1. A sample introduction system for introducing a sample into a microfluidic chip, the sample introduction system comprising: the device comprises a first suction and discharge device, a first rotary switching valve, a first storage pipeline, an air bin and a plurality of solution bins;

the first suction and discharge device is connected to a common valve port of the first rotary switching valve through the first storage pipe; independent valve ports of the first rotary switching valve are connected to a sample inlet of the microfluidic chip, the air bin and the plurality of solution bins in a one-to-one correspondence mode.

2. The sample injection system of claim 1, further comprising: a retarding sample injection unit;

in the independent valve ports of the first rotary switching valve, the independent valve port correspondingly connected to the sample inlet of the microfluidic chip is a first valve port; the first valve port is connected with a sample inlet of the microfluidic chip through the slow sample introduction unit;

the sample inlet sampling speed of the sample inlet is lower than the suction speed of the first suction and discharge device by the retarded sampling unit.

3. The sample injection system of claim 2, wherein the buffer unit comprises: the second suction and discharge device, the second rotary switching valve and the second storage pipeline;

the common valve port of the second rotary switching valve is connected with the sample inlet of the microfluidic chip through the second storage pipeline, and the independent valve ports of the second rotary switching valve are connected to the first valve port and the second suction and discharge device in a one-to-one correspondence manner.

4. The sample injection system of claim 3, wherein a suction and discharge velocity of the second suction and discharge device is less than a suction and discharge velocity of the first suction and discharge device.

5. The sample introduction system according to any one of claims 2 to 4, wherein the number of the buffer sample introduction units and the first valve ports is N, and N is the number of the sample introduction ports on the microfluidic chip;

each first valve port is connected with 1 corresponding sample inlet in the micro-fluidic chip through 1 corresponding sample inlet unit that retards.

6. The sample introduction system according to claim 3 or 4, wherein the first and/or second suction and discharge device comprises an assembly of any one or more of a syringe pump, a plunger pump, a membrane pump, a peristaltic pump.

7. The sample injection system of claim 1, wherein the first suction and discharge device, the first rotary switching valve, and the first storage conduit are all M in number, where M is the number of sample inlets on the microfluidic chip;

each first suction and discharge device is connected to a common valve port of the corresponding 1 first rotary switching valve through the corresponding 1 first storage pipeline.

8. The sample injection system of any of claims 1, 2, 3, 4, and 7, wherein the plurality of solution compartments comprises 1 sample compartment and at least 1 reagent compartment.

9. The sample introduction system of claim 8, wherein the at least 1 reagent cartridge comprises: wetting liquid storehouse, cleaner storehouse, waste liquid collection storehouse.

10. An instrument comprising a microfluidic chip and a sample introduction system according to any of claims 1 to 9.

Background

Microfluidics refers to the science and technology involved in systems that process or manipulate tiny fluids (nanoliters to microliters in volume) using microchannels (tens to hundreds of microns in size). Because of their miniaturization, integration, etc., microfluidic devices are commonly referred to as microfluidic chips, also known as lab-on-a-chip and micro total analysis systems.

At present, manual sample introduction is mostly adopted when the existing microfluidic chip performs sample introduction, that is, an operator manually places the same sample introduction tube into different sample bins or reagent bins in sequence so as to enable different samples or reagents to enter the microfluidic chip.

However, the manual sampling is adopted by an operator, and the efficiency of manually switching the same sampling tube to sample in different chambers is low, so that the efficiency of the instrument is low.

Disclosure of Invention

In view of this, embodiments of the present invention provide a sample injection system and an instrument, so as to solve the technical problem that an efficiency of manually switching a sample injection tube into different chambers is low due to manual sample injection by an operator, which results in a low efficiency of the instrument.

The embodiment of the invention provides a sample introduction system, which is used for introducing samples into a microfluidic chip and comprises: the device comprises a first suction and discharge device, a first rotary switching valve, a first storage pipeline, an air bin and a plurality of solution bins;

the first suction and discharge device is connected to a common valve port of the first rotary switching valve through a first storage pipeline; independent valve ports of the first rotary switching valve are connected to a sample inlet, an air bin and a plurality of solution bins of the microfluidic chip in a one-to-one correspondence mode.

So set up sampling system into autoinjection system, through set up first suction and discharge device and first rotatory diverter valve between solution storehouse and micro-fluidic chip, adopt first suction and discharge device and the cooperation of first rotatory diverter valve to advance the appearance, first suction and discharge device provides power for fluidic appearance of advancing, and first rotatory diverter valve can switch in each solution storehouse and advance the appearance, need not manual switching and advances the appearance, has improved appearance efficiency.

