1. The utility model provides a new coronavirus nucleic acid detect reagent box, includes box body (1), upper cover (2) and reagent pipe (15), its characterized in that: the reagent box is characterized in that the box body (1) is connected with the upper cover (2) in a folding and rotating manner, a first rectangular groove is arranged in the box body (1), a supporting plate (3) is fixedly connected with the inner wall of the first rectangular groove, the supporting plate (3) is movably sleeved on the outer side of the reagent tube (15), a front cover (5) is connected with the upper surface of the upper cover (2) in a folding and rotating manner, side covers (4) are respectively connected with the left side and the right side of the upper cover (2) in a folding and rotating manner, a plurality of partition plates (6) are fixedly connected with the upper surface of the supporting plate (3), a first transverse rod (7) is respectively and fixedly connected with the left inner wall, the right inner wall and the left side of the first rectangular groove, a second transverse rod (8) is slidably sleeved on the outer side of the first transverse rod (7), and a first spring (9) is fixedly connected between one end of the second transverse rod (8) and the corresponding partition plate (6) and the inner wall of the first rectangular groove respectively, first spring (9) slip cap is established in the outside of corresponding first horizontal pole (7), and the equal fixedly connected with arc (10) of one end that is close to each other in corresponding second horizontal pole (8) in a plurality of second horizontal poles (8), the bottom inner wall fixedly connected with drier layer (12) of first rectangular channel, a plurality of circular through-holes (13) have been seted up to the upper surface of drier layer (12), the last fixed surface of drier layer (12) is connected with spacing seat (14), the outside of spacing seat (14) and corresponding reagent pipe (15), a plurality of shape boxes (16) of returning of outside fixedly connected with of upper cover (2), the inner wall of shape box (16) of returning are equipped with rectangular plate (17), fixedly connected with second spring (18) between the rear side inner wall of the rear side of rectangular plate (17) and the corresponding shape box (16) of returning.

2. The kit for detecting nucleic acid of a novel coronavirus according to claim 1, wherein: the inner walls of the arc-shaped plates (10) are fixedly connected with rubber pads (11), and the rubber pads (11) enable the arc-shaped plates (10) to protect the tube body of the reagent tube (15) in the process of extruding the outer side of the reagent tube (15).

3. The kit for detecting nucleic acid of a novel coronavirus according to claim 1, wherein: the front side fixedly connected with U-shaped pole (19) of upper cover (2), U-shaped groove (20) have been seted up to the upper surface of box body (1), the outside of U-shaped pole (19) and the inner wall movable contact in U-shaped groove (20).

4. The kit for detecting nucleic acid of a novel coronavirus according to claim 1, wherein: the lower surface of the supporting plate (3) is fixedly connected with a sponge cushion (21).

5. The kit for detecting nucleic acid of a novel coronavirus according to claim 1, wherein: the second rectangular channel has all been seted up to the front side of protecgulum (5) and the front side of box body (1), and two second rectangular channel inner walls are first magnet (22) of fixedly connected with and second magnet (23) respectively, first magnet (22) and second magnet (23) magnetic adsorption.

6. The kit for detecting nucleic acid of a novel coronavirus according to claim 1, wherein: the third rectangular channel has all been seted up on the front side of two side covers (4) and the left side and the right side of box body (1), the inner wall fixedly connected with third magnet (24) of the third rectangular channel on side cover (4), the inner wall fixedly connected with fourth magnet (25) of the third rectangular channel on box body (1), third magnet (24) and the fourth magnet (25) magnetic adsorption who corresponds.

7. The kit for detecting nucleic acid of a novel coronavirus according to claim 1, wherein: the outer side of the rectangular plate (17) is connected with the inner wall of the corresponding clip box (16) in a sliding way.

8. The kit for detecting nucleic acid of a novel coronavirus according to claim 1, wherein: a plurality of circular holes are formed in the upper surfaces of the supporting plate (3) and the spongy cushion (21), and the inner walls of the circular holes are in movable contact with the outer sides of the reagent tubes (15).

