1. The utility model provides an automatic fluorescence detection equipment, includes lower organism (1), upper computer body (5) and PC (6), its characterized in that: the bottom of the lower machine body (1) is provided with a base (2), the upper end of the lower machine body (1) is provided with an upper machine body (5), the upper machine body (5) is provided with a PC (personal computer) machine (6), the rear part of the PC machine (6) is provided with a rear cover shell (12), the left end of the upper end face of the base (2) is vertically fixed with a left fixing plate (15), the right end of the upper end face of the base (2) is vertically fixed with a right fixing plate (25), the middle parts of the left fixing plate (15) and the right fixing plate (25) are connected with a cross frame plate (17), and the upper ends of the left fixing plate (15) and the right fixing plate (25) are supported on the bottom surface of a top plate (28);

the microscope (21) is installed in the middle of the top plate (28), the movable plate (29) is arranged at the front end of the lower portion of the microscope (21), the rear fixing plate (22) is arranged at the rear end of the lower portion of the microscope (21), the objective lens (26) is installed on the lower portion of the movable plate (29), the lower end of the objective lens (26) faces the moving platform (27), and the moving platform (27) is located in the middle of the upper end face of the base (2).

2. An automated fluorescence detection device according to claim 1, wherein: the upper end surface of the moving platform (27) is provided with a mounting groove (31), the mounting groove (31) is provided with a glass carrying table (30), the mounting groove (31) moves horizontally on the moving platform (27) in a front-back direction, and the mounting groove (31) moves horizontally on the moving platform (27) in a left-right direction.

3. An automated fluorescence detection device according to claim 1, wherein: an open slot (3) is formed in the middle of the front side face of the lower machine body (1), a scanning port (4) is formed in the open slot (3), and the front end of the mounting slot (31) extends out of the scanning port (4).

4. An automated fluorescence detection device according to claim 1, wherein: the left side and the right side of the lower machine body (1) are both provided with outer side plates (10), the upper portions of the outer side plates (10) are provided with second heat dissipation frames (11), the lower portions of the outer side plates (10) are provided with first heat dissipation frames (9), and the outer side plates (10) are made of alloy materials.

5. An automated fluorescence detection device according to claim 1, wherein: the novel microscope is characterized in that a third heat dissipation frame (13) is arranged on the left side face and the right side face of a rear housing (12) at the rear part of the PC (6), a display screen (8) is arranged on the upper portion of the front side face of the PC (6), a control button (7) is arranged on the lower portion of the front side face of the PC (6), and the microscope (21) is located in the middle of the rear housing (12).

6. An automated fluorescence detection device according to claim 1, wherein: base (2), left fixed plate (15), cross frame board (17), right fixed plate (25) and roof (28) constitute automatic fluorescence check out test set's internal frame structure, and the rear portion of left fixed plate (15) is fixed with connecting plate (16) to the left surface upper portion of connecting plate (16) is fixed with left fixed block (19), and the rear portion of right fixed plate (25) is fixed with baffle (24), and installs battery module (23) on baffle (24) and adopt lithium battery.

7. An automated fluorescence detection device according to claim 1, wherein: the rear side surface of the rear fixing plate (22) is provided with an automatic focusing structure (20), the right lower part of the rear side surface of the lower machine body (1) is provided with a user interface (14), and the user interface (14) and the automatic focusing structure (20) are electrically connected with a controller in the PC (6).

8. An automated fluorescence detection device according to claim 1, wherein: the left part of the upper end face of the transverse frame plate (17) is provided with a light source switching mechanism (18), a light source module (32) is arranged on the right side of the light source switching mechanism (18), and the right port of the light source module (32) faces the lower part of the microscope (21).

9. An automated fluorescence detection device according to claim 1, wherein: the movable plate (29) moves vertically and straightly at the front end of the lower part of the microscope (21), the objective lens (26) arranged at the lower end of the movable plate (29) and the movable plate (29) keep synchronous moving, the objective lens (26) and the movable plate (29) are connected in a split mode, and the centers of the microscope (21), the objective lens (26) and the glass carrying platform (30) are all located on the same axis.

Background

A flow cytometer used in medical and biological fields incorporates a fluorescence detection device that receives fluorescence emitted by a fluorescent dye of a measurement target upon irradiation with laser light to identify the type of the measurement target, and particularly in the medical field, studies on interactions such as biological binding between proteins have been actively conducted, and studies on interactions between proteins have been actively conducted by using a measurement method of fluorescence resonance energy transfer. Conventionally, FRET measurement is performed by changing fluorescence intensity, but in recent years, various flow cytometers have been disclosed which analyze a measurement target by using a change in fluorescence relaxation time.

