1. An apparatus for detecting an myocardial infarction protein marker, characterized in that the apparatus for detecting an myocardial infarction protein marker comprises a biosensor and a data processor connected with the biosensor; wherein, the sensing layer in the biosensor is hydrogel containing nucleic acid aptamer;

the biosensor is used for transmitting a bioelectrical signal generated by blood and the aptamer to the data processor;

the data processor is used for processing the bioelectrical signal to obtain myocardial infarction protein marker concentration information.

2. The apparatus for detecting an myocardial infarction protein marker as claimed in claim 1 wherein the thickness of the sensing layer is 1.4 mm.

3. The apparatus of detecting an aureolysin marker according to claim 1, wherein the biosensor further comprises a first circuit board and a graphene layer disposed on the first circuit board;

the sensing layer is arranged on the graphene layer;

the first circuit board is connected with the data processor;

the graphene layer is used for detecting a bioelectrical signal generated by blood and the nucleic acid aptamer and sending the bioelectrical signal to the data processor through the first circuit board.

4. The apparatus of detecting a stemmed protein marker of claim 3, wherein the graphene layer comprises a substrate and a field effect transistor disposed on the substrate;

the sensing layer is arranged on the field effect tube;

the substrate is arranged on the first circuit board;

the field effect transistor is connected with the first circuit board and used for detecting the bioelectricity signals and sending the bioelectricity signals to the data processor through the first circuit board.

5. The apparatus according to claim 4, wherein the field effect transistor comprises a first electrode pad, a second electrode pad, and a conductive channel connecting the first electrode pad and the second electrode pad;

the sensing layer is arranged on the conductive channel;

the first electrode plate, the second electrode plate and the conductive channel are all arranged on the substrate; the first electrode plate and the second electrode plate are connected with the first circuit board;

the conductive channel is made of graphene; the first electrode plate and the second electrode plate are both gold electrode plates.

6. The apparatus for detecting an myocardial infarction protein marker as claimed in claim 3, wherein the biosensor further comprises a housing;

the shell is connected with the first circuit board to form a cavity; the graphene layer, the electrode plate and the sensing layer are arranged in the cavity;

the shell is provided with a hole, the hole is located above the sensing layer, and the hole is used for inflow of blood.

7. The apparatus for detecting an myocardial infarction protein marker as claimed in claim 6, wherein the hole is formed with a hole area smaller than or equal to the surface area of the upper surface of the sensing layer.

8. The apparatus for detecting an myocardial infarction protein marker as claimed in claim 1, wherein the data processor comprises a second circuit board, and a data processing module and a display screen connected with the second circuit board;

the second circuit board is also connected with the biosensor;

the data processing module is used for processing the bioelectricity signal to obtain myocardial infarction protein marker concentration information;

the display screen is connected with the data processing module and is used for displaying the myocardial infarction protein marker concentration information.

9. The apparatus of claim 8, wherein the data processor further comprises a comparison module and an alert module;

the comparison module is connected with the data processing module and is used for judging whether the myocardial infarction protein marker concentration information is higher than a set concentration threshold value;

the warning module is connected with the comparison module and used for sending a warning signal when the result output by the comparison module is that the myocardial infarction protein marker information is higher than a set concentration threshold value.

10. The apparatus for detecting an myocardial infarction protein marker as claimed in claim 8, wherein the data processor further comprises a wireless interface and a battery module;

the second circuit board is connected with the display screen through the wireless interface;

the battery module is arranged on the second circuit board, the battery module is connected with the data processing module, and the battery module is used for supplying power to the data processing module.

Background

In the modern society, high-grade chronic cardiovascular diseases such as hypertension, angina pectoris, heart failure and myocardial infarction are becoming the primary public safety problems threatening human health. In medicine, according to the severity of the disease, the content of marker molecules such as unstable angina, stable angina and acute myocardial infarction, Cardiac troponin I (cTnI) in blood of a patient shows an abnormal rising trend, and when the content of cTnI in blood continuously exceeds 50ng/mL, sudden myocardial infarction is possible. Therefore, the method realizes the close tracking monitoring of the health state of the high-risk patient by monitoring the concentration change of the cardiovascular disease marker, and reminds the patient to seek medical advice in time in the early stage of occurrence of unforeseen myocardial infarction, acute heart failure and other events, thereby reducing the disability and fatality rate of the disease and having very important clinical medical application value.

