1. The application of the water-based UV curing resin in the sealing piece.

2. The use according to claim 1, wherein the aqueous UV-curable resin is a UV-curable resin, and an aqueous group or segment is introduced to make the resin hydrophilic and water-absorbing.

3. Use according to claim 1, wherein the aqueous UV curable resin is an unsaturated polyester resin, a urethane acrylate or an epoxy acrylate.

4. The sealing method is characterized in that a sample is sealed by using water-based UV curing resin and is cured by ultraviolet irradiation.

5. The mounting method of claim 4, wherein the sample is a cellular component or a tissue section.

6. A mounting method according to claim 4 or 5, wherein the sample is a stained sample or a non-stained sample.

7. The mounting method of claim 6, wherein the specimen is stained and rinsed with clean water.

8. The method of claim 1, wherein the wavelength of the ultraviolet light is 315nm and 400 nm.

9. The mounting method of claim 1, wherein the uv irradiation time is 10 seconds to 1 minute.

10. The mounting method of claim 1, wherein the ultraviolet light source is less than 10cm from the slide.

Background

In the field of medical detection, cell components or tissue sections are transferred onto glass slides, and are subjected to microscopic observation after being dyed and the like, so that the method is one of important detection means and is very wide in application. To prevent the sample from being exposed to the air and discoloring by oxidation or prevent external contamination, and to enable the sample slide to be preserved for a long time, the area of the slide where the sample is located needs to be sealed by a suitable method, and the process is called sealing.

The commonly used mounting method at present is to use organic solvent such as ethanol, xylene and the like to dehydrate and remove ethanol from the sample area of the glass slide, then to drop neutral gum or mounting gum, and then to stick the cover glass to mount the glass slide. After the glue is cured, a sandwich structure with two surfaces made of glass and a transparent sealing layer in the middle is formed, and finally the sample is sealed in the cured glue. Although the traditional method is widely applied, the defects are obvious. Firstly, the operation steps are complicated, and a large amount of time is consumed; the "dehydration" takes at least 2 or more times to complete, and the dealcoholization is carried out by soaking the chips in xylene for a long time. Secondly, organic solvents such as ethanol and xylene are needed to be used for dehydration and dealcoholization in the sealing process, which pollutes the environment; finally, the curing time is long, at least several hours or even days. The neutral gum dries naturally through cure for at least 36 hours, even with oven drying, for at least 2 hours.

The use of dangerous substances such as ethanol, xylene and highly similar agents is entirely intended to cater for the use of neutral gums, and not for the dyeing itself. Since 75% of the inside of the cell is water, the staining solution is necessarily water-soluble, and can enter the cell for staining. This determines that the matrix of the staining solution is necessarily water or a water-miscible solvent, and methanol, ethanol or the like is generally used. Neutral gums are formulated from canadian gum diluted with xylene and are immiscible with water. Therefore, to perform final encapsulation with neutral gum, the stained intracellular fluid must be replaced with xylene or a solvent miscible with xylene, otherwise, the cells cannot be seen because of the misty or milky appearance caused by the immiscible solvent. The use of organic solvents such as ethanol and xylene can be omitted as long as a suitable substitute for the neutral gum is found.

Therefore, how to provide a new green, environment-friendly, efficient and quick mounting method is a problem which needs to be solved urgently by researchers.

Disclosure of Invention

In view of the above, the present invention provides a novel method for mounting a chip, so as to solve the problems of complicated steps, long time consumption, environmental pollution, etc. of the conventional chip mounting method.

In order to solve the technical problems, the invention provides the following technical scheme:

the invention provides an application of a water-based UV curing resin in a sealing sheet.

The waterborne UV curing resin is formed by introducing a waterborne group or a chain segment on the basis of the UV curing resin, so that the resin has certain hydrophilicity and water absorbability.

Preferably, the aqueous UV curable resin is an unsaturated polyester resin, urethane acrylate or epoxy acrylate.

The invention also provides a sealing method, wherein the sample is sealed by using the aqueous UV curing resin and is cured by ultraviolet irradiation.

Preferably, the sample is a cellular component or a tissue section.

Preferably, the sample is a stained sample or a non-stained sample.

Preferably, the sample is stained and rinsed with clear water.

Preferably, the wavelength of the ultraviolet light is 315-400 nm.

Preferably, the ultraviolet light irradiation time is 10 seconds to 1 minute.

Preferably, the distance between the ultraviolet light source and the glass slide is less than 10 cm.

Compared with the prior art, the invention has the following beneficial effects:

the present invention utilizes aqueous UV curable resins instead of neutral gums for mounting. The water-based UV curing resin is a mature industrial product, has numerous production enterprises and easily obtained raw materials, does not need time-consuming preparation by a user, and is beneficial to large-scale purchase and popularization.

The aqueous UV curing resin contains the ultraviolet light curing primer, so that the aqueous UV curing resin can be cured into a firm transparent resin layer within 10 seconds to 1 minute by using ultraviolet light with certain intensity for short-distance irradiation. The refractive index and the sealing strength of the adhesive are consistent with the curing effect of the neutral gum and even better than the neutral gum.

The solidified sample has bright and clear background, clear nucleus structure, high cell membrane boundary contrast, no color distortion phenomenon and no dissolution and halation of dyeing solution; the effect of the sealant is the same as that of the sealant of the neutral gum, but the background is obviously brighter and the transmittance is better than that of the neutral gum.

The invention completely eliminates dangerous goods such as ethanol, xylene and the like and highly toxic reagents, the operation steps are at least 50 percent omitted compared with the traditional method, and the curing time is shortened to one thousandth of the curing time of the traditional method. The whole process is efficient and quick, green and environment-friendly, and the quality is improved.

