1. A preparation method of a Chinese herbal medicine composition for improving cigarette aroma quality is characterized by comprising the following steps:

extracting ginseng, rhizoma acori graminei, honey radix stemonae and fructus xanthil with ethanol, and concentrating to obtain the Chinese herbal medicine composition for improving the fragrance quality of cigarettes.

2. The method of claim 1, comprising the steps of:

A) respectively soaking Ginseng radix, rhizoma Acori Graminei, radix Stemonae preparata and fructus Xanthii in water, slicing and oven drying to obtain medicinal tablet;

B) mixing the medicinal material tablets, adding an ethanol solution, and performing reflux extraction to obtain an ethanol extract;

C) and recovering ethanol from the ethanol extract, and concentrating to obtain the Chinese herbal medicine composition for improving the aroma quality of cigarettes.

3. The method for producing according to claim 2, wherein the pharmaceutical tablet is produced by:

soaking the ginseng in clear water at the temperature of 30-50 ℃ for 10-15 minutes, taking out the ginseng, cutting the ginseng into slices with the thickness of 1-2 cm, and drying the slices in a constant-temperature drying box at the temperature of 40-50 ℃ for 40 minutes to obtain ginseng slices;

soaking the rhizoma acori graminei in clear water at the temperature of 30-50 ℃ for 10-15 minutes, taking out the rhizoma acori graminei, cutting the rhizoma acori graminei into slices with the thickness of 1-2 cm, and drying the slices in a constant-temperature drying box at the temperature of 40-50 ℃ for 40 minutes to obtain rhizoma acori graminei slices;

soaking the sessile stemona root in clear water at the temperature of 20-40 ℃ for 8-10 minutes, taking out and cutting the sessile stemona root into slices with the thickness of 1-2 cm, and drying the slices in a constant-temperature drying oven at the temperature of 40-50 ℃ for 40 minutes to obtain sessile stemona root slices;

soaking the cocklebur fruit in clear water at the temperature of 60-80 ℃ for 10-15 minutes, taking out the cocklebur fruit, cutting the cocklebur fruit into slices with the thickness of 1-2 cm, and drying the slices in a constant-temperature drying oven at the temperature of 50-60 ℃ for 40 minutes to obtain the cocklebur fruit tablets.

4. The method of claim 2, wherein the amounts of ginseng, acorus gramineus soland stemona sessilifolia and xanthium sibiricum are as follows:

1-4 parts by mass of ginseng;

1-2 parts by mass of rhizoma acori graminei;

1-2 parts by mass of honey radix stemonae;

1 part by mass of cocklebur fruit.

5. The method according to claim 4, wherein the ethanol solution is an ethanol aqueous solution having a concentration of 70 to 75% by volume.

6. The preparation method according to claim 4, wherein the mass-to-volume ratio of the feed liquid obtained by reflux extraction is 1g to 10-25 ml, the extraction temperature is 75-80 ℃, and the reflux extraction is performed for 2-3 times, 1-1.5 hours each time.

7. The method according to claim 4, wherein the ethanol is recovered and then concentrated by rotary evaporation.

8. The preparation method of the cigarette is characterized by comprising the following steps:

1) tobacco leaf feeding:

adding the Chinese herbal medicine composition prepared by the preparation method of any one of claims 1 to 7 into a tobacco leaf formula of a cigarette product;

2) preparing silk:

shredding and drying the tobacco leaves after the material adding treatment, and uniformly mixing the tobacco leaves with the blending substances of the cut stems and the cut slices;

3) rolling cigarettes:

the tobacco shreds added with the Chinese herbal medicine formula are prepared into filter tip cigarettes or non-filter tip cigarettes by using cigarette production equipment together with cigarette paper, tipping paper, filter tips and other tobacco materials.

9. The method of claim 8, wherein the method of adding the prepared herbal composition to the tobacco leaves is a spraying and dipping method.

Background

At present, global smokers are more than one billion and account for about one fourth of the world population, and China has a huge market as the country with the largest global number of smokers. China is a big country for tobacco production and consumption, the tobacco consumed each year accounts for more than 1/3 of the total sale of the world, and smokers are as high as 3 hundred million.

The new tobacco cigarette has the appearance, smoke, taste and feeling similar to those of a cigarette as an electronic product replacing the cigarette. Compared with cigarettes, the cigarette does not contain 7000 harmful chemical components in cigarette smoke, so that the cigarette is favored by more and more people. The development of novel tobacco is bound to occupy a large part of tobacco industry under the drive of harm reduction requirements, and with the increasing of harm reduction requirements, the development of low-tar and alternative tobacco is vigorous. This requires tobacco enterprises to continuously seek technical innovation to meet market demands.

In addition, electronic cigarettes appearing on the market are well received in China, but the electronic cigarettes have disadvantages and benefits for human bodies, no clear report is provided at present, and some countries in European Union have no clear instruction for selling the electronic cigarettes. Therefore, for smokers who smoke for a long time, how to reduce the harm of tobacco to the maximum extent, improve the discomfort of mouth cavity of smokers during or after smoking, increase the aroma of cigarettes, and simultaneously can keep the experience of smokers on raw tobacco is a hot problem of tobacco research at present.

Disclosure of Invention

In view of the above, the technical problem to be solved by the present invention is to provide a method for preparing a Chinese herbal medicine composition for improving cigarette aroma quality and a method for preparing a cigarette, wherein the Chinese herbal medicine composition prepared by the preparation method provided by the present invention has effects of increasing cigarette smoke aroma, reducing smoke stimulation, and improving mouth discomfort and smoke residue during and after smoking of smokers.

The invention provides a preparation method of a Chinese herbal medicine composition for improving cigarette aroma quality, which comprises the following steps:

extracting ginseng, rhizoma acori graminei, honey radix stemonae and fructus xanthil with ethanol, and concentrating to obtain the Chinese herbal medicine composition for improving the fragrance quality of cigarettes.

Preferably, the method comprises the following steps:

A) respectively soaking Ginseng radix, rhizoma Acori Graminei, radix Stemonae preparata and fructus Xanthii in water, slicing and oven drying to obtain medicinal tablet;

B) mixing the medicinal material tablets, adding an ethanol solution, and performing reflux extraction to obtain an ethanol extract;

C) and recovering ethanol from the ethanol extract, and concentrating to obtain the Chinese herbal medicine composition for improving the aroma quality of cigarettes.

