1. The utility model provides a peroxidase preparation facilities and technology, includes jar body (1), crushing bucket (2), agitator (3), jar body (1) top is equipped with feed inlet (101), and jar body (1) bottom is equipped with stabilizer blade (106), and jar body (1) center is equipped with control panel (102) and observation window (105) simultaneously, its characterized in that: the jar body (1) is inside about both ends be equipped with fixed pivot (108) that are used for fixed crushing barrel (2) and agitator (3), and jar body (1) bottom runs through and is equipped with driving motor (107), and driving motor (107) output end meshing is connected with actuating lever (7) simultaneously.

2. The peroxidase producing apparatus according to claim 1, wherein: the utility model discloses a crushing tank, including jar body (1), jar body (1) top inner wall one side embedding has refrigerator (5), and refrigerator (5) bottom is equipped with one and extends to cold air duct (501) in smashing bucket (2) inner wall, guiding gutter (109) have been seted up to jar body (1) top inner wall opposite side, and guiding gutter (109) bottom is equipped with one and extends to the inside outlet pipe (110) of smashing bucket (2), set up built-in pipeline (111) in jar body (1) inner wall, and be equipped with check valve (112) in built-in pipeline (111) inboard inner wall, built-in pipeline (111) middle section embedding has a slope to set up and telescopic guide ring (6).

3. The peroxidase producing apparatus according to claim 2, wherein: the inside of the material guide ring (6) is sleeved with a telescopic ring (601), and the material guide ring (6) and the telescopic ring (601) are provided with corresponding material guide grooves (602) on the ring body.

4. The peroxidase producing apparatus according to claim 1, wherein: the method is as follows. Smash bucket (2) barrel inner wall in the embedding have and run through to the air conditioning transmission pipe (204) of smashing bucket (2) bottom, and air conditioning transmission pipe (204) top is equipped with check valve (203), and air conditioning transmission pipe (204) bottom intercommunication is equipped with connecting pipe (4) simultaneously, set up injection tank (202) of being connected with air conditioning pipe (501) on the inner wall of smashing bucket (2) top, and injection tank (202) bottom and check valve (203) communicate each other, be equipped with automatically controlled bin outlet (201) on smashing bucket (2) bottom barrel simultaneously, be equipped with filter layer (205) in smashing bucket (2) lateral wall, and be equipped with elasticity door (206) that opens and shuts on filter layer (205) outer wall.

5. The peroxidase producing apparatus according to claim 1, wherein: be equipped with cold air duct (303) in agitator (3) lateral wall, and cold air duct (303) top agitator (3) inner wall has seted up connecting tube groove (301) supporting with connecting pipe (4), and agitator (3) bottom is equipped with automatically controlled bottom door (302) simultaneously.

6. The peroxidase producing apparatus according to claim 1, wherein: the driving rod (7) penetrates through the crushing barrel (2) and the stirring barrel (3), a telescopic fluted disc (703) is arranged on the rod body of the driving rod (7), and the telescopic fluted disc (703) is located on the inner wall of the bottoms of the crushing barrel (2) and the stirring barrel (3).

7. The peroxidase preparation apparatus according to claim 6, wherein: the device is characterized in that a conveying hole (707) is formed in the driving rod (7), a crushing cutter (701) and a stirring cross rod (702) are integrally arranged on the rod body of the driving rod (7), an extraction groove (706) is formed in the driving rod (7) between the stirring cross rods (702), a lifting rod (708) capable of keeping the extraction groove (706) sealed is arranged at the bottom of the extraction groove (706), and a sealing plate (709) is integrally arranged at the top of the lifting rod (708).

8. The peroxidase preparation apparatus according to claim 7, wherein: the inner wall of the conveying hole (707) is provided with threads, the bottom of the conveying hole (707) is provided with a bearing base (710), a movable dialysis barrel (711) is arranged in the bearing base (710), the top of the dialysis barrel (711) is provided with a liquid inlet (712) with the same diameter as the conveying hole (707), and the barrel body of the dialysis barrel (711) is provided with a telescopic ring (713) which can be meshed with the inner wall of the conveying hole (707).

