1. The liver-protecting health-care wine rich in organic selenium is characterized by comprising the following raw material components in parts by mass:

1000 portions of organic selenium-rich sorghum

Organic selenium-rich wheat 250-375 parts

10-15 parts of saccharifying agent

6-9 parts of a leavening agent.

2. The organic selenium-rich liver-protecting and health-care wine as claimed in claim 1, wherein the organic selenium-rich sorghum is non-transgenic organic selenium-rich sorghum containing organic selenium in an amount of not less than 1 mg/Kg.

3. The organic selenium-rich liver-protecting health-care wine as claimed in claim 2, wherein the non-transgenic organic selenium-rich sorghum and organic selenium-rich wheat is sprayed with an organic selenium-rich nutrient to crop leaf surfaces at least twice during planting.

4. The organic selenium-rich liver-protecting health-care wine as claimed in claim 3, wherein the organic selenium-rich nutrient comprises 20-30 parts by weight of selenium-containing inorganic compound and 1-2 parts by weight of phosphate.

5. The organic selenium-rich liver-protecting and health-care wine as claimed in claim 4, wherein the organic selenium element contained in the organic selenium-rich liver-protecting and health-care wine is one or more organic substances selected from organic selenium-rich sorghum and organic selenium-rich wheat.

6. The organic selenium-rich liver-protecting health-care wine as claimed in claim 1, wherein the saccharifying agent comprises red yeast rice and the fermenting agent comprises selenium-rich yeast.

7. The process for preparing a liver-protecting and health-care wine rich in organic selenium according to any one of claims 1 to 6, wherein the process comprises the steps of:

s1: weighing sorghum rich in organic selenium and wheat rich in organic selenium;

s2: respectively adding the weighed sorghum rich in organic selenium and wheat rich in organic selenium into clear water for soaking;

s3: putting the soaked sorghum rich in organic selenium and wheat rich in organic selenium into a steamer to be steamed for 2-4 h;

s4: airing the cooked sorghum rich in organic selenium and wheat rich in organic selenium to 30-34 ℃, adding a saccharifying agent, uniformly mixing, and carrying out heat preservation and saccharification to obtain saccharified mash;

s5: adding a leaven into the mash, sealing and fermenting for 72h to obtain the fermented mash;

s6: and (4) pressurizing and filtering the fermented mash to obtain the organic selenium-rich health-care wine.

8. The method for preparing the organic selenium-rich liver-protecting health-care wine as claimed in claim 7, wherein the ratio of the organic selenium-rich sorghum and the organic selenium-rich wheat in S1 is 4: 1.

9. The method for preparing liver-protecting and health-care wine rich in organic selenium according to claim 7, wherein the clear water in S2 is deep-well water with pH value of 3.5-6.5.

10. The method for preparing organic selenium-rich liver-protecting and health-caring wine according to claim 7, wherein the reducing sugar concentration of the mash is not less than 5%.

Background

Wine has a very important position in human diet life all the time, and permeates through the whole long civilization history of five thousand years of China. Throughout history, wine cannot be left until the royal public noble and the dawn people, during festive festival, at home banquet sacrifice and at relatives and relatives gathering. Proper drinking has the functions of tranquilizing, promoting sleep, ventilating, dispelling cold, relaxing muscles and tendons, promoting blood circulation, stimulating gastric juice and saliva secretion, and invigorating stomach. However, because the wine contains alcohol, the wine is easy to cause alcoholism after long-time or excessive drinking, and liver cells and brain cells are damaged; chronic alcoholism can cause alcoholic cirrhosis.

In recent years, various health-care wines are developed based on the problems, but most of the existing health-care wines are added with medicines playing a certain role in treatment, and the addition of the medicines is that the taste of the wine is easily changed and the wine culture cannot be well fitted; secondly, although the increase of the medicines has a treatment effect on one disease, the components of the medicines have influence on other medicines used in daily life.