However, the currently used myocardial infarction protein marker detection device is large in size, complex in assembly and very expensive, and is usually only equipped in large-scale comprehensive hospitals or medical detection institutions in cities. Moreover, the device has extremely high requirements on samples, professional medical staff are required to process the blood samples, and detection can be carried out after serum is obtained, so that the operation is time-consuming.

For some underdeveloped county village and town areas, because of the lack of detection equipment and operation experience, the detection of corresponding indexes of patients after the attack of diseases such as myocardial infarction and the like is often inexperienced, and the patient is delayed in illness or misdiagnosed with a high probability. Therefore, a device which is small in size, portable and simple to operate and can directly and rapidly detect the myocardial infarction marker in blood and pre-diagnose the myocardial infarction is needed.

Disclosure of Invention

The invention aims to provide a device for detecting an myocardial infarction protein marker, which is used for realizing the rapid detection of the myocardial infarction protein marker.

In order to achieve the purpose, the invention provides the following scheme:

an apparatus for detecting a myocardial infarction protein marker, comprising a biosensor and a data processor connected to the biosensor; wherein, the sensing layer in the biosensor is hydrogel containing nucleic acid aptamer;

the biosensor is used for transmitting a bioelectrical signal generated by blood and the aptamer to the data processor;

the data processor is used for processing the bioelectrical signal to obtain myocardial infarction protein marker concentration information.

Optionally, the thickness of the sensing layer is 1.4 mm.

Optionally, the biosensor further comprises a first circuit board and a graphene layer disposed on the first circuit board;

the sensing layer is arranged on the graphene layer;

the first circuit board is connected with the data processor;

the graphene layer is used for detecting a bioelectrical signal generated by blood and the nucleic acid aptamer and sending the bioelectrical signal to the data processor through the first circuit board.

Optionally, the graphene layer comprises a substrate and a field effect transistor disposed on the substrate;

the sensing layer is arranged on the field effect tube;

the substrate is arranged on the first circuit board;

the field effect transistor is connected with the first circuit board and used for detecting the bioelectricity signals and sending the bioelectricity signals to the data processor through the first circuit board.

Optionally, the field effect transistor includes a first electrode plate, a second electrode plate, and a conductive channel connecting the first electrode plate and the second electrode plate;

the sensing layer is arranged on the conductive channel;

the first electrode plate, the second electrode plate and the conductive channel are all arranged on the substrate; the first electrode plate and the second electrode plate are connected with the first circuit board;

the conductive channel is made of graphene; the first electrode plate and the second electrode plate are both gold electrode plates.

Optionally, the biosensor further comprises a housing;

the shell is connected with the first circuit board to form a cavity; the graphene layer, the electrode plate and the sensing layer are arranged in the cavity;

the shell is provided with a hole, the hole is located above the sensing layer, and the hole is used for inflow of blood.

Optionally, the hole area formed by the holes is smaller than or equal to the surface area of the upper surface of the sensing layer.

Optionally, the data processor includes a second circuit board, and a data processing module and a display screen connected to the second circuit board;

the second circuit board is also connected with the biosensor;

the data processing module is used for processing the bioelectricity signal to obtain myocardial infarction protein marker concentration information;

the display screen is connected with the data processing module and is used for displaying the myocardial infarction protein marker concentration information.

Optionally, the data processor further comprises a comparison module and an alert module;

the comparison module is connected with the data processing module and is used for judging whether the myocardial infarction protein marker concentration information is higher than a set concentration threshold value;

the warning module is connected with the comparison module and used for sending a warning signal when the result output by the comparison module is that the myocardial infarction protein marker information is higher than a set concentration threshold value.

Optionally, the data processor further comprises a wireless interface and a battery module;

the second circuit board is connected with the display screen through the wireless interface;

the battery module is arranged on the second circuit board, the battery module is connected with the data processing module, and the battery module is used for supplying power to the data processing module.

According to the specific embodiment provided by the invention, the invention discloses the following technical effects:

the device for detecting the myocardial infarction protein marker comprises a biosensor and a data processor connected with the biosensor; wherein, the sensing layer in the biosensor is hydrogel containing aptamer. The biosensor transmits a bioelectrical signal generated by the blood and the aptamer to the data processor, and the data processor processes the bioelectrical signal to obtain the myocardial infarction protein marker concentration information. The invention realizes the self-filtration of blood through the hydrogel, so that the aptamer in the hydrogel smoothly acts with the myocardial infarction protein marker in the blood to generate a bioelectricity signal, and the bioelectricity signal is processed by the data processor to obtain the concentration information of the myocardial infarction protein marker, thereby realizing the rapid detection of the myocardial infarction protein marker.

