Activated sludge domesticated by taking glucose as co-matrix and application of activated sludge in advanced treatment of coking wastewater
1. A method for domesticating activated sludge capable of deeply treating coking wastewater by taking glucose as a co-matrix is characterized by comprising the following steps:
step 1), taking activated sludge, aerating the activated sludge, recovering the activity of the activated sludge, and then domesticating the activated sludge;
step 2), adopting an SBR process, adding a growth substrate of microorganisms into the reactor, adding coking wastewater according to the proportion of 10 percent of the effective volume of the reactor, supplementing the rest space with deionized water, controlling the aeration intensity, water inlet and water discharge for 12d of each gradient residence time;
step 3), taking an operation cycle every 72 hours, controlling the DO of the SBR to be 3.0 +/-0.5 mg/L, controlling the water temperature T to be 20.1-21.5 ℃, controlling the pH value to be 8.45-8.86, controlling the sludge age to be 25-30 d, and controlling the water discharge ratio in each cycle to be 60%;
and 4) after the suspended solid concentration (MLSS) of the mixed solution reaches 3500 +/-150 mg/L, the sludge settlement ratio of the mixed solution is 15-20%, and the adding amount of the coking wastewater is gradually increased according to the proportion gradient with the effective volume of 10% for next gradient domestication.
2. The method for acclimatizing the activated sludge capable of deeply treating the coking wastewater by using the glucose as the co-substrate according to claim 1, wherein the activated sludge in the step 1) is the residual sludge of a secondary sedimentation tank of an urban sewage treatment plant, the inoculated sludge amount is 4500mg/L, and the coking wastewater in the step 2) is the effluent of the secondary sedimentation tank of the coking sewage treatment plant after being treated by anoxic-aerobic organisms.
3. The method for acclimatizing activated sludge capable of deeply treating coking wastewater by using glucose as co-substrate according to claim 1, wherein the growth substrate of the microorganism in the step 2) is glucose, NH4Cl and NaH2PO4·2H2O。
4. The method for acclimatizing activated sludge capable of deeply treating coking wastewater by using glucose as co-matrix according to claim 3, wherein the effective volume of the reactor in the step 2) is 3L, glucose is 300mg/L as a concentration standard, and glucose and NH are added according to the proportion of C: N: P: 100:5:14Cl and NaH2PO4·2H2O。
5. The method for acclimatizing activated sludge for advanced treatment of coking wastewater by using glucose as co-substrate according to claim 1, wherein the aeration intensity, water feeding and water discharging of SBR are controlled by using a rotameter, a peristaltic pump and a siphon device in the step 2) respectively.
6. The method for acclimatizing activated sludge capable of deeply treating coking wastewater by using glucose as co-substrate according to claim 1, wherein the water inlet time of SBR in the step 3) is 10min, aerobic reaction is carried out for 65h, and after 6.5h of precipitation, water is decanted for 20 min.
7. The method for acclimatizing activated sludge capable of deeply treating coking wastewater by using glucose as co-substrate according to claim 1, wherein the acclimatization is terminated after the acclimatization concentration is 100% in the step 4).
8. The use of the activated sludge acclimatized with glucose as co-substrate according to claim 1 for the deep treatment of coking wastewater.
9. The application of claim 8, wherein the domesticated activated sludge is used in the SBR reactor to perform advanced treatment on the secondary biochemical effluent of the coking wastewater, the wastewater treatment capacity is 2.5L, the inoculation sludge capacity MLSS is 6000mg/L, and DO is 3.0 +/-0.5 mg/L.
10. The use of claim 8, wherein the activated sludge is effective to reduce COD and NH in the coking wastewater4 +The concentration of-N, and also the degradation of phenol, quinoline and pyridine in the wastewater.
