1. Ni (OH) for non-enzymatic glucose detection₂The preparation method of the nanosheet sensor is characterized by comprising the following steps:

taking the foamed nickel as a working electrode, then adopting a three-electrode system, soaking the foamed nickel in a nickel acetate solution, and carrying out electrodeposition treatment to obtain Ni (OH) load₂Carrying out plasma treatment on the nano-sheet foamed nickel to obtain the Ni (OH) for non-enzymatic glucose detection₂A nanosheet sensor.

2. The Ni (OH) for non-enzymatic glucose testing of claim 1₂The preparation method of the nanosheet sensor is characterized in that the concentration of the nickel acetate solution is 0.01-0.2 mol/L.

3. The Ni (OH) for non-enzymatic glucose testing of claim 1₂The preparation method of the nanosheet sensor is characterized in that the electrodeposition treatment time is 100-500 s.

4. The Ni (OH) for non-enzymatic glucose testing of claim 1₂The preparation method of the nanosheet sensor is characterized in that the current density of the electrodeposition treatment is 1-30mA/cm²。

5. The Ni (OH) for non-enzymatic glucose testing of claim 1₂Nanosheet sensorThe preparation method of the sensor is characterized in that in the three-electrode system, foamed nickel is used as a working electrode, Ag/AgCl is used as a reference electrode, and Pt is used as a counter electrode.

6. The Ni (OH) for non-enzymatic glucose testing of claim 1₂The preparation method of the nanosheet sensor is characterized in that argon plasma is adopted for plasma treatment; the power of the plasma treatment is 160W-200W, and the time of the plasma treatment is 0-40 min.

7. Ni (OH) for non-enzymatic glucose assay prepared by the method of any one of claims 1 to 6₂A nanosheet sensor.

8. The Ni (OH) for non-enzymatic glucose testing of claim 7₂Application of the nanosheet sensor in detecting the concentration of glucose in blood.

9. The Ni (OH) for non-enzymatic glucose testing of claim 8₂The application of the nanosheet sensor in detecting the concentration of glucose in blood is characterized by comprising the following steps:

the Ni (OH) for non-enzymatic glucose detection₂The method comprises the following steps of taking a nanosheet sensor as a working electrode, taking a saturated calomel electrode as a reference electrode, taking a Pt sheet as a counter electrode, soaking the working electrode, the reference electrode and the counter electrode in electrolyte to form a three-electrode system, electrifying, then dropwise adding glucose solutions with different concentrations into the electrolyte, and respectively recording the current sizes corresponding to the glucose solutions with different concentrations; and (3) replacing the electrolyte, dripping a blood sample to be tested into the replaced electrolyte, and recording the current corresponding to the blood sample to obtain the glucose concentration in the blood sample.

10. The ni (oh) for non-enzymatic glucose testing of claim 9₂Use of a nanosheet sensor in the detection of glucose concentration in blood, wherein the electricity isThe hydrolysate is potassium hydroxide solution; the concentration of the potassium hydroxide solution is 0.05M-0.2M.

Background

With the increasing living standard, diabetes becomes a chronic disease worldwide, and related diseases such as stroke, renal failure, heart disease and the like are brought about. Glucose is the main molecule for diabetes monitoring, and the concentration change of glucose has a crucial effect on human health. Accurate measurement of glucose is also of profound significance for future preventive medical diagnostics, food safety, environmental monitoring, pharmaceutical analysis and biotechnology.

Among the many methods for glucose measurement, optical and electrochemical analysis have been widely studied. Optical methods use a color change in the indicator to reflect the concentration of glucose. The color of the dye changes during the enzymatic reaction, which converts glucose into its metabolites. (j.choi, d.kang, s.han, s.b.kim, j.a.rogers, adv.healthcare mater.2017,6,1601355). Although color change provides an intuitive way for a patient to check for the presence of blood glucose, it is still insufficient to quantify glucose levels or to effectively measure low glucose levels. Even if quantitative measurements can be made, it is often necessary to use a cumbersome spectrophotometer, which makes colorimetric methods unsuitable for commercial use. (b. -h.hou, h.takanaga, g.grossmann, l. -q.chen, x. -q.qu, a.m.jones, s.lande, o.schweissgut, w.wiechert, w.b.frommer, nat.protoc.2011,6,1818). Therefore, the spectroscopic glucose detection method is more suitable for use in professional institutions such as hospitals. Electrochemical detection is considered to be an effective method for detecting glucose due to high detection efficiency and high calibration. Currently, electrochemical glucose biosensors are classified into enzymatic biosensors and non-enzymatic biosensors. The non-enzyme biosensor has the characteristics of low price, high efficiency, difficult influence from environmental change, simple preparation process and the like, and has attracted extensive attention of people. The basic components of a high performance non-enzymatic glucose biosensor are electrocatalysts with high sensitivity, long stability and good selectivity.