In some embodiments, which may include the above embodiments, further comprising: a retarding sample injection unit;

in the independent valve ports of the first rotary switching valve, the independent valve port correspondingly connected to the sample inlet of the microfluidic chip is a first valve port; the first valve port is connected with a sample inlet of the micro-fluidic chip through a retarding sample injection unit;

wherein, the sampling speed of the sample inlet by the retarding sampling unit is less than the suction speed of the first suction device.

In some embodiments that may include the above embodiments, the buffer sampling unit includes: the second suction and discharge device, the second rotary switching valve and the second storage pipeline;

the common valve port of the second rotary switching valve is connected with the sample inlet of the microfluidic chip through a second storage pipeline, and the independent valve ports of the second rotary switching valve are connected to the first valve port and the second suction and discharge device in a one-to-one correspondence manner.

In some embodiments, which may include the above embodiments, the suction and discharge speed of the second suction and discharge device is less than the suction and discharge speed of the first suction and discharge device.

In some embodiments, which may include the above embodiments, the number of the buffer sample injection units and the first valve ports is N, where N is the number of sample injection ports on the microfluidic chip;

each first valve port is connected with 1 corresponding sample inlet in the micro-fluidic chip through 1 corresponding sample inlet unit that slows down.

In some embodiments, which may include the above embodiments, the first and/or second suction and discharge devices comprise an assembly of any one or more of a syringe pump, a plunger pump, a diaphragm pump, a peristaltic pump.

In some embodiments, which may include the above embodiments, the number of the first suction and discharge devices, the first rotary switching valve, and the first storage conduits is M, where M is the number of the sample inlets on the microfluidic chip;

each first suction and discharge device is connected to the common valve port of the corresponding 1 first rotary switching valve through the corresponding 1 first storage pipeline.

In some embodiments, which may include the above embodiments, the plurality of solution compartments includes 1 sample compartment and at least 1 reagent compartment.

In some embodiments, which may include the above embodiments, the at least 1 reagent cartridge comprises: wetting liquid storehouse, cleaner storehouse, waste liquid collection storehouse.

In addition, the embodiment of the invention also provides an instrument which comprises the microfluidic chip and the sample injection system in the embodiment.

The sample introduction system and the sample introduction instrument provided by the embodiment comprise a first suction and discharge device, a first rotary switching valve, a first storage pipeline, an air bin and a plurality of solution bins; the first suction and discharge device is connected to a common valve port of the first rotary switching valve through a first storage pipeline; independent valve ports of the first rotary switching valve are connected to a sample inlet, an air bin and a plurality of solution bins of the microfluidic chip in a one-to-one correspondence mode. The first suction and discharge device is switched and communicated among the microfluidic chip, the air bin and the plurality of solution bins through the first rotary switching valve, so that the suction of fluids such as air, solution and the like is completed, and in addition, the fluids can be pushed into the microfluidic chip to complete sample introduction. Therefore, the sample introduction is not required to be manually switched, and the sample introduction efficiency is improved, so that the efficiency of the instrument is improved. The requirement on the precision of the equipment is low, so that the cost is reduced.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is easy to see that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.

Fig. 1 is a structural diagram of a first implementation manner of a sample injection system according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of a microfluidic chip according to an embodiment of the present invention;

fig. 3 is a structural diagram of a second implementation manner of a sample injection system according to an embodiment of the present invention.

Description of reference numerals:

10: a first suction and discharge device;

20: a first rotary switching valve;

21: a first valve port;

30: a first storage conduit;

41: an air bin;

42: a solution bin;

50: a microfluidic chip;

51: a sample inlet;

60: a retarding sample injection unit;

61: a second suction device;

62: a second rotary switching valve;

63: a second storage conduit.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Microfluidics refers to the science and technology involved in systems that process or manipulate tiny fluids (nanoliters to microliters in volume) using microchannels (tens to hundreds of microns in size). Because of their miniaturization, integration, etc., microfluidic devices are commonly referred to as microfluidic chips, also known as lab-on-a-chip and micro total analysis systems. One of the important features of microfluidics is the unique fluid properties in microscale environments, such as laminar flow and droplets. With these unique fluidic phenomena, microfluidics can achieve a range of microfabrication and micromanipulation that are difficult to accomplish with conventional methods. Microfluidics is considered to have great development potential and broad application prospects in biomedical research.

In the related art, when a microfluidic chip is used for sample separation and purification, an operator needs to connect the microfluidic chip with an external sample inlet tube in an aligned manner, and a sample, a reagent and other fluids flow into the microfluidic chip through the external sample inlet tube. The prior micro-fluidic chip adopts manual sample injection, that is, an operator manually puts the same sample injection tube into different solution bins in sequence, so that different samples or reagents enter the micro-fluidic chip.

