1. The composite microbial inoculum is characterized by comprising bacillus mucilaginosus, bacillus laterosporus and azotobacter vinelandii in a weight ratio of 1-2: 1: 1-2.

2. A preparation method of a flower nutrient solution is characterized by comprising the following steps:

(1) adding the compound microbial inoculum of claim 1 into tea water, fermenting for 1-3 days, filtering, and taking filtrate to obtain first fermentation liquor;

(2) adding sheep manure, monopotassium phosphate, ferrous sulfate and compound amino acid into the primary fermentation liquid, performing secondary fermentation, filtering, and taking filtrate to obtain the flower nutrient solution.

3. The method as claimed in claim 2, wherein the tea leaves used in the tea water are oolong tea and/or black tea;

the mass volume ratio of the tea leaves to the brewing water is 1-2 g: 50-60 mL;

the tea water is placed for 10-16 hours.

4. The method according to claim 3, wherein the volume ratio of the mass of the added complex microbial inoculum to the tea water is 1-2 g: 1L of the compound.

5. The method according to claim 4, wherein the temperature of the fermentation in the step (1) is 30-38 ℃, and the rotation speed of the fermentation is 100-120 rpm.

6. The method according to claim 5, wherein the ratio of the mass of the added sheep manure to the volume of the tea water is 3-5 g: 1L;

the sheep manure is dry sheep manure.

7. The method as claimed in claim 6, wherein the volume ratio of the added mass of the monopotassium phosphate to the tea water is 0.5-1.5 g: 1L;

the volume ratio of the added mass of the ferrous sulfate to the tea water is 0.5-1.5 g: 1L;

the volume ratio of the added mass of the compound amino acid to the tea water is 0.5-1.5 g: 1L of the compound.

8. The method according to any one of claims 2 to 7, wherein the temperature of the secondary fermentation in the step (2) is 30 to 38 ℃, the fermentation time is 10 to 15 days, and the fermentation rotating speed is 100 to 120 rpm.

9. A flower nutrient solution prepared by the method of any one of claims 2 to 8.

10. Use of the floral nutrient solution of claim 9 in bougainvillea spectabilis plant cultivation.

Background

Bougainvillea spectabilis Willd (academic name: Bougainvillea spectabilis Willd), alias: jiuhega, trifoliate-leaf plum, Maobaohu, trifoliate-leaf rhododendron, triangular flower, leafflower, leaf plum, paper flower, tissue flower, south American jasmine and the like. Is evergreen climbing shrub. Bougainvillea spectabilis flower is peculiar, bright in color and long in flowering phase, and is often used as an ornamental plant.

When bougainvillea spectabilis is cultivated, the fertilizer is a key factor influencing the growth of the bougainvillea spectabilis, and if the fertilizer is reasonable, the florescence of the bougainvillea spectabilis is prolonged, and the flowers are large; if the fertilizer is not reasonable, the bougainvillea spectabilis flowers are small and few, and even do not bloom. Most of fertilizers for bougainvillea spectabilis in the prior art are cake fertilizers, and no report of cultivating bougainvillea spectabilis by using nutrient solution is found. Therefore, there is a high necessity in the art for a nutrient solution that can be used for cultivating bougainvillea spectabilis to fill the deficiencies of the prior art.

Disclosure of Invention

The invention aims to provide a complex microbial inoculant and a flower nutrient solution prepared from the complex microbial inoculant. The flower nutrient solution prepared by the invention is used for cultivating bougainvillea spectabilis, can effectively prolong the flowering phase of the bougainvillea spectabilis, increases the number of bougainvillea spectabilis flowers and promotes the diameters of the bougainvillea spectabilis flowers to be increased.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides a compound microbial inoculum which comprises bacillus mucilaginosus, bacillus laterosporus and azotobacter vinelandii in a weight ratio of 1-2: 1: 1-2.

The invention also provides a preparation method of the flower nutrient solution, which comprises the following steps:

(1) adding the compound microbial inoculum into tea water, fermenting for 1-3 d, filtering, and taking filtrate to obtain primary fermentation liquor;

(2) adding sheep manure, monopotassium phosphate, ferrous sulfate and compound amino acid into the primary fermentation liquid, performing secondary fermentation, filtering, and taking filtrate to obtain the flower nutrient solution.

