1. A convenient immunochromatographic assay kit comprises a base plate (4), and is characterized in that: be provided with sample pad (3) in the middle of bottom plate (4) up end, sample pad (3) periphery is provided with a plurality of NC membrane (6), NC membrane (6) upper surface is provided with detection line (8) and quality control line (7), and a plurality of NC membrane (6) periphery is provided with annular pad (5) that absorbs water, be provided with colloidal gold pad (10) between NC membrane (6) and sample pad (3), bottom plate (4) bottom face is connected with adhesion callus on the sole (11) all around, adhesion callus on the sole (11) bottom face is connected with easily tears membrane (2).

2. The convenient immunochromatographic assay kit according to claim 1, wherein: the upper surface of the bottom plate (4) is provided with an adhesive layer, and the sample pad (3), the NC membrane (6), the annular water absorption pad (5) and the colloidal gold pad (10) are all bonded on the upper surface of the bottom plate (4).

3. The convenient immunochromatographic assay kit according to claim 2, wherein: the bottom plate (4) is a rectangular PVC plate.

4. The convenient immunochromatographic assay kit according to claim 3, wherein: the sample pad (3) of bottom plate (4) upper surface is provided with six groups, and six groups of sample pads (3) are the annular setting, are provided with between adjacent sample pad (3) water blocking layer (1).

5. The convenient immunochromatographic assay kit according to claim 4, wherein: six groups of NC membranes (6) on the periphery of the sample pad (3) are correspondingly arranged, the six groups of NC membranes (6) are arranged in an annular mode, and a water blocking partition (9) is arranged between the adjacent NC membranes (6).

6. The convenient immunochromatographic assay kit according to claim 5, wherein: the detection line (8) is marked by a recombinant antigen solution with the concentration of 1.5mg/mLSARS-CoV-2, and the quality control line (7) is marked by an anti-rabbit secondary antibody solution with the concentration of 1.5 mg/mL.

7. The convenient immunochromatographic assay kit according to claim 6, wherein: one end of the colloidal gold pad (10) is arranged in the sample pad (3), the other end of the colloidal gold pad (10) is bonded on the upper surface of the NC membrane (6), one end of the annular water absorption pad (5) is bonded on the upper surface of the bottom plate (4), and the other end of the annular water absorption pad (5) is bonded on the upper surface of the NC membrane (6).

8. The convenient immunochromatographic assay kit according to claim 7, wherein: the water-blocking layer (1) is made of polyethylene water-blocking paint, and the water-blocking partition (9) is made of PET water-blocking tape.

Background

The immunoassay technology is a detection method for qualitatively or quantitatively analyzing an object to be detected by using an antibody or an antigen as an analysis reagent, and the basic principle of the immunoassay technology is the interaction between the antibody and the antigen. The immunoassay technology has the advantages of strong specificity, high sensitivity, simplicity and convenience and the like, is an important research means of modern life science, and has wide application prospect in the field of bioanalysis detection.

The immunochromatography technology is an emerging immunodetection technology based on the chromatography technology and antigen-antibody specific immune reaction. The immunochromatography technique is to take a strip-shaped fiber chromatography material as a solid phase, move a sample solution on a chromatography strip through capillary action, and simultaneously cause an object to be detected in a sample and a receptor (such as an antigen or an antibody) on the chromatography material, which is directed at the object to be detected, to generate specific and high-affinity immunoreaction.

In the chromatography process, the immune complex is enriched or trapped in a detection line of the chromatography material, while the receptor marked by the free chromogenic marker crosses a detection band, is automatically separated from the immune complex and is enriched or trapped in another area (quality control band) of the chromatography material in the subsequent chromatography process.

The immunochromatography detection technology has the advantages of intuition, rapidness, high detection efficiency, simple and convenient method, no pollution, low cost, high sensitivity, strong specificity and the like, so that the immunochromatography detection technology is widely applied to clinical diagnosis, field and family self-detection, and has great significance for saving lives.

The traditional immunochromatography detection technology has the advantages that each detection is a single result detection, theoretically, the complete considerable accuracy is not achieved, and the detection result is not high in accuracy, so that a convenient immunochromatography analysis kit is needed.

Disclosure of Invention

The invention aims to provide a convenient immunochromatography assay kit to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme:

the utility model provides a convenient type immunochromatographic assay kit, includes the bottom plate, be provided with the sample pad in the middle of the bottom plate up end, sample pad periphery is provided with a plurality of NC membranes, NC membrane upper surface is provided with detection line and quality control line, and a plurality of NC membrane periphery is provided with the annular pad that absorbs water, be provided with the colloidal gold pad between NC membrane and the sample pad, bottom plate bottom end face is connected with the adhesion callus on the sole all around, adhesion callus on the sole bottom end face is connected with easily dyestripping.

As a further scheme of the invention: the upper surface of the bottom plate is provided with a bonding layer, and the sample pad, the NC membrane, the annular water absorption pad and the colloidal gold pad are all bonded on the upper surface of the bottom plate.

As a further scheme of the invention: the bottom plate is a rectangular PVC plate.

As a further scheme of the invention: the sample pad of bottom plate upper surface is provided with six groups, and six groups of sample pads are the annular setting, are provided with the water blocking layer between the adjacent sample pad.

As a further scheme of the invention: six groups of NC membranes are correspondingly arranged on the periphery of the sample pad, the six groups of NC membranes are arranged in an annular shape, and a water blocking partition is arranged between the adjacent NC membranes.

