1. The preparation method of the microbial environment modifier is characterized by comprising the following steps of:

step one, mixing 5-6 parts of glucose, 8-12 parts of soybean peptone and 1-2 parts of magnesium sulfate heptahydrate according to parts by mass to obtain a mixture A; setting the temperature to be 20-22 ℃, and stirring the mixture A at room temperature to obtain a mixture B;

step two, performing ultrasonic treatment on the mixture B stirred at room temperature; sieving after the ultrasonic treatment is finished to obtain a mixture C; dissolving the sieved mixture C in distilled water, and conventionally sterilizing to obtain a yeast culture medium;

inoculating the yeast into a yeast culture medium, performing oscillation activation culture at constant temperature of 30 ℃ and at 200rpm of 160-;

step four, preparing an LB liquid culture medium and a Bacillus subtilis fermentation culture medium, inoculating a Bacillus subtilis strain into an LB activated culture medium, carrying out shaking culture at constant temperature of 37 ℃ and 180rpm for 22-24h, inoculating the activated bacteria liquid into the Bacillus subtilis fermentation culture medium according to the volume ratio of 2-5%, and carrying out shaking culture at constant temperature of 37 ℃ and 130 rpm and 160rpm for 48h to obtain the Bacillus subtilis liquid for expanded culture;

dissolving 6-7 parts of glucose, 3-4 parts of peptone, 2 parts of yeast powder, 1 part of trisodium citrate and 1 part of potassium dihydrogen phosphate in 22-25 parts of distilled water, adjusting the pH value to 7.0-7.3, and performing conventional sterilization to obtain a lactobacillus plantarum culture medium;

step six, inoculating the lactobacillus plantarum into a lactobacillus plantarum culture medium, and firstly performing activated culture under the culture conditions: carrying out shaking culture at constant temperature of 37 ℃ and 150rpm for 24 h; inoculating the activated bacterium liquid into a lactobacillus plantarum culture medium according to the volume ratio of 2-5%, and carrying out shake culture at constant temperature of 35-37 ℃ and at the speed of 120-150rpm for 24-48h to obtain the lactobacillus plantarum bacterium liquid subjected to enlarged culture;

seventhly, preparing a wort culture medium by using wort with the sugar degree of 8Be +/-0.5, and adjusting the pH value of the wort culture medium to Be 5.6-5.9; adding 1% glycerol by volume into the wort culture medium, uniformly oscillating, placing the glycerol-containing wort culture medium in a triangular flask, sealing with gauze, and wrapping with kraft paper;

step eight, setting the temperature to be 80-96 ℃ and the pressure to be 0.1Mpa, and carrying out steam sterilization on the sealed triangular flask for 25-30 min; preserving heat for 20-25min after sterilization; placing the sterilized triangular flask in a sterile room to obtain a streptococcus lactis culture medium;

step nine, inoculating the streptococcus lactis into a streptococcus lactis culture medium, and performing activation culture firstly, wherein the culture conditions are as follows: carrying out shaking culture at constant temperature of 35 ℃ and 120rpm for 24 h; inoculating the activated bacteria liquid into a streptococcus lactis culture medium according to the volume ratio of 2-5%, and carrying out shaking culture at constant temperature of 30-35 ℃ and 100-120rpm for 24-48h to obtain the streptococcus lactis bacteria liquid subjected to expanded culture;

step ten, the yeast expanded culture bacterial liquid obtained in the step three, the bacillus subtilis expanded culture bacterial liquid obtained in the step four, the lactobacillus plantarum expanded culture bacterial liquid obtained in the step six, and the streptococcus lactis expanded culture bacterial liquid obtained in the step nine are mixed according to the mass part or volume ratio of 2-3: 1-3: 2-4: 1-2, standing for 1-2h to obtain the microbial environment modifier.

2. The method for preparing a microbial environment improver according to claim 1, wherein in the second step, the mixture B stirred at room temperature is subjected to ultrasonic treatment, the ultrasonic treatment frequency is 20 to 23kHz, and the ultrasonic treatment time is 6 to 8 min.