Background
Coking wastewater is typical industrial wastewater generated in the processes of coal coking, coal gas purification, coke recovery and coal liquefaction, and has complex and variable components and high toxicity. The effluent after the secondary biochemical treatment still contains various refractory organic matters, such as phenol, cyanogen, aliphatic compounds, heterocyclic compounds and the like, has poor biodegradability and cannot be directly discharged up to the standard. Therefore, how to remove the refractory organic pollutants in the coking wastewater with high efficiency and low cost has become an urgent problem to be solved in the field of sewage treatment.
Biological treatment is widely used due to the characteristics of simple operation, low running cost and the like. The microbial co-metabolism is an important way for metabolizing and mineralizing organic matters, particularly refractory organic matters by a biological method, so that the microbial co-metabolism can be enhanced by additionally adding a co-metabolism matrix, and the refractory organic matters in the coking wastewater are further degraded. At present, the engineering application research of deep treatment of coking wastewater by utilizing microbial co-metabolism is still deficient.
The domestication of the efficient degrading bacteria adapting to the coking wastewater is the key for the biological treatment system to play a role, and the removal effect of the organic matters in the coking wastewater by the activated sludge system is directly determined, so that the research of the domestication method of the activated sludge, which can be effectively used for the advanced treatment of the coking wastewater, in a short time and at a low cost is of great significance.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide an activated sludge domesticated by taking glucose as a co-matrix and an application thereof in advanced treatment of coking wastewater, wherein the domestication time is short, the cost is low, and the advanced treatment of the coking wastewater can be realized.
In order to achieve the purpose, the invention adopts the technical scheme that:
a method for domesticating activated sludge capable of deeply treating coking wastewater by taking glucose as a co-matrix comprises the following steps:
step 1), taking activated sludge, aerating the activated sludge, recovering the activity of the activated sludge, and then domesticating the activated sludge;
step 2), adopting an SBR process, adding a growth substrate of microorganisms into the reactor, adding coking wastewater according to the proportion of 10 percent of the effective volume of the reactor, supplementing the rest space with deionized water, controlling the aeration intensity, water inlet and water discharge for 12d of each gradient residence time;
step 3), taking an operation cycle every 72 hours, controlling the DO of the SBR to be 3.0 +/-0.5 mg/L, controlling the water temperature T to be 20.1-21.5 ℃, controlling the pH value to be 8.45-8.86, controlling the sludge age to be 25-30 d, and controlling the water discharge ratio in each cycle to be 60%;
and 4) after the suspended solid concentration (MLSS) of the mixed solution reaches 3500 +/-150 mg/L, the sludge settlement ratio of the mixed solution is 15-20%, and the adding amount of the coking wastewater is gradually increased according to the proportion gradient with the effective volume of 10% for next gradient domestication.
The activated sludge obtained by domesticating the glucose serving as the co-substrate can be used for the advanced treatment of the coking wastewater. Advanced treatment is carried out on secondary biochemical effluent of coking wastewater by utilizing domesticated activated sludge in an SBR reactor, the wastewater treatment capacity is 2.5L, the inoculated sludge quantity MLSS is 6000mg/L, and DO is 3.0 +/-0.5 mg/L, and the activated sludge can effectively reduce COD and NH in the coking wastewater4 +The concentration of-N, and also the degradation of phenol, quinoline and pyridine in the wastewater.
Compared with the prior art, the invention has the beneficial effects that:
can domesticate the activated sludge which is suitable for the water quality characteristics of low B/C ratio, high concentration ratio of refractory organics and the like of the secondary biochemical effluent of the coking wastewater.
The domestication process has low cost; according to the method, the co-metabolism of the microorganisms can be enhanced only by adding a proper amount of nutrient elements such as glucose and the like, so that the adaptability and the degradation efficiency of the activated sludge microorganisms to refractory organic matters are effectively improved, the use amount of subsequent advanced treatment agents is reduced, and the operation cost is saved.
The method can enrich the tolerant bacteria of the refractory organic matters and promote the degradation of most of the organic matters such as pyridine, quinoline, phenol and the like in the biological effluent.
Drawings
FIG. 1 is a schematic diagram showing the functional group changes of inlet water and outlet water in the acclimatization process of the present invention.