Defects are often observed in electrocatalysts and can alter the electronic structure, surface absorption characteristics, transport properties, etc. of the material, which can severely affect the reaction kinetics at the catalyst surface. Defect engineering has proven to be an effective method of increasing the electrocatalytic activity of transition metal oxides and hydroxides. For example, Zhao et al used a bottom-up strategy to synthesize ZnAl-LDH nanosheets with varying numbers of oxygen vacancies. By increasing the density of oxygen vacancies, the formation of Zn-Vo complexes promotes CO₂Adsorption and electron transfer processes to enhance photocatalytic CO₂(iv) reduction rate (Zhao, y.f.; Chen, g.b.; Bian, t.; Zhou, c.; Waterhouse, g.i.n.; Wu, l.z.; Tung, c.h.; Smith, l.j.; O' Hare, d.; Zhang, t.r.defect-Rich ultrasalt ZnAl-layred Double Hydroxide Nanosheets for effective reagent catalysis of CO2 to CO with water. adv Mater 2015,27(47), 7824-. One-step synthesis method of Zhang et al prepares the NiFe-LDH nano-material with defects. Oxygen vacancies and cation vacancies in the material jointly enhance the adsorption capacity of Water molecules, enhance the bonding strength of OH intermediates formed in the OER process, and endow the NiFe-LDH with excellent OER reaction performance (Zhang, X.; Zhao, Y.F.; Zhao, Y.X.; Shi, R.; Waterhouse, G.I.N.; Zhang, T.R. ASimple Synthetic strand heated delivery-Ring ports monomer NiFe-Layered Double Hydroxide nanoparticles for Efficient electrochemical adsorption of organic matter. addition Energy Mater2019,9 (24)). Considering that the performance of electrochemical non-enzymatic glucose sensors depends to a large extent on the activity of the electrocatalyst, defect engineering is expected to be a promising approach to improve device sensitivity.

However, the current high-performance glucose catalyst is still in short supply, and the existing glucose catalyst synthesis method generally has high energy consumption and complex synthesis method.

Disclosure of Invention

In order to overcome the defects of the prior art, the invention aims to provide Ni (OH) for non-enzymatic glucose detection₂A nano-sheet sensor and a preparation method and application thereof.

The invention aims to solve the problem of shortage of the current high-performance glucose catalyst and provide a green, energy-saving and simple synthetic method of Ni (OH)₂A method of nanoplatelets.

Another object of the present invention is to treat the nanosheets synthesized by the above-described method with plasma. The invention can control the synthesis of Ni (OH) with different oxygen defects by adjusting the factors such as the power and the time of plasma treatment₂Nanosheets.

It is still another object of the present invention to provide the above Ni (OH)₂Application of the nano-sheet. The Ni (OH)₂The nanoplatelets are used to detect changes in glucose concentration in the body.

It is yet another object of the present invention to utilize the above process to observe the effect of plasma processing on the glucose sensing performance of a material.

The purpose of the invention is realized by at least one of the following technical solutions.

The invention provides Ni (OH) for non-enzymatic glucose detection₂The preparation method of the nanosheet sensor comprises the following steps:

taking the foamed nickel as a working electrode, then adopting a three-electrode system, soaking the foamed nickel in a nickel acetate solution, and carrying out electrodeposition treatment to obtain Ni (OH) load₂Nano-sheet of foamed nickel, plasma treated to introduce defects, obtaining said Ni (OH) for non-enzymatic glucose detection₂Nanosheet sensor (labeled as Ni (OH)₂/NF electrode).

Further, the concentration of the nickel acetate solution is 0.05-0.2 mol/L.

