Algae inoculator and inoculation method

文档序号:3187 发布日期:2021-09-17 浏览:51次 中文

1. An algae inoculator, characterized in that: the utility model discloses a box, from top to bottom separate in proper order in the box and be provided with algae liquid apotheca (1), inoculation room (2) and algae liquid collection room (3), the upper portion of inoculation room (2) is provided with shower nozzle (4), the confession liquid end intercommunication of shower nozzle (4) is to the bottom of algae liquid apotheca (1), separate through horizontal punching plate (5) that set up between inoculation room (2) and the algae liquid collection room (3), inoculation culture medium (6) have been laid to punching plate (5) top, the both ends of algae liquid pipe (7) communicate respectively to the upper portion of algae liquid apotheca (1) and the lower part of algae liquid collection room (3), and still be provided with water pump (8) on algae liquid pipe (7).

2. An inoculator according to claim 1, characterised in that: the inoculation culture medium (6) is rock sample or wood.

3. An inoculator according to claim 1, characterised in that: through holes (52) are distributed on the punching plate (5), and bulges (51) are distributed on the upper surface of the punching plate (5).

4. An inoculator according to claim 1, characterised in that: an aeration device (9) is arranged in the algae liquid storage chamber (1).

5. An inoculator according to claim 1, characterised in that: a plurality of divergent spray heads (4) are distributed on the upper part of the inoculation chamber (2).

6. An inoculator according to claim 1, characterised in that: a box door (10) is arranged on the box body at one side of the inoculation chamber (2).

7. An inoculator according to claim 1, characterised in that: the side plates of one side of the inoculation chamber (2) above the inoculation culture medium (6) are all openable chamber doors (10).

8. An inoculator according to claim 1, characterised in that: the upper part of the box door (10) is hinged on the box body.

9. An inoculator according to claim 1, characterised in that: and a water stop valve (11) is also arranged on the algae liquid pipe (7).

10. An algae inoculation method is characterized in that: the method comprises the steps of arranging an algae liquid storage chamber (1), an inoculation chamber (2) and an algae liquid collection chamber (3), wherein the inoculation chamber (2) is arranged above the algae liquid collection chamber (3) and is separated from the algae liquid collection chamber (3) through a transversely arranged perforated plate (5), an inoculation culture medium (6) is laid on the perforated plate (5), a spray head (4) is arranged above the inoculation chamber (2), a liquid supply end of the spray head (4) is connected to the algae liquid storage chamber (1), the algae liquid in the algae liquid storage chamber (1) is sprayed onto the inoculation culture medium (6) in the inoculation chamber (2) to realize inoculation, then the algae liquid flows into the algae liquid collection chamber (3) through holes in the perforated plate (5), and then the algae liquid is pumped back to the algae liquid storage chamber (1) through a water pump (8).

Background

Algae inoculation is the process of inoculating algae into newly prepared culture solution/medium, and many experiments for exploring the biological weathering of algae are carried out by taking the algae from the research area and then bringing the algae back to the laboratory to be inoculated on the sample again. However, no instrument or equipment for laboratory algae inoculation experiments exists in the market at present, general algae inoculation is realized by manually inoculating algae on a sample and spraying distilled water on the sample every day, the growth period of the algae on the sample usually needs a long period of time, the manual inoculation is time-consuming, the algae inoculation is not uniform in the process of spraying the distilled water, the inoculation is not standard, and the inoculation quality is difficult to control. Therefore, there is a need for an instrument or apparatus for inoculating algae in biological weathering tests of rock, wood, and other materials in a laboratory.

Disclosure of Invention

The invention provides an algae inoculator and an inoculation method, and aims to solve the problems that no special equipment for algae inoculation exists at present, the inoculation is not standard, and the inoculation quality is difficult to control.

For realizing above-mentioned purpose, plan to adopt such an algae inoculator, the power distribution box comprises a box body, separate in proper order from top to bottom in the box and be provided with the algae liquid apotheca, inoculation room and algae liquid collection room, the upper portion of inoculation room is provided with the shower nozzle, the confession liquid end intercommunication of shower nozzle communicates the bottom to algae liquid apotheca, separate through the punching plate of horizontal setting between inoculation room and the algae liquid collection room, the inoculation culture medium has been laid to punching plate top, the both ends of algae liquid pipe communicate respectively to the upper portion of algae liquid apotheca and the lower part of algae liquid collection room, and still be provided with the water pump on the algae liquid pipe.

In the algae inoculator, the perforated plate is provided with through holes in a distributed manner, and the upper surface of the perforated plate is provided with bulges in a distributed manner, so that the through holes are prevented from being blocked due to the placement of the inoculation culture medium;

in the algae inoculator, the algae liquid storage chamber is internally provided with aeration devices in a distributed manner to promote the growth of algae;

in the algae inoculator, a plurality of divergent spray heads are distributed on the upper part of the inoculation chamber;

in the algae inoculator, a box door is arranged on a box body on one side of the inoculation chamber;

in the algae inoculator, side plates at one side of the inoculation chamber above the inoculation culture medium are all openable box doors;

in the algae inoculator, the upper part of the box door is hinged on the box body;

in the algae inoculator, a water stop valve is further arranged on the algae liquid pipe.

The invention also provides an algae inoculation method, which comprises the steps of arranging an algae liquid storage chamber, an inoculation chamber and an algae liquid collection chamber, wherein the inoculation chamber is arranged above the algae liquid collection chamber and is separated from the algae liquid collection chamber through a transversely arranged perforated plate, an inoculation culture medium is laid on the perforated plate, a spray head is arranged above the inoculation chamber, a liquid supply end of the spray head is connected to the algae liquid storage chamber, algae liquid in the algae liquid storage chamber is sprayed onto the inoculation culture medium in the inoculation chamber to realize inoculation, then the algae liquid flows into the algae liquid collection chamber through holes in the perforated plate, and then the algae liquid is pumped back to the algae liquid storage chamber through a water pump.