Selenium is a trace element necessary for human bodies, has important physiological functions and is closely related to the health of human beings. In 1957, the famous scientist professor shiwa z found that selenium has a magical liver protection effect when studying cases of hepatic necrosis for a long time, the selenium element is called as 'anti-hepatic necrosis protection factor', and after long-term exploration by researchers, the main effects of selenium on human bodies are summarized as follows: enhancing immunity and delaying aging; detoxification function; protecting the liver; preventing and resisting cancer; preventing cardiovascular and cerebrovascular diseases. In addition, selenium also has effects of relieving mental depression caused by working pressure, improving sexual function, treating arthritis, diabetes, resisting radiation, and protecting retina. As said by world doctor authoritative feld microelements: "selenium is like an atomic bomb, the quantity is very small, the action and power are very large, once recognized, it will have profound effect on health".

Therefore, it is needed to provide a liver-protecting health-care wine rich in organic selenium and a preparation method thereof to solve the above problems.

Disclosure of Invention

In order to solve the problems, the invention provides organic selenium-rich liver-protecting health-care wine and a preparation method thereof.

The invention aims to provide organic selenium-rich liver-protecting health-care wine, which comprises the following raw material components in parts by weight:

1000 portions of organic selenium-rich sorghum

Organic selenium-rich wheat 250-375 parts

10-15 parts of saccharifying agent

6-9 parts of a leavening agent.

The organic selenium-rich liver-protecting health-care wine provided by the invention is also characterized in that the organic selenium-rich sorghum is non-transgenic organic selenium-rich sorghum with the selenium content of not less than 1 mg/Kg.

The organic selenium-rich health care wine provided by the invention is also characterized in that the organic selenium-rich nutrient is sprayed to the leaf surfaces of crops at least twice in the planting process of the non-transgenic organic selenium-rich sorghum and organic selenium-rich wheat.

The organic selenium-rich health care wine provided by the invention is also characterized in that the organic selenium-rich nutrient comprises 20-30 parts of selenium-containing organic compound and 1-2 parts of phosphate by weight.

The organic selenium-rich liver-protecting health-care wine provided by the invention is also characterized in that the organic selenium element contained in the organic selenium-rich liver-protecting health-care wine is one or more organic matters selected from organic selenium-rich sorghum and organic selenium-rich wheat.

The liver-protecting health-care wine rich in organic selenium provided by the invention is also characterized in that the saccharifying agent comprises red yeast rice, and the fermenting agent comprises selenium-enriched yeast.

Another object of the present invention is to provide a method for preparing a liver-protecting and health-care wine rich in organic selenium, comprising the following steps:

s1: weighing sorghum rich in organic selenium and wheat rich in organic selenium;

s2: respectively adding the weighed sorghum rich in organic selenium and wheat rich in organic selenium into clear water for soaking;

s3: putting the soaked sorghum rich in organic selenium and wheat rich in organic selenium into a steamer to be steamed for 2-4 h;

s4: airing the cooked sorghum rich in organic selenium and wheat rich in organic selenium to 30-34 ℃, adding a saccharifying agent, uniformly mixing, and carrying out heat preservation and saccharification to obtain saccharified mash;

s5: adding a leaven into the mash, sealing and fermenting for 72h to obtain the fermented mash;

s6: and (4) pressurizing and filtering the fermented mash to obtain the organic selenium-rich liver-protecting health-care wine.

The preparation method of the organic selenium-rich liver-protecting health-care wine provided by the invention is also characterized in that the ratio of the organic selenium-rich sorghum to the organic selenium-rich wheat in S1 is 4: 1.

The preparation method of the liver-protecting health-care wine rich in organic selenium provided by the invention is also characterized in that the clear water in the S2 is deep-well water with the pH value of 3.5-6.5.

The preparation method of the organic selenium-rich liver-protecting health-care wine provided by the invention is also characterized in that the concentration of reducing sugar in the mash is not less than 5%.

Advantageous effects

The organic selenium-rich liver-protecting health-care wine provided by the invention adds organic selenium element required by a human body in common wine in daily life, achieves the purpose of protecting the liver through the organic selenium element taken by the human body, is brewed by pure grains without adding medicinal components, has no effect, and is soft and tasty when being drunk.