3. The method for preparing a microbial environment improver according to claim 1, wherein in the third step, the yeast is inoculated into a yeast culture medium for activation culture, the temperature is set to 30 ℃, and the culture time is 18-22 h; inoculating the activated product into a fermentation tank containing a yeast culture medium according to the volume ratio of 2-4% for secondary culture, wherein the culture temperature is 28-30 ℃, and the culture time is 24-48 h.

4. The method for preparing a microbial environment improver according to claim 1, wherein in the fourth step, the preparation of the bacillus subtilis fermentation medium comprises: mixing 6-8 parts of starch, 3-5 parts of glucose, 1-2 parts of yeast powder, 1 part of monopotassium phosphate, 0.5-1 part of sodium chloride and 0.5 part of ascorbic acid, dissolving in 20-30 parts of distilled water, adjusting the pH value to 7.0-7.2, and conventionally sterilizing.

5. The microbial environment improver prepared by the preparation method of the microbial environment improver according to claims 1 to 4, wherein the microbial environment improver comprises 2 to 3 parts of yeast after amplification culture, 1 to 3 parts of bacillus subtilis, 2 to 4 parts of lactobacillus plantarum and 1 to 2 parts of streptococcus lactis according to the mass parts or volume ratio.

6. The microbial bedding material prepared by applying the microbial environment improver as claimed in claim 5, is characterized by comprising 15-20 parts of bio-organic material, 1-2 parts of charcoal, 0.1-0.5 part of montmorillonite, 0.5-1 part of water retaining agent, 0.5-1 part of tea tree oil and 2-3 parts of microbial environment improver in parts by mass.

7. A method of preparing a microbial litter of claim 6 wherein the method comprises the steps of:

(1) preparing an organic material by using crushed wheat straws, sawdust and diatomite powder, wherein the mass part ratio of the wheat straws, the sawdust and the diatomite powder is 5:3: 2;

(2) crushing charcoal to obtain charcoal fragments with the particle size of less than 3 mm;

(3) pulverizing montmorillonite (montmorillonite), grinding, ball milling in a ball mill, and sieving with 60-80 mesh sieve to obtain montmorillonite powder;

(4) adding organic materials, charcoal scraps, montmorillonite powder and a water-retaining agent into a stirrer, uniformly stirring, adding tea tree oil, and stirring again to uniformly adhere the tea tree oil;

(5) and adding the microbial environment modifier into the stirrer, and uniformly stirring to obtain the microbial padding.

8. The method for preparing microbial bedding material as claimed in claim 7, wherein in the step (1), the step of preparing the organic material by using the wheat straw, the sawdust and the diatomite powder comprises the following steps:

1) cutting dried wheat straw into pieces by a cutting machine, and then crushing into powder by a crusher;

2) crushing and grinding diatomite, then placing the crushed and ground diatomite into a ball mill for ball milling, and sieving the ball mill by a sieve of 80-100 meshes to obtain diatomite powder;

3) mixing wheat straw powder, sawdust and diatomite powder.

9. The method for preparing microbial bedding material of claim 7, wherein in the step (1), the organic material is prepared by mixing 50% of wheat straw powder, 30% of sawdust and 20% of diatomite powder.

Background

The microbial fermentation bedding material bed culture technology is an environment-friendly, safe and effective ecological culture method provided by utilizing a brand-new natural agricultural concept and combining a modern microbial fermentation treatment technology. The microbial fermentation bedding bed cultivation integrates cultivation science, microbiology, nutriology, environmental sanitation and soil fertilizer science, organically combines the microbial technology, the fermentation technology and the cultivation technology, decomposes the excrement and urine of the livestock and the poultry by using microbes, eliminates odor, realizes the in-situ absorption of the excrement and urine at the source, purifies the environment, improves the absorption efficiency of the livestock and the poultry on the nutrition of the feed and the immunity of organisms, is a virtuous-cycle ecological cultivation system established according to the principles of low cost, high yield and no pollution, and is a new mode of sustainable development of the cultivation industry.

