1. An electrophysiology patch-clamp perfusion device, comprising:

the anti-skidding support device comprises a fixed support rod (1), wherein a bearing base (2) with an anti-skidding effect is fixedly mounted at the lower end of the fixed support rod (1), a limiting hole groove (3) is formed in the outer surface of the left side of the fixed support rod (2), and a fixing frame (11) with a fixed supporting effect is arranged at the joint of the fixed support rod (1) and the bearing base (2);

the sleeve rod (4) is arranged outside the fixed support rod (1), the left end of the sleeve rod (4) is provided with a limiting bolt (5) connected with the fixed support rod (1), and the right surface of the sleeve rod (4) is provided with an anti-falling groove (18);

the injection pipe (6) is arranged at the right end of the sleeve rod (4) in a penetrating mode, a liquid inlet dustproof plug (15) for sealing a liquid inlet is arranged outside the injection pipe (6), an adjusting assembly (7) located above the sleeve rod (4) is arranged at the upper end of the injection pipe (6), scale marks (8) are arranged on the right side of the outside of the injection pipe (6), and a sealing gasket (17) is fixedly adhered to the top of the injection pipe (6);

the injection head base (9) is arranged on the lower side of the interior of the injection pipe (6), a circulating pipe (10) is arranged at the lower end of the injection head base (9), a supporting frame (12) is arranged outside the lower end of the circulating pipe (10), a limiting block (13) is arranged inside the right end of the supporting frame (12), a limiting spring (14) connected with the supporting frame (12) is arranged at the right end of the limiting block (13), the scale marks (8) are evenly arranged outside the right end of the injection pipe (6), and the injection pipe (6) and the injection head base (9) are connected in a threaded mode.

2. The electrophysiology patch-clamp perfusion apparatus of claim 1, wherein: the fixing frame (11) is arranged at an equal angle relative to the central point of the fixed supporting rod (1), and the fixed supporting rod (1), the fixing frame (11) and the bearing base (2) are all welded to form an integrated structure.

3. The electrophysiology patch-clamp perfusion apparatus of claim 1, wherein: the fixed support rod (1) and the bearing base (2) are vertically arranged.

4. The electrophysiology patch-clamp perfusion apparatus of claim 1, wherein: the limiting hole groove (3) is uniformly formed in the left side of the fixed supporting rod (1), and the fixed supporting rod (1) and the sleeve rod (4) adopt a nested sliding structure.

5. The electrophysiology patch-clamp perfusion apparatus of claim 1, wherein: the limiting bolt (5) penetrates through the loop bar (4) and is connected with the fixed supporting rod (1) in a threaded mode through the limiting hole groove (3).

6. The electrophysiology patch-clamp perfusion apparatus of claim 1, wherein: the adjusting assembly (7) further comprises a box body (701), a first adjusting rod (702), a piston block (703), an adjusting disc (704), a second adjusting rod (705) and a driving motor (706);

the box body (701) is fixedly arranged at the upper end of the injection tube (6) and is connected with the loop bar, and the upper surface of the box body (701) is provided with a fixing mechanism (16);

the first adjusting rod (702) is arranged inside the box body (701), and a groove-shaped structure is reserved on the upper surface of the box body (701);

the piston block (703) is arranged at the lower end of the first adjusting rod (702), the piston block (703) is attached to the inner wall of the injection tube (6), and the cross section of the piston block (703) is rectangular;

an adjustment dial (704) provided outside an upper end of the first adjustment lever (702);

a second adjustment lever (705) provided at the left end of the adjustment disc (704);

and a driving motor (706) provided at a rear end of the second adjustment lever (705).

7. The electrophysiology patch-clamp perfusion apparatus of claim 6, wherein: the first adjusting rod (702) is connected with the adjusting disc (704) in a threaded mode, and the adjusting disc (704) is connected with the second adjusting rod (705) in a meshed mode.