Preferably, the formulation of the nickel acetate solution comprises: and dissolving nickel acetate in deionized water, and uniformly mixing to obtain the nickel acetate solution.

Further, the time of the electrodeposition treatment is 100-500 s.

Further, the current density of the electrodeposition treatment is 1 to 30mA/cm²。

Preferably, the current density of the electrodeposition treatment is 5 to 30mA/cm²。

Furthermore, in the three-electrode system, foamed nickel is used as a working electrode, Ag/AgCl is used as a reference electrode, and Pt is used as a counter electrode.

Further, the plasma treatment employs argon plasma.

The power of the plasma treatment is 160-200W.

Preferably, the power of the plasma treatment is 180W.

Further, the time of the plasma treatment is 0-40 min. Ni (OH) for non-enzymatic glucose assay when plasma treatment time is 0min₂The surface of the nanosheet sensor does not introduce defects. The appearance before and after plasma treatment has no obvious change. Moreover, the invention provides Ni (OH) loaded₂The foam nickel of the nanosheet can be used for non-enzymatic glucose detection whether or not being subjected to plasma treatment, and the glucose sensing performance of the nanosheet is obviously improved after the nanosheet is subjected to plasma treatment.

Preferably, the plasma treatment time is 20-40 min.

Preferably, the foamed nickel is subjected to a cleaning process before being used as a working electrode, the cleaning process comprising: the foamed nickel was ultrasonically cleaned in ethanol and water, respectively, and then rinsed with pure water.

Further preferably, the cleaning process comprises: the foamed nickel is respectively placed in ethanol and water for ultrasonic cleaning for 30min, and then is washed by pure water.

Preferably, the supported Ni (OH)₂The foamed nickel of the nanosheets was washed with deionized water to remove residual solution and dried prior to plasma treatment.

The invention provides Ni (OH) for non-enzymatic glucose detection prepared by the preparation method₂A nanosheet sensor.

The invention provides Ni (OH) for non-enzymatic glucose detection₂Ni (OH) on nanosheet sensor₂The nano-sheet is nano-scale, and the diameter of the nano-sheet is less than or equal to 200 nm.

The invention provides a method for non-enzymatic glucose detectionNi(OH)₂Application of the nanosheet sensor in detecting the concentration of glucose in blood. The application is to judge the concentration change by electric signal conversion.

The invention provides Ni (OH) for non-enzymatic glucose detection₂The application of the nanosheet sensor in detecting the concentration of glucose in blood comprises the following steps:

the Ni (OH) for non-enzymatic glucose detection₂The method comprises the following steps of taking a nanosheet sensor as a working electrode, taking a saturated calomel electrode as a reference electrode, taking a Pt sheet as a counter electrode, soaking the working electrode, the reference electrode and the counter electrode in electrolyte to form a three-electrode system, electrifying, then dropwise adding glucose solutions with different concentrations into the electrolyte, and respectively recording the current sizes corresponding to the glucose solutions with different concentrations; and (3) replacing the electrolyte, dripping a blood sample to be tested into the replaced electrolyte, and recording the current corresponding to the blood sample to obtain the glucose concentration in the blood sample.

The invention provides Ni (OH) for non-enzymatic glucose detection₂In the application of the nanosheet sensor in detecting the concentration of glucose in blood, the electrolyte is a potassium hydroxide solution; the concentration of the potassium hydroxide solution is 0.05-0.2 mol/L.

The invention provides Ni (OH) for non-enzymatic glucose detection₂In the application of the nanosheet sensor in detecting the concentration of glucose in blood, the linear sensitivity concentration of the nanosheet sensor is in the range of 0.001mM-0.5 mM.

Compared with the prior art, the invention has the following advantages and beneficial effects:

(1) the preparation method provided by the invention has the advantages of low raw material price, no pollution to the environment, no need of high-energy reaction such as high temperature and high pressure and the like, and low production cost;

(2) the preparation method provided by the invention synthesizes Ni (OH) in one step by using an electrodeposition method₂The nano sheet has a simple preparation method and can be produced in batch;

(3) in the preparation method provided by the invention, the utilized flexible substrate foamed nickel with high specific surface area has more related applicable substrate selectivity, such as carbon cloth, carbon paper and the like;

(4) the preparation method provided by the invention utilizes a plasma processing technology, is simple in modification method, and can process materials in batches;