Preferably, the tea leaves used in the tea water are oolong tea and/or black tea;

the mass volume ratio of the tea leaves to the brewing water is 1-2 g: 50-60 mL;

the tea water is placed for 10-16 hours.

Preferably, the volume ratio of the added composite microbial inoculum to the tea water is 1-2 g: 1L of the compound.

Preferably, the fermentation temperature in the step (1) is 30-38 ℃, and the fermentation rotating speed is 100-120 rpm.

Preferably, the volume ratio of the added sheep manure to the tea water is 3-5 g: 1L;

the sheep manure is dry sheep manure.

Preferably, the volume ratio of the added mass of the monopotassium phosphate to the tea water is 0.5-1.5 g: 1L;

the volume ratio of the added mass of the ferrous sulfate to the tea water is 0.5-1.5 g: 1L;

the volume ratio of the added mass of the compound amino acid to the tea water is 0.5-1.5 g: 1L of the compound.

Preferably, the temperature of the secondary fermentation in the step (2) is 30-38 ℃, the fermentation time is 10-15 d, and the fermentation rotating speed is 100-120 rpm.

The invention also provides the flower nutrient solution prepared by the method.

The invention also provides application of the flower nutrient solution in bougainvillea spectabilis plant cultivation.

The invention provides a composite microbial inoculum and a flower nutrient solution prepared from the composite microbial inoculum, wherein bacillus mucilaginosus in the composite microbial inoculum can play a role in fixing nitrogen and decomposing phosphorus and potassium; the bacillus laterosporus can inhibit the growth of other pathogenic bacteria; the azotobacter vinelandii has the effects of fixing nitrogen and dissolving phosphorus, and assists the bacillus mucilaginosus to fix nitrogen more effectively and provide nutrition for plants. After the flower nutrient solution prepared from the compound microbial inoculum is used for bougainvillea spectabilis, the growth of other pathogenic bacteria can be inhibited, the flowering phase of the bougainvillea spectabilis can be effectively prolonged, the number of bougainvillea spectabilis flowers is increased, and the diameters of the bougainvillea spectabilis flowers are increased.

Detailed Description

The invention provides a composite microbial inoculum which comprises bacillus mucilaginosus, bacillus laterosporus and azotobacter vinelandii in a weight ratio of 1-2: 1: 1-2, wherein the weight ratio of the composite microbial inoculum is preferably 1.5: 1: 1.5.

bacillus mucilaginosus, Bacillus laterosporus and Azotobacter vinelandii used in the examples of the present invention are all commercially available bacteria.

The invention also provides a preparation method of the flower nutrient solution, which comprises the following steps:

(1) adding the compound microbial inoculum into tea water, fermenting for 1-3 d, filtering, and taking filtrate to obtain primary fermentation liquor;

(2) adding sheep manure, monopotassium phosphate, ferrous sulfate and compound amino acid into the primary fermentation liquid, performing secondary fermentation, filtering, and taking filtrate to obtain the flower nutrient solution.

In the invention, the tea leaves used in the tea water are oolong tea and/or black tea;

the mass volume ratio of the tea leaves to the brewing water is 1-2 g: 50-60 mL, preferably 1.5 g: 55 mL;

the tea water is placed for 10-16 hours, and is preferably placed for 23 hours.

In the invention, the volume ratio of the added composite microbial inoculum to tea water is 1-2 g: 1L, preferably 1.5 g: 1L of the compound.

In the invention, the fermentation temperature in the step (1) is 30-38 ℃, preferably 32-36 ℃, and more preferably 34 ℃;

the rotating speed of the fermentation is 100-120 rpm, preferably 110 rpm.

In the invention, the volume ratio of the added sheep manure to the tea water is 3-5 g: 1L, preferably 4 g: 1L;

the sheep manure is dry sheep manure.