9. The peroxidase preparation apparatus according to claim 7, wherein: stirring horizontal pole (702) inside is seted up and is stored storehouse (717) of ammonium sulfate, and is equipped with differential mechanism (720) and blanking pivot (721) in the inner wall of storehouse (717) bottom, is equipped with one simultaneously between differential mechanism (720) and blanking pivot (721) and realizes power transmission's trace rod (722), stirring horizontal pole (702) bottom is equipped with stirring post (716) of vertical setting, and stirring post (716) inside be equipped with one with differential mechanism (720) meshed drive screw (718), stirring post (716) both sides are equipped with extend to outside stirring post (716) and with drive screw (718) meshed drive carousel (719).

10. A preparation process of peroxidase comprises the following steps:

the first step is as follows: cleaning and crushing horseradish raw material, adding clear water with volume being one time of that of horseradish, standing overnight at low temperature, and throwing out filtrate in the horseradish by using a centrifugal machine the next day.

The second step is that: adding the filtrate into a stirrer, slowly adding ammonium sulfate powder for 1-2 hr, and standing overnight in a refrigerating chamber.

The third step: sucking supernatant from the filtrate with siphon tube, freezing, centrifuging, collecting precipitate, adding distilled water, packaging into dialysis bag, mixing the two dialyzates, centrifuging, and collecting supernatant.

The fourth step: and pouring the supernatant into an ice salt bath, stirring, adding acetone (-15 ℃), centrifugally stirring, collecting precipitate, and adding a small amount of distilled water to obtain an enzyme solution.

Background

The horseradish peroxidase is widely applied in various biological fields and can be used for manufacturing immunological, clinical chemistry and analysis test paper.

However, in the prior art, no integrated equipment for processing and manufacturing peroxidase by using horseradish is available, raw materials cannot be fully utilized, raw materials are wasted, multiple processing procedure personnel are needed for smooth connection of procedures in the processing process, and personnel cost is increased.

Disclosure of Invention

In order to solve the technical problems, the invention provides a peroxidase preparation device and a peroxidase preparation process, which are used for solving the problems that raw materials are wasted and the cost of the raw materials is increased due to the fact that the raw materials cannot be fully utilized, and the personnel cost is increased due to the fact that operating personnel need to be increased due to the fact that processing procedures cannot be smoothly connected.

The invention relates to a peroxidase preparation device and a process, and aims and effects of the peroxidase preparation device and the process are achieved by the following specific technical means: the utility model provides a peroxidase preparation facilities and technology, includes a jar body, crushing barrel, agitator, jar body top is equipped with a feed inlet that leaks hopper-shaped, and jar body bottom is equipped with and is used for stable stabilizer blade, jar body top can one side runs through and is equipped with the filler, and jar body bottom can one side runs through and is equipped with the drain, jar body center is equipped with the control panel who is used for controlling internal device, and is equipped with the observation window on the jar body of control panel one side, jar body bottom runs through and is equipped with driving motor, and the fixed pivot of both ends fixedly connected with about jar internal portion, it is connected with crushing barrel to rotate in the fixed pivot of jar internal portion upper end, rotates in the fixed pivot of jar internal portion lower extreme simultaneously and is connected with the agitator, the meshing of driving motor output is connected with the actuating lever.

The novel energy-saving crushing tank is characterized in that a refrigerator is arranged on one side of the inner wall of the top of the tank body, a cold air pipe extending to the inside of the tank body is arranged at the bottom of the refrigerator, the cold air pipe is movably connected with the crushing barrel, a water guide groove is formed in the other side of the inner wall of the top of the tank body, a water outlet pipe extending to the inside of the crushing barrel is arranged at the bottom of the water guide groove, built-in pipelines are arranged in the inner walls of the two sides and the bottom of the tank body, one-way valves are arranged on the inner walls of the inner sides of the built-in pipelines, a material guide.

The inside telescopic flexible ring that has cup jointed of guide ring, and the guide ring sets up corresponding baffle box on one's body with the flexible ring.

Smash bucket ladle body top inner wall and offer the injection groove of being connected with the cold air pipe, and smash the barrel head portion ladle body and be equipped with automatically controlled bin outlet, the injection groove bottom is equipped with the check valve simultaneously, the check valve bottom is connected with the air conditioning transmission pipe that extends to crushing barrel head portion, and the terminal fixedly connected with connecting pipe of air conditioning transmission pipe, is equipped with sealed pivot on the terminal ladle body of connecting pipe simultaneously, be equipped with the filter layer in the crushing bucket lateral wall, and be equipped with the elasticity door that opens and shuts on the filter layer outer wall.