(5) the electro-deposition synthesis method used in the preparation method provided by the invention can change the morphology, yield and the like of the obtained material by changing the solution concentration, the reaction time, the substrate area and the like;

(6) the preparation method provided by the invention is simple and easy to operate, and can be used for synthesizing other related transition metal hydroxides and compounds thereof;

(7) the invention provides Ni (OH) for non-enzymatic glucose detection₂After the nanosheet sensor is subjected to plasma treatment, the glucose sensing performance of the nanosheet sensor is obviously improved, and the nanosheet sensor can be used as a simple means for improving the performance of related materials;

(8) the invention provides Ni (OH) for non-enzymatic glucose detection₂The nano-sheet sensor can be applied to the preparation of a blood glucose detector, and has good selectivity and stability.

Drawings

FIG. 1 shows Ni (OH) for non-enzymatic glucose assay prepared in example 3₂A Scanning Electron Microscope (SEM) image of the nanosheet sensor;

FIG. 2 shows Ni (OH) for non-enzymatic glucose assay prepared in example 3₂An X-ray energy spectrum (EDS) image of the nanosheet sensor;

FIG. 3 shows Ni (OH) for non-enzymatic glucose assay prepared in example 3₂High Resolution Transmission Electron Microscopy (HRTEM) images of the nanosheet sensor;

FIG. 4 shows Ni (OH) for non-enzymatic glucose assay prepared in example 5 of the present invention₂A Scanning Electron Microscope (SEM) image of the nanosheet sensor;

FIG. 5 shows Ni (OH) for non-enzymatic glucose assay prepared in example 7 of the present invention₂A Scanning Electron Microscope (SEM) image of the nanosheet sensor;

FIG. 6 shows Ni (OH) for non-enzymatic glucose assay prepared in example 8 of the present invention₂A Scanning Electron Microscope (SEM) image of the nanosheet sensor;

FIG. 7 shows examples 3 and 8Ni (OH) for non-enzymatic glucose detection₂The oxygen defect content contrast curve of the nanosheet sensor;

FIG. 8 shows Ni (OH) for non-enzymatic glucose assay of examples 3, 5, 7 and 8₂A change curve of glucose sensing current of the nanosheet sensor along with processing time;

FIG. 9 shows Ni (OH) for non-enzymatic glucose assays of examples 3, 5, 7 and 8₂The sensitivity curve of the nanosheet sensor;

FIG. 10 shows Ni (OH) for non-enzymatic glucose assay of examples 9 and 10₂Nanosheet sensor selectivity profile.

Detailed Description

The following examples are presented to further illustrate the practice of the invention, but the practice and protection of the invention is not limited thereto. It is noted that the processes described below, if not specifically described in detail, are all realizable or understandable by those skilled in the art with reference to the prior art. The reagents or apparatus used are not indicated to the manufacturer, and are considered to be conventional products available by commercial purchase.

Example 1

(1) Cleaning a foamed nickel substrate, and respectively performing ultrasonic treatment for 30min in ethanol and an aqueous solution;

(2) washing the foamed nickel substrate in the step (1) by pure water to obtain a washed foamed nickel substrate for later use;

(3) synthesis of Ni (OH) by electrodeposition Using three-electrode System₂And (3) nanosheets, wherein the washed foamed nickel substrate in the step (2) is used as a working electrode, Ag/AgCl is used as a reference electrode, and Pt is used as a counter electrode. The foamed nickel substrate was immersed in 250mL of a 0.05mol/L nickel acetate solution at 5mA/cm²Deposited for 100s at a current density of (3) to obtain Ni (OH) for non-enzymatic glucose detection₂A nanosheet sensor.

(4) The obtained Ni (OH) for non-enzymatic glucose detection₂The nano-sheet sensor is cleaned by deionized water, dried and evaluated by a three-electrode system to obtain Ni (OH)₂NF electrode (the Ni (OH) for non-enzymatic glucose detection)₂Nanosheet sensor) glucose sensing performance,wherein Ni (OH) is used for non-enzymatic glucose detection₂Nanosheet sensor (carrying Ni (OH)₂A nano-sheet foamed nickel substrate) as a working electrode, a saturated calomel electrode as a reference electrode, a Pt sheet as a counter electrode, a potassium hydroxide solution with the concentration of 0.05M as an electrolyte, and testing the current response corresponding to glucose with different concentrations to obtain the sensor with the glucose sensitivity of 7684 muA.mM^-1·cm^-2。

Ni (OH) for non-enzymatic glucose assay obtained in example 1₂The nanosheet sensor is of a nanosheet structure, the diameter of the nanosheet sensor is less than or equal to 200nm, and a scanning electron microscope image of the nanosheet sensor can be as shown in fig. 1.