In the invention, the volume ratio of the added mass of the monopotassium phosphate to the tea water is 0.5-1.5 g: 1L, preferably 1 g: 1L;

the volume ratio of the added mass of the ferrous sulfate to the tea water is 0.5-1.5 g: 1L, preferably 1 g: 1L;

the volume ratio of the added mass of the compound amino acid to the tea water is 0.5-1.5 g: 1L, preferably 1 g: 1L of the compound.

In the invention, the temperature of the secondary fermentation in the step (2) is 30-38 ℃, preferably 32-36 ℃, and more preferably 34 ℃;

the fermentation time is 10-15 d, preferably 12-13 d, and further preferably 12.5 d;

the rotating speed of the fermentation is 100-120 rpm, preferably 110 rpm.

The dry sheep manure, the monopotassium phosphate, the ferrous sulfate and the compound amino acid are all commercially available reagents.

The invention also provides the flower nutrient solution prepared by the method.

The invention also provides application of the flower nutrient solution in bougainvillea spectabilis plant cultivation.

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.

Example 1

The composite microbial inoculum of the embodiment 1 is prepared by bacillus mucilaginosus, bacillus laterosporus and azotobacter vinelandii according to the weight ratio of 2:1: 1.

Example 2

The composite microbial inoculum of the embodiment 2 is prepared by bacillus mucilaginosus, bacillus laterosporus and azotobacter vinelandii according to the weight ratio of 1:1: 2.

Example 3

Adding 20g of Tieguanyin into 1L of boiling water, standing for 12h, filtering, and collecting filtrate to obtain tea water. 2g of the compound microbial inoculum of the example 1 is added into tea water, fermented for 2d at 37 ℃ and 100rpm, filtered, and the filtrate is taken to obtain the first fermentation broth.

Adding 3g of dry sheep manure, 1.5g of potassium dihydrogen phosphate, 0.5g of ferrous sulfate and 1g of compound amino acid into the primary fermentation liquid, fermenting for 10 days at 37 ℃ and 100rpm, and filtering to obtain filtrate to obtain the flower nutrient solution of the embodiment 3.

Example 4

Adding 10g Junmei into 1L boiled water, standing for 16 hr, filtering, and collecting filtrate to obtain tea water. Adding 1g of the compound microbial inoculum of the example 2 into tea water, fermenting for 3d at 30 ℃ and 120rpm, filtering, and taking filtrate to obtain first fermentation liquor.

Adding 5g of dry sheep manure, 0.5g of monopotassium phosphate, 1g of ferrous sulfate and 1.5g of compound amino acid into the primary fermentation liquid, fermenting for 15 days at 30 ℃ and 120rpm, and filtering to obtain filtrate to obtain the flower nutrient solution of the embodiment 4.

Application example 1

Selecting 9 pots in 10 days 2 months in 2020, culturing under the same condition, wherein the cutting age is 2 years, the growth condition is the same, and the cherry bougainvillea glabra does not bloom. It was randomly divided into 3 groups of 3 replicates each. The first group was sprayed with the flower nutrient solution of example 3, the second group was sprayed with the flower nutrient solution of example 4, and the third group was sprayed with tap water. The three groups have the same culture mode except that the types of the nutrient solution sprayed on the three groups are different. The experimental stage is started after 2 months and 10 days, different nutrient solutions are sprayed on the day, the spraying parts are branches and leaves of flowers, and the bougainvillea spectabilis leaves are preferably dripped downwards, and the liquid is sprayed for 1 time per month. Recording the growth time of each group of bougainvillea trigonata buds, the number of the initial buds, the diameter of each flower after flowering and the flowering period. The results of the observation are shown in Table 1.

TABLE 1 Effect of nutrient solutions on flowering of bougainvillea spectabilis

According to the embodiment, the composite microbial inoculum and the flower nutrient solution prepared by using the composite microbial inoculum are provided, the growth time of the nutrient solution group prepared by the invention is 1 month earlier than that of the bougainvillea trigonocephala in a tap water spraying group, the initial bud number is increased by 1.8-2.5 times, the average flower diameter is increased by 1-2 cm, and the flowering phase is prolonged by 6-27 days.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.