1. A method for extracting the vortex worms is characterized by comprising the following steps:

s1, cleaning living bodies of the adult worms of the vortexes, drying and then crushing the living bodies, adding an organic solvent with the volume 5-7 times that of the living bodies of the vortexes for 1-2 hours, degreasing and filtering the mixture;

s2, adding water with the volume 7-9 times that of the precipitate obtained in the step S1, soaking for 4-6h, adjusting the pH, and extracting under certain pressure;

s3, adding a pH regulator into the precipitate obtained in the step S2, soaking in water for 30-50min, removing the supernatant, washing to neutrality, adding 3-4 times of water, standing at room temperature for a period of time, and collecting solid, namely insect protein, under the condition of pressure filtration.

2. The method for extracting the volunteers according to claim 1, wherein: in step S1, the organic solvent is n-hexane or benzene.

3. The method for extracting the volunteers according to claim 1, wherein: in step S2, the pressure is 100-500 MPa.

4. The method for extracting the volunteers according to claim 1, wherein: in step S2, the water temperature is 45-50 ℃.

5. The method for extracting the volunteers according to claim 1, wherein: in step S2, the pH agent is NaOH solution, and the pH is adjusted to 10-12.

6. The method for extracting the volunteers according to claim 1, wherein: in step S3, the pH agent is HCl, and the pH is adjusted to 5.0-5.5.

7. The method for extracting the volunteers according to claim 1, wherein: and in the step S3, standing for 10-12h at room temperature.

8. A product of a. volunteer protein, comprising: comprises insect protein and preservative, and the consumption of the preservative is 0.01-0.03% of the amount of the insect protein.

9. The product of the protein of the worm according to claim 8, wherein: the antiseptic is one or more of borax, glycerol, ethylene glycol, and magnesium sulfate.

10. The product of the protein of the worm according to claim 8, wherein: the antiseptic comprises 15-20% borax, 30-45% glycerol, 25-35% ethylene glycol, and 5-9% magnesium sulfate.

Background

The vortex worm is a flat animal phylum, has positive reaction to food and avoids strong light when stimulating light, and is weak light and stronger in night activities than in daytime. There are two modes of reproduction of the volunteer, of which asexual reproduction usually occurs in summer with transverse division, the division plane of which often occurs behind the pharynx. The rear end of the worm body is adhered to the bottom hook during splitting, the front end moves forwards until the worm body is broken into two halves, and then the lost halves are respectively regenerated to form 2 new individuals. Some small-sized volunteers, after several divisions, the new individuals are not immediately separated, and are connected with each other to form an insect body chain, and when the larvae grow to a certain extent, they are separated from each other to live independently. Sexual reproduction is mainly carried out in the end of autumn and in winter, but is slightly different in different regions.

The regeneration capacity of the vortex worm is particularly strong, so that the vortex worm is a good material for researching cell differentiation and dedifferentiation molecular mechanisms, animal behaviors and chromosomes, and is always an enthusiastic research field in the animal world.

Disclosure of Invention

The invention aims to provide a method for extracting the volunteer, which can realize effective extraction of biological activity and is convenient to operate.

In order to realize the purpose, the invention provides a method for extracting the worms, which comprises the following steps:

s1, cleaning living bodies of the adult worms of the vortexes, drying and then crushing the living bodies, adding an organic solvent with the volume 5-7 times that of the living bodies of the vortexes for 1-2 hours, degreasing and filtering the mixture;

s2, adding water with the volume 7-9 times that of the precipitate obtained in the step S1, soaking for 4-6h, adjusting the pH, and extracting under certain pressure;

s3, adding a pH regulator into the precipitate obtained in the step S2, soaking in water for 30-50min, removing the supernatant, washing to neutrality, adding 3-4 times of water, standing at room temperature for a period of time, and collecting solid, namely insect protein, under the condition of pressure filtration.

Preferably, in step S1, the organic solvent is n-hexane or benzene.

Preferably, in step S2, the pressure is 100-500 MPa.

Preferably, in step S2, the water temperature is 45-50 ℃.

Preferably, in step S2, the pH agent is NaOH solution, and the pH is adjusted to 10-12.

Preferably, in step S3, the pH agent is HCl and the pH is adjusted to 5.0-5.5.

Preferably, in step S3, the mixture is left standing at room temperature for 10-12 h.

A product of a protein of a Worm comprises the worm protein and a preservative in an amount of 0.01 to 0.03% of the worm protein.

Preferably, the preservative is one or more of borax, glycerol, ethylene glycol and magnesium sulfate.

Preferably, the preservative comprises 15-20% of borax, 30-45% of glycerol, 25-35% of ethylene glycol and 5-9% of magnesium sulfate.

Therefore, the method for extracting the volunteer can realize effective extraction of biological activity and is convenient to operate.

The technical solution of the present invention is further described in detail by the following examples.

Detailed Description

The technical solution of the present invention is further illustrated by the following examples.

Example one

A method for extracting the vortex worms comprises the following steps:

s1, cleaning living bodies of the adult worms of the vortexes, drying and then crushing the living bodies, adding an organic solvent with the volume 5 times that of the living bodies for 1 to 2 hours, degreasing and filtering the mixture; the organic solvent is n-hexane or benzene.

S2, adding water with the volume 7 times that of the precipitate obtained in the step S1, soaking for 5 hours, adjusting the pH, and extracting under the pressure of 200 MPa; the water temperature was 45 ℃. The pH agent is NaOH solution, and the pH is adjusted to 11.

S3, adding a pH regulator into the precipitate obtained in the step S2, soaking in water for 40min, removing the supernatant, washing to neutrality, adding 3-fold water, standing at room temperature for a period of time, and collecting solid, namely insect protein, under the condition of pressure filtration. The pH agent was HCl and the pH was adjusted to 5.0. Standing at room temperature for 11 h.

Example two

A product of a protein of a Worm comprises the worm protein and a preservative in an amount of 0.01 to 0.03% of the worm protein. The antiseptic is one or more of borax, glycerol, ethylene glycol, and magnesium sulfate.

The antiseptic comprises 15-20% borax, 30-45% glycerol, 25-35% ethylene glycol, and 5-9% magnesium sulfate.

Comparative example 1

A method for extracting the vortex worms comprises the following steps:

s1, cleaning living bodies of the adult worms of the vortexes, drying and crushing the living bodies, adding water with the volume 7 times that of the living bodies, soaking the living bodies for 5 hours, adjusting the pH value, and extracting the mixture under the pressure of 200 MPa; the water temperature was 45 ℃. The pH agent is NaOH solution, and the pH is adjusted to 11.

S2, adding a pH regulator into the precipitate obtained in the step S2, soaking in water for 40min, removing the supernatant, and collecting the solid under the condition of pressure filtration. The pH agent was HCl and the pH was adjusted to 5.0.

The insect proteins obtained in the first example and the first comparative example are respectively dissolved in ethyl acetate, and liquid quality analysis is carried out, so that the peaks obtained in the first comparative example are large in quantity and are disordered, and the peaks of the proteins obtained in the first example are simple and pure.

Therefore, the method for extracting the volunteer can realize effective extraction of biological activity and is convenient to operate.

Finally, it should be noted that: the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that: modifications and equivalents may be made to the invention without departing from the spirit and scope of the invention.