Preferably, the preparation method of the medicine tablet comprises the following steps:

soaking the ginseng in clear water at the temperature of 30-50 ℃ for 10-15 minutes, taking out the ginseng, cutting the ginseng into slices with the thickness of 1-2 cm, and drying the slices in a constant-temperature drying box at the temperature of 40-50 ℃ for 40 minutes to obtain ginseng slices;

soaking the rhizoma acori graminei in clear water at the temperature of 30-50 ℃ for 10-15 minutes, taking out the rhizoma acori graminei, cutting the rhizoma acori graminei into slices with the thickness of 1-2 cm, and drying the slices in a constant-temperature drying box at the temperature of 40-50 ℃ for 40 minutes to obtain rhizoma acori graminei slices;

soaking the sessile stemona root in clear water at the temperature of 20-40 ℃ for 8-10 minutes, taking out and cutting the sessile stemona root into slices with the thickness of 1-2 cm, and drying the slices in a constant-temperature drying oven at the temperature of 40-50 ℃ for 40 minutes to obtain sessile stemona root slices;

soaking the cocklebur fruit in clear water at the temperature of 60-80 ℃ for 10-15 minutes, taking out the cocklebur fruit, cutting the cocklebur fruit into slices with the thickness of 1-2 cm, and drying the slices in a constant-temperature drying oven at the temperature of 50-60 ℃ for 40 minutes to obtain the cocklebur fruit tablets.

Preferably, the dosage of the ginseng, the grassleaf sweelflag rhizome, the honey stemona root and the siberian cocklebur fruit is as follows:

1-4 parts by mass of ginseng;

1-2 parts by mass of rhizoma acori graminei;

1-2 parts by mass of honey radix stemonae;

1 part by mass of cocklebur fruit.

Preferably, the ethanol solution is 70-75% ethanol aqueous solution by volume concentration.

Preferably, the mass volume ratio of the feed liquid extracted by refluxing is 1g to 10-25 ml, the extraction temperature is 75-80 ℃, and the refluxing extraction is carried out for 2-3 times, 1-1.5 hours each time.

Preferably, the method for concentrating after ethanol recovery is rotary evaporation.

The invention also provides a preparation method of the cigarette, which comprises the following steps:

1) tobacco leaf feeding:

adding the Chinese herbal medicine composition prepared by the preparation method into a tobacco leaf formula of a cigarette product;

2) preparing silk:

shredding and drying the tobacco leaves after the material adding treatment, and uniformly mixing the tobacco leaves with the blending substances of the cut stems and the cut slices;

3) rolling cigarettes:

the tobacco shreds added with the Chinese herbal medicine formula are prepared into filter tip cigarettes or non-filter tip cigarettes by using cigarette production equipment together with cigarette paper, tipping paper, filter tips and other tobacco materials.

Preferably, the method for adding the prepared Chinese herbal medicine composition into the tobacco leaves is a spraying and soaking method.

Compared with the prior art, the invention provides a preparation method of a Chinese herbal medicine composition for improving the cigarette aroma quality, which comprises the following steps: extracting ginseng, rhizoma acori graminei, honey radix stemonae and fructus xanthil with ethanol, and concentrating to obtain the Chinese herbal medicine composition for improving the fragrance quality of cigarettes. The invention can increase the unique flavor of the cigarette smoke, reduce the irritation of the cigarette smoke, reduce the residue of the smoke flavor after smoking while ensuring the original smoking feeling of smokers, and increase the experience of the cigarette flavor, and meanwhile, the Chinese herbal medicine in the formula can relieve the mouth discomfort of the smokers, reduce the harmful ingredients in the smoke and bring new experience to the smokers.

Detailed Description

The invention provides a preparation method of a Chinese herbal medicine composition for improving cigarette aroma quality, which comprises the following steps:

extracting ginseng, rhizoma acori graminei, honey radix stemonae and fructus xanthil with ethanol, and concentrating to obtain the Chinese herbal medicine composition for improving the fragrance quality of cigarettes.

The invention takes ginseng, grassleaf sweelflag rhizome, honey stemona root and siberian cocklebur fruit as raw materials to prepare the Chinese herbal medicine composition.

The Chinese herbal medicine composition is prepared from the following Chinese herbal medicines in parts by mass: 1-4 parts by mass of ginseng; 1-2 parts by mass of rhizoma acori graminei; 1-2 parts by mass of honey radix stemonae; 1 part by mass of cocklebur fruit.

The preparation raw materials of the Chinese herbal medicine composition for improving the cigarette fragrance quality comprise 1-4 parts by mass of ginseng, preferably 1, 2, 3, 4 or any value between 1-4 parts by mass. The Chinese medicine ginseng is sweet and slightly bitter in taste, warm in nature and mild in nature. It enters spleen, lung and heart meridians. Has the effects of tonifying qi, relieving depletion, promoting fluid production, tranquilizing and improving intelligence. The Ginseng radix alcoholic extract has Ginseng radix flavor, bitter taste first and sweet after being taken, and has effects of promoting fluid production, invigorating lung, and invigorating spleen.

The preparation raw materials of the Chinese herbal medicine composition for improving the cigarette fragrance quality also comprise 1-2 parts by mass of rhizoma acori graminei, and preferably any value between 1-2 parts by mass. The Chinese medicine grassleaved sweetflag rhizome is pungent, bitter and warm in taste. It enters heart and stomach meridians. Has the effects of inducing resuscitation, eliminating phlegm, refreshing mind and improving intelligence. The alcohol extract of rhizoma Acori Graminei has pleasant smell, and has effects of eliminating dampness, eliminating phlegm and inducing resuscitation.

The preparation raw materials of the Chinese herbal medicine composition for improving the cigarette fragrance quality also comprise 1-2 parts by mass of honey stemona root, and preferably any value between 1-2 parts by mass. The Chinese medicine honey stemona is sweet and bitter in taste and is slightly warm. It enters lung meridian. Has the functions of moistening lung, depressing qi, relieving cough and killing parasite. The extract has sweet smell, and has effects of moistening lung, descending qi, and relieving cough.

The preparation raw materials of the Chinese herbal medicine composition for improving the cigarette aroma quality also comprise 1 part by mass of fructus xanthil. The traditional Chinese medicine fructus xanthil is pungent, bitter and warm in taste. It enters lung meridian. Has effects of dispelling pathogenic wind, removing dampness, and dredging nasal orifice. Fructus Xanthii alcohol extract has aromatic smell for a long time, and can be used for treating wind-cold headache, nasosinusitis and watery nasal discharge.