Example 2

(1) Referring to the synthesis method of example 1, but with the nickel acetate solution concentration of 0.2mol/L in step (3), the deposition current density was 20mA/cm²Obtaining Ni (OH) for non-enzymatic glucose detection₂A nanosheet sensor;

(2) evaluation of the Ni (OH) for non-enzymatic glucose assay Using a three-electrode System₂The glucose sensing performance of the nanosheet sensor was tested according to example 1, except that the potassium hydroxide electrolyte concentration was 0.2M, and the glucose sensitivity of the sensor was 15476 μ a · mM^-1·cm^-2。

Example 2 Ni (OH) for non-enzymatic glucose assay₂The nanosheet sensor is of a nanosheet structure, the diameter of the nanosheet sensor is less than or equal to 200nm, and a scanning electron microscope image of the nanosheet sensor can be as shown in fig. 1.

Example 3

(1) Referring to the synthesis method of example 1, but with the nickel acetate solution concentration of 0.1mol/L in step (3), the deposition current density was 10mA/cm²Obtaining a Ni (OH) -loaded₂Nanoflake nickel foam, the Ni (OH) for non-enzymatic glucose detection₂A nanosheet sensor;

(2) evaluation of the Ni (OH) for non-enzymatic glucose assay Using a three-electrode System₂The glucose sensing performance of the nanosheet sensor, the test method referred to example 1, except that the potassium hydroxide electrolyte concentration was 0.1M, and glucose was presentThe sensitivity was 11171. mu.A.mM^-1·cm^-2As shown in fig. 9.

Wherein the obtained Ni (OH) for non-enzymatic glucose detection₂The scanning electron microscope image of the nanosheet sensor is shown in FIG. 1, and the obtained material is of a nanosheet structure, and the diameter of the nanosheet structure is less than or equal to 200 nm.

Wherein the obtained Ni (OH) for non-enzymatic glucose detection₂An X-ray energy spectrum (EDS) imaging image of the nanosheet sensor is shown in FIG. 2, and Ni and O elements of the obtained material are uniformly distributed on the substrate.

Wherein the obtained Ni (OH) for non-enzymatic glucose detection₂The image of the nanosheet sensor taken with a High Resolution Transmission Electron Microscope (HRTEM) is shown in fig. 3, and the Ni and O elements of the obtained material are uniformly distributed on the substrate.

Wherein the obtained Ni (OH) for non-enzymatic glucose detection₂The defect content of the nanosheet sensor is shown in fig. 7, and it was found that the oxygen defect content was rare before the nanosheet sensor was subjected to plasma treatment.

Wherein the resulting modified glucose catalyst Ni (OH)₂The sensing performance of (a) is shown in fig. 8.

Example 4

(1) With reference to the synthesis method of example 3, Ni (OH) -loaded was obtained₂A nano-sheet of foamed nickel;

(2) will load Ni (OH)₂The foamed nickel of the nano-sheets is treated for 20min by Ar plasma with power of 160W to obtain a modified sensor (the Ni (OH) for non-enzymatic glucose detection)₂Nanosheet sensor) followed by glucose sensing performance testing. Test method referring to example 3, the glucose sensitivity of the modified sensor was found to be 11246 μ A. multidot.mM^-1·cm^-2。

Example 4 Ni (OH) prepared for non-enzymatic glucose assay₂Scanning electron microscope images of nanosheet sensors before plasma treatment are shown in FIG. 1, which shows that Ni (OH) for non-enzymatic glucose detection prepared in example 4₂The appearance of the nanosheet sensor after plasma treatment can be seen in fig. 4, and the nanosheet sensor is used for non-enzymatic glucoseDetected Ni (OH)₂The nanosheet sensor retains the nanosheet structure after plasma treatment.