In addition, the device of the myocardial infarction protein marker formed by the biosensor and the data processor has simple structure and simple and convenient operation.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without inventive exercise.

FIG. 1 is a schematic structural diagram of a biosensor in the apparatus for detecting a cardiac infarction protein marker of the present invention;

FIG. 2 is a schematic structural diagram of the arrangement of the plug seat in the device for detecting the myocardial infarction protein marker of the present invention.

Description of the symbols:

1-nucleic acid aptamer, 2-hydrogel, 3-first circuit board, 4-first electrode plate, 5-second electrode plate, 6-conductive channel, 7-substrate, 8-shell, 9-fine copper wire, 10-conductive silver colloid, 11-welding spot electrode, 12-to-be-detected liquid, 13-PDMS gasket and 14-socket.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention aims to provide a device for detecting a myocardial infarction protein marker, which realizes express detection of the myocardial infarction protein marker in blood by a biosensor and has the advantages of simple structure and convenient operation.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

The device for detecting the myocardial infarction protein marker comprises a biosensor and a data processor connected with the biosensor; as shown in FIG. 1, the sensing layer in the biosensor is a hydrogel 2 containing an aptamer 1; in particular, the thickness of the sensing layer is 1.4 mm.

The biosensor is used for transmitting a bioelectrical signal generated by blood and the aptamer 1 to the data processor; the data processor is used for processing the bioelectrical signal to obtain myocardial infarction protein marker concentration information.

The biosensor further comprises a first circuit board 3 and a graphene layer arranged on the first circuit board 3; the sensing layer is arranged on the graphene layer; the first circuit board 3 is connected with the data processor; the graphene layer is used for detecting a bioelectrical signal generated by blood and the nucleic acid aptamer and sending the bioelectrical signal to the data processor through the first circuit board 3.

Preferably, the graphene layer comprises a substrate 7 and a field effect transistor disposed on the substrate 7; the sensing layer is arranged on the field effect tube; the substrate 7 is disposed on the first circuit board 3, and specifically, the substrate 7 is fixed on the first circuit board 3 by a double-sided tape. The field effect transistor is connected with the first circuit board and used for detecting the bioelectricity signal and sending the bioelectricity signal to the data processor through the first circuit board 3.

Further, the field effect transistor comprises a first electrode plate 4, a second electrode plate 5 and a conductive channel 6 connecting the first electrode plate 4 and the second electrode plate 5; in particular, the conductive channel 6 is disposed between the first electrode sheet 4 and the second electrode sheet 5. The sensing layer is arranged on the conductive channel 6; the first electrode plate 4, the second electrode plate 5 and the conductive channel 6 are all arranged on the substrate; and the first electrode plate 4 and the second electrode plate 5 are both connected with the first circuit board 3, in this embodiment, the first electrode plate 4 is connected with a welding spot electrode 11 on the first circuit board 3 sequentially through a thin copper wire 9 and a conductive silver adhesive 10. The second electrode slice 5 is connected with a welding spot electrode 11 on the first circuit board 3 through a thin copper wire 9 and a conductive silver adhesive 10 in sequence; the conductive channel 6 is made of graphene; the first electrode plate and the second electrode plate are both gold electrode plates. In the present embodiment, the drain electrode of the fet is the first electrode pad 4, and the source electrode is the second electrode pad 5; alternatively, the drain electrode of the field effect transistor is the second electrode plate 5, and the source electrode is the first electrode plate 4. After the field effect tube detects a biological electric signal, a first electrode plate 4 and a second electrode plate 5 of the field effect tube send drain-source current of the field effect tube to the data processor through the first circuit board 3.

Preferably, the biosensor further comprises a housing 8; the shell 8 is connected with the first circuit board 3 to form a cavity; specifically, the housing 8 is fixed on the first circuit board 3 by using a double-sided tape, and a gasket (PDMS gasket 13) is used to seal between the housing 8 and the first circuit board 3. The first electrode plate 4, the second electrode plate 5, the conductive channel 6 and the sensing layer are arranged in the cavity; the shell 8 is provided with a hole, the hole is located above the sensing layer, and the hole is used for inflow of blood. Blood is dripped on the sensing layer through the holes, so that the aim of detecting the myocardial infarction protein marker is fulfilled.