The inside connecting tube groove of agreeing with the connecting pipe of seting up in agitator ladle body top, and be equipped with automatically controlled end door in the agitator bottom inner wall, be equipped with the cold air duct in the agitator inner wall of connecting tube tank bottom portion simultaneously.

The actuating lever runs through crushing bucket and agitator, and actuating lever pole is equipped with two and smashes the flexible fluted disc that the interval is the same between bucket and the agitator, be located the flexible fluted disc embedding of pole body upper portion and smash barrel head inner wall, and be located the flexible fluted disc embedding agitator head inner wall of pole body lower part, smash and open the fluted disc groove that is equipped with the activity of flexible fluted disc in bucket and the agitator head inner wall, and open in the fluted disc groove both sides inner wall and be equipped with the tooth's socket with flexible fluted disc meshing.

The inside transportation hole of having seted up of actuating lever, and the actuating lever is located and is equipped with crushing sword on the pole body in smashing the bucket, and the actuating lever is located the pole body in the agitator and is equipped with the stirring horizontal pole simultaneously, the vertical extraction groove of having seted up on the actuating lever pole body between the stirring horizontal pole, and the extraction groove bottom is equipped with the lifter, and the lifter top is equipped with simultaneously and draws the groove and keep sealed closing plate.

Be equipped with the screw thread on the conveying hole inner wall, and the conveying hole bottom is equipped with the bearing base, swing joint has the dialysis bucket in the bearing base simultaneously, dialysis bucket top is equipped with one and keeps sealed income liquid mouth with the conveying hole, and is equipped with the expansion ring on the dialysis bucket ladle body, and the embedding has the telescopic spiro in the expansion ring simultaneously, the inside one-way filter screen of a detachable that is equipped with of dialysis bucket.

Stirring horizontal pole bottom is equipped with the stirring post of vertical setting, and stirs the inside storehouse of having seted up of horizontal pole, it is equipped with differential mechanism and blanking pivot to store in the inner wall of storehouse bottom, and is equipped with a trace that realizes power transmission between differential mechanism and the blanking pivot, stirring post inside is equipped with a rotatable drive screw, and the drive screw top is connected with differential mechanism, is equipped with the drive carousel that extends to outside the stirring post and mesh with drive screw in the stirring post inner wall simultaneously.

The preparation and process of the peroxidase are as follows:

the first step is as follows: cleaning and crushing horseradish raw material, adding clear water with volume being one time of that of horseradish, standing overnight at low temperature, and throwing out filtrate in the horseradish by using a centrifugal machine the next day.

The second step is that: adding the filtrate into a stirrer, slowly adding ammonium sulfate powder for 1-2 hr, and standing overnight in a refrigerating chamber.

The third step: sucking supernatant from the filtrate with siphon tube, freezing, centrifuging, collecting precipitate, adding distilled water, packaging into dialysis bag, mixing the two dialyzates, centrifuging, and collecting supernatant.

The fourth step: and pouring the supernatant into an ice salt bath, stirring, adding acetone, centrifugally stirring, collecting precipitate, and adding a small amount of distilled water to obtain an enzyme solution.

Has the advantages that:

(1) through being equipped with crushing bucket, utilize filter layer and the elasticity door that opens and shuts in the crushing bucket inner wall, can throw away the effect that crushing bucket was carried out separation with filtrating when crushing bucket high-speed rotation, realization filtrating and residue.

(2) Through being equipped with stirring horizontal pole and stirring post, stirring post makes the drive carousel rotatory and drive the drive screw rotation with the contact of filtrating when the drive column is rotatory to it is rotatory to drive differential mechanism and control the blanking pivot, realizes the effect at the in-process control ammonium sulfate that carries out the stirring and adds speed.

(3) Through being equipped with the dialysis bucket, utilize bearing base and expansion ring, can realize keeping the dialysis bucket stable when the actuating lever is rotatory, utilize the spiro in the expansion ring and transport hole inner wall thread engagement to make the effect that the dialysis bucket removed to the transport hole top when needing to collect the dialysis bucket.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention.