9. The method for using the kit for detecting the nucleic acid of the novel coronavirus according to any one of claims 1-8, which comprises the following steps:

s1: a plurality of circular holes with the same size as the reagent tubes (15) are formed in the supporting plate (3) and the spongy cushion (21) through the hole digger;

s2: inserting the reagent tube (15) into the corresponding limiting seat (14) through the circular hole, extruding the two corresponding arc-shaped plates (10) in the inserting process of the reagent tube (15), and enabling the two corresponding arc-shaped plates (10) to move towards the direction away from each other;

s3: the corresponding second cross rods (8) are driven to move in the process that the two corresponding arc-shaped plates (10) move towards the direction away from each other, and the second cross rods (8) extrude the corresponding first springs (9) in the moving process;

s4: after the reagent tubes (15) are inserted, the first springs (9) drive the corresponding arc-shaped plates (10) to fix the corresponding reagent tubes (15) through the elasticity of the first springs, so that the reagent tubes (15) cannot shake in the shaking and carrying process, and the purpose of improving the stability is achieved;

s5: after the reagent tube (15) is inserted, the upper cover (2) is rotated downwards, the upper cover (2) drives the shape returning box (16) to move downwards, the front side of the rectangular plate (17) is contacted with the upper end of the reagent tube (15) in the moving process, and the corresponding second spring (18) is extruded;

s6: when the upper cover (2) is completely covered, the first magnet (22) and the third magnet (24) on the front cover (5) and the side cover (4) are magnetically adsorbed with the second magnet (23) and the fourth magnet (25) on the corresponding box body (1) to fix the upper cover (2);

s7: when the upper cover (2) contacts with the upper surface of the box body (1), the U-shaped rod (19) is clamped with the inner wall of the U-shaped groove (20), so that the sealing property in the box body (1) is improved;

s8: in S5, the second spring (18) applies downward pressure to the reagent tube (15) through the elasticity of the second spring, so that a tube cover at the top end of the reagent tube (15) is fastened, and the stability of the reagent tube (15) is further enhanced;

s9: fully absorb the inside moisture of box body (1) through drier layer (12), keep dry in box body (1), circular through-hole (13) make keep ventilating in box body (1), provide a good air for reagent pipe (15).

Background

The existing in vitro amplification detection technology and kit for the new coronavirus nucleic acid are basically based on RT-

A QPCR technology platform for the in vitro amplification of target DNA fragment includes such steps as reverse transcription of RNA to obtain template cDNA, repeated high-temp, low-temp and medium-temp thermal circulation of thermostable DNA polymerase, specific primer probe, dNTPs substrate, template cDNA and magnesium ions in a buffer reaction system, and repeating high-temp, low-temp and medium-temp thermal circulation for denaturing template DNA, renaturation of primer and template, and extension of primer, and fluorescent detection to obtain 2 n-fold amplification of target DNA fragment. Clinical practice shows that the method has more false negative results, and the analysis reason can have the following aspects:

1. conventional quantitative fluorescence PCR has limited sensitivity, requires more cycles to achieve detection due to the smaller number of templates, and often suffers from the generation of primer dimers and other non-specific amplifications, while once a non-specific template is generated, the specific amplification is inhibited and the efficiency of specific amplification is reduced due to the efficiency of specific amplification being comparable to that of substrate and enzyme competition. Especially when crude clinical specimens are amplified, this often leads to the generation of false positive and false negative results in clinical gene diagnosis.

2. Methods for improving the specificity of PCR amplification include a hot start method, a solid paraffin membrane method, an antibody-enzyme method, a double-stranded primer method, and the like. Although the methods can improve the specificity of PCR amplification and the amplification efficiency to a certain extent, the methods are only slightly improved compared with the conventional PCR, have no essential change, and the specific amplification efficiency does not exceed 2n times.

Ordinary nested PCR (nestPCR) is a highly specific PCR which essentially involves designing two pairs of primers, the outer and inner, in a target sequence to be amplified. Firstly, the outer primer is used for amplification, and then the amplification product is taken as a template and amplified by the inner primer, namely two times of continuous PCR reaction are carried out, the method has the advantages of complex operation and long time consumption, the amplification efficiency cannot exceed 2n times in each time of PCR, and meanwhile, the PCR product amplified by the outer primer is taken as the template in the second amplification, so that the pollution of the PCR product is easily caused, and the false positive of the amplification is caused.