Example 5

(1) With reference to the synthesis method of example 3, Ni (OH) -loaded was obtained₂A nano-sheet of foamed nickel;

(2) will be used for non-enzymatic glucose detection Ni (OH)₂The nanosheet sensor is treated by Ar plasma with power of 180W for 20min to obtain a modified sensor (the Ni (OH) for non-enzymatic glucose detection)₂Nanosheet sensor) followed by glucose sensing performance testing. Test methods refer to example 3.

Example 5 Ni (OH) for non-enzymatic glucose assay₂Scanning electron microscope images of nanosheet sensors before plasma treatment are shown in FIG. 1, which shows Ni (OH) for non-enzymatic glucose detection prepared in example 5₂The shape of the nanosheet sensor after plasma treatment is shown in FIG. 4, and Ni (OH) for non-enzymatic glucose detection is found₂The nanosheet sensor retains the nanosheet structure after plasma treatment.

The sensitivity of the resulting modified sensor is shown in FIG. 8, and the glucose sensitivity is 11340. mu.A. multidot.mM^-1·cm^-2

Example 6

(1) With reference to the synthesis method of example 3, Ni (OH) -loaded was obtained₂A nano-sheet of foamed nickel;

(2) will load Ni (OH)₂The foamed nickel of the nanosheets is treated by Ar plasma with the power of 200W for 20min to obtain a modified sensor (the Ni (OH) for non-enzymatic glucose detection)₂Nanosheet sensor) followed by glucose sensing performance testing. Test method referring to example 3, the glucose sensitivity of the resulting modified sensor was 11450. mu.A.mM^-1·cm^-2。

Example 6 Ni (OH) for non-enzymatic glucose assay₂Scanning electron microscope images of the nanosheet sensor before plasma treatment can be seen in fig. 1, and the nanosheet sensor is used for non-enzymatic glucose detection and is prepared in example 6Ni (OH)₂The shape of the nanosheet sensor after plasma treatment can be seen in FIG. 4, and the Ni (OH) for non-enzymatic glucose detection is found₂The nanosheet sensor retains the nanosheet structure after plasma treatment.

Example 7

(1) With reference to the synthesis method of example 3, Ni (OH) -loaded was obtained₂A nano-sheet of foamed nickel;

(2) will load Ni (OH)₂The foamed nickel of the nanosheets is treated by Ar plasma with the power of 180W for 30min to obtain a modified sensor (the Ni (OH) for non-enzymatic glucose detection)₂Nanosheet sensor) followed by glucose sensing performance testing. Test methods refer to example 3.

Example 7 Ni (OH) for non-enzymatic glucose assay₂Scanning electron microscope images of nanosheet sensors before plasma treatment are shown in FIG. 1, which shows Ni (OH) for non-enzymatic glucose detection prepared in example 7₂The shape of the nanosheet sensor after plasma treatment is shown in FIG. 5, and Ni (OH) for non-enzymatic glucose detection is found₂The nanosheet sensor retains the nanosheet structure after plasma treatment.

Wherein the sensing performance of the resulting modified sensor is shown in fig. 8.

The sensitivity of the resulting modified sensor is shown in FIG. 9, and the resulting glucose sensitivity is 11993. mu.A. multidot.mM^-1·cm^-2

Example 8

(1) With reference to the synthesis method of example 3, Ni (OH) -loaded was obtained₂A nano-sheet of foamed nickel;

(2) will load Ni (OH)₂The foamed nickel of the nanosheets is treated by Ar plasma with the power of 180W for 40min to obtain a modified sensor (the Ni (OH) for non-enzymatic glucose detection)₂Nanosheet sensor) followed by glucose sensing performance testing. Test methods refer to example 3.

Example 8 Ni (OH) prepared for non-enzymatic glucose assay₂Nanosheet sensingScanning electron microscope image of the device before plasma treatment can be seen from FIG. 1, Ni (OH) for non-enzymatic glucose assay prepared in example 8₂The appearance of the nanosheet sensor after plasma treatment can be seen in FIG. 6, which shows that the nanosheet sensor is Ni (OH) for non-enzymatic glucose detection₂The nanosheet sensor retains the nanosheet structure after plasma treatment.