The four Chinese herbal medicines have unique inherent fragrance, and after compatibility, extraction concentration and optimized proportioning, the fragrance generated by the formula is long and unique, and the four Chinese herbal medicines can bring pleasure to smokers and enhance the experience of smoking processes when being added into cigarettes according to a certain proportion.

In some embodiments of the invention, the Chinese herbal medicine composition for improving the cigarette aroma quality is prepared from the following Chinese herbal medicines in parts by mass:

3 parts of ginseng; 2 parts of rhizoma acori graminei; 1 part of radix stemonae; 1 part of cocklebur fruit.

In some embodiments of the invention, the Chinese herbal medicine composition for improving the cigarette aroma quality is prepared from the following Chinese herbal medicines in parts by mass:

3 parts of ginseng; 2 parts of rhizoma acori graminei; 2 parts of radix stemonae; 1 part of cocklebur fruit.

In some embodiments of the invention, the Chinese herbal medicine composition for improving the cigarette aroma quality is prepared from the following Chinese herbal medicines in parts by mass:

4 parts of ginseng; 2 parts of rhizoma acori graminei; 2 parts of radix stemonae; 1 part of cocklebur fruit.

In some embodiments of the invention, the Chinese herbal medicine composition for improving the cigarette aroma quality is prepared from the following Chinese herbal medicines in parts by mass:

3 parts of ginseng; 1 part of rhizoma acori graminei; 1 part of radix stemonae; 1 part of cocklebur fruit.

In some embodiments of the invention, the Chinese herbal medicine composition for improving the cigarette aroma quality is prepared from the following Chinese herbal medicines in parts by mass:

1 part of ginseng; 1 part of rhizoma acori graminei; 1 part of radix stemonae; 1 part of cocklebur fruit.

In some embodiments of the invention, the Chinese herbal medicine composition for improving the cigarette aroma quality is prepared from the following Chinese herbal medicines in parts by mass:

2 parts of ginseng; 1 part of rhizoma acori graminei; 1 part of radix stemonae; 1 part of cocklebur fruit.

In the invention, the traditional Chinese medicine composition is thick paste obtained by extracting ginseng, rhizoma acori graminei, honey radix stemonae and siberian cocklebur fruit with ethanol.

Specifically, the preparation method of the Chinese herbal medicine composition comprises the following steps:

A) respectively soaking Ginseng radix, rhizoma Acori Graminei, radix Stemonae preparata and fructus Xanthii in water, slicing and oven drying to obtain medicinal tablet;

B) mixing the medicinal material tablets, adding an ethanol solution, and performing reflux extraction to obtain an ethanol extract;

C) and recovering ethanol from the ethanol extract, and concentrating to obtain the Chinese herbal medicine composition for improving the aroma quality of cigarettes.

The invention firstly screens and cleans the medicinal materials to obtain clean medicinal materials. The method of screening and washing is not particularly limited in the present invention, and may be a method known to those skilled in the art.

Then, the medicinal materials are soaked and sliced. In the present invention, it is preferable to soak and slice ginseng, acorus gramineus soland sessile stemona root and siberian cocklebur fruit as follows:

soaking the ginseng in clear water at the temperature of 30-50 ℃ for 10-15 minutes, taking out the ginseng, cutting the ginseng into slices with the thickness of 1-2 CM, drying the slices in a constant-temperature drying box at the temperature of 40-50 ℃ for 40 minutes, and putting the slices into clean food bags for later use;

soaking the rhizoma acori graminei in clear water at the temperature of 30-50 ℃ for 10-15 minutes, taking out the rhizoma acori graminei, cutting the rhizoma acori graminei into slices with the thickness of 1-2 CM, drying the slices in a constant-temperature drying box at the temperature of 40-50 ℃ for 40 minutes, and putting the slices into clean food bags for later use;

soaking the radix stemonae officinalis in clear water at the temperature of 20-40 ℃ for 8-10 minutes, taking out, cutting into slices with the thickness of 1-2 CM, drying in a constant-temperature drying box at the temperature of 40-50 ℃ for 40 minutes, and putting into a clean food bag for later use;

soaking the cocklebur fruits in clear water at the temperature of 60-80 ℃ for 10-15 minutes, taking out the cocklebur fruits, cutting the cocklebur fruits into slices with the thickness of 1-2 CM, drying the slices in a constant-temperature drying box at the temperature of 50-60 ℃ for 40 minutes, and putting the slices into clean food bags for later use;

then weighing corresponding amount of ginseng, grassleaf sweelflag rhizome, honey radix stemonae and siberian cocklebur fruit according to the formula amount, mixing, adding ethanol water solution, soaking for a period of time, and then carrying out reflux extraction. Wherein the volume concentration of the ethanol water solution is 70-75%, and the soaking time is 30-40 minutes; the mass volume ratio of the feed liquid extracted by refluxing is 1g: 10-25 ml, the extraction temperature is 75-80 ℃, and the reflux extraction is carried out for 2-3 times, 1-1.5 hours each time. After each reflux extraction, the alcohol extracts were filtered and combined. Putting the alcohol extract into a rotary evaporator, and recovering the ethanol. And finally, concentrating to obtain thick paste, namely the Chinese herbal medicine composition for improving the aroma quality of the cigarettes. The relative density of the thick paste at 60 ℃ is 1.3-1.4.

The invention also provides a cigarette, which comprises the Chinese herbal medicine composition for improving the aroma quality of the cigarette.

Wherein the addition amount of the Chinese herbal medicine composition in the cigarette is 1-5 wt%.

In the invention, the cigarette is prepared according to the following method:

1) adding the prepared thick paste into a tobacco formula of a cigarette product to generate the best quality effect;

in the invention, the method for adding the thick paste prepared by the method into the tobacco leaves is a spraying or soaking method.

In some specific embodiments of the invention, the prepared thick paste is weighed according to 1-5% of the total weight of the tobacco leaves in the formula, dissolved by 70% ethanol and placed in a feeding barrel, and the flow, the spraying angle and the spraying area of feeding equipment are adjusted, so that the feed liquid is accurately and uniformly sprayed on tobacco flakes.

2) Shredding and baking the tobacco leaves after the tobacco leaves are added with the materials, and uniformly mixing the tobacco leaves with the blending materials of the cut stems and the thin slices.

3) Rolling the tobacco shreds with the Chinese herbal medicine formula by cigarette production equipment, and preparing into filter tip cigarette or non-filter tip cigarette with cigarette paper, tipping paper and filter tip.