FIG. 2 is a schematic view of the overall cross-sectional structure of the present invention.

FIG. 3 is a schematic cross-sectional view of the can body of the present invention.

Fig. 4 is a schematic view of a material guiding ring according to the present invention.

FIG. 5 is a schematic view of the disassembled structure of the pulverizing barrel and the mixing barrel.

FIG. 6 is a schematic sectional view of the pulverizing barrel and the mixing barrel according to the present invention.

FIG. 7 is an enlarged view of the structure at A in FIG. 6 according to the present invention.

Fig. 8 is a schematic view of the driving rod structure of the present invention.

Fig. 9 is a partial sectional view of the driving rod of the present invention.

FIG. 10 is a schematic view of a dialysis barrel according to the present invention.

FIG. 11 is a schematic cross-sectional view of a dialysis barrel according to the present invention.

FIG. 12 is a schematic cross-sectional view of a mixing bar and mixing column of the present invention.

FIG. 13 is an enlarged view of the structure at B in FIG. 12 according to the present invention.

FIG. 14 is a schematic view of the inner wall structure of the pulverizing barrel according to the present invention.

In fig. 1-14, the correspondence between the component names and the reference numbers is:

1-tank body, 101-feed inlet, 102-control panel, 103-filling port, 104-drain outlet, 105-observation window, 106-support leg, 107-driving motor, 108-fixed rotating shaft, 109-water chute, 110-water outlet pipe, 111-built-in pipeline, 112-one-way valve, 113-guide ring opening, 2-crushing barrel, 201-electric control discharge port, 202-injection groove, 203-one-way valve, 204-cold air transmission pipe, 205-filter layer, 206-elastic opening and closing door, 3-stirring barrel, 301-connecting pipe groove, 302-electric control bottom door, 303-cold air pipe, 4-connecting pipe, 401-sealed bearing, 5-refrigerator, 501-cold air pipe, 6-guide ring, 601-telescopic ring, 602-guide chute, 7-driving rod, 701-crushing cutter, 702-stirring cross rod, 703-telescopic fluted disc, 704-fluted disc groove, 705-fluted disc, 706-extraction groove, 707-transportation hole, 708-lifting rod, 709-sealing plate, 710-bearing base, 711-dialysis barrel, 712-liquid inlet, 713-telescopic ring, 714-spiral ring, 715-unidirectional filter screen, 716-stirring column, 717-storage bin, 718-transmission screw, 719-driving turntable, 720-differential, 721-blanking rotating shaft and 722-linkage rod.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.

The first embodiment is as follows:

as shown in figures 1, 2, 3, 14: firstly, horseradish to be processed falls into a crushing barrel 2 through a feed inlet 101 at the top of a tank body 1, a control panel 102 controls a driving motor 107 to drive a driving rod 7 to rotate, at the moment, a crushing knife 701 on the rod body of the driving rod 7 crushes the horseradish, clear water is filled through a filling opening 103 in the tank body 1, at the moment, the clear water enters the crushing barrel 2 through a water guide groove 109 and a water outlet pipe 110, the proportion of the clear water to raw materials is 2:1, finally, a refrigerator 5 is controlled by the control panel 102 to refrigerate and is transmitted into the crushing barrel 2 through a cold air pipe 501, a telescopic fluted disc 703 at the upper part of the driving rod 7 is controlled by the control panel 102 to be meshed with a tooth groove 705 in the crushing barrel 2 and control a guide ring 6 to extend out, the driving motor 107 is controlled to rotate, at the moment, the crushing barrel 2 is driven by the driving rod 7 to rotate, and when the rotation speed of the crushing barrel 2 reaches a certain speed, an elastic opening and closing door 206 on the inner wall of the crushing barrel 2 is opened to throw filtrate in the crushing barrel 2 into a built-in the tank body 1 The filtrate enters the stirring barrel 2 through the material guide ring 6 in the pipeline 111, and after the filtrate is collected, the material guide ring 6 is controlled to be retracted, the electric control discharge opening 201 is controlled to be opened, and the filtrate is added, and then the residue inside the crushing barrel 2 is brushed into the built-in pipeline 111 and discharged through the sewage discharge opening 104.