In general, in a flow cytometer, a turbid solution of a measurement target substance such as a living material containing cells represented by proteins, DNA, RNA, and enzymes is labeled with a fluorescent dye, and then a pressure is applied so that a sheath solution flows through a channel at a speed of 10m or less per second and the turbid solution containing the measurement target substance labeled with the fluorescent dye flows through the sheath solution; the flow cell of the object to be measured is thus formed, and when the object to be measured in the flow is irradiated with laser light, the flow cytometer receives fluorescence emitted from the fluorescent dye attached to the object to be measured, and recognizes the fluorescence as a label, thereby specifying the object to be measured.

Flow cytometers, for example, can measure the relative amounts of measurement objects such as DNA, RNA, enzymes, and proteins in cells in the cells and analyze the effects of these measurement objects in a short time, and use cell classifiers that specify cells or chromosomes of a specific type by fluorescence and sort and collect only specific cells or chromosomes in a live state in a short time.

However, in the prior art, all fluorescence detection devices require a worker to manually operate a microscope for detection, and manually photograph, store data information and export data information, so that the working procedure is complicated, the working efficiency is low, and errors may occur.

Disclosure of Invention

The invention aims to provide automatic fluorescence detection equipment to solve the problems that the fluorescence detection equipment provided by the background technology needs workers to manually operate a microscope for detection, manually photograph, store data information and export data information, the working procedure is complicated, the working efficiency is low, and errors are possible.

In order to achieve the purpose, the invention provides the following technical scheme: an automatic fluorescence detection device comprises a lower machine body, an upper machine body and a PC (personal computer), wherein a base is arranged at the bottom of the lower machine body, the upper machine body is arranged at the upper end of the lower machine body, the PC is arranged on the upper machine body, a rear cover shell is arranged at the rear part of the PC, a left fixing plate is vertically fixed at the left end of the upper end surface of the base, a right fixing plate is vertically fixed at the right end of the upper end surface of the base, a cross frame plate is connected between the middle parts of the left fixing plate and the right fixing plate, and the upper ends of the left fixing plate and the right fixing plate are supported on the bottom surface of a top plate;

the microscope is installed at the middle part of the top plate, a movable plate is arranged at the front end of the lower part of the microscope, a rear fixing plate is arranged at the rear end of the lower part of the microscope, an objective lens is installed at the lower part of the movable plate, the lower end of the objective lens faces the moving platform, and the moving platform is located in the middle of the upper end face of the base.

Further, a mounting groove is installed on the upper end face of the moving platform, a glass carrying table is arranged on the mounting groove, the mounting groove moves horizontally on the moving platform in a front-back mode, and the mounting groove moves horizontally on the moving platform in a left-right mode.

Furthermore, an open slot is formed in the middle of the front side face of the lower machine body, a scanning port is formed in the open slot, and the front end of the mounting slot extends out of the scanning port.

Further, the left side and the right side of the lower machine body are both provided with outer side plates, the upper portions of the outer side plates are provided with second heat dissipation frames, the lower portions of the outer side plates are provided with first heat dissipation frames, and the outer side plates are made of alloy materials.

Furthermore, a third heat dissipation frame is arranged on the left side face and the right side face of the rear cover shell at the rear part of the PC, a display screen is arranged on the upper portion of the front side face of the PC, a control button is arranged on the lower portion of the front side face of the PC, and the microscope is located in the middle of the rear cover shell.

Further, base, left fixed plate, cross frame plate, right fixed plate and roof constitute automatic fluorescence check out test set's inner frame structure, and the rear portion of left fixed plate is fixed with the connecting plate to the left surface upper portion of connecting plate is fixed with left fixed block, and the rear portion of right fixed plate is fixed with the baffle, and installs battery module on the baffle and adopt lithium battery.

Furthermore, an automatic focusing structure is installed on the rear side face of the rear fixing plate, a user interface is arranged on the right lower portion of the rear side face of the lower machine body, and the user interface and the automatic focusing structure are electrically connected with a controller in the PC.

Further, a light source switching mechanism is mounted on the left portion of the upper end face of the transverse frame plate, a light source module is arranged on the right side of the light source switching mechanism, and a right port of the light source module faces the lower portion of the microscope.

Furthermore, the movable plate moves vertically at the front end of the lower part of the microscope, the objective lens arranged at the lower end of the movable plate and the movable plate keep synchronous movement, the objective lens is connected with the movable plate in a split mode, and the centers of the microscope, the objective lens and the glass carrying platform are all located on the same axis.

Compared with the prior art, the invention has the beneficial effects that:

1. the moving platform is provided with X, Y shaft two moving directions, each moving direction is driven by a high-precision stepping motor and a high-precision lead screw, a mounting groove on the moving platform can be conveniently moved left and right or front and back, a glass carrying table arranged on the mounting groove is used for placing glass slides, the moving platform is high in arrangement and moving precision, and the moving platform is more flexible to use.