8. The electrophysiology patch-clamp perfusion apparatus of claim 6, wherein: the fixing mechanism (16) further comprises a pressing plate (1601), a positioning bump (1602), an installation through hole (1603), an anti-falling spring (1604), an anti-falling clamping block (1605) and a pulling rod (1606);

a pressing plate (1601) which is arranged on the upper surface of the box body (701) in a bonding mode;

a positioning bump (1602) which is positioned on the lower surface of the pressing plate (1601) and is integrally arranged with the positioning bump (1602) and the pressing plate (1601);

an installation through hole (1603) which is positioned on the right end surface of the pressing plate (1601) with the vertical cross section in an L-shaped structure;

the anti-falling spring (1604) is positioned at the upper end in the installation through hole (1603), and the right end of the anti-falling spring (1604) is fixedly connected with the pressing plate (1601);

the right side surface of the anti-falling clamping block (1605) is fixedly connected with the left end of the anti-falling spring (1604);

and the pulling rod (1606) is positioned below the anti-falling spring (1604), and the pulling rod (1606) and the anti-falling fixture block (1605) are integrally arranged.

9. The electrophysiology patch-clamp perfusion apparatus of claim 8, wherein: the positioning bump (1602) is arranged corresponding to the groove-shaped structure on the upper surface of the box body (701), and the lower surface of the box body (701) is attached to the upper surface of the sealing gasket (17).

10. The electrophysiology patch-clamp perfusion apparatus of claim 8, wherein: the pulling rod (1606) is also located the inside of installation through-hole (1603), and the right-hand member protrusion of pulling rod (1606) is in the right-hand member face of pressing panel (1601) to this bulge is annular structure, and installation through-hole (1603) corresponds the setting with the position of anticreep recess (18), and anticreep recess (18) and the connected mode of anticreep fixture block (1605) be the block connection, and the section of erectting of anticreep fixture block (1605) is quarter circular structure.

Background

The electrophysiology patch clamp is a device for measuring the surface of a cell membrane, can verify the existence of an ion channel, and needs perfusion operation when measuring the cell membrane, so the requirements on the electrophysiology patch clamp perfusion device are extremely strict, and the currently used electrophysiology patch clamp perfusion device still has some defects, such as:

the currently used electrophysiological patch clamp perfusion device has the following problems in the use process:

1. the perfusion speed can not be controlled according to the requirement in the using process, so that the use is inconvenient;

2. the device cannot be adjusted according to the inconvenience and requirements in the using process, and the use is not flexible;

3. the device can not effectively avoid organism infection in the using process, thereby affecting the safety of the device parts and having poor practical effect;

we therefore propose an electrophysiological patch clamp perfusion apparatus to solve the problems set forth above.

Disclosure of Invention

The invention aims to provide an electrophysiological patch clamp perfusion device, which solves the problems that the perfusion speed is inconvenient to control and adjust according to needs and the practical effect is poor in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: an electrophysiology patch-clamp perfusion device, comprising:

the lower end of the fixed supporting rod is fixedly provided with a bearing base with an anti-skidding effect, the outer surface of the left side of the fixed supporting rod is provided with a limiting hole groove, and a fixing frame with a fixed supporting effect is arranged at the joint of the fixed supporting rod and the bearing base;

the sleeve rod is arranged outside the fixed support rod, a limiting bolt connected with the fixed support rod is arranged at the left end of the sleeve rod, and an anti-falling groove is formed in the right surface of the sleeve rod;

the injection tube is arranged at the right end of the sleeve rod in a penetrating mode, a liquid inlet dustproof plug for sealing a liquid inlet is arranged outside the injection tube, an adjusting assembly located above the sleeve rod is arranged at the upper end of the injection tube, scale marks are arranged on the right side of the outside of the injection tube, and a sealing gasket is fixedly adhered to the top of the injection tube;

the injection head base is arranged on the lower side inside the injection tube, the lower end of the injection head base is provided with a runner tube, a support frame is arranged outside the lower end of the runner tube, a limiting block is arranged inside the right end of the support frame, a limiting spring connected with the support frame is arranged at the right end of the limiting block, the scale marks are evenly arranged outside the right end of the injection tube, and the injection tube and the injection head base are connected in a threaded mode.

By adopting the technical scheme, the perfusion speed can be favorably controlled, the device can be adjusted according to the requirement, and the practical effect of the device is improved.