In the process of culturing the microbial padding, firstly, selected functional microorganisms are cultured and propagated to form a microbial mother strain with considerable activity, and then the microbial mother strain, sawdust, rice husks, straw powder and the like, a certain amount of auxiliary materials and an active agent are mixed according to a certain formula to form the organic padding. The organic padding is laid in a colony house constructed according to certain design requirements, and then the animals are placed on a microbial fermentation padding bed of the colony house, so that the animals live on the organic padding from small to large. The excrement of the animals is degraded and digested by microorganisms in the organic padding, so that the excrement of the animals does not need to be manually treated, and excrement cleaning, storing and treating facilities are not needed. Meanwhile, the breeding colony house with the microbial fermentation bedding bed provides an optimum ecological environment for the growth of livestock and poultry, the livestock and poultry grow fast in the environment, the yield is high, the quality is good, the diseases are few, the labor, the water, the disinfection drug cost, the coal and carbon are saved, and the like, and the breeding benefit of the livestock and poultry can be obviously improved while the environmental pollution is not caused.

The core component of the microbial fermentation bedding material bed is the proportion and the composition of functional microbes and bedding materials. The functional microorganism is a huge beneficial microorganism complex, the artificially prepared beneficial microbial live bacteria preparation takes livestock and poultry dung and urine which are not completely digested as feed by the thallus through the adsorption effect and natural fermentation of padding, the dung and urine excreted by the livestock and the poultry are fermented, assimilated and decomposed, and meanwhile, nutrient hypha containing rich mycoprotein and digestive enzyme is formed and is eaten by the livestock. In the process, beneficial functional microorganisms can eliminate odor, inhibit breeding of pests and germs and enhance the disease resistance of livestock by digesting the feces and urine of the livestock in situ, meanwhile, microorganisms which propagate and grow in large quantities can provide edible nutrient substances such as saccharides, proteins, organic acids, vitamins and the like for the livestock, under the combined action of the beneficial microorganisms and the powerful complex enzyme, the intestinal digestion system of animals can reach the optimal state, and the complex enzyme degrades crude fibers in the feed and destroys cell walls of the feed, so that the animals can absorb the nutrient substances to the greatest extent, and the production purposes of friendly surrounding environment, reduction of labor investment, cost saving and improvement of economic benefit are achieved while secondary conversion, absorption and utilization of the feed are realized.

In a microbial fermentation bedding culture system, the species composition of functional microbes is a core factor, and determines the decomposition speed and the digestion quantity of excrement and urine discharged by livestock and poultry; the padding is also an important component of the fermentation bed body, is a culture medium for the growth and the propagation of microorganisms and a carrier for inhabitation, and can directly influence the decomposition process of the strains on the excrement in the aspects of providing a carbon source, ensuring the ventilation volume, maintaining the temperature of the bed body and the like. The currently commonly used microorganism species include bacillus, saccharomycetes, filamentous fungi, actinomycetes, photosynthetic bacteria, lactic acid bacteria and the like, and the feces decomposition effect of the combination of different functional microorganisms is greatly different; the padding materials commonly used at present mainly comprise plant tissue materials such as corn straws, peanut shells, rice hulls, sawdust, bran and the like. Among the reported components of the microbial fermentation bedding, the current situations that the types of functional microorganisms are few, and bedding components are mainly single plant tissue materials generally exist, so that the microbial fermentation bedding has the phenomena of poor sterilization, deodorization and mildew prevention effects, low decomposition efficiency of livestock manure, and the like. Therefore, the development of a novel functional microbial environment modifier and a microbial fermentation padding for livestock and poultry cultivation are urgently needed.

Through the above analysis, the problems and defects of the prior art are as follows: among the reported components of the microbial fermentation bedding, the current situations that the types of functional microorganisms are few, and bedding components are mainly single plant tissue materials generally exist, so that the microbial fermentation bedding has the phenomena of poor sterilization, deodorization and mildew prevention effects, low decomposition efficiency of livestock manure, and the like.

Disclosure of Invention

Aiming at the problems and the defects in the prior art, the invention provides a microbial environment modifier and a microbial padding for livestock and poultry breeding.