2. According to the invention, the scanning port is formed in the open slot formed in the front side surface of the lower machine body, and the front end of the mounting groove on the mobile platform conveniently extends out of the scanning port when moving forwards, so that a detection worker can place a glass slide on the mounting groove, the glass slide can be placed quickly by extending in and out, the glass slide is placed simply, and the use is more intelligent.

3. The base, the left fixing plate, the transverse frame plate, the right fixing plate and the top plate are fixed together to form an internal frame structure, namely an inner housing structure, the microscope is installed in the middle of the upper portion of the inner housing structure, the moving platform is installed in the middle of the lower portion of the inner housing structure, the objective lens installed on the lower portion of the microscope and the center of the moving platform are on the same axis, and therefore the upper portion and the lower portion of the microscope can be conveniently aligned to detect samples on a glass slide.

4. The left part of the upper end face of the transverse frame plate is provided with a light source switching mechanism and a light source module, an automatic focusing light source in the automatic focusing device is a 785nm 80-100mW laser diode, the automatic focusing light source is mainly controlled by an LED control module on a controller in a PC (personal computer), and a constant current driver of a laser tube and the laser tube are integrated in an automatic focusing module together.

5. The movable plate is arranged on the front side of the lower portion of the microscope and can move upwards or downwards in the vertical direction, and the objective lens is mounted on the lower portion of the movable plate, so that the movable plate conveniently drives the objective lens to move synchronously, the height position of the objective lens is conveniently adjusted in the vertical direction, the movable plate is conveniently used for detecting a sample on a lower glass slide, and the movable plate is flexible to use.

Drawings

FIG. 1 is a schematic perspective view of the structure of the present invention;

FIG. 2 is a schematic front view of the structure of the present invention;

FIG. 3 is a schematic left side view of the structure of the present invention;

FIG. 4 is a schematic right-view of the structure of the present invention;

FIG. 5 is a schematic rear view of the structure of the present invention;

FIG. 6 is a schematic top view of the structure of the present invention;

FIG. 7 is a bottom view of the structure of the present invention;

FIG. 8 is a schematic perspective view of the internal structure of the present invention;

FIG. 9 is a schematic front view of the internal structure of the present invention;

FIG. 10 is a schematic view of the upper end face structure of the mobile platform of the present invention;

FIG. 11 is a block diagram of a control system of the present invention;

FIG. 12 is a schematic diagram of a power supply configuration of the present invention;

FIG. 13 is a structural diagram of an auto-focusing function according to the present invention.

In the figure: 1. a lower machine body; 2. a base; 3. an open slot; 4. a scanning port; 5. mounting the machine body; 6. a PC machine; 7. a control button; 8. a display screen; 9. a first heat dissipation frame; 10. an outer panel; 11. a second heat dissipation frame; 12. a rear housing; 13. a third heat dissipation frame; 14. a user interface; 15. a left fixing plate; 16. a connecting plate; 17. a transverse frame plate; 18. a light source switching mechanism; 19. a left fixed block; 20. an auto-focus structure; 21. a microscope; 22. a rear fixing plate; 23. a battery module; 24. a baffle plate; 25. a right fixing plate; 26. an objective lens; 27. a mobile platform; 28. a top plate; 29. a movable plate; 30. a glass loading table; 31. mounting grooves; 32. a light source module.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In a first embodiment, referring to fig. 1 to 13, the present invention provides a technical solution: an automatic fluorescence detection device comprises a lower machine body 1, an upper machine body 5 and a PC 6, wherein the bottom of the lower machine body 1 is provided with a base 2, the upper end of the lower machine body 1 is provided with the upper machine body 5, the upper machine body 5 is provided with the PC 6, the rear part of the PC 6 is provided with a rear cover 12, the left end of the upper end surface of the base 2 is vertically fixed with a left fixing plate 15, the right end of the upper end surface of the base 2 is vertically fixed with a right fixing plate 25, the middle parts of the left fixing plate 15 and the right fixing plate 25 are connected with a cross frame plate 17, the upper ends of the left fixing plate 15 and the right fixing plate 25 are supported on the bottom surface of a top plate 28, the middle part of the top plate 28 is provided with a microscope 21, the front end of the lower part of the microscope 21 is provided with a movable plate 29, the rear end of the lower part of the microscope 21 is provided with a rear fixing plate 22, the lower part of the movable plate 29 is provided with an objective lens 26, and the lower end of the objective lens 26 faces a movable platform 27, and the moving platform 27 is located at the middle of the upper end surface of the base 2.