As a preferred technical scheme of the invention, the fixed frame is arranged at an equal angle relative to the central point of the fixed supporting rod, and the fixed supporting rod, the fixed frame and the bearing base form an integrated structure in a welding mode.

By adopting the technical scheme, the fixed supporting rod can be stably placed on the ground or an operating platform under the action of the bearing base, and the firmness of the fixed supporting rod can be enhanced by utilizing the fixing frame.

As the preferable technical scheme of the invention, the fixed supporting rod and the bearing base are vertically arranged.

By adopting the technical scheme, the stress received by the fixed supporting rod can be reduced, and the use safety is improved.

As the preferable technical scheme of the invention, the limiting hole grooves are uniformly arranged on the left side of the fixed supporting rod, and the fixed supporting rod and the loop bar adopt a nested sliding structure.

By adopting the technical scheme, the height of the loop bar can be adjusted according to the use requirement.

As the preferred technical scheme of the invention, the limiting bolt penetrates through the loop bar and is connected with the fixed supporting bar in a threaded manner through the limiting hole groove.

By adopting the technical scheme, the screwing-up limiting bolt can ensure that the loop bar is prevented from falling by gravity, and the stability of the loop bar in the use process is improved.

As the preferred technical scheme of the invention, the adjusting assembly further comprises a box body, a first adjusting rod, a piston block, an adjusting disc, a second adjusting rod and a driving motor;

the box body is fixedly arranged at the upper end of the injection tube and is connected with the loop bar, and the upper surface of the box body is provided with a fixing mechanism;

the first adjusting rod is arranged inside the box body, and a groove-shaped structure is reserved on the upper surface of the box body;

the piston block is arranged at the lower end of the first adjusting rod, the piston block is attached to the inner wall of the injection tube, and the cross section of the piston block is rectangular;

an adjusting disc arranged outside the upper end of the first adjusting rod;

the second adjusting rod is arranged at the left end of the adjusting disc;

and the driving motor is arranged at the rear end of the second adjusting rod.

By adopting the technical scheme, the perfusion speed can be manually controlled under the action of the adjusting assembly, and the convenience in use is improved.

As a preferable technical scheme of the invention, the first adjusting rod is connected with the adjusting disc in a threaded mode, and the adjusting disc is connected with the second adjusting rod in a meshed mode.

By adopting the technical scheme, the adjusting disc is driven to rotate when the driving motor is turned on to rotate the second adjusting rod, so that the first adjusting rod in threaded connection moves up and down

As the preferred technical scheme of the invention, the fixing mechanism further comprises a pressing plate, a positioning lug, an installation through hole, an anti-falling spring, an anti-falling clamping block and a pulling rod;

a pressing plate which is arranged on the upper surface of the box body in a fitting manner;

the positioning lug is positioned on the lower surface of the pressing plate and is integrally arranged;

the installation through hole is positioned on the right end surface of the pressing plate with the vertical section in an L-shaped structure;

the anti-falling spring is positioned at the upper end inside the mounting through hole, and the right end of the anti-falling spring is fixedly connected with the pressing plate;

the right side surface of the anti-falling clamping block is fixedly connected with the left end of the anti-falling spring;

and the pulling rod is positioned below the anti-falling spring and is integrated with the anti-falling clamping block.

By adopting the technical scheme, the stability of the adjusting assembly can be ensured, the adjusting assembly is convenient to disassemble, and the injection tube is recycled and cleaned.

As a preferable technical scheme of the invention, the groove-shaped structures on the upper surfaces of the positioning lugs are correspondingly arranged, and the lower surface of the box body is attached to the upper surface of the sealing gasket.

By adopting the technical scheme, the sealing performance of the injection tube can be effectively ensured.

As a preferred technical solution of the present invention, the pulling rod is also located inside the installation through hole, and the right end of the pulling rod protrudes out of the right end face of the pressing plate, and the protruding portion is in an annular structure, the installation through hole and the anti-drop groove are correspondingly located, the anti-drop groove and the anti-drop fixture block are connected in a snap-fit manner, and the vertical cross section of the anti-drop fixture block is in a quarter-circle structure.

By adopting the technical scheme, the fixing mechanism is conveniently positioned automatically, and the use is convenient.