Detailed Description
The embodiments of the present invention will be described in detail below with reference to the drawings and examples.
The invention relates to a method for domesticating activated sludge capable of deeply treating coking wastewater by taking glucose as a co-substrate, which mainly comprises the following steps:
step 1), taking activated sludge, aerating the activated sludge, recovering the activity of the activated sludge, and then performing acclimation. The activated sludge adopted by the invention is the excess sludge of the secondary sedimentation tank of the municipal sewage treatment plant, and the inoculation sludge amount is 4500 mg/L.
And 2) taking the effluent of a secondary sedimentation tank of a coking wastewater treatment plant after anoxic-aerobic biological treatment as the coking wastewater. Adopting SBR process, the effective volume of the reactor is 3L, glucose is 300mg/L as concentration standard, and glucose and NH are added into the reactor according to the proportion of C, N and P being 100, 5 and 14Cl and NaH2PO4·2H2And O is a growth substrate of microorganisms, coking wastewater is added according to the proportion of 10 percent of the effective volume of the reactor, the rest space is supplemented by deionized water, the retention time of each gradient is 12d, and the aeration intensity, water inlet and water discharge of the SBR are respectively controlled by using a rotor flow meter, a peristaltic pump and a siphon device.
Step 3), the water inlet time of SBR is 10min, aerobic reaction is carried out for 65h, after 6.5h of sedimentation, water decanting is carried out for 20min, total 72h is taken as an operation period, the DO of the SBR is controlled to be 3.0 +/-0.5 mg/L, the water temperature T is 20.1-21.5 ℃, the pH value is 8.45-8.86, the sludge age is 25-30 d, and the water drainage ratio in each period is 60%;
and 4), after the suspended solid concentration (MLSS) of the mixed solution reaches 3500 +/-150 mg/L, the sludge settlement ratio of the mixed solution is 15% -20%, the adding amount of the coking wastewater is gradually increased according to the proportion gradient with the effective volume of 10%, the next gradient domestication is carried out, and the domestication can be finished after the domestication concentration is 100%.
The following are specific examples of the present invention.
Example 1
In this embodiment, the effective volume of SBR is 3L, COD of the inlet water of the reactor is about 210mg/L, ammonia nitrogen of the inlet water is 4.3mg/L, the temperature is 20.1-21.5 ℃, MLSS is 3500 + -150 mg/L, pH is 8.45-8.86, and DO is 3.0 + -0.5 mg/L.
In this example, the acclimation process of the activated sludge of the SBR system is as follows:
1) the sludge inoculation amount is 4500mg/L, coking wastewater with the concentration of 10% is added into SBR, and the rest volume is replaced by deionized water, so that the effective volume of the reactor reaches 3L.
2) And starting a rotor flow meter to aerate the SBR, and maintaining the dissolved oxygen of the mixed liquid in the pool at 2.5-3.5 mg/L, the temperature at 21.3-22.6 ℃ and the pH at 8.45-8.86.
3) Starting a coking wastewater inlet pump, wherein the initial coking wastewater inlet flow is 300mL, and the residual volume is supplemented by deionized water.
4) Adding glucose and NH according to the principle that C, N, P is 100, 5 and 14Cl and NaH2PO4·2H2O as a nutrient
5) The operation period of SBR is 72 hours, namely water feeding is 10 minutes, continuous aeration is 65 hours, static sedimentation is 6.5 hours, and water discharging is 20 minutes.
6) And (3) draining by using a siphon device, wherein the drainage ratio per period is 60%, and the sludge age is 25-30 days.
7) The retention time of each gradient is 12d, MLSS in the SBR system reaches 3500 +/-150 mg/L, and the sludge settlement ratio (SV) is 15-20%.
8) According to 4 operation periods, the water inlet concentration is improved by 10 percent, the adding amount of the coking wastewater is gradually increased until the acclimation concentration is 100 percent (namely the raw water concentration), the operation parameters of the reactor are stable after 40 operation periods are accumulated, namely the acclimation of the activated sludge of the SBR system is finished.