It should be pointed out that the above feeding method is also applicable to the preparation process of the cut tobacco, specifically, after the tobacco flakes are processed in the preparation process of the cigarette, the tobacco leaves are cut and dried, and are uniformly mixed with the blending substances of the cut stems and the thin slices, the prepared thick paste is weighed according to 1 wt% -5 wt% of the total weight of the mixed cut tobacco, the thick paste is dissolved by 70% of ethanol and is put into a feeding barrel, and the flow, the spraying angle and the spraying area of feeding equipment are adjusted, so that the feed liquid is accurately and uniformly sprayed on the cut tobacco, and the best quality effect is achieved.

The invention can increase the unique flavor of the cigarette smoke, reduce the irritation of the cigarette smoke, reduce the residue of the smoke flavor after smoking while ensuring the original smoking feeling of smokers, and increase the experience of the cigarette flavor, and meanwhile, the Chinese herbal medicine in the formula can relieve the mouth discomfort of the smokers, reduce the harmful ingredients in the smoke and bring new experience to the smokers.

The invention relates to a Chinese herbal medicine formula for increasing the aroma of cigarette smoke, which utilizes natural Chinese herbal medicines to be extracted, concentrated, optimized in proportion and added into cigarettes after special treatment, so that the original taste and smell of the tobacco are kept, the specific aroma is increased, the stimulation of the smoke is reduced, the harm of the smoke is reduced, and the discomfort of the oral cavity and the residual smoke in the smoking process of smokers or after smoking are improved.

The invention relates to a cigarette additive which is prepared by adding natural Chinese herbal medicines into cigarettes through compatibility, extraction, concentration and optimized proportion, so that the purposes of improving the fragrance of the cigarettes, relieving the stimulation of smoke and improving the uncomfortable feeling of the oral cavity and the residual smoke in the smoking process or after smoking of smokers are achieved.

For further understanding of the present invention, the following examples are provided to illustrate the preparation method of the herbal composition for improving the aroma quality of cigarette and the preparation method of a cigarette, and the scope of the present invention is not limited by the following examples.

Example 1

1. The raw material formula is as follows:

3 parts of ginseng; 2 parts of rhizoma acori graminei; 1 part of radix stemonae; 1 part of cocklebur fruit.

2. The preparation method comprises the following steps:

(1) screening and cleaning the medicinal materials to obtain clean medicinal materials.

(2) Soaking the ginseng in clear water at the temperature of 30-50 ℃ for 10-15 minutes, taking out the ginseng, cutting the ginseng into slices with the thickness of 1-2 CM, drying the slices in a constant-temperature drying box at the temperature of 40-50 ℃ for 40 minutes, and putting the slices into clean food bags for later use;

soaking the rhizoma acori graminei in clear water at the temperature of 30-50 ℃ for 10-15 minutes, taking out the rhizoma acori graminei, cutting the rhizoma acori graminei into slices with the thickness of 1-2 CM, drying the slices in a constant-temperature drying box at the temperature of 40-50 ℃ for 40 minutes, and putting the slices into clean food bags for later use;

soaking the radix stemonae officinalis in clear water at the temperature of 20-40 ℃ for 8-10 minutes, taking out, cutting into slices with the thickness of 1-2 CM, drying in a constant-temperature drying box at the temperature of 40-50 ℃ for 40 minutes, and putting into a clean food bag for later use;

soaking the cocklebur fruits in clear water at the temperature of 60-80 ℃ for 10-15 minutes, taking out the cocklebur fruits, cutting the cocklebur fruits into slices with the thickness of 1-2 CM, drying the slices in a constant-temperature drying box at the temperature of 50-60 ℃ for 40 minutes, and putting the slices into clean food bags for later use;

(3) according to the formula amount, weighing corresponding amount of ginseng, grassleaf sweelflag rhizome, honey radix stemonae and siberian cocklebur fruit, mixing, adding ethanol water solution, soaking for a period of time, and then carrying out reflux extraction. Wherein the volume concentration of the ethanol water solution is 70%, and the soaking time is 30-40 minutes; the material-liquid ratio of reflux extraction is 1:10, the extraction temperature is 75-80 ℃, and reflux extraction is carried out for 3 times, 1 hour each time. After each reflux extraction, the alcohol extracts were filtered and combined. Putting the alcohol extract into a rotary evaporator, and recovering the ethanol. Concentrating into soft extract, and refrigerating at 4 deg.C for use. The relative density of the thick paste at 60 ℃ is 1.3-1.4.

(4) Tobacco leaf feeding, namely weighing the prepared thick paste according to 2 percent of the total weight of tobacco leaves in a formula of the tobacco leaves of a cigarette product, dissolving the thick paste with 70 percent ethanol, putting the dissolved thick paste into a feeding barrel, and adjusting the flow, the spraying angle and the spraying area of feeding equipment to ensure that the feed liquid is accurately and uniformly sprayed on tobacco leaves to generate the best quality effect;

2) shredding and baking the tobacco leaves after the tobacco leaves are added with the materials, and uniformly mixing the tobacco leaves with the blending materials of the cut stems and the thin slices.

3) Rolling the tobacco shreds with the Chinese herbal medicine formula by cigarette production equipment, and preparing into filter tip cigarette or non-filter tip cigarette with cigarette paper, tipping paper and filter tip.

Example 2

1. The raw material formula is as follows:

3 parts of ginseng; 2 parts of rhizoma acori graminei; 2 parts of radix stemonae; 1 part of cocklebur fruit.

2. The preparation method comprises the following steps:

same as example 1

Example 3

1. The raw material formula is as follows:

4 parts of ginseng; 2 parts of rhizoma acori graminei; 2 parts of radix stemonae; 1 part of cocklebur fruit.

2. The preparation method is the same as example 1.

Example 4

1. The raw material formula is as follows:

3 parts of ginseng; 1 part of rhizoma acori graminei; 1 part of radix stemonae; 1 part of cocklebur fruit.

2. The preparation method is the same as example 1.

Example 5

1. The raw material formula is as follows:

1 part of ginseng; 1 part of rhizoma acori graminei; 1 part of radix stemonae; 1 part of cocklebur fruit.

2. The preparation method is the same as example 1.

Example 6

1. The raw material formula is as follows:

2 parts of ginseng; 1 part of rhizoma acori graminei; 1 part of radix stemonae; 1 part of cocklebur fruit.