Compared with the prior art, the invention has the beneficial effects that: the electrophysiological patch clamp perfusion device is beneficial to controlling the perfusion speed, can be adjusted as required, and improves the practical effect of the device;

1. the adjusting disc is connected with the second adjusting rod in a meshing mode, the adjusting disc can be driven to rotate synchronously when the driving motor is started to rotate the second adjusting rod, so that the first adjusting rod in threaded connection is subjected to lifting adjustment, and the liquid can be extruded conveniently by matching the piston block and the inner wall of the injection tube in a fitting mode, and the perfusion speed can be controlled conveniently;

2. the sleeve rod is arranged on the fixed supporting rod to form a nested sliding structure, when the height of the sleeve rod is adjusted, the limit bolt in threaded connection is screwed, the sleeve rod can be prevented from automatically falling due to gravity, the limit hole groove is uniformly arranged on the left side of the outer part of the fixed supporting rod in a matched mode, height fixing can be conducted according to needs, and adjustability of the device is effectively improved;

3. the fixed supporting rod, the fixing frame and the bearing base are welded to form an integrated structure, so that the fixed supporting rod can be stably placed on the ground or an operation table under the action of the bearing base, the fixing frame is favorable for enhancing the firmness of the fixed supporting rod, the injection head base is matched with the injection tube to be connected in a threaded mode, infection caused by different organisms can be avoided, and the practical effect of the device is effectively improved;

4. according to the injection syringe, the box body is pressed through the pressing plate, the stability of the box body in the using process of the injection syringe can be guaranteed, meanwhile, the anti-falling clamping block is connected with the anti-falling groove through the clamping, the injection syringe is convenient to install and detach, the adjusting assembly is convenient to detach, the flexibility of the injection syringe is improved, and the injection syringe is convenient to recycle and clean and is convenient to use.

Drawings

FIG. 1 is a schematic front sectional view of the present invention;

FIG. 2 is a schematic side view of the cross-sectional structure of the present invention;

FIG. 3 is a schematic top view of the cross-sectional structure of the present invention;

FIG. 4 is an enlarged view of the structure at A in FIG. 2 according to the present invention;

FIG. 5 is a schematic sectional elevation view of the syringe and piston block connection of the present invention;

FIG. 6 is an enlarged view of the structure at B in FIG. 1 according to the present invention;

FIG. 7 is a schematic top sectional view of the syringe and the inlet dust plug of the present invention;

FIG. 8 is an enlarged view of the structure of FIG. 1 at C according to the present invention.

In the figure: 1. fixing the support rod; 2. a load-bearing base; 3. a limiting hole groove; 4. a loop bar; 5. a limiting bolt; 6. an injection tube; 7. an adjustment assembly; 701. a box body; 702. a first adjusting lever; 703. a piston block; 704. an adjusting disk; 705. a second adjusting lever; 706. a drive motor; 8. scale lines; 9. an injection head base; 10. a flow-through tube; 11. a fixed mount; 12. a support frame; 13. a limiting block; 14. a limiting spring; 15. a liquid inlet dustproof plug; 16. a fixing mechanism; 1601. a pressing plate; 1602. positioning the bump; 1603. mounting a through hole; 1604. an anti-drop spring; 1605. an anti-falling clamping block; 1606. pulling a rod; 17. a gasket; 18. Anticreep recess.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-8, the present invention provides a technical solution: an electrophysiology patch clamp perfusion device comprises a bearing base 2 with an anti-skidding effect and fixedly mounted at the lower end of a fixed support rod 1, a limiting hole groove 3 is formed in the outer surface of the left side of the fixed support rod 1, a fixing frame 11 with a fixing and supporting effect is arranged at the joint of the fixed support rod 1 and the bearing base 2, the fixed support rod 1 can be stably placed on the ground or an operation table under the action of the bearing base 2, and the firmness of the fixed support rod 1 can be enhanced by the fixing frame 11;

the sleeve rod 4 is arranged outside the fixed support rod 1, the left end of the sleeve rod 4 is provided with a limiting bolt 5 connected with the fixed support rod 1, and when the sleeve rod 4 is moved, the height of the device can be adjusted according to requirements;