Further, the area of the hole formed by the holes is smaller than or equal to the surface area of the upper surface of the sensing layer. The shell 8 with the holes can facilitate the dripping and accurate positioning of the liquid 12 to be detected, and meanwhile, fragile conductive silver colloid and copper wires can be protected.

In this embodiment, the data processor includes a second circuit board, and a data processing module and a display screen connected to the second circuit board; the second circuit board is also connected with the biosensor; specifically, the second circuit board is connected with the first circuit board 3 in the biosensor sequentially through a plug and a lead, so that power supply and signal transmission in the biosensor are realized, the connection mode is external connection, and the second circuit board can be removed after the biosensor is used and is replaced by a new biosensor after the biosensor is out of service.

Further, the sockets 14, specifically, the sockets 13 are two xh2.54mm2P sockets on the first circuit board 3 of the biosensor, and the xh2.54mm2P sockets are also provided on the second circuit board of the data processor, the two sockets are connected by the xh2.54mm double-ended terminal line 2P, and the source interfaces on the two terminals are internally grounded.

The data processing module is used for processing the bioelectricity signal to obtain myocardial infarction protein marker concentration information; the display screen is connected with the data processing module, the display screen is used for displaying the concentration information of the myocardial infarction protein marker, and the display screen can also perform further related setting on the detection process of the myocardial infarction protein marker. Specifically, the display screen is a touch display screen.

Preferably, the data processor further comprises a comparison module and an alarm module; the comparison module is connected with the data processing module and is used for judging whether the myocardial infarction protein marker concentration information is higher than a set concentration threshold value; the warning module is connected with the comparison module and used for sending a warning signal when the result output by the comparison module is that the myocardial infarction protein marker information is higher than a set concentration threshold value. Specifically, the warning module is an indicator light, and when the concentration of the myocardial infarction protein marker is detected to be within a set concentration threshold range, the indicator light is green; when the myocardial infarction protein marker concentration is detected to be higher than a set concentration threshold value, the indicator light turns red, and an early warning effect is achieved.

Further, the data processor also comprises a wireless interface and a battery module; the second circuit board is connected with the display screen through the wireless interface, and specifically can be in wireless WIFI connection; the battery module is arranged on the second circuit board, connected with the data processing module and used for supplying power to the data processing module; the battery module is also connected with the biosensor and can supply power to the biosensor. Specifically, the battery module is 4-section 1.2V button batteries.

In a specific embodiment of the present invention, the size of the sensing layer in the biosensor is about 1 cm. When blood is dripped into the sensing layer, due to the fact that the hydrogel is of a porous structure, other substances in the blood are blocked, serum containing the myocardial infarction protein marker flows in, the myocardial infarction protein marker and the nucleic acid aptamer act, and weak current is generated. The material of the conductive channel of the field effect tube in the graphene layer is set to be graphene, and the myocardial infarction protein marker is adsorbed to the surface of the graphene by utilizing the molecular adsorption performance of the graphene, so that the conductivity between a drain electrode and a source electrode in the field effect tube is changed, the charge change of weak current is detected, and the detected weak current is transmitted to the data processor. And the data processor collects and processes weak current to obtain the concentration of the myocardial infarction protein marker, and the concentration is displayed on the display screen.

In addition, the hole on biosensor's the shell is enough big in this embodiment to improve the area of contact on blood and the sensing layer surface, and then increase the area of contact such as graphite alkene surface and myocardial infarction protein, thereby improve molecular adsorption volume, improve and detect the precision.

Compared with the prior art, the invention also has the following advantages:

(1) the biosensor provided by the invention realizes self-filtration of blood by arranging the hydrogel, prevents blood cells in the blood from forming biofouling on the surface of graphene, and ensures smooth detection, so that the direct detection of the original blood can be realized.

(2) In the device for detecting the myocardial infarction protein marker, the self-made first circuit board and the self-made second circuit board have the functions of stable output of tiny voltage and accurate collection of weak current signals, so that the portable collection of graphene field effect transistor signals can be realized without using a large power supply workstation.

(3) The device for detecting the myocardial infarction protein marker can keep more than half a year under the conventional storage condition without failure; the detection speed is high, and the detection result can be read within 5-10 minutes; can directly detect the original blood; serum can also be detected; the method can be used for detection in non-standard medical environments such as families, ambulances and the like.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.

The principles and embodiments of the present invention have been described herein using specific examples, which are provided only to help understand the method and the core concept of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.