Compared with the prior art, the algae inoculator and the inoculation method provided by the invention are suitable for the inoculation experiments of algae such as rocks and woods, researchers can be liberated while the algae inoculation is realized, the sprayer with controllable flow rate can realize the consistency and uniformity of the inoculation amount on the surface of the sample, in addition, the algae inoculator integrates the algae culture, the algae inoculation and the algae collection, the space is saved, the recycling of algae liquid is realized, a convenient and efficient method is provided for the algae inoculation in a laboratory, the algae inoculation can be quickly realized, the inoculation is more standard and reasonable, the influence of human factors on the inoculation is reduced to the greatest extent, the algae liquid is recovered to an algae liquid storage chamber after the inoculation, the algae liquid waste is avoided, and the algae inoculator and the inoculation method are suitable for popularization and application.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a cross-sectional view of the present invention;

FIG. 3 is a schematic plan view of a perforated sheet;

figure 4 is a side view of the perforated sheet of figure 3.

Detailed Description

To make the objects, technical solutions and advantages of the present invention clearer, the present invention will be further described in detail with reference to the accompanying drawings, and it should be understood that the specific embodiments described herein are only for explaining the present invention and are not intended to limit the present invention.

Example 1

Referring to fig. 1 to 4, the embodiment provides an algae inoculator, which comprises a box body, wherein an algae liquid storage chamber 1, an inoculation chamber 2 and an algae liquid collection chamber 3 are sequentially arranged in the box body from top to bottom in a separating manner, an aeration device 9 is arranged in the algae liquid storage chamber 1 to promote algae growth, a plurality of divergent spray heads 4 are arranged on the upper portion of the inoculation chamber 2, a liquid supply end of each spray head 4 is communicated with the bottom of the algae liquid storage chamber 1, the inoculation chamber 2 and the algae liquid collection chamber 3 are separated by a transverse perforated plate 5, through holes 52 are arranged on the perforated plate 5, protrusions 51 are arranged on the upper surface of the perforated plate 5 in a distributing manner to prevent the through holes 52 from being blocked due to the placement of the inoculation culture medium 6, an inoculation culture medium 6 is laid above the perforated plate 5, the inoculation culture medium 6 is a rock sample, and two ends of an algae liquid pipe 7 are respectively communicated with the upper portion of the algae liquid storage chamber 1 and the lower portion of the algae liquid collection chamber 3, and a water stop valve 11 and a water pump 8 are also arranged on the algae liquid pipe 7.

The curb plate that inoculation room 2 one side was located 6 tops of inoculation culture medium is the chamber door 10 that can open the formula, and the upper portion of chamber door 10 articulates on the box, is convenient for place and take out inoculation culture medium 6, also is convenient for inoculation room 2's clearance and maintenance.

Example 2

Referring to fig. 1 to 4, the present embodiment provides an algae inoculator, which comprises a box body, wherein an algae liquid storage chamber 1 is arranged in the box body from top to bottom in a separating way, inoculation room 2 and algae liquid collection room 3, aeration equipment 9 has been arranged to the internal layout of algae liquid apotheca 1, promote algae growth, the upper portion distribution of inoculation room 2 is provided with a plurality of divergent shower nozzles 4, the confession liquid end of shower nozzle 4 communicates to the bottom of algae liquid apotheca 1, it separates through horizontal setting's perforated plate 5 between inoculation room 2 and the algae liquid collection room 3, through-hole 52 has been seted up to the distribution on perforated plate 5, the upper surface distribution of perforated plate 5 is provided with arch 51, prevent to make through-hole 52 block up because ligneous placing, wood (inoculation culture medium 6) have been laid to perforated plate 5 top, the both ends of algae liquid pipe 7 communicate respectively to the upper portion of algae liquid apotheca 1 and the lower part of algae liquid collection room 3, and still be provided with stagnant water valve 11 and water pump 8 on algae liquid pipe 7.

The curb plate that inoculation room 2 one side was located timber top is the chamber door 10 that can open the formula, and the upper portion of chamber door 10 articulates on the box, is convenient for place and takes out timber, also is convenient for inoculation room 2's clearance and maintenance.

Example 3

Referring to fig. 1 to 4, in the present embodiment, an algae inoculation method is provided, where an algae liquid storage chamber 1, an inoculation chamber 2, and an algae liquid collection chamber 3 are provided, an aeration device 9 is disposed in the algae liquid storage chamber 1 to promote growth of algae, the inoculation chamber 2 is disposed above the algae liquid collection chamber 3 and separated from the algae liquid collection chamber 3 by a horizontal perforated plate 5, a rock sample is laid on the perforated plate 5, a nozzle 4 is disposed above the inoculation chamber 2, a liquid supply end of the nozzle 4 is connected to the algae liquid storage chamber 1, algae liquid in the algae liquid storage chamber 1 is sprayed onto the rock sample in the inoculation chamber 2, then the algae liquid flows into the algae liquid collection chamber 3 through holes in the perforated plate 5, and is pumped back to the algae liquid storage chamber 1 by a water pump 8, and the above-mentioned steps are repeated to realize inoculation of algae on the rock sample.

The upper surface distribution of punching plate 5 is provided with arch 51, prevents to make through-hole 52 block up because placing of rock specimen, and the curb plate that inoculation room 2 one side was located the rock specimen top is the chamber door 10 that can open the formula, and the upper portion of chamber door 10 articulates on the box, is convenient for place and take out the rock specimen, also is convenient for inoculation room 2's clearance and maintenance.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.

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