Drawings

Detailed Description

The invention provides an application of a water-based UV curing resin in a sealing sheet. The waterborne UV curing resin is formed by introducing a waterborne group or chain segment on the basis of the UV curing resin, so that the resin has certain hydrophilicity and water absorbability. There are two common methods for synthesizing waterborne UV curable resins: one is to introduce hydrophilic groups such as UP (unsaturated polyester resin) into the molecular chain of conventional resins; the other is to react with acrylate to generate water-based acrylate oligomer, such as PUA (polyurethane acrylate), EA (epoxy acrylate) and the like. As a novel, environment-friendly and energy-saving resin, the water-based UV curing resin has the advantages of no solvent odor, no toxicity, no pollution, convenient operation, easy cleaning of residual glue, high solid content, safe and convenient storage and transportation and the like, is the development direction of the environment-friendly and energy-saving UV curing resin, and has various performances superior to those of the traditional UV curing resin. The present invention may adopt any of the above types of aqueous UV curable resins.

The invention provides a sealing method, wherein a sample is sealed by using aqueous UV curing resin and is cured by ultraviolet irradiation. The aqueous UV curable resin is miscible with water or can be diluted with a small amount of water and has a fluidity consistent with that of a neutral gum. In the invention, a few drops of water-based UV curing resin are directly dropped on the sample area, a cover glass is attached, and the water-based UV resin is dispersed between the glass slide and the cover glass to complete the mounting. Because the aqueous UV curing resin contains the ultraviolet light curing primer, the aqueous UV curing resin can be cured into a firm transparent resin layer only by short-distance irradiation of ultraviolet light with certain intensity. In the invention, the wavelength of the ultraviolet light is preferably 315-400nm, more preferably 365-395 nm, and the corresponding wavelength can be flexibly selected according to the specific requirements of production enterprises; the ultraviolet irradiation time is 10 seconds to 1 minute, preferably 30 seconds, and the curing time is short; the distance between the ultraviolet light source and the glass slide is less than 10cm, and 8cm is preferred to ensure the curing effect.

In the present invention, the specimen is preferably a stained cell component or a tissue section. The dyeing method of the present invention is not particularly limited, and dyeing methods known in the art may be used. After the sample is dyed, the sample is washed by clear water, and redundant dyeing liquid is washed away.

The present invention will be described in detail with reference to examples for better understanding the objects, technical solutions and advantages of the present invention, but they should not be construed as limiting the scope of the present invention.

Example 1

And (3) dropwise adding the water-based UV curing resin on the cell sample subjected to dyeing cleaning, attaching a cover glass, and finishing mounting after the water-based UV curing resin is dispersed between the glass slide and the cover glass. Then, the sealed sheet was placed at a distance of 8nm from the UV lamp and irradiated at a wavelength of 315nm for 10 seconds to complete curing.

Example 2

And (3) dropwise adding the water-based UV curing resin on the tissue slice sample subjected to dyeing cleaning, attaching a cover glass, and finishing mounting after the water-based UV curing resin is dispersed between the glass slide and the cover glass. Then, the sealed sheet was placed at a distance of 5nm from the UV lamp and irradiated at a wavelength of 400nm for 30 seconds to complete curing.

Example 3

And (3) dropwise adding the water-based UV curing resin on the tissue slice sample subjected to dyeing cleaning, attaching a cover glass, and finishing mounting after the water-based UV curing resin is dispersed between the glass slide and the cover glass. Then, the sealed sheet was placed at a distance of 9nm from the UV lamp and irradiated at 395nm wavelength for 40 seconds to complete the curing.

Example 4

The refractive index of the cured sample in example 1 can reach 1.6-2.0 at the test wavelength of 390-600nm, which is very consistent with that of the glass slide 1.5-3.3.

Example 5

Taking the same cervical cell sample, preparing two cell smears on average, staining with hematoxylin-eosin, sealing with aqueous UV resin and neutral gum, sealing with aqueous UV resin, curing with neutral resin, and observing with a microscope under 40 times of objective lens.

Specifically, the flow of the two mounting methods is as follows:

the general steps are as follows:

soaking the glass slide with the cervical cells in 95% ethanol for 10 minutes for fixation, then soaking in hematoxylin staining solution for 2 minutes, taking out and washing with clear water, adding 1% hydrochloric acid alcohol for 2 seconds for differentiation, then washing with running water, soaking in eosin staining solution for 10 seconds, and rinsing with running water.

The following are the respective processes of two mounting methods:

1) the method comprises the following steps: directly dripping 2 drops of water-dropping UV curing resin, covering with a cover glass, slightly pressing, placing the sealed slice at a position 8nm away from an ultraviolet lamp, and irradiating for 10 seconds at the wavelength of 315nm to finish curing.

2) Neutral gum method: soaking the glass slide in 75% ethanol for 1 minute, then in 85% ethanol for 1 minute, in 95% ethanol for 1 minute, in anhydrous ethanol for 1 minute, in a second anhydrous ethanol for 1 minute, in the first xylene for 1 minute, in the second xylene for 1 minute, in the third xylene for 1 minute. Finally, 2 drops of neutral gum are added dropwise, covered with a cover slip and lightly pressed, and naturally dried or oven-dried.

As a result, after the aqueous UV resin is used for curing, the background is bright and clear, the nucleus structure is clear, the cell membrane boundary contrast is high, the color is not distorted, and the dyeing solution is not dissolved and stained. The effect of the sealing piece is the same as that of the neutral gum, but the background is obviously brighter, and the transmittance is superior to that of the neutral gum in the sense of sense.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.