9) In the application case, the concentrations of COD and ammonia nitrogen in the domesticated system are both reduced. Specific information is shown in table 1.
10) In the implementation and application case, the organic matters such as phenol, quinoline and pyridine in the domesticated system are reduced and converted into small molecular compounds. Specific information is shown in table 2 and fig. 1.
11) In the application case, after domestication, the relative abundance of the organic matter degrading bacteria Thaurea, Pesudomonas and Blastocatella is increased from 1.25%, 0.06% and 0.02% during inoculation to 8.91%, 3.35% and 10.76% respectively to obtain enrichment.
The activated sludge successfully domesticated in the embodiment 1 is applied to treat the secondary biochemical effluent of the coking wastewater, and the specific application steps and effects are as follows:
example 2
In the embodiment, the effective volume of SBR is 3L, the COD of the inlet water of the reactor is 254mg/L, the ammonia nitrogen of the inlet water is 4.5mg/L, the temperature is 20.9-22.5 ℃, the MLSS is 6500 +/-150 mg/L, the pH is 8.64-8.79, and the DO is 3.0 +/-0.5 mg/L.
1) The sludge inoculation amount MLSS is 6000mg/L, and the effluent of a secondary sedimentation tank of a coking wastewater treatment plant after anoxic-aerobic biological treatment is added into SBR, so that the effective volume of the reactor reaches 2.5L.
2) And starting a rotor flow meter to aerate the SBR, and maintaining the dissolved oxygen of the mixed liquid in the pool at 2.5-3.5 mg/L, the temperature at 20.9-22.5 ℃ and the pH at 8.64-8.79.
3) The coking wastewater inlet pump is started, and the flow rate of 300mL/min is changed to 2.5L of the effective volume of the reactor.
4) Taking the glucose concentration of 200mg/L and 400mg/L as a standard, respectively recording the glucose concentration as a low carbon source group and a high carbon source group, and respectively adding NH into the reactor according to the principle that C, N and P are 100, 5 and 14Cl and NaH2PO4·2H2O is used as nutrient.
6) Glucose is added into the reactor as co-substrate, and the sludge quantity MLSS in the reactor can reach 6500 +/-150 mg/L.
7) The operation period of SBR is 72 hours, namely water feeding is 10 minutes, continuous aeration is 65 hours, static sedimentation is 6.5 hours, and water discharging is 20 minutes.
8) And (4) draining by using a siphon device, wherein the drainage ratio is 64% per cycle, and the sludge age is 25-30 days.
9) In this example, after 7 operating cycles, the measured COD and ammonia nitrogen concentrations in the system were both reduced. Specific information is shown in table 3.
TABLE 1 Change in conventional Water quality index during acclimation
TABLE 2 variation of organic species of influent and effluent during acclimatization
TABLE 3 conventional Water quality index Change in acclimated systems
Index of water quality
Raw water
Low carbon source group
Removal Rate (%)
High carbon source group
Removal Rate (%)
COD(mg/L)
254.21
220.80
13.14%
226.39
10.94%
NH4 +-N(mg/L)
4.51
3.42
24.19%
3.54
21.51%
Therefore, the method has the characteristics of high domestication efficiency, simplicity in operation, low cost and the like. The domesticated microorganisms can adapt to the water quality characteristics of low B/C ratio, high concentration ratio of refractory organic matters and the like of secondary biochemical effluent of coking wastewater, can effectively metabolize a plurality of refractory organic matters such as phenol, pyridine, quinoline and the like, and enrich bacteria with strong degradation capability such as Thaurea, Pesudomonas and the like, and the sludge settleability of the mixed solution is good, and the settlement ratio is 15-20%. The invention is beneficial to the engineering application of the advanced treatment of the coking wastewater, is particularly suitable for the culture and domestication of the activated sludge for the advanced treatment of the refractory organic wastewater such as the coking wastewater and the like, and provides an effective means for the treatment of the industrial wastewater.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
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