as a raw material of novel tobacco products such as buccal cigarettes, electronic cigarettes or heating non-combustible cigarettes. The method for preparing the nicotine has low cost, convenient operation and environmental protection, and the obtained nicotine has good quality.

The invention has the beneficial effects that:

(1) the tobacco branches used as raw materials of the invention have wide sources and low cost, and the invention solves the problem that the sources of wastes (including tobacco leaf reins, dust in the cigarette processing process and the like) in the traditional production process of extracting nicotine cigarettes are limited.

(2) The invention also adopts reasonable methods of fertilization and plant growth regulator regulation, obviously improves the yield of the twigs and the nicotine content in the twigs, and effectively reduces the cost of nicotine extraction; because the branch grows fast, the picking cycle is short, do not influence the planting of the next crop, has promoted the economic benefits that the tobacco planted effectively.

(3) The method has the advantages that the raw material treatment is simple, and the branches are picked from the tobacco stems and laid in the field for drying, so that the branches can be used for extracting the nicotine; moreover, the nicotine extraction process is simple, and the extraction operation can be carried out at the field. The potassium hydroxide solution is used as an extraction solvent, the extract can be recycled, and the waste can be used as a fertilizer (potassium fertilizer) to be returned to the field to be fully and comprehensively applied. Methyl acetate is adopted as an extracting agent, the boiling point of methyl acetate is low, no solvent residue is left after distillation and recovery, the risk of product solvent residue is avoided, methyl acetate can be reused after distillation and recovery, the whole set of extraction process has no pollutant discharge, and the influence and the pollution to the environment are very small.

(4) In the nicotine extracted by the method, the content of the secondary alkaloid except the nicotine is low, and compared with the nicotine obtained by the traditional method, the content of the tobacco-specific nitrosamine is obviously reduced. The application in the novel tobacco products is very beneficial to improving the safety of the products.

(5) The nicotine obtained by the invention is used for novel tobacco products, and the taste of the nicotine is not different from that of the same products of the nicotine extracted by using the traditional method. Moreover, the release amount of nitrosamine in the product is obviously reduced, and the safety of the product is improved. The new method for extracting nicotine provides a broad high-quality nicotine source for novel tobacco products.

Detailed Description

The invention is further illustrated by the following examples, which are not intended to be limiting in any way, and any variations or modifications which are based on the teachings of the present invention are intended to fall within the scope of the invention.

The nicotine with low content of the secondary alkaloid is obtained by extracting twigs newly emitted after flue-cured tobacco leaves are picked as raw materials, and comprises the steps of raw material obtaining, extraction, purification and post-treatment.

The nicotine with low content of the secondary alkaloid is obtained by extracting twigs newly emitted after flue-cured tobacco leaves are picked and cured as raw materials, and comprises the steps of raw material acquisition, extraction, purification and post-treatment, and specifically comprises the following steps:

A. raw material acquisition: after the tobacco plant picks up the flue-cured tobacco leaves, fertilizing, spraying a plant growth regulator, culturing for 25-30 days until the length of the twigs is more than 60cm, and picking and drying when 40-60% of the twigs enter the bud stage to obtain a raw material a;

B. extraction:

1) crushing the raw material a, sieving the crushed raw material a with a 15-30-mesh sieve, adding a potassium hydroxide solution which is 2-5 times of the weight of the raw material a and has a mass percentage concentration of 3-5%, performing ultrasonic extraction at the temperature of 70-80 ℃ for 30-50 min, and performing filter pressing to obtain filter residue b and an extract c; adding a potassium hydroxide solution with the mass percentage concentration of 3-5% and the weight of 1-2 times that of the filter residue b into the filter residue b, ultrasonically washing the filter residue b at room temperature for 10-15 times, and performing filter pressing to obtain a washing liquid d and washing residue j; mixing the extracting solution c and the washing solution d to obtain a material e;

2) adding methyl acetate of 1/4-1/10 weight of the material e into the material e, fully stirring for extraction, and standing for layering to obtain a methyl acetate phase f and an extraction residual liquid g; adding methyl acetate with the weight of 1/10 in g of raffinate into g of raffinate, fully stirring and extracting to obtain a methyl acetate phase h and raffinate k, combining the methyl acetate phase f and the methyl acetate phase h, and recovering a solvent from methyl acetate to obtain crude nicotine i;

C. and (3) purification: carrying out molecular distillation on the crude nicotine i under the protection of nitrogen, and collecting distillation components at 100-120 ℃ to obtain a target pure nicotine;

D. and (3) post-treatment: and returning the waste washing slag j and the raffinate k generated in the extraction step to the tobacco field as fertilizers.

The fertilization in the step A is to apply the three-element compound fertilizer with the mass ratio of nitrogen to phosphorus to potassium of (10-20) to (10-20) and urea.

The application amount of the three-element compound fertilizer is 15-20 kg/mu.

The application amount of the urea is 5-10 kg/mu.