The invention is realized in such a way that the preparation method of the microbial environment modifier comprises the following steps:

step one, mixing 5-6 parts of glucose, 8-12 parts of soybean peptone and 1-2 parts of magnesium sulfate heptahydrate according to parts by mass to obtain a mixture A; setting the temperature to be 20-22 ℃, and stirring the mixture A at room temperature to obtain a mixture B;

step two, performing ultrasonic treatment on the mixture B stirred at room temperature; sieving after the ultrasonic treatment is finished to obtain a mixture C; dissolving the sieved mixture C in distilled water, and conventionally sterilizing to obtain a yeast culture medium;

inoculating the yeast into a yeast culture medium, performing oscillation activation culture at constant temperature of 30 ℃ and at 200rpm of 160-;

and step four, preparing an LB liquid culture medium and a Bacillus subtilis fermentation culture medium, inoculating a Bacillus subtilis strain into an LB activation culture medium, carrying out shaking culture at constant temperature of 37 ℃ and 180rpm for 22-24h, inoculating the activated bacterium liquid into the Bacillus subtilis fermentation culture medium according to the volume ratio of 2-5%, and carrying out shaking culture at constant temperature of 37 ℃ and 130 rpm for 48h to obtain the expanded-culture Bacillus subtilis liquid.

Dissolving 6-7 parts of glucose, 3-4 parts of peptone, 2 parts of yeast powder, 1 part of trisodium citrate and 1 part of potassium dihydrogen phosphate in 22-25 parts of distilled water, adjusting the pH value to 7.0-7.3, and performing conventional sterilization to obtain a lactobacillus plantarum culture medium;

step six, inoculating the lactobacillus plantarum into a lactobacillus plantarum culture medium, and firstly performing activated culture under the culture conditions: carrying out shaking culture at constant temperature of 37 ℃ and 150rpm for 24 h; inoculating the activated bacterium liquid into a lactobacillus plantarum culture medium according to the volume ratio of 2-5%, and carrying out shake culture at constant temperature of 35-37 ℃ and at the speed of 120-150rpm for 24-48h to obtain the lactobacillus plantarum bacterium liquid for enlarged culture.

Seventhly, preparing a wort culture medium by using wort with the sugar degree of 8Be +/-0.5, and adjusting the pH value of the wort culture medium to Be 5.6-5.9; adding 1% glycerol by volume into the wort culture medium, uniformly oscillating, placing the glycerol-containing wort culture medium in a triangular flask, sealing with gauze, and wrapping with kraft paper;

step eight, setting the temperature to be 80-96 ℃ and the pressure to be 0.1Mpa, and carrying out steam sterilization on the sealed triangular flask for 25-30 min; preserving heat for 20-25min after sterilization; placing the sterilized triangular flask in a sterile room to obtain a streptococcus lactis culture medium;

step nine, inoculating the streptococcus lactis into a streptococcus lactis culture medium, and performing activation culture firstly, wherein the culture conditions are as follows: carrying out shaking culture at constant temperature of 35 ℃ and 120rpm for 24 h; inoculating the activated bacteria liquid into a streptococcus lactis culture medium according to the volume ratio of 2-5%, and carrying out shaking culture at constant temperature of 30-35 ℃ and 100-120rpm for 24-48h to obtain the streptococcus lactis bacteria liquid for expanded culture.

Step ten, the yeast expanded culture bacterial liquid obtained in the step three, the bacillus subtilis expanded culture bacterial liquid obtained in the step four, the lactobacillus plantarum expanded culture bacterial liquid obtained in the step six, and the streptococcus lactis expanded culture bacterial liquid obtained in the step nine are mixed according to the mass part or volume ratio of 2-3: 1-3: 2-4: 1-2, standing for 1-2h to obtain the microbial environment modifier.

Further, in the second step, the ultrasonic treatment frequency is 20-23kHz, and the ultrasonic treatment time is 6-8 min.

Further, in the third step, the conditions for inoculating the yeast into the yeast culture medium for activation culture are as follows: setting the culture temperature at 30 ℃, the rotation speed of a shaking table at 160-; the temperature of the yeast in the fermentation tank is 28-30 ℃, and the culture time is 24-48 h.