Detailed Description

In order that the objects and advantages of the invention will be more clearly understood, the invention is further described in detail below with reference to examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention. Those skilled in the art can make insubstantial modifications and adaptations to the present invention as described above. The source, trade name and if necessary the constituents of the reagents used are indicated at the first appearance.

The organic selenium content in the sorghum and wheat rich in organic selenium used in the following examples is not less than 1.5mg/Kg, and the organic selenium content is not less than 95%.

The organic selenium-rich sorghum and organic selenium-rich wheat is sprayed with an organic selenium-rich nutrient on the leaf surfaces by using an agricultural sprayer at the initial stage of ear and flower spraying, and the nutrient is timely re-sprayed in rainy days. The preparation method of the organic selenium-rich nutrient comprises the following steps: 200 parts of natural spring water 100, 20-40 parts of fertile soil, 20-40 parts of organic fertilizer, 10-20 parts of humic acid, 10-20 parts of compound amino acid, 4-8 parts of quick-acting phosphorus-potassium fertilizer, 4-8 parts of sodium selenate and 2-4 parts of chitosan oligosaccharide.

1) Preparing 100-200 parts by weight of natural spring water and 20-40 parts by weight of fertile soil, and storing in a shading way for later use, wherein the fertile soil is taken from the nature, any pesticide and chemical fertilizer are not used, and the organic matter content exceeds 60 percent;

2) uniformly mixing 100 parts by weight of natural spring water obtained in the step 1) and 20-40 parts by weight of fertile soil in a reaction kettle;

3) adding 20-40 parts by weight of organic fertilizer into 100-200 parts by weight of the natural spring water and 20-40 parts by weight of the fertile soil uniformly mixed in the step 2), and uniformly stirring to obtain a nutrient blank, wherein the content of organic matters in the organic fertilizer is not less than 25%;

4) adding 10-20 parts by weight of humic acid or humic acid and 10-20 parts by weight of compound amino acid into the 280 parts by weight of 140-280 parts by weight of the nutrient blank prepared in the step 3), and stirring at constant speed for 60min to prepare a coarse nutrient;

5) placing 320 parts by weight of the coarse nutrient prepared in the step 4) in an environment of 40-60 ℃ for fermenting for 24 hours;

6) adding 4-8 parts by weight of potassium dihydrogen phosphate and 4-8 parts by weight of sodium selenate into 320 parts by weight of the coarse nutrient which is fermented in the step 5), and stirring at constant speed for 120min to obtain the fine nutrient.

7) Placing 336 parts by weight of the 168-336 sperm nutrient prepared in the step 6) in an environment with a pH value of 6-7 and a temperature of 30-40 ℃ for aerobic fermentation for 48 hours;

8) adding 2-4 parts by weight of chitosan oligosaccharide into 168-336 parts by weight of the essence nutrient after the aerobic fermentation in the step 7) to obtain the organic selenium-rich nutrient.

The chitosan oligosaccharide mixed in the biological nutrient can change soil flora, promote growth of beneficial microorganisms, induce disease resistance of crops and prevent and treat various diseases, sodium selenate is fully mixed in the biological nutrient, the root systems of the crops can absorb inorganic selenium when absorbing necessary nutrition, and the inorganic selenium is converted into organic selenium in the bodies of the crops, so that the crops are rich in the organic selenium and are easier to absorb by human bodies.

The selenium-enriched yeast selected in the embodiment is prepared by mixing inorganic selenium and yeast with water and fermenting, wherein the mixture ratio of fermentation liquid is 2% of inorganic selenium, 6% of yeast inoculation amount, 2% of carbohydrate, 1% of nitrogen source and the balance of water according to the weight percentage. Wherein the inorganic selenium comprises positive quadrivalent inorganic selenium salt or positive hexavalent inorganic selenium salt, and the preferred inorganic selenium salt is sodium selenite. The nitrogen source is a mixture of amino acid raw powder and urea.