The plant growth regulator in the step A comprises the following components: 0.3-0.5% of compound sodium nitrophenolate, 0.1-0.2% of sodium naphthaleneacetate, 0.001-0.002% of brassinolide, 0.05-0.1% of gibberellin, 2-5% of monopotassium phosphate, 2-5% of ammonium nitrate and the balance of water;

the application of the nicotine with low content of the hypoalkaloids is the application of the nicotine with low content of the hypoalkaloids in preparing buccal cigarettes, electronic cigarettes and heating non-combustible cigarettes with low content of nitrosamine.

All percentages used in the present invention are mass percentages, unless otherwise indicated.

The invention is further illustrated by the following specific examples:

example 1

The tobacco variety is K326, the planting is carried out according to the normal planting rule, and the leaves are collected for baking. Leaves are not picked completely (3-4 leaves on the upper part of each tobacco plant are reserved). After harvesting tobacco leaves, applying fertilizer per mu: 15 kg of three-element compound fertilizer (N, P and K are 15:15: 15) and 10kg of urea. The fertilizer can be uniformly sprinkled in the tobacco ditch to provide sufficient nutrients for the full development of the twigs.

After the fertilizer application, a plant growth regulator (sodium nitrophenolate 0.5%, sodium naphthaleneacetate 0.2%, brassinolide 0.002%, gibberellin 0.6%, potassium dihydrogen phosphate 5%, ammonium nitrate 5%, and the balance water) is prepared. The plant growth regulator is diluted by 20 times with water again, and is sprayed on tobacco plants, and 20kg of diluent is sprayed per mu of plant growth regulator to promote the growth of branches of the tobacco plants.

After the flue-cured tobacco plants are fertilized and sprayed with the plant growth regulator, a large number of branches grow rapidly on the tobacco stems for 25-30 days, the length of the branches can reach more than 60cm, about 50 percent of the branches enter buds and can be harvested. Collecting the branch from the tobacco stem, spreading in the field, and drying in the sun, so as to obtain the product for extracting nicotine. The branch yield (dry weight) of each mu of tobacco field can reach 160 kg. The total alkali content of the plant of the branch reaches 3.4 percent.

Example 2

The tobacco variety is Honghuadajinyuan, which is planted according to the normal planting rules, and the leaves are collected and baked. Leaves are not picked completely (3-4 leaves on the upper part of each tobacco plant are reserved). After harvesting tobacco leaves, applying fertilizer per mu: 15 kg of three-element compound fertilizer (N, P and K are 15:15: 15) and 6 kg of urea. The fertilizer can be uniformly sprinkled in the tobacco ditch to provide sufficient nutrients for the full development of the twigs.

After the fertilizer application, a plant growth regulator (sodium nitrophenolate 0.3%, sodium naphthaleneacetate 0.15%, brassinolide 0.015%, gibberellin 0.5%, potassium dihydrogen phosphate 3%, ammonium nitrate 4%, and the balance water) is prepared. The plant growth regulator is diluted by 20 times with water again, and is sprayed on tobacco plants, and 18 kg of diluent is sprayed per mu of plant growth regulator to promote the growth of branches of the tobacco plants.

After the flue-cured tobacco plants are fertilized and sprayed with the plant growth regulator, a large number of branches grow rapidly on the tobacco stems for 25-30 days, the length of the branches can reach more than 60cm, about 50 percent of the branches enter buds and can be harvested. Collecting the branch from the tobacco stem, spreading in the field, and drying in the sun, so as to obtain the product for extracting nicotine. The branch yield (dry weight) of each mu of tobacco field can reach 140 kg. The total alkali content of the plant of the branch reaches 3.0 percent.

Example 3

The branches obtained in example 1 were crushed to 20 mesh, placed in an ultrasonic extraction tank, and then 3% potassium hydroxide solution, 3 times the weight of the branches, was added to the ultrasonic extraction tank, and ultrasonic extraction was carried out at 75 ℃ for 40 min. After extraction, the extract is filtered by pressure. Adding 3% potassium hydroxide solution 1.5 times the weight of the branch into the extraction tank again, ultrasonically washing the branch powder at room temperature for 10 min, and press-filtering to obtain washing liquid. Mixing the extractive solution and the washing solution for extracting nicotine.

Putting the extract liquor into a liquid separating device, adding methyl acetate with the weight of 1/6 of the extract liquor, fully stirring for extraction, then standing for layering, and separating out a methyl acetate phase; adding 1/10 methyl acetate into the extraction residual liquid again, and extracting once again; and combining the methyl acetate phases extracted twice, and evaporating the methyl acetate to dryness to obtain crude nicotine with the plant total alkali content of 94.2%.

Example 4

The twigs obtained in example 2 were crushed to 15 mesh, placed in an ultrasonic extraction tank, and then 3% potassium hydroxide solution in an amount 4 times the weight of the twigs was added to the ultrasonic extraction tank, and subjected to ultrasonic extraction at 80 ℃ for 40 min. After extraction, the extract is filtered by pressure. Adding 3% potassium hydroxide solution 1.6 times the weight of the branch into the extraction tank again, ultrasonically washing the branch powder at room temperature for 10 min, and press-filtering to obtain washing liquid. Mixing the extractive solution and the washing solution for extracting nicotine.