As a further scheme of the invention: the detection line is scribed through a recombinant antigen solution with the concentration of 1.5mg/mLSARS-CoV-2, and the quality control line is scribed through an anti-rabbit secondary antibody solution with the concentration of 1.5 mg/mL.

As a further scheme of the invention: one end of the colloidal gold pad is arranged in the sample pad, the other end of the colloidal gold pad is bonded on the upper surface of the NC membrane, one end of the annular water absorption pad is bonded on the upper surface of the bottom plate, and the other end of the annular water absorption pad is bonded on the upper surface of the NC membrane.

As a further scheme of the invention: the water-blocking layer is made of polyethylene water-blocking paint, and the water-blocking partition is made of PET water-blocking tape.

Compared with the prior art, the invention has the beneficial effects that: the invention provides a convenient immunochromatographic assay kit, wherein a sample is dripped on a sample pad, the sample diffuses to the periphery along the sample pad, moves forwards through capillary action, and reacts with each other after dissolving a colloidal gold pad, when the sample moves to a region of a fixed detection line and a quality control line (antigen or antibody), a conjugate of the sample to be detected and a gold-labeled reagent is specifically combined with the gold-labeled reagent and is intercepted, the conjugate is gathered on the detection line, a color development result can be observed through naked eyes, the annular water absorption pad can avoid the continuous diffusion of the sample, and the kit has the advantages of convenience, rapidness, specificity, sensitivity, strong stability, no need of special equipment and reagents, intuitive result judgment and the like, is suitable for large-batch detection and large-area general examination, in addition, six groups of NC membranes are arranged on the sample and the periphery of the sample, the six groups of NC membranes are mutually independent, synchronous detection can be realized, has a contrast effect and one-time detection, and the detection effect is greatly improved by six results.

Drawings

FIG. 1 is a schematic top view of a convenient immunochromatographic assay kit;

FIG. 2 is a schematic diagram of a front view, partial cross-sectional structure of a convenient immunochromatographic assay kit.

In the figure: 1-water-resistant layer, 2-easy-to-tear film, 3-sample pad, 4-bottom plate, 5-annular water absorption pad, 6-NC film, 7-quality control line, 8-detection line, 9-water-resistant partition, 10-colloidal gold pad and 11-adhesion foot pad.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-2, the present invention provides a technical solution: the utility model provides a convenient type immunochromatographic assay kit, includes bottom plate 4, be provided with sample pad 3 in the middle of the 4 up end of bottom plate, sample pad 3 periphery is provided with a plurality of NC membranes 6, 6 upper surfaces of NC membranes are provided with detection line 8 and quality control line 7, and 6 peripheries of a plurality of NC membranes are provided with annular pad 5 that absorbs water, be provided with colloidal gold pad 10 between NC membrane 6 and the sample pad 3, 4 bottom end faces of bottom plate are connected with adhesion callus on the sole 11 all around, 11 bottom end faces of adhesion callus on the sole are connected with easily tear film 2.

The invention is used for immunochromatography analysis, specifically, a sample is dripped on a sample pad 3, the sample diffuses to the periphery along the sample pad 3, moves forwards through capillary action, dissolves a colloidal gold pad 10 and then reacts with each other, when the sample moves to the area of a fixed detection line 8 and a quality control line 7 (antigen or antibody), a conjugate of the sample to be detected and a gold-labeled reagent is specifically combined with the sample and trapped, the conjugate is gathered on the detection line 7, a color development result can be observed through naked eyes, the annular water absorption pad 5 can avoid the continuous diffusion of the sample, and the invention has the advantages of convenience, rapidness, specificity, sensitivity, strong stability, no need of special equipment and reagents, intuitive result judgment and the like, and is suitable for large-scale detection and large-area general inspection.

The upper surface of the base plate 4 is provided with an adhesive layer, the sample pad 3, the NC membrane 6, the annular water absorption pad 5 and the colloidal gold pad 10 are all bonded on the upper surface of the base plate 4, the base plate 4 is a rectangular PVC plate, the sample pad 3 on the upper surface of the base plate 4 is provided with six groups, the six groups of sample pads 3 are arranged in an annular mode, a water blocking layer 1 is arranged between the adjacent sample pads 3, the NC membrane 6 on the periphery of the sample pad 3 is correspondingly provided with six groups, the six groups of NC membranes 6 are arranged in an annular mode, and a water blocking partition 9 is arranged between the adjacent NC membranes 6.

The sample and the NC films around the sample are provided with six groups, the six groups of NC films are mutually independent, synchronous detection can be realized, the contrast effect is realized, one-time detection is realized, six results are obtained, and the detection effect is greatly improved.

The detection line 8 is scribed through a recombinant antigen solution with the concentration of 1.5mg/mLSARS-CoV-2, and the quality control line 7 is scribed through an anti-rabbit secondary antibody solution with the concentration of 1.5mg/mL, so that the detection sensitivity is higher.

One end of the colloidal gold pad 10 is arranged in the sample pad 3, the other end of the colloidal gold pad 10 is bonded on the upper surface of the NC membrane 6, one end of the annular water absorption pad 5 is bonded on the upper surface of the bottom plate 4, the other end of the annular water absorption pad 5 is bonded on the upper surface of the NC membrane 6, the water-blocking layer 1 is made of polyethylene water-blocking paint, and the water-blocking partition 9 is made of a PET water-blocking tape.

The structures of the whole analysis kit are mutually independent, the preparation is convenient, the six groups of NC membranes are mutually independent, the mutual water blocking effect is good, the results are not influenced mutually, and the detection structure is accurate.

It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.