2. The preparation method is the same as example 1.

Comparative example 1

1. The raw material formula is as follows:

3 parts of ginseng; 2 parts of rhizoma acori graminei; 1 part of radix stemonae.

2. The preparation method is the same as example 1.

Comparative example 2

1. The raw material formula is as follows:

3 parts of ginseng; 2 parts of rhizoma acori graminei.

2. The preparation method is the same as example 1.

Comparative example 3

1. The raw material formula is as follows:

3 parts of ginseng; 1 part of radix stemonae.

2. The preparation method is the same as example 1.

Comparative example 4

1. The raw material formula is as follows:

3 parts of ginseng; 1 part of cocklebur fruit.

2. The preparation method is the same as example 1.

Experimental example 1 comparative study of different mixture ratio of extract samples on smoked rat

1 materials of the experiment

1.1 Experimental animals

Healthy male SD rats, 84, with a body mass of 180- "200 g", purchased from Yinshi laboratory animals GmbH, Catharan city, and provided with a certification number: SCXK (Ji) -2018-.

1.2 drugs and reagents

Ginseng, grassleaf sweelflag rhizome, sessile stemona root, siberian cocklebur fruit (Jilin national security pharmaceutical industry Co., Ltd., 20180201), Changbai mountain white tobacco (Jilin tobacco industry Co., Ltd.), TNF-alpha (batch number: 20200924), IL-6 (batch number: 20200924) and IL-1 beta (batch number: 20200924) kit (purchased from Nanjing institute of Biotechnology Co., Ltd.).

1.3 Experimental instruments

An electronic balance (Fuzhou Huazhi instruments Co., Ltd.), a JA2003B electronic analytical balance (Shanghai Yuping instruments Co., Ltd.), a pulverizer (Beijing Zhongxing Weiwei instruments Co., Ltd.), a WP-UP-III-40 precision type ultra water purifier (Sichuan Watter science and technology development Co., Ltd.), a 4 ℃ low temperature centrifuge (Cerrtrifuge 5810R), a constant temperature incubator (EYEL4 SLI-700, Shanghai Erlang instruments Co., Ltd.), a MultiskanFC type microplate reader (Shanghai Saishi Feishi Shishashi).

2 method of experiment

2.1 preparation of the drug

Preparation of the extract: taking ginseng, rhizoma acori graminei, radix stemonae preparata and fructus xanthii (composition 1, mass ratio is 3:2:1: 1-embodiment 1); ginseng, grassleaf sweelflag rhizome and sessile stemona root (composition 2, mass ratio of 3:2: 1-comparative example 1); ginseng, acorus gramineus solander (composition 3, mass ratio 3: 2-comparative example 2); ginseng, radix stemonae sieboldii (composition 4, mass ratio 3: 1-comparative example 3); ginseng, fructus xanthil (composition 5, mass ratio 3: 1-comparative example 4). A thick paste was prepared according to the preparation method of example 1, and stored at 4 ℃ for future use.

Preparing an alcohol extract cigarette sample: weighing the raw tobacco, respectively weighing the thick paste according to 3% of the weight of each raw tobacco, dissolving with ethanol, spraying on the surface of the raw tobacco, drying at low temperature, and preparing the product.

2.2 grouping and administration

Grouping: 84 healthy male SD rats were randomly divided into blank, raw tobacco, composition 1, composition 2, composition 3, composition 4, composition 5, 12 per group.

Animal treatment: except for the blank group, the rats in other groups respectively receive corresponding smoking in a self-made smoking bin every day, 10 rats are smoked each time, and the smoking time is 30 min. For 4 consecutive weeks.

2.3 index detection

2.3.1 change of cough and asthma frequency of rats, after 30min of the last experiment, the change of cough frequency of rats within 5min is observed and the cough frequency of each group of rats is recorded.

2.3.2 phlegm elimination experiment: after the last experiment, 6 rats were taken from each group, 10mL/kg of IP 5% phenol red solution was added, the rats were sacrificed after 30min, the trachea segment was separated, the thyroid cartilage was taken to the trachea branch, cut into pieces, and placed in 4mL of 5% NaHCO₃Soaking in the solution in a test tube for 4h, centrifuging at 3000r/min for 15min, collecting supernatant, placing at 546nm, and measuring absorbance (A).

2.3.3 sample Collection: after the remaining 6 rats in each group were anesthetized, the neck hair was removed, the neck skin was cut open, the muscle layer was separated bluntly, the trachea was fully exposed, the right main bronchus was ligated with surgical suture, a "Y" incision was cut in the trachea near the front of the mandible, a 5mL syringe with a 16 gauge gastric lavage needle was inserted, 3mL of 1 × PBS buffer was slowly injected, after standing for 10-20s, the chest was gently pressed clockwise, the buffer was slowly withdrawn, placed in a 10mL centrifuge tube, the procedure was repeated 3 times with a recovery rate greater than 80%, the collected alveolar lavage fluid was centrifuged at 3000r/min for 15min and stored at-20 ℃ for future use.

2.3.4 rat alveolar lavage TNF- α, IL-6, IL-1 β assay: the alveolar lavage fluid collected at 2.3.3 is used for detecting the contents of TNF-alpha, IL-6 and IL-1 beta according to the kit instructions.

2.4 statistical methods

Analyzing the data by using SPSS 20.0 statistical software, and measuring the dataThe comparison adopts t test, and the difference P is less than 0.05, which has statistical significance, namely obvious difference; p < 0.01 is significant difference.

3. Results

3.1 Effect of different ratios of extract samples on rat cough and asthma frequency

Compared with the original tobacco group, the cough and asthma times of the rats of the compositions 1-4 are reduced, wherein the cough and asthma times of the composition 1 are obviously reduced and have statistical significance, and the results are shown in the table 1.

TABLE 1 Effect of different composition samples on the number of cough and asthma in rats

Note: compared with the raw tobacco group,^*P＜0.05。

3.2 Effect of different composition samples on the amount of phenol Red excreted by rats

Compared with the raw tobacco group, the composition 1-5 rats all have reduced phenol red excretion, wherein the composition 1 has obvious reduction of the phenol red excretion and has statistical significance, and the results are shown in a table 2.

TABLE 2 Effect of different composition samples on the amount of phenol Red excreted by rats

Note: compared with the raw tobacco group,^*P＜0.01。

3.3 Effect of samples of different compositions on TNF-alpha, IL-6, IL-1 beta in rat alveolar lavage fluid

Compared with the raw tobacco group, the rat alveolar lavage fluid of each group has reduced contents of TNF-alpha, IL-6 and IL-1 beta, wherein the content of TNF-alpha in the composition 1 is obviously reduced compared with the raw tobacco group, and the result is shown in Table 3.