Examples 3 and 8 Ni (OH) for non-enzymatic glucose assay₂The oxygen defect content of the nanosheet sensor is compared with the curve, and as can be seen from fig. 7: example 8 Ni (OH) prepared for non-enzymatic glucose assay₂The nanosheet sensor has more oxygen defects after plasma treatment.

The sensing performance of the modified sensor obtained in this example is shown in fig. 8. Prsistine in FIG. 8 represents Ni (OH) for non-enzymatic glucose assay prepared in example 3₂A nanosheet sensor, Ar-20min represents the modified sensor prepared in example 5, Ar-30min represents the modified sensor prepared in example 7, and Ar-40min represents the modified sensor prepared in example 8. From fig. 8, it can be found that: compared with a sensor without plasma treatment, the sensor after plasma treatment has better sensing performance, and the glucose sensing performance of the sensor after plasma treatment is obviously improved.

The sensitivity of the sensor obtained in this example after modification is shown in FIG. 9, and the obtained glucose sensitivity is 13940. mu.A. multidot.mM^-1·cm^-2. Prsistine in FIG. 9 represents Ni (OH) for non-enzymatic glucose assay prepared in example 3₂A nanosheet sensor, Ar-20min represents the modified sensor prepared in example 5, Ar-30min represents the modified sensor prepared in example 7, and Ar-40min represents the modified sensor prepared in example 8.

Example 9

(1) With reference to the synthesis method of example 3, Ni (OH) -loaded was obtained₂Nanoflake nickel foam (Ni (OH) for non-enzymatic glucose detection)₂Nanosheet sensors);

(2) will load Ni (OH)₂The preparation method comprises the steps of taking foamed nickel of a nanosheet as a working electrode, taking a saturated calomel electrode as a reference electrode, taking a Pt sheet as a counter electrode, soaking the working electrode, the reference electrode and the counter electrode in electrolyte (potassium hydroxide solution is selected) to form a three-electrode system, electrifying, respectively adding 1mmol/L glucose, 0.1mol/L sodium chloride, 0.1mol/L uric acid, 0.1mol/L ascorbic acid and 0.1mol/L sucrose into 0.1mol/L electrolyte at an interval of 60s, and finally adding 1mmol/L glucose. Thereby, the selectivity test of the material is carried out.

Wherein the resulting Ni (OH) is used for non-enzymatic glucose detection₂The selectivity test of the nanosheet sensor is shown in fig. 10. In FIG. 10, Ni (OH)₂Represent Ni (OH) for non-enzymatic glucose assay prepared in example 9₂A nanosheet sensor, Ar-40min in FIG. 10, represents the modified sensor prepared in example 10.

Example 10

(1) With reference to the synthesis procedure of example 8, a modified sensor (Ni (OH) for non-enzymatic glucose detection) was obtained₂Nanosheet sensors);

(2) the modified sensor is used as a working electrode, a saturated calomel electrode is used as a reference electrode, a Pt sheet is used as a counter electrode, the working electrode, the reference electrode and the counter electrode are all soaked in electrolyte (potassium hydroxide solution is selected) to form a three-electrode system, the three-electrode system is electrified, under the condition that 0.6V bias is applied, 1mmol/L glucose, 0.1mol/L sodium chloride, 0.1mol/L uric acid, 0.1mol/L ascorbic acid and 0.1mol/L cane sugar are respectively added into 0.1mol/L electrolyte at an interval of 60s, and finally 1mmol/L glucose is added. Thereby, the selectivity test of the material is carried out.

Wherein the selectivity test of the resulting modified sensor is shown in figure 10. In FIG. 10, Ni (OH)₂Represent Ni (OH) for non-enzymatic glucose assay prepared in example 9₂A nanosheet sensor, Ar-40min in FIG. 10, represents the modified sensor prepared in example 10.

As can be seen from FIG. 10, Ni prepared in example 9 for non-enzymatic glucose assay(OH)₂The nanosheet sensor and the modified sensor prepared in example 10 also had good selectivity for glucose, but the modified sensor prepared in example 10 (the sensor after plasma treatment) had better selectivity for glucose.

The above examples are only preferred embodiments of the present invention, which are intended to be illustrative and not limiting, and those skilled in the art should understand that they can make various changes, substitutions and alterations without departing from the spirit and scope of the invention.