Further, in the fourth step, the preparation of the bacillus subtilis culture medium comprises: mixing 6-8 parts of starch, 3-5 parts of glucose, 1-2 parts of yeast powder, 1 part of monopotassium phosphate, 0.5-1 part of sodium chloride and 0.5 part of ascorbic acid, dissolving in 20-30 parts of distilled water, adjusting the pH value to 7.0-7.2, and conventionally sterilizing.

The invention aims to provide a microbial environment modifier prepared by applying the preparation method of the microbial environment modifier, and the microbial environment modifier comprises 2-3 parts of yeast after amplification culture, 1-3 parts of bacillus subtilis, 2-4 parts of lactobacillus plantarum and 1-2 parts of streptococcus lactis in parts by mass.

The microbial padding is prepared from 15-20 parts of bio-organic materials, 1-2 parts of charcoal, 0.1-0.5 part of montmorillonite, 0.5-1 part of water-retaining agents, 0.5-1 part of tea tree oil and 2-3 parts of microbial environment modifiers according to parts by mass.

Another object of the present invention is to provide a method for preparing a microbial bedding using the microbial bedding, which comprises the following steps:

(1) preparing organic materials by using crushed wheat straws, sawdust and diatomite powder;

(2) crushing charcoal to obtain charcoal fragments with the particle size of less than 3 mm;

(3) pulverizing montmorillonite (montmorillonite), grinding, ball milling in a ball mill, and sieving with 60-80 mesh sieve to obtain montmorillonite powder;

(4) adding organic materials, charcoal scraps, montmorillonite powder and a water-retaining agent into a stirrer, uniformly stirring, adding tea tree oil, and stirring again to uniformly adhere the tea tree oil;

(5) and adding the microbial environment modifier into the stirrer, and uniformly stirring to obtain the microbial padding.

Further, in the step (1), the preparation of the organic material by using the crushed wheat straws, the sawdust and the diatomite powder comprises the following steps:

1) cutting dried wheat straw into pieces by a cutting machine, and then crushing into powder by a crusher;

2) crushing and grinding diatomite, then placing the crushed and ground diatomite into a ball mill for ball milling, and sieving the ball mill by a sieve of 80-100 meshes to obtain diatomite powder;

3) mixing wheat straw powder, sawdust and diatomite powder.

Further, in the step (1), the organic material is prepared by mixing 50% of wheat straw powder, 30% of sawdust and 20% of diatomite powder.

By combining all the technical schemes, the invention has the advantages and positive effects that: according to the microbial environment improver and the microbial padding for livestock and poultry cultivation, provided by the invention, the strain compatibility in the microbial environment improver is selected, and the bacterium liquid combination proportion is optimized, so that the microbial community activity in the prepared microbial environment improver is better, and the effect on soil improvement is better; diatomite powder, charcoal, montmorillonite and tea tree oil are added in the preparation of the microbial padding, so that the adsorption effect on microorganisms is better, the water absorption and deodorization effects on livestock and poultry manure are stronger, the mildew removal efficiency on a culture fermentation padding bed and the prevention efficiency on animal intestinal diseases are higher, and the environment purification effect on a livestock and poultry culture area is better; the prepared microbial padding can improve the immune function of animals and ensure the healthy growth of the animals; and the padding can be used as a high-quality organic fertilizer for crops after being used, so that the high-efficiency cyclic utilization of resources is promoted, and the green sustainable development of agriculture is realized.

The invention starts from the organic combination of functional microorganisms, firstly provides an environment modifier capable of improving the soil fertility, adds beneficial components of non-plant tissues in the padding composition, mainly solves the problems of improving the sterilization and deodorization effects of a microbial fermentation padding bed, the prevention effect of animal intestinal diseases, the decomposition efficiency of livestock and poultry manure and the like, and simultaneously enlarges the livestock and poultry breeding application range of the microbial fermentation padding bed.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required to be used in the embodiments of the present application will be briefly described below. It is obvious that the drawings described below are only some embodiments of the application, and that for a person skilled in the art, other drawings can be obtained from these drawings without inventive effort.

FIG. 1 is a flow chart of a method for preparing a microbial environment improver according to an embodiment of the present invention.