TABLE 3 Effect of different composition samples on TNF-alpha, IL-6, IL-1 beta content in rat alveolar lavage fluid

Note: compared with the raw tobacco group,^*P＜0.05。

discussion 4

The long-time smoke inhalation or the smoke exposure can cause inflammatory reaction on rat airway epithelium, mucus thrombus is formed in bronchus, inflammatory cells in alveolar lavage fluid are increased, sputum is a pathological product formed by abnormal metabolism of water, dampness and body fluid in vivo and stagnation in vivo, and the sputum plays an important role in the occurrence and development of respiratory diseases, so that the discharge of the respiratory sputum is promoted to be beneficial to the recovery of the respiratory diseases. Prolonged smoking can result in profuse sputum and cough. After the phenol red is injected into the abdominal cavity of a rat and absorbed by the abdominal cavity, part of the phenol red is secreted into an airway by a bronchial mucus gland, and the quantity of the phenol red is increased when the bronchial secretion is increased by a medicament with the phlegm eliminating effect. Therefore, the secretion of phenol red reflects the action of eliminating phlegm. The experimental result shows that the cigarette added with the traditional Chinese medicine components can improve the cough frequency and the phenol red secretion of the rat in long-term exposed smoke to different degrees and reduce the contents of TNF-alpha, IL-6 and IL-1 beta in the rat lung. Particularly, in 5 compositions in the experiment, the content of cough frequency, phlegm eliminating, inflammatory factor content and the like of the composition 1 (ginseng, grassleaf sweelflag rhizome, sessile stemona root and siberian cocklebur fruit) is obviously reduced compared with those of the other 4 compositions, which shows that the proportion of each component in the composition 1 is better than that of the other 4 compositions.

This example was performed with different compositions and the effect of the comparative 5 compositions on reducing the number of coughs, eliminating phlegm, reducing inflammatory factors was observed. The results show that the composition 1 has more advantages in cough frequency, phlegm elimination and inflammatory factor reduction compared with other 4 groups.

Experimental example 2

1 materials of the experiment

1.1 Experimental animals

Healthy male SD rats, 60, with a body mass of 180- "200 g", purchased from Yinshi laboratory animals GmbH, Catharan city, and provided with a certification number: SCXK (Ji) -2018-.

1.2 drugs and reagents

Ginseng, grassleaf sweelflag rhizome, sessile stemona root, siberian cocklebur fruit (Jilin national security pharmaceutical industry Co., Ltd., 20180201), Changbai mountain white tobacco (Jilin tobacco industry Co., Ltd.), dexamethasone acetate tablet (sui patent pharmaceutical industry Co., Ltd., H41021038, specification 0.75 mg/tablet), SOD (batch number: 20200924), MDA (batch number: 20200924), TNF-alpha (batch number: 20200924), IL-6 (batch number: 20200924), IL-1 beta (batch number: 20200924) kit (purchased from Nanjing institute of biological research Co., Ltd.).

1.3 Experimental instruments

An electronic balance (Fuzhou Huazhi instruments Co., Ltd.), a JA2003B electronic analytical balance (Shanghai Yuping instruments Co., Ltd.), a pulverizer (Beijing Zhongxing Weiwei instruments Co., Ltd.), a WP-UP-III-40 precision type ultra water purifier (Sichuan Watter science and technology development Co., Ltd.), a 4 ℃ low temperature centrifuge (Cerrtrifuge 5810R), a constant temperature incubator (EYEL4 SLI-700, Shanghai Erlang instruments Co., Ltd.), a MultiskanFC type microplate reader (Shanghai Saishi Feishi Shishashi).

2 method of experiment

2.1 preparation of the drug

Preparation of the extract: collecting Ginseng radix, rhizoma Acori Graminei, radix Stemonae, and fructus Xanthii. Weighing the medicinal materials according to the weight ratio of 3:2:1:1 (ratio 1 group-embodiment 1), 3:2:2:1 (ratio 2 group-embodiment 2) and 1:1:1:1 (ratio 3 group-embodiment 5), preparing thick paste according to the preparation method of embodiment 1, and refrigerating at 4 ℃ for later use.

Preparing an alcohol extract cigarette sample: weighing the raw tobacco, respectively weighing the thick paste according to 3% of the weight of each raw tobacco, dissolving with ethanol, spraying on the surface of the raw tobacco, drying at low temperature, and preparing the product.

2.2 grouping and administration

Grouping: 60 healthy male SD rats are randomly divided into a blank group, a raw tobacco group, a dexamethasone acetate group, a proportion 1 group, a proportion 2 group and a proportion 3 group, wherein each group comprises 10 rats.

Animal treatment: except for the blank group, the rats in other groups respectively receive corresponding smoking in a self-made smoking bin every day, 10 rats are smoked each time, and the smoking time is 30 min. For 4 consecutive weeks.

2.3 index detection

2.3.1 change of cough and asthma frequency of rats, after 30min of the last experiment, the change of cough frequency of rats within 5min is observed and the cough frequency of each group of rats is recorded.

2.3.2 sample Collection: anesthetizing rat, collecting blood from abdominal aorta, centrifuging at 4 deg.C for 15min at 3000r/min, separating serum, and storing at-20 deg.C.

2.3.3 rat serum SDO, MDA assay: and (4) taking frozen serum and detecting the contents of SOD and MDA in the serum according to the kit instructions.

2.3.4 rat serum TNF- α, IL-6, IL-1 β assay: and (4) taking frozen serum to detect the contents of TNF-alpha, IL-6 and IL-1 beta in the serum according to the kit instructions.

2.4 statistical methods

Analyzing the data by using SPSS 20.0 statistical software, and measuring the dataThe comparison adopts t test, and the difference P is less than 0.05, which has statistical significance, namely obvious difference; p < 0.01 is significant difference.

3. Results

3.1 Effect of different ratios of extract samples on rat cough and asthma frequency

Compared with the blank group, the number of times of cough and asthma of rats in the model group is obviously increased (P is less than 0.01), and compared with the model group, the number of times of cough and asthma of rats in the dexamethasone acetate group, the proportion 1 group, the proportion 2 group and the proportion 3 group is obviously reduced (P is less than 0.05), and the results are shown in a table 4.