When new coronavirus is detected, the reagent box is used for containing reagents, the placing stability of a reagent tube inside the existing reagent box is not high, and when the reagent box is carried and moved, the reagent tube inside the reagent box is easy to shake, so that the reagents are toppled over and the tube body outside the reagent tube is broken, the sealing performance of the reagent tube is poor, and the using requirements cannot be met.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a novel coronaviruse nucleic acid detection kit and a use method thereof, and solves the problems that the reagent tube in the conventional kit is not high in placement stability, and when the kit is carried and moved, the reagent tube in the kit is easy to shake, so that the reagent is toppled, and the tube body outside the reagent tube is broken, so that the sealing performance of the reagent tube is poor.

(II) technical scheme

The invention specifically adopts the following technical scheme for realizing the purpose:

a reagent kit for detecting nucleic acid of new coronavirus comprises a kit body, an upper cover and a reagent tube, wherein the kit body is connected with the upper cover in a folding and rotating manner, a first rectangular groove is arranged in the kit body, a supporting plate is fixedly connected with the inner wall of the first rectangular groove, the supporting plate is movably sleeved on the outer side of the reagent tube, the upper surface of the upper cover is connected with a front cover in a folding and rotating manner, the left side and the right side of the upper cover are respectively connected with a side cover in a folding and rotating manner, a plurality of partition plates are fixedly connected with the upper surface of the supporting plate, the left side inner wall, the right side inner wall and the left side and the right side of each partition plate of the first rectangular groove are respectively and fixedly connected with a first cross rod, a second cross rod is slidably sleeved on the outer side of the first cross rod, a first spring is respectively and fixedly connected between one end of the second cross rod and the corresponding partition plate and the inner wall of the first rectangular groove, a plurality of second cross rods which are close to each other end is fixedly connected with an arc-shaped plate, the bottom inner wall fixedly connected with drier layer of first rectangular channel, a plurality of circular through-holes have been seted up to the upper surface on drier layer, the spacing seat of last fixed surface of drier layer is connected with, the outside of spacing seat and the reagent pipe that corresponds, a plurality of shape boxes of returning of outside fixedly connected with of upper cover, the inner wall of returning the shape box is equipped with the rectangular plate, fixedly connected with second spring between the rear side of rectangular plate and the rear side inner wall of the shape box that returns that corresponds.

Further, the inner walls of the arc-shaped plates are fixedly connected with rubber pads, and the rubber pads enable the arc-shaped plates to protect the tube body of the reagent tube in the process of extruding the outer side of the reagent tube.

Furthermore, the front side of the upper cover is fixedly connected with a U-shaped rod, the upper surface of the box body is provided with a U-shaped groove, and the outer side of the U-shaped rod is in movable contact with the inner wall of the U-shaped groove.

Further, the lower surface of the supporting plate is fixedly connected with a sponge cushion.

Further, a second rectangular groove is formed in the front side of the front cover and the front side of the box body, and the inner walls of the two second rectangular grooves are fixedly connected with a first magnet and a second magnet which are magnetically adsorbed.

Further, third rectangular channels have been all seted up on the front side of two side covers and the left side and the right side of box body, and the inner wall fixedly connected with third magnet of third rectangular channel on the side cover, the inner wall fixedly connected with fourth magnet of the third rectangular channel on the box body, third magnet and the fourth magnet magnetic adsorption who corresponds.

Further, the outer side of the rectangular plate is in sliding connection with the inner wall of the corresponding box.

Further, a plurality of circular holes are formed in the upper surfaces of the supporting plate and the spongy cushion, and the inner walls of the circular holes are in movable contact with the outer sides of the reagent tubes.

The invention also provides a use method of the novel coronavirus nucleic acid detection kit, which comprises the following steps:

s1: a supporting plate and a spongy cushion are provided with a plurality of circular holes with the same size as the reagent tubes through a hole opener;

s2: inserting the reagent tube into the corresponding limiting seat through the circular hole, and extruding the two corresponding arc-shaped plates by the reagent tube in the inserting process so that the two corresponding arc-shaped plates move in the direction away from each other;

s3: the corresponding second cross rod is driven to move in the process that the two corresponding arc-shaped plates move in the direction away from each other, and the second cross rod extrudes the corresponding first spring in the moving process;

s4: after the reagent tube is inserted, the first spring drives the corresponding arc-shaped plate to fix the corresponding reagent tube through the elasticity of the first spring, so that the reagent tube cannot shake in the shaking and carrying process, and the purpose of improving the stability is achieved;

s5: after the reagent tube is inserted, the upper cover is rotated downwards, the upper cover drives the shape returning box to move downwards, the front side of the rectangular plate is contacted with the upper end of the reagent tube in the moving process, and the corresponding second spring is extruded;