FIG. 2 is a flow chart of a method for preparing a culture medium for yeast according to an embodiment of the present invention.

FIG. 3 is a flow chart of a method for inoculating Lactobacillus plantarum in a Lactobacillus plantarum culture medium for culture according to an embodiment of the present invention.

FIG. 4 is a flowchart of a method for sterilizing a culture medium for Streptococcus lactis according to an embodiment of the present invention.

Fig. 5 is a flow chart of a method for preparing a microbial bedding material according to an embodiment of the invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Aiming at the problems in the prior art, the invention provides a microbial environment modifier and a microbial pad, and the invention is described in detail below with reference to the accompanying drawings.

The microbial environment modifier provided by the embodiment of the invention is composed of 2-3 parts of yeast, 1-3 parts of bacillus subtilis, 2-4 parts of lactobacillus plantarum and 1-2 parts of streptococcus lactis according to the mass parts or volume ratio.

As shown in fig. 1, the preparation method of the microbial environment improver provided by the embodiment of the present invention includes the following steps:

s101, preparing a yeast culture medium, inoculating yeast strains into the yeast culture medium, performing constant temperature 30 ℃ and vibration activation culture at 160-200rpm for 18-22h, inoculating the activated product into a fermentation tank containing the yeast culture medium according to the volume ratio of 2-4% for 24-48h for re-culture, and obtaining yeast liquid for enlarged culture at the culture temperature of 28-30 ℃.

S102, preparing an LB liquid culture medium and a Bacillus subtilis fermentation culture medium, inoculating a Bacillus subtilis strain into an LB activated culture medium, carrying out shaking culture at constant temperature of 37 ℃ and 180rpm for 22-24h, inoculating the activated bacteria liquid into the Bacillus subtilis fermentation culture medium according to the volume ratio of 2-5%, and carrying out shaking culture at constant temperature of 37 ℃ and 130 rpm and 160rpm for 48h to obtain the expanded-culture Bacillus subtilis liquid.

S103, dissolving 6-7 parts of glucose, 3-4 parts of peptone, 2 parts of yeast powder, 1 part of trisodium citrate and 1 part of potassium dihydrogen phosphate in 22-25 parts of distilled water, adjusting the pH value to 7.0-7.3, and performing conventional sterilization to obtain a lactobacillus plantarum culture medium; inoculating the activated lactobacillus plantarum strain liquid into a lactobacillus plantarum culture medium according to the volume ratio of 2-5%, and carrying out shaking culture at constant temperature of 35-37 ℃ and 120-150rpm for 24-48h to obtain the lactobacillus plantarum strain liquid subjected to enlarged culture.

S104, preparing a wort culture medium by using wort with the sugar degree of 8Be +/-0.5, and adjusting the pH value of the wort culture medium to Be 5.6-5.9; adding 1% glycerol by volume into the wort culture medium, shaking uniformly, and sterilizing to obtain the Streptococcus lactis culture medium.

S105, inoculating the streptococcus lactis into a streptococcus lactis culture medium, and performing activation culture under the culture conditions: carrying out shaking culture at constant temperature of 35 ℃ and 120rpm for 24 h; inoculating the activated bacteria liquid into a streptococcus lactis culture medium according to the volume ratio of 2-5%, and carrying out shaking culture at constant temperature of 30-35 ℃ and 100-120rpm for 24-48h to obtain the streptococcus lactis bacteria liquid for expanded culture.

S106, performing mass part or volume ratio of the yeast liquid, the bacillus subtilis liquid, the lactobacillus plantarum liquid and the streptococcus lactis liquid which are subjected to enlarged culture to 2-3: 1-3: 2-4: 1-2, standing for 1-2h to obtain the microbial environment modifier.