TABLE 4 Effect of different ratios of extract samples on the number of cough and asthma in rats

Note: in comparison with the blank set, the results,^##p is less than 0.01; in comparison with the set of models,^*P＜0.05。

3.2 Effect of different ratios of extract samples on rat serum SOD and MDA

Compared with the blank group, the serum SOD content of the rats in the model group is obviously reduced (P is less than 0.01) and the MDA content is obviously increased (P is less than 0.01), and after the administration, the serum SOD content of the rats in the dexamethasone acetate group, the rat serum SOD content of the rats in the proportion 1 group, the rat serum SOD content of the rats in the proportion 2 group and the rat serum SOD content of the rats in the proportion 3 group are obviously increased and the MDA content of the rats in the model group is obviously reduced compared with the model group, and the results are shown in a table 5.

TABLE 5 Effect of different mixture ratios of extract samples on rat serum SOD and MDA content

Note: in comparison with the blank set, the results,^##p is less than 0.01; and comparing with the model group.

3.3 Effect of different ratios of extract samples on TNF-alpha, IL-6, IL-1 beta in rat serum

Compared with the blank group, the TNF-alpha, IL-6 and IL-1 beta of the rats in the model group are obviously increased (P is less than 0.01), compared with the model group, the contents of the TNF-alpha, the IL-6 and the IL-1 beta of the rats in the dexamethasone acetate group, the proportion 1 group and the proportion 2 group are obviously reduced (P is less than 0.01) in the rats in the proportion 3 group, and the results are shown in a table 6.

TABLE 6 Effect of different ratios of extract samples on TNF-alpha, IL-6, IL-1 beta content in rat serum

Note: in comparison with the blank set, the results,^##p is less than 0.01; in comparison with the set of models,^*P＜0.05。

discussion 4

In discussing the curative effect of the medicine in treating chronic bronchitis, it is important to copy relatively accurate chronic bronchitis animal models, the invention adopts the method of smoking to induce the chronic bronchitis model rat, the method is to put the animal into the smoke chamber with proper size, introduce the smoke, take out the animal after a period of time, according to the actual demand, repeat different times and days, in order to obtain the required bronchial injury model. The method is simple and convenient to operate, can be used for various animals such as mice, dogs, monkeys and the like, has definite bronchial chronic inflammatory lesions, and is the most common method.

The smoking method induces inflammatory cells to appear under airway epithelium of mice with chronic bronchitis, mucus plugs are arranged in bronchi, protein content in alveolar lavage fluid is increased, the total number of white blood cells is increased, and the mechanism is related to over-strong oxidative stress. The experimental result shows that the extract samples with different proportions improve the levels of factors such as SOD, MDA, TNF-alpha, IL-6, IL-1 beta and the like in the serum of the rat with the chronic bronchitis model. The primary test proves that the extract samples with different proportions have certain treatment effect on the chronic bronchitis.

The invention takes extract samples with different proportions as research objects and observes the influence of the added cigarette on the smoked rat. The results show that the extraction samples with different proportions can effectively reduce the times of cough and asthma of rats, improve SOD, reduce related levels of MDA, TNF-alpha, IL-6, IL-1 beta and the like, and can reduce the harm of raw tobacco.

Experimental example 3 Effect of different mixture ratio of extract samples on ovalbumin-smoking method-induced cough variant asthma

1 materials of the experiment

1.1 Experimental animals

Healthy male SD rats, 60, with a body mass of 180- "200 g", purchased from Yinshi laboratory animals GmbH, Catharan city, and provided with a certification number: SCXK (Ji) -2018-.

1.2 drugs and reagents

Ginseng, grassleaf sweelflag rhizome, sessile stemona root, siberian cocklebur fruit (Jilin national security pharmaceutical industry Co., Ltd., 20180201), Changbai white tobacco (Jilin tobacco industry Co., Ltd.), dexamethasone acetate tablet (sui patent pharmaceutical industry Co., Ltd., H41021038, specification 0.75 mg/tablet), ovalbumin (Sigma, # SLBS6919), sodium chloride injection (Jilin Dubang pharmaceutical industry Co., Ltd., H22023069), IL-4 (batch number: 20200924), PII L-6 (batch number: 20200924), IFN-gamma (batch number: 20200924), and IgE (batch number: 20200924) kit (purchased from Nanjing Bioresearch institute of construction Co., Ltd.).

1.3 Experimental instruments

An electronic balance (Fuzhou Huazhi instrument Co., Ltd.), a JA2003B electronic analytical balance (Shanghai Yuping instrument Co., Ltd.), a JSC-202 ultrasonic atomizer (Liaoning Anshan electronic medical instrument factory), a pulverizer (Beijing Zhongxing Wei instrument Co., Ltd.), a WP-UP-III-40 precision type ultra-water purifier (Sichuan Walter science and technology development Co., Ltd.), a 4 ℃ low temperature centrifuge (Cerrtrifuge 5810R), an EYEL 4-700 constant temperature incubator (Shanghai Lang instrument Co., Ltd.), and a BC-5300Vet blood cell analyzer (Shenzhen Mirui biomedical science) MultiskanFC type enzyme reader (Shanghai Saishi Feier).

2. Experimental methods

2.1 preparation of the drug

Preparation of the extract: collecting Ginseng radix, rhizoma Acori Graminei, radix Stemonae, and fructus Xanthii. Respectively mixing the following medicinal materials in a weight ratio of 3:2:1: weighing the medicinal materials 1 (ratio 1 group-example 1), 3:2:2:1 (ratio 2 group-example 2), 1:1:1:1 (ratio 3 group-example 5), preparing into thick paste according to the preparation method of example 1, and refrigerating at 4 ℃ for later use.

Preparing an alcohol extract cigarette sample: weighing the raw tobacco, respectively weighing the thick paste according to 3% of the weight of each raw tobacco, dissolving with ethanol, spraying on the surface of the raw tobacco, drying at low temperature, and making into the final product.

2.2 grouping and model preparation

Grouping: 60 healthy male SD rats are randomly divided into a blank group, a raw tobacco group, a dexamethasone acetate group, a proportion 1 group, a proportion 2 group and a proportion 3 group, wherein each group comprises 10 rats.

Preparing a model: except for the blank group, rats of each group receive smoking of corresponding test substances in a self-made smoking cabin every day, 10 rats are used each time, 30min is used each time, after two weeks of smoking, the rats are injected with 10% egg white protein solution into abdominal cavity every other day for three times of 1mL every other day, the rats are placed in a self-made atomization box to be atomized and excited by 5% egg white protein solution, the rats are used once every day, 30min is used each time, the excitation period is 3 weeks, the smoking is continued in the excitation process, and the whole process lasts for 4 weeks^[12]The blank control group is sensitized by adopting the same dosage of normal saline for intraperitoneal injection, and is stimulated by adopting the same dosage of distilled water for atomization.