Example one

S1: weighing 1000 jin of sorghum rich in organic selenium and 250 jin of wheat rich in organic selenium, and carrying out hulling treatment;

s2: respectively adding the weighed organic selenium-rich sorghum and organic selenium-rich wheat into deep well water with the pH value of 6 for soaking, wherein the ratio of grain to water is 1:3, the organic selenium-rich sorghum is soaked for 4 hours, the organic selenium-rich wheat is soaked for 6 hours, then checking whether the soaked grain can be pinched into powder by fingers and has no grain core, and if the requirements are not met, continuing soaking until the fingers can be pinched into powder and have no grain core;

s3: placing the soaked selenium-rich rice and selenium-rich corn into a steamer, steaming for 3h under the steam pressure of 0.15MPa, and maintaining the pressure for 15min after the steaming is finished; the standard of cooking is cooked but not greasy, no hearts are generated inside, and cooked but not rotten, and if the standard is not met, the cooking is continued until the standard is met;

s4: after the cooked sorghum rich in organic selenium and the wheat rich in organic selenium are dried to 32 ℃, 10 jin of saccharifying red yeast are added and mixed evenly, and the saccharified mash is obtained after heat preservation and saccharification; measuring the concentration of reducing sugar by a film reagent titration method at regular time, and carrying out the next operation when the concentration of glucose reaches 5%;

s5: adding 6.25 jin of selenium-rich yeast into warm water at 30 ℃ for heat preservation and activation for 20min, adding the activated selenium-rich yeast into the mash, controlling the temperature of the mash to be 30 ℃, sealing and fermenting for 72h, and obtaining the fermented mash;

s6: and (4) pressurizing and filtering the fermented mash to obtain the organic selenium-rich liver-protecting health-care wine.

Through detection, the total amount of organic selenium in the liver-protecting health-care wine rich in organic selenium provided in the example 1 can reach 0.4 mu g/mL, and the ratio of the organic selenium is 100%.

In order to test the health care function of the liver protection health care wine rich in organic selenium provided by the above embodiment, the following experiment is carried out:

20 rats with no obvious difference in body weight and health state were selected, and 10 rats in each group were divided into a control group and an observation group. The rats in the observation group were subjected to CCl₄The vegetable oil solution is injected subcutaneously and is injected once every 4 days, the injection dosage is changed from 0.5ml/100g to 0.3ml/g on the first day, the injection is 56 days, the injection is performed for 14 times, during the period, the rat receives mixed feed (the mixture ratio is: corn flour: 79.5%, lard: 20% and cholesterol: 0.5%) to feed the rat to form hepatic fibrosis, and the rat is subjected to intragastric administration with 30% ethanol every day, and the dosage is 0.5ml/100 g. During the feeding process of the rats in the control group, the intragastric administration of ethanol is changed into the intragastric administration of distilled water. The observed groups were then divided into group A and group B with no significant difference in weight comparison. And observing that the group A receives physiological saline for intragastric administration, and observing that the group B receives organic selenium-rich liver-protecting health-care wine for intragastric administration, wherein the steps are continuously carried out for 28 days. Liver function comparisons were performed on the above three groups of rats, and the results were as follows:

as can be seen from the above table, the ALT, AST and A/G ratios of the group B observed are closer to the normal control group and in the state of improvement, and the liver function of the group A observed is obviously deteriorated compared with the normal control group. In conclusion, the organic selenium element has good regulation effect on liver/body weight ratio and various liver indexes, can reduce the incidence rate of liver cirrhosis to a certain extent, can effectively resist liver fibrosis and liver cirrhosis caused by CCL4, and meanwhile, the A/G ratio is judged according to numerical values such as ALT, liver cell damage evaluation content AST and the like and liver function damage, the three groups of contrast have obvious difference, and P is less than 0.05. The organic selenium-rich liver protection health care wine provided in example 1 has good liver protection effect and prominent health care effect.