When the automatic fluorescence detection device is used, firstly, the control button 7 at the lower part of the front side surface of the PC 6 is pressed to start the automatic fluorescence detection work, at this time, the installation groove 31 on the moving platform 27 starts to move horizontally forwards, so that the front part of the installation groove 31 extends out of the scanning port 4, so that a detection worker can place a glass slide in the glass carrying platform 30 on the installation groove 31, after the control button 7 is pressed again, the installation groove 31 automatically moves backwards and returns to the moving platform 27 completely, meanwhile, the installation groove 31 moves horizontally left and right above the moving platform 27 so as to return to the central position, the centers of the newspaper, the microscope 21 and the objective lens 26 are on the same axis, then the glass carrying platform 30 at the lower part of the microscope 21 carries out straight lifting movement according to the hole opening position on the glass slide, drives the objective lens 26 to move together in the pen direction, so as to move the objective lens 26 to the proper height, the light source switching mechanism 18 and the light source module 32 on the left part of the upper end face of the transverse frame plate 17 expand light beams, the microscope 21 carries out automatic focusing and amplifies a detection sample, then photographs the amplified image, and carries out local data storage on the collected sample picture on the PC 6, the image can be displayed through the display screen 8, and the data line can be connected through the user interface 14 to be led out to external equipment.

In the second embodiment, the first embodiment is further limited, the moving platform 27 of the present invention has two motion directions of X, Y axes, each motion direction is driven by a high precision stepping motor and a high precision lead screw, so that it is convenient to move the mounting groove 31 on the moving platform 27 left and right or forward and backward, and the glass slide table 30 provided on the mounting groove 31 is used for placing the glass slide, so that the moving platform 27 has high setting and moving precision and is more flexible to use.

In the present invention, the scanning port 4 is opened in the open slot 3 formed in the front side surface of the lower body 1, and the front end of the mounting groove 31 on the moving platform 27 conveniently extends out of the scanning port 4 when moving forward, so that the detection worker can place the slide glass on the mounting groove 31, the extension and the extension are fast, the slide glass placement is simple, and the use is more intelligent.

Fourth embodiment, this embodiment is a further limitation to the first embodiment, in the present invention, the outer side plates 10 are disposed on both the left side surface and the right side surface of the lower machine body 1, the second heat dissipation frame 11 and the first heat dissipation frame 9 are disposed on the upper portion and the lower portion of the outer side plates 10, respectively, and the circular ventilation holes are disposed on the second heat dissipation frame 11 and the first heat dissipation frame 9, so that heat generated by the internal machine equipment of the lower machine body 1 is evacuated to the first heat dissipation frame 9 and the second heat dissipation frame 11 acting on both sides during the use process, thereby avoiding the internal temperature from being too high.

In the invention, the rear housing 12 is arranged at the rear part of the PC 6, the rear housing 12 is convenient to arrange to protect the microscope 21, and has a dustproof and waterproof shielding effect, and meanwhile, the third heat dissipation frames 13 are arranged on the left side surface and the right side surface of the rear housing 12, so that the microscope 21 can dissipate heat through the third heat dissipation frames 13 on the left side surface and the right side surface, thereby ensuring good operation of the microscope and prolonging the service life.

Sixth embodiment, the present embodiment is further limited to the first embodiment, the base 2, the left fixing plate 15, the cross frame plate 17, the right fixing plate 25 and the top plate 28 in the present invention are fixed together and together form an internal frame structure, that is, an inner housing structure, the microscope 21 is installed in the middle of the upper portion of the inner housing structure, the moving platform 27 is installed in the middle of the lower portion of the inner housing structure, the objective lens 26 installed in the lower portion of the microscope 21 and the center of the moving platform 27 are on the same axis, so as to be conveniently aligned up and down and detect a sample on a slide, the battery module 23 is a lithium battery, which is convenient for charging and storing electricity, and can be directly used by a power supply, thus being various and more practical.

Seventh embodiment, the present embodiment is further limited to the first embodiment, in the present invention, an auto-focusing mechanism 20 is installed at the rear portion of the rear fixing plate 22, and the user interface 14 and the auto-focusing mechanism 20 are electrically connected to a controller in the PC 6, so as to perform automatic operation.

Eighth embodiment, the present embodiment is a further limitation to the first embodiment, in the present invention, the light source switching mechanism 18 and the light source module 32 are installed on the left portion of the upper end face of the cross frame plate 17, and the auto-focusing light source in the present invention is a 785nm, 80-100mW laser diode, which is mainly controlled by an LED control module on a controller in the PC 6, and the constant current driver of the laser tube is integrated with the laser tube in the auto-focusing module.

In the ninth embodiment, which is a further limitation to the first embodiment, a movable plate 29 is disposed at the front side of the lower portion of the microscope 21, the movable plate 29 can move up and down in the vertical direction, and the objective lens 26 is mounted at the lower portion of the movable plate 29, so that the movable plate 29 can conveniently drive the objective lens 26 to move synchronously, and the height position of the objective lens 26 can be adjusted in the vertical direction, so that the objective lens 26 can be conveniently used for detecting a sample on a lower slide, and the use is flexible.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.