2.3 index detection

2.4.1 incubation period of inducing asthma in rat 30min after the last experiment, timing at the same time after starting atomization, and recording the time when the rat has obvious wheezing expression, namely the incubation period of inducing asthma.

2.4.2 sample Collection: anesthetizing a rat, taking blood from the abdominal aorta, adding 1mL of whole blood into an anticoagulant tube containing EDTA for standby, centrifuging the rest blood at 4 ℃ for 15min at 3000r/min, separating serum, and storing at-20 ℃ for standby. Removing neck fur, separating trachea obtusely, cutting trachea, injecting PBS buffer solution 1ml slowly into injector, standing for 10s, gently pressing chest, pumping buffer solution back, placing in 5ml centrifuge tube, repeating operation for 6 times, centrifuging alveolar lavage fluid at 3500r/min for 10min, collecting supernatant, and freezing and storing in-20 deg.C refrigerator.

2.4.3 detection of blood leukocytes, neutrophils, eosinophils: and placing the anticoagulation tube containing the whole blood under a full-automatic blood cell analyzer to automatically detect the number of leukocytes, neutrophils and eosinophils in the whole blood.

2.4.4 rat serum IL-4, I L-6, IFN-gamma determination: and (4) taking the frozen serum to detect the contents of IL-4, I L-6 and IFN-gamma in the serum according to the instruction of the kit.

2.4.5 rat alveolar lavage IgE, IFN-. gamma.assay: and (4) taking the frozen alveolar lavage fluid to detect the contents of IgE and IFN-gamma in the alveolar lavage fluid according to the instruction of the kit.

2.5 statistical methods

Analyzing the data by using SPSS 20.0 statistical software, and measuring the dataThe comparison adopts t test, and the difference is shown to be that P is less than 0.05, and the difference has statistical significance, namely has obvious difference.

3 results of the experiment

3.1 Effect of different mixture ratios of extract samples on asthma-inducing incubation period of cough variant asthma rat

Compared with a blank group, the asthma-inducing latent period of the model group rats is obviously reduced (P is less than 0.01), and the asthma-inducing latent period of the rats of each administration group is obviously prolonged (P is less than 0.01) compared with the model group.

TABLE 7 influence of different mixture ratio of extract samples on asthma-inducing latency of cough variant asthmatic rats

3.2 Effect of different mixture ratios of extract samples on WBC, EOS and NEU of cough variant asthmatic rats

Compared with the blank group, the model group rats have obviously increased blood WBC, EOS and NEU (P is less than 0.01), and compared with the model group, the rats in each group have different degrees of reduction of blood WBC, EOS and NEU (P is less than 0.05).

TABLE 8 Effect of different ratios of extract samples on WBC, EOS, NEU in cough variant asthmatic rats

Note: in comparison with the blank set, the results,^##p is less than 0.01; in comparison with the set of models,^*P＜0.05。

3.3 influence of different proportions of extract samples on the contents of IL-4, I-6 and IFN-gamma in blood serum of rats with cough variant asthma

Compared with the blank group, the contents of IL-4 and I L-6 in the serum of the rat of the model group are obviously increased (P is less than 0.01), the content of IFN-gamma is obviously reduced (P is less than 0.01), and after the administration, compared with the model group, the contents of IL-4 and I L-6(P is less than 0.01) in the serum of the rat of each administration group are obviously reduced (P is less than 0.01), and the content of IFN-gamma is obviously increased (P is less than 0.01).

TABLE 9 influence of different mixture ratios of extract samples on IL-4, I-6 and IFN-gamma contents in rat serum

Note: in comparison with the blank set, the results,^##p is less than 0.01; comparison with model group^*P＜0.05。

3.4 Effect of different mixture ratios of extract samples on content of IgE and IFN-gamma in rat alveolar lavage fluid

Compared with the blank group, the content of IgE in the alveolar lavage fluid of the rats in the model group is obviously increased, the content of IFN-gamma is obviously reduced (P is less than 0.01), and compared with the model group, the content of IgE in the alveolar lavage fluid of the rats in each administration group is obviously reduced (P is less than 0.01) and the content of IFN-gamma is obviously increased (P is less than 0.01).

TABLE 10 influence of different ratios of extract samples on the IgE and IFN-gamma content of rat alveolar lavage fluid

Note: in comparison with the blank set, the results,^##p is less than 0.01; in comparison with the set of models,^*P＜0.05。

discussion 4

Modern medicine's diagnosis of cough variant asthma focuses on blood routine, lung function determination, imaging examinations, airway inflammation assessment, etc. According to the invention, ovalbumin is adopted to copy rat cough variant asthma in combination with fumigation, and the treatment effect of extract samples with different proportions on the cough variant asthma is reflected by the change of the asthma inducing latency of the rat, the number of leukocytes, neutrophils and eosinophils in whole blood, the content of IL-4, I-6 and IFN-gamma in serum and the content of IgE and IFN-gamma in alveolar lavage fluid.

The experimental results show that: the extract samples with different proportions can obviously improve the asthma-inducing latent period of the cough variant asthma, relieve the cough symptom of the cough variant asthma rat and relieve the cough variant asthma. The change of the number of the white blood cells, the neutrophils and the eosinophils in the whole blood shows the function of the immune function to play, IL-4 and I L-6 are two marked interleukins of an interleukin family, which reflect the inflammation level of the cough variant asthma rat, and IFN-gamma has the functions of antivirus, antitumor and immune regulation. The experimental result shows that the extract samples with different proportions can reduce the WBC, EOS and NEU contents in blood, reduce the IL-4 and I-6 contents in rat serum, increase the IFN-gamma content in serum, reduce the IgE content in alveolar lavage fluid and increase the IFN-gamma content in alveolar lavage fluid. The extraction samples with different proportions can effectively reduce the lung and in-vivo inflammation level of the cough variant asthma rat.

The invention takes cough variant asthma rats as main study objects and observes the effect of the extract samples with different proportions on the rats. The experimental result shows that the extract samples with different proportions can effectively relieve the symptoms of the cough variant asthma rat, relieve the inflammation level in vivo and in the lung of the cough variant asthma rat and relieve the harm of raw smoke to the lung.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.