As shown in fig. 2, in step S101 provided in the embodiment of the present invention, the preparation of the yeast culture medium includes:

s201, mixing 5-6 parts of glucose, 8-12 parts of soybean peptone and 1-2 parts of magnesium sulfate heptahydrate according to parts by mass to obtain a mixture;

s202, setting the temperature to be 20-22 ℃, and stirring the mixture at room temperature;

s203, carrying out ultrasonic treatment on the mixture stirred at room temperature, wherein the ultrasonic treatment frequency is 20-23kHz, and the ultrasonic treatment time is 6-8 min;

and S204, sieving after the ultrasonic treatment is finished, dissolving the sieved mixture in distilled water, and conventionally sterilizing to obtain the yeast culture medium.

In step S102 provided in the embodiment of the present invention, the preparing of the bacillus subtilis fermentation medium includes: mixing 6-8 parts of starch, 3-5 parts of glucose, 1-2 parts of yeast powder, 1 part of monopotassium phosphate, 0.5-1 part of sodium chloride and 0.5 part of ascorbic acid, dissolving in 20-30 parts of distilled water, adjusting the pH value to 7.0-7.2, and conventionally sterilizing.

As shown in fig. 3, in step S103, the lactobacillus plantarum is inoculated into a lactobacillus plantarum culture medium for cultivation, which includes:

s301, dissolving 6-7 parts of glucose, 3-4 parts of peptone, 2 parts of yeast powder, 1 part of trisodium citrate and 1 part of potassium dihydrogen phosphate in 22-25 parts of distilled water, adjusting the pH value to 7.0-7.3, and performing conventional sterilization to obtain a lactobacillus plantarum culture medium;

s302, inoculating the lactobacillus plantarum into a lactobacillus plantarum culture medium, and firstly performing activated culture under the culture conditions: carrying out shaking culture at constant temperature of 37 ℃ and 150rpm for 24 h;

s303, inoculating the activated and cultured bacterial liquid into a lactobacillus plantarum culture medium according to the volume ratio of 2-5%, and carrying out shaking culture at constant temperature of 35-37 ℃ and at the speed of 120-150rpm for 24-48h to obtain the lactobacillus plantarum bacterial liquid subjected to enlarged culture. As shown in fig. 4, in step S104 provided in the embodiment of the present invention, the sterilization process includes:

s401, placing a wort culture medium containing 1% of glycerol into a triangular flask, sealing the triangular flask by using gauze, and wrapping by using kraft paper;

s402, setting the temperature to be 80-96 ℃ and the pressure to be 0.1Mpa, and carrying out steam sterilization on the sealed triangular flask for 25-30 min;

s403, preserving heat for 20-25min after sterilization is finished;

and S404, placing the sterilized triangular flask in a sterile room to obtain the streptococcus lactis culture medium.

The microbial padding provided by the embodiment of the invention comprises, by mass, 15-20 parts of a biological organic material, 1-2 parts of charcoal, 0.1-0.5 part of montmorillonite, 0.5-1 part of a water-retaining agent, 0.5-1 part of tea tree oil and 2-3 parts of a microbial environment modifying agent.

As shown in fig. 5, a method for preparing a microbial bedding provided by an embodiment of the present invention includes the following steps:

s501, preparing an organic material by using crushed wheat straws, sawdust and diatomite powder, wherein the mass part ratio of the wheat straws, the sawdust and the diatomite powder is 5:3: 2;

s502, smashing charcoal to obtain charcoal fragments with the particle size less than 3 mm;

s503, grinding montmorillonite (montmorillonite), placing the ground montmorillonite into a ball mill for ball milling, and sieving by a sieve of 60-80 meshes to obtain montmorillonite powder;

s504, respectively adding an organic material, charcoal scraps, montmorillonite powder and a water-retaining agent into a stirrer in proportion, uniformly stirring, adding tea tree oil in proportion, and stirring again to uniformly adhere the tea tree oil;

and S505, adding the microbial environment modifier into the stirrer in proportion, and uniformly stirring to obtain the microbial padding.

In step S501 provided in the embodiment of the present invention, preparing an organic material using crushed wheat straw, sawdust and diatomite powder includes: cutting dried wheat straw into pieces by a cutting machine, and then crushing into powder by a crusher; pulverizing diatomite, grinding, ball milling in a ball mill, and sieving with 80-100 mesh sieve; mixing wheat straw powder, sawdust and diatomite powder.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein should be covered within the scope of the present invention.