s6: when the upper cover is completely covered, the first magnet and the third magnet on the front cover and the side cover are magnetically adsorbed with the second magnet and the fourth magnet on the corresponding box body to fix the upper cover;

s7: when the upper cover contacts with the upper surface of the box body, the U-shaped rod is clamped with the inner wall of the U-shaped groove, so that the sealing property in the box body is improved;

s8: in the S5, the second spring applies downward pressure to the reagent tube through the self elasticity, so that the tube cover at the top end of the reagent tube is fastened, and the stability of the reagent tube is further enhanced;

s9: fully absorb the inside moisture of box body through the drier layer, keep the box body internal drying, circular through-hole makes and keeps ventilating in the box body, provides a good air for the reagent pipe.

(III) advantageous effects

Compared with the prior art, the invention provides a novel coronavirus nucleic acid detection kit and a use method, and the kit has the following beneficial effects:

according to the reagent tube placing device, the arc-shaped plate, the first cross rod, the second cross rod, the first spring and the limiting seat are matched, the first cross rod and the arc-shaped plate are driven by the first spring to elastically fasten the reagent tube, when the box body is shaken and collided, the reagent tube is prevented from shaking left and right and up and down, the rubber pad prevents the tube body outside the reagent tube from being broken due to collision, and the placing stability of the reagent tube is improved.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic cross-sectional view of the present invention;

FIG. 3 is a schematic sectional perspective view of the clip case;

FIG. 4 is an enlarged perspective view of portion A of FIG. 2;

FIG. 5 is an enlarged perspective view of the portion B in FIG. 2;

fig. 6 is an enlarged perspective view of a portion C in fig. 3.

In the figure: 1. a box body; 2. an upper cover; 3. a support plate; 4. a side cover; 5. a front cover; 6. a partition plate; 7. a first cross bar; 8. a second cross bar; 9. a first spring; 10. an arc-shaped plate; 11. a rubber pad; 12. a desiccant layer; 13. a circular through hole; 14. a limiting seat; 15. a reagent tube; 16. a box of the shape of the Chinese character hui; 17. a rectangular plate; 18. a second spring; 19. a U-shaped rod; 20. a U-shaped groove; 21. a sponge cushion; 22. a first magnet; 23. a second magnet; 24. a third magnet; 25. and a fourth magnet.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Examples

As shown in fig. 1 to 6, an embodiment of the present invention proposes: a kit for detecting nucleic acid of new coronavirus comprises a kit body 1, an upper cover 2 and a reagent tube 15, wherein the kit body 1 is connected with the upper cover 2 in a folding and rotating manner, a first rectangular groove is arranged in the kit body 1, the inner wall of the first rectangular groove is fixedly connected with a supporting plate 3, the supporting plate 3 is movably sleeved on the outer side of the reagent tube 15, the upper surface of the upper cover 2 is connected with a front cover 5 in a folding and rotating manner, the left side and the right side of the upper cover 2 are respectively connected with a side cover 4 in a folding and rotating manner, the upper surface of the supporting plate 3 is fixedly connected with a plurality of partition plates 6, the left side inner wall, the right side inner wall and the left side and the right side of each partition plate 6 are respectively and fixedly connected with a first cross rod 7, the outer side of each first cross rod 7 is slidably sleeved with a second cross rod 8, one end of each second cross rod 8 is respectively and fixedly connected with a first spring 9 between the corresponding partition plate 6 and the inner wall of the first rectangular groove, the first spring 9 is slidably sleeved on the outer side of the corresponding first cross rod 7, the equal fixedly connected with arc 10 of one end that is close to each other in the corresponding second horizontal pole 8 in a plurality of second horizontal poles 8, the bottom inner wall fixedly connected with drier layer 12 of first rectangular channel, a plurality of circular through-holes 13 have been seted up to the upper surface of drier layer 12, the spacing seat of last fixed surface of drier layer 12 14, spacing seat 14 and the outside of the reagent pipe 15 that corresponds, a plurality of shape boxes 16 that return of outside fixedly connected with of upper cover 2, the inner wall of the shape box 16 that returns is equipped with rectangular plate 17, fixedly connected with second spring 18 between the rear side of rectangular plate 17 and the rear side inner wall of the shape box 16 that returns that corresponds.