Example two

S1: weighing 1500 jin of sorghum rich in organic selenium and 375 jin of wheat rich in organic selenium, and carrying out hulling treatment;

s2: respectively adding the weighed sorghum rich in organic selenium and wheat rich in organic selenium into deep well water with the pH value of 5.5 for soaking, wherein the ratio of grains to water is 1:3, the selenium-rich rice is soaked for 4 hours, the selenium-rich corn is soaked for 5 hours, then, whether the soaked grains can be pinched into powder by fingers and have no grain cores is checked, and if the requirements are not met, the grains are continuously soaked until the fingers can be pinched into powder and have no grain cores;

s3: placing the soaked sorghum rich in organic selenium and wheat rich in organic selenium into a steamer, steaming for 4h under the steam pressure of 0.1MPa, and maintaining the pressure for 20min after the steaming is finished; the standard of cooking is cooked but not greasy, no hearts are generated inside, and cooked but not rotten, and if the standard is not met, the cooking is continued until the standard is met;

s4: after the cooked sorghum rich in organic selenium and the wheat rich in organic selenium are dried to 33 ℃, 15 jin of saccharifying red yeast are added to be mixed evenly, and the saccharified mash is obtained after heat preservation and saccharification; measuring the concentration of reducing sugar by a film reagent titration method at regular time, and carrying out the next operation when the concentration of glucose reaches 6%;

s5: adding 9 jin of selenium-rich yeast into warm water at 28 ℃ for heat preservation and activation for 30min, adding the activated selenium-rich yeast into the mash, controlling the temperature of the mash at 28 ℃, sealing and fermenting for 72h to obtain the mash;

s6: and (4) pressurizing and filtering the fermented mash to obtain the organic selenium-rich liver-protecting health-care wine.

Through detection, the total selenium content in the liver-protecting health-care wine rich in organic selenium provided in example 1 can reach 0.5 mug/mL.

EXAMPLE III

S1: weighing 1250 jin of sorghum rich in organic selenium and 300 jin of wheat rich in organic selenium, and carrying out hulling treatment;

s2: respectively adding the weighed sorghum rich in organic selenium and wheat rich in organic selenium into deep well water with the pH value of 4 for soaking, wherein the ratio of grains to water is 1:3, the selenium-rich rice is soaked for 4 hours, after the selenium-rich corn is soaked for 6 hours, checking whether the soaked grains can be pinched into powder by fingers and have no grain cores, and if the requirements are not met, continuously soaking until the fingers can be pinched into powder and have no grain cores;

s3: placing the soaked sorghum rich in organic selenium and wheat rich in organic selenium into a steamer, steaming for 4h under the steam pressure of 0.2MPa, and maintaining the pressure for 20min after the steaming is finished; the standard of cooking is cooked but not greasy, no hearts are generated inside, and cooked but not rotten, and if the standard is not met, the cooking is continued until the standard is met;

s4: after the cooked sorghum rich in organic selenium and the wheat rich in organic selenium are aired to 34 ℃, adding 12 jin of saccharifying red yeast, uniformly mixing, and carrying out heat preservation and saccharification to obtain saccharified mash; measuring the concentration of reducing sugar by a film reagent titration method at regular time, and carrying out the next operation when the concentration of glucose reaches 5.5%;

s5: adding 8 jin of selenium-rich yeast into warm water at 30 ℃ for heat preservation and activation for 20min, adding the activated selenium-rich yeast into the mash, controlling the temperature of the mash at 30 ℃, sealing and fermenting for 72h to obtain the mash;

s6: and (4) pressurizing and filtering the fermented mash to obtain the organic selenium-rich liver-protecting health-care wine.

Through detection, the total selenium content in the liver-protecting health-care wine rich in organic selenium provided in example 1 can reach 0.36 mug/mL.

In conclusion, the organic selenium-rich liver-protecting health-care wine provided by the invention adds organic selenium elements required by a human body in common wine in daily life, achieves the purpose of protecting the liver through the selenium elements taken in by the human body, is pure grain wine, does not add various medicinal components, has no other side effects, and has better taste.

The above description is only for the specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.