Putting the extract liquor into a liquid separating device, adding methyl acetate with the weight of 1/5 of the extract liquor, fully stirring for extraction, then standing for layering, and separating out a methyl acetate phase; adding 1/10 methyl acetate into the raffinate, and extracting once again; and combining the methyl acetate phases extracted twice, and evaporating the methyl acetate to dryness to obtain crude nicotine with the plant total alkali content of 93.8%.

Example 5

The crude nicotine obtained in the embodiments 3 and 4 is subjected to molecular distillation under the protection of nitrogen, and distillation components at 100-120 ℃ are collected, so that pure nicotine with plant total alkali content of 98.8% and 97.9% respectively can be obtained. The pure nicotine is measured by gas chromatography-mass spectrometry, the content of nicotine exceeds 96%, and the proportion of other tobacco secondary alkaloids does not exceed 4%. The method adopts the liquid chromatography-tandem mass spectrometry to determine the tobacco-specific nitrosamine in the nicotine, and compared with the nicotine extracted from the broken tobacco in the cigarette processing by the traditional method, the tobacco-specific nitrosamine is reduced by more than 40 percent.

Example 6

The nicotine purified in example 3 and example 5 was used for buccal cigarette. The mass formula of each component in the buccal cigarette is as follows in grams: 4500 parts of maltitol, 2500 parts of isomaltitol, 35 parts of edible hydrogenated oil, 35 parts of citric acid, 12 parts of mint essence, 10 parts of a coloring agent and 300 parts of partially obtained crude nicotine. Mixing maltitol and tobacco water extract, dissolving, heating to just boil, adding isomaltitol gradually, stirring, mixing, introducing into sugar solution tank, decocting under normal pressure to obtain viscous liquid with water content of below 2%, and making the sugar solution be in boiling state. Cooling the boiled sugar solution to about 100 ℃, adding edible hydrogenated oil, citric acid and colorant, and blending uniformly. Further cooling to remove air bubbles, pouring or punching into a die, forming particles, demolding, cooling, entering a packaging area, and packaging to obtain the finished product. The taste of the finished product is not different from that of buccal cigarette containing nicotine extracted by a traditional method. The method adopts the liquid chromatography-tandem mass spectrometry to determine the tobacco-specific nitrosamine in the finished product, and compared with the nicotine extracted from the shredded tobacco in the traditional method, the tobacco-specific nitrosamine is reduced by over 46 percent.

Example 7

The nicotine purified from example 4 in example 5 was used as electronic cigarette liquid. The tobacco tar formula comprises the following components in parts by weight: the electronic cigarette tobacco tar is obtained by uniformly mixing and stirring 1 part of compound formula traditional Chinese medicine extract, 10 parts of propylene glycol, 80 parts of glycerol, 5 parts of crude nicotine prepared by the method and 3.5 parts of tobacco essence at room temperature. The tobacco tar is used for electronic cigarettes, and the taste of the tobacco tar is not different from that of nicotine buccal cigarettes extracted by a traditional method. The release amount of the nitrosamine in the smoke in the finished product is determined by adopting a liquid chromatography-tandem mass spectrometry method, and compared with the nicotine extracted from the broken tobacco in the cigarette processing by using the traditional method, the specific nitrosamine in the tobacco is reduced by over 42 percent.

Example 8

The nicotine purified from example 3 in example 5 was used for heating non-combustible smoke. The formula of the smoking particles for the cigarette which is not burnt by heating comprises the following components in parts by weight: 8 parts of microcrystalline cellulose, 80 parts of tea powder, 0.9 part of a flue-cured tobacco pyrolysis product of Zimbabwe at 280 ℃, 0.5 part of nicotine extracted by the method, 8 parts of vegetable glycerol, 0.1 part of tobacco essence and 2 parts of propylene glycol. Drying and grinding tea leaves, controlling the water content at 9.5% at 60 ℃, and then sieving. Taking tea powder with the granularity of 50-80 meshes as a raw material, fully and uniformly mixing the tea powder with microcrystalline cellulose, a cured tobacco pyrolysis product of Zimbabwe at 280 ℃, nicotine, vegetable glycerin, tobacco essence and propylene glycol, and preparing a tobacco granule semi-finished product. Drying the obtained tobacco particle semi-finished product under the conditions that the vacuum degree is 20-40 Pa and the temperature is 50-65 ℃, wherein the moisture content of the dried particles is 11.5 percent, and the tobacco smoking particle finished product for the cigarette is obtained by heating and non-burning. The granule is used for smoking non-combustible cigarette, and has no difference in taste compared with buccal cigarette containing nicotine extracted by conventional method. The release amount of the nitrosamine in the smoke in the finished product is determined by adopting a liquid chromatography-tandem mass spectrometry method, and compared with the nicotine extracted from the broken tobacco in the cigarette processing by using the traditional method, the release amount of the nitrosamine in the tobacco is reduced by more than 46 percent.