Example two:

as shown in fig. 5, 6, 12 and 13: the control panel 102 controls the driving motor 107 to drive the driving rod 7 to rotate so that the stirring cross rod 702 and the stirring column 716 which are positioned inside the stirring barrel 3 stir the filtrate for two hours, the driving turntable 719 on the stirring column 715 contacts the filtrate and rotationally drives the transmission screw 718 to rotate while stirring, when the transmission screw 718 rotates, the differential 720 capable of adjusting the differential speed is driven to move, and simultaneously the differential speed drives the linkage 722 to drive the blanking rotating shaft 721 to rotate, so that the ammonium sulfate in the storage bin 717 falls into the filtrate at a required speed, 226 grams of ammonium sulfate is added into each liter of filtrate, the differential speed of the differential 720 is adjusted according to the specific gravity of the filtrate so that the same amount of ammonium sulfate can uniformly fall into the filtrate within two hours, and finally the refrigerating machine 5 is controlled to work to transmit cold air into the cold air pipe 303 through the cold air pipe 501, the cold air transmission pipe 204 and the connecting pipe 4. And the filtrate was allowed to stand the next day.

Example three:

as shown in fig. 8, 9 and 10: the height of the filtrate in the stirring barrel 3 is observed through the observation window 105, the lifting rod 708 is controlled to slowly descend through the control panel 102 so that the filtrate enters the dialysis barrel 711 in the driving rod 7 through the extraction groove 706, the lifting rod 706 stops descending when the filtrate reaches the bottom to prevent precipitates from entering the dialysis barrel 711, the lifting rod 708 is controlled to ascend back after extraction is finished so that the extraction groove 706 keeps sealed, distilled water is added into the stirring barrel according to the method, the electric control door 302 is controlled to open to flush the precipitates into the built-in pipeline 111 and discharge the precipitates through the drain outlet 104, finally, the telescopic ring 713 on the barrel body of the dialysis barrel 711 is controlled to extend out of the spiral ring 714 and control the driving motor 107 to slowly rotate, at the moment, the dialysis barrel 711 slowly ascends to the top of the transportation hole 707 under the action of threads, and a worker can take out the dialysis barrel 711 through the feed inlet 101.

Example four:

the filtrate from the dialysis vessel 711 was poured into a salt bath and stirred, 1 volume of acetone pre-cooled to-15 ℃ was added along the wall of the vessel using a fine dropping tube, left for a while, centrifuged at 4,000/min in a refrigerated centrifuge for 15 minutes, and the precipitate was discarded. The supernatant was added with 0.8 vol (based on the volume of the original filtrate) of acetone at-15 deg.C (the same procedure was used above), and after standing, the precipitate was collected by centrifugation in a refrigerated centrifuge. The precipitate was dissolved in a small amount of distilled water and dialyzed to remove acetone. An enzyme solution with an Rz value close to 1 can be obtained.

The working principle is as follows: raw materials are added into the crushing barrel 2 through the feeding hole 101, the driving rod 7 is driven to rotate through the driving motor 107, the crushing cutter 702 is used for crushing the raw materials, the equal amount of clear water is added into the crushing barrel 2 through the filling hole 103, the crushing barrel 2 can be controlled to rotate through controlling the telescopic fluted disc 703, filtrate is thrown out to the built-in pipeline 111 through centrifugal force, and the filtrate is guided into the stirring barrel 3 through the material guide ring 6.

The driving motor 107 drives the driving column 7 to rotate so that the stirring cross bar 702 and the stirring column 716 rotate, the driving turntable 719 on the stirring column 716 is in contact rotation with the filtrate so that the driving transmission screw 718 rotates and drives the differential 720 to rotate, and therefore the differential 720 controls the linkage 722 to drive the blanking rotating shaft 721 to rotate, and the ammonium sulfate in the storage bin 717 falls into the stirring barrel 3.

Filtrate can enter the dialysis barrel 711 through the extraction groove 706 by controlling the lifting rod 708 to lift, and the collected dialysis barrel 711 is lifted to the top of the transportation hole 707 by the telescopic ring 713 on the barrel body of the dialysis barrel 711 and is taken out through the feed port 101.