the injection tube 6 is arranged at the right end of the loop bar 4 in a penetrating manner, a liquid inlet dustproof plug 15 convenient for liquid inlet is arranged outside the injection tube 6, an adjusting assembly 7 positioned above the loop bar 4 is arranged at the upper end of the injection tube 6, and a scale mark 8 is arranged on the right side of the outside of the injection tube 6;

the injection head base 9 is arranged at the lower side in the injection tube 6, the lower end of the injection head base 9 is provided with a circulating tube 10, the outer part of the lower end of the circulating tube 10 is provided with a support frame 12 connected with the fixed support rod 1, the inner part of the right end of the support frame 12 is provided with a limit block 13, and the right end of the limit block 13 is provided with a limit spring 14 connected with the support frame 12;

when the electrophysiological patch clamp perfusion device is used, firstly, all parts are assembled, the fixed support rod 1, the fixing frame 11 and the bearing base 2 are arranged to form an integrated structure in a welding mode, the fixed support rod 1 can be stably placed on the ground or an operation table under the action of the bearing base 2, the fixing frame 11 is favorable for enhancing the firmness of the fixed support rod 1, the fixed support rod 1 is uniformly arranged on the left side of the outer part of the fixed support rod 1 through the limiting hole grooves 3, the limiting bolts 5 in threaded connection can be screwed after the nested connecting sleeve rods 4 are moved, and the sleeve rods 4 can be prevented from automatically descending due to gravity;

then the pressing plate 1601 is sleeved outside the first adjusting rod 702, and presses the box body 701, the positioning lug 1602 is mutually clamped with the groove-shaped structure on the upper surface of the box body 701, so that the stability of the position between the pressing plate 1601 and the box body 701 can be effectively ensured, in the process of pressing down the pressing plate 1601, the right end of the loop bar 4 contacts with the anti-falling block 1605 with a quarter-circle structure vertical section, and the anti-falling block 1605 is extruded, so that the anti-falling block 1605 enters the installation through hole 1603 until the installation through hole 1603 is aligned with the anti-falling groove 18, under the action of the anti-falling spring 1604, the anti-falling fixture block 1605 is supported, so that the anti-falling fixture block 1605 is clamped into the anti-falling groove 18, the fixing of the position of the pressing plate 1601 can be completed, the fixing of the position of the box body 701 can be completed at the same time, the lower surface of the box body 701 is tightly attached to the upper surface of the sealing gasket 17, and the sealing performance of the interior of the injection tube 6 is guaranteed;

then an injection head base 9 is installed, then a liquid inlet dustproof plug 15 is opened to pour liquid into an injection tube 6, a circulation tube 10 is arranged at the lower end of the injection head base 9 to enable the liquid to be perfused automatically, after the injection head is used, the circulation tube 10 is fixed on a support frame 12, a limit spring 14 is arranged to form a clamping type sliding structure on the support frame 12 through a limit block 13 to limit the circulation tube 10 and prevent the liquid from spilling everywhere, or the perfusion speed can be manually controlled through an adjusting component 7 arranged at the upper end of the injection tube 6, the middle part of the circulation tube 10 is provided with a ripple arrangement to extend along with the pulling of a worker, a second adjusting rod 705 is arranged to be connected with an adjusting disc 704 in a meshing manner, and the adjusting disc is driven to rotate synchronously when a driving motor 706 arranged inside a box body 701 is opened to rotate the second adjusting rod 705, the matching adjusting disc 704 is in threaded connection with the first adjusting rod 702, so that the first adjusting rod 702 moves up and down, that is, the liquid in the injection tube 6 can be extruded by using the square piston block 703 fixedly mounted at the lower end, and the perfusion quantity of the liquid can be conveniently checked under the action of the scale mark 8 arranged at the right side outside the injection tube 6;

after using the completion, through pulling the pole 1606, drive anticreep fixture block 1605 and remove for anticreep fixture block 1605 breaks away from with anticreep recess 18 completely, will press platen 1601 rebound after that, it is spacing to the extrusion of box body 701 to remove press platen 1601, can take off box body 701 from the top of syringe 6, can be to 6 take out, thereby conveniently retrieve operations such as clearance, convenient to use 6.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.