As shown in fig. 4, in some embodiments, a rubber pad 11 is fixedly connected to an inner wall of each of the plurality of arc-shaped plates 10, and the rubber pads 11 protect the tube body of the reagent tube 15 during the process of pressing the outer side of the reagent tube 15 by the arc-shaped plates 10.

As shown in fig. 2, in some embodiments, a U-shaped rod 19 is fixedly connected to the front side of the upper cover 2, a U-shaped groove 20 is formed in the upper surface of the box body 1, the outer side of the U-shaped rod 19 is movably contacted with the inner wall of the U-shaped groove 20, and the U-shaped rod 19 is engaged with the U-shaped groove 20, so that the sealing performance of the inner wall of the box body 1 is improved.

As shown in FIG. 2, in some embodiments, a sponge pad 21 is fixedly attached to the lower surface of the supporting plate 3.

As shown in fig. 1, in some embodiments, the front side of the front cover 5 and the front side of the box body 1 are both provided with a second rectangular groove, the inner walls of the two second rectangular grooves are fixedly connected with a first magnet 22 and a second magnet 23 respectively, the first magnet 22 and the second magnet 23 are magnetically attracted, and the upper cover 2 is conveniently fixed through the magnetic attraction of the first magnet 22 and the second magnet 23.

As shown in fig. 1, in some embodiments, third rectangular grooves are formed in the front sides of the two side covers 4 and the left and right sides of the box body 1, third magnets 24 are fixedly connected to the inner walls of the third rectangular grooves on the side covers 4, fourth magnets 25 are fixedly connected to the inner walls of the third rectangular grooves on the box body 1, the third magnets 24 and the corresponding fourth magnets 25 are magnetically adsorbed, and the upper cover 2 is conveniently fixed by the magnetic adsorption of the third magnets 24 and the corresponding fourth magnets 25.

As shown in fig. 6, in some embodiments, the outer sides of the rectangular plates 17 are slidably connected to the inner walls of the corresponding box 16.

In some embodiments, as shown in fig. 2, the supporting plate 3 and the sponge pad 21 are formed with a plurality of circular holes, and the inner walls of the circular holes are movably contacted with the outer sides of the reagent tubes 15.

The invention also provides a use method of the novel coronavirus nucleic acid detection kit, which comprises the following steps:

s1: a plurality of circular holes with the same size as the reagent tubes 15 are formed in the supporting plate 3 and the spongy cushion 21 through the hole digger;

s2: inserting the reagent tube 15 into the corresponding limiting seat 14 through the circular hole, and extruding the two corresponding arc-shaped plates 10 by the reagent tube 15 in the inserting process so that the two corresponding arc-shaped plates 10 move in the direction away from each other;

s3: the corresponding second cross rods 8 are driven to move in the process that the two corresponding arc-shaped plates 10 move in the direction away from each other, and the second cross rods 8 extrude the corresponding first springs 9 in the moving process;

s4: after the reagent tube 15 is inserted, the first spring 9 drives the corresponding arc-shaped plate 10 to fix the corresponding reagent tube 15 through the elasticity of the first spring, so that the reagent tube 15 cannot shake in the shaking and carrying process, and the purpose of improving the stability is achieved;

s5: after the reagent tube 15 is inserted, the upper cover 2 is rotated downwards, the upper cover 2 drives the shape returning box 16 to move downwards, the front side of the rectangular plate 17 is contacted with the upper end of the reagent tube 15 in the moving process, and the corresponding second spring 18 is extruded;

s6: when the upper cover 2 is completely covered, the first magnet 22 and the third magnet 24 on the front cover 5 and the side cover 4 are magnetically adsorbed with the corresponding second magnet 23 and the fourth magnet 25 on the box body 1, so that the upper cover 2 is fixed;

s7: when the upper cover 2 contacts with the upper surface of the box body 1, the U-shaped rod 19 is clamped with the inner wall of the U-shaped groove 20, so that the sealing property in the box body 1 is improved;

s8: in S5, the second spring 18 applies downward pressure to the reagent tube 15 by its own elastic force, so as to fasten the tube cover at the top end of the reagent tube 15, further enhancing the stability of the reagent tube 15;

s9: fully absorb the inside moisture of box 1 through drier layer 12, keep dry in the box 1, circular through-hole 13 makes and keeps ventilating in the box 1, provides a good air for reagent pipe 15.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.