However, the manual sampling is adopted by an operator, and the efficiency of manually switching the same sampling tube to sample in different solution bins is low, so that the efficiency of the instrument is low.

In order to solve the above problems, the present embodiment provides a sampling system and an apparatus, the sampling system is set as an automatic sampling system, a first suction and discharge device and a first rotary switching valve are disposed between a solution cabin and a microfluidic chip, the first suction and discharge device and the first rotary switching valve are used for matching sampling, the first suction and discharge device provides power for fluid sampling, the first rotary switching valve can switch sampling in each solution cabin, manual switching sampling is not needed, sampling efficiency is improved, and therefore efficiency of the apparatus is improved.

The instrument provided by this embodiment may be an instrument having a sample injection system, such as a blood analyzer, an immunoassay analyzer, a Circulating Tumor Cell (CTC) detector, and the like.

The instrument comprises a micro-fluidic chip and a sample introduction system, wherein the sample introduction system is communicated with the micro-fluidic chip. The sample introduction system provides samples for the microfluidic chip, the samples enter the microfluidic chip and then undergo experiments such as separation, purification, chemical reaction or culture, and the experimental results are obtained through direct observation or a detector in an instrument. Because the sample introduction system in the instrument is an automatic sample introduction system, the sample introduction is not required to be operated manually, and the efficiency of the instrument is improved.

The sample injection system provided by the embodiment is described in detail below with reference to the accompanying drawings.

Example one

In this embodiment, referring to fig. 1, the sample injection system includes a first suction and discharge device 10, a first rotary switching valve 20, a first storage pipeline 30, an air bin 41, and a plurality of solution bins 42. The air chamber 41 is used for accommodating air, and the solution chamber 42 is used for accommodating solutions such as samples and reagents. The first suction and discharge device 10 is used for sucking air, solution and the like from the air chamber 41 and the plurality of solution chambers 42, completing the air and solution sucking process, and the sucked solution partitioned by the air can be temporarily stored in the first storage pipe 30. The first suction and discharge device 10 may also discharge air, solution, and the like stored in the first storage pipe 30 into the microfluidic chip 50 by switching of the first rotary switching valve 20 to complete the sample injection of the microfluidic chip 50.

In order to complete the sampling of the air bin 41 and the plurality of solution bins 42 by the first suction and discharge device 10, a first rotary switching valve 20 may be provided between the first suction and discharge device 10 and the air bin 41 and the solution bins 42. The first suction and discharge device 10 switches sampling between the respective bin bodies by the first rotary switching valve 20.

The first rotary switch valve 20 is provided with a common valve port and a plurality of independent valve ports, wherein the independent valve ports are independent and not communicated with each other. Through the rotation switching, the common valve port can be communicated with the independent valve ports, and the common valve port is communicated with one of the independent valve ports at a time, so that the common valve port can be switched and communicated among different independent valve ports. Because the rotary switching valve is realized by rotary switching when the valve ports are switched, the impact on the liquid level of each valve port is reduced, and therefore, the error when the independent valve ports are switched can be effectively reduced by adopting the rotary switching valve in the embodiment.

The first suction and discharge device 10 is connected to a common port of the first rotary switching valve 20 through a first storage pipe 30; the independent valve ports of the first rotary switching valve 20 are connected to one sample inlet 51, the air chamber 41 and the plurality of solution chambers 42 of the microfluidic chip 50 in a one-to-one correspondence. The first suction and discharge device 10 is communicated to the air bin 41 and the plurality of solution bins 42 sequentially through the common valve port and the independent valve port of the first rotary switching valve 20, so that the first suction and discharge device 10 sucks air, solution and the like from the air bin 41 and the solution bins 42, the sucked solution is separated by air segments to form solution segments, and the solution segments can be temporarily stored in the first storage pipeline 30. In addition, the first suction and exhaust device 10 sequentially passes through the common valve port and the independent valve port of the first rotary switching valve 20 and is communicated to the sample inlet 51 of the microfluidic chip 50, so that the fluid temporarily stored in the first storage pipeline 30 can be pushed into the microfluidic chip 50, thereby realizing sample injection of the microfluidic chip 50. The connection manner of the first rotary switching valve 20, the air tank 41 and the plurality of solution tanks 42 may be direct interface connection, or may be connection through a pipeline, which is not limited herein.

Specifically, the independent valve ports of the first rotary switching valve 20 at least include: 1 independent valve port for communicating with the air chamber 41, 1 independent valve port for communicating with the microfluidic chip 50, and a plurality of independent valve ports for communicating with the solution chambers 42 in a one-to-one correspondence manner.

The sample introduction system provided by the present embodiment includes a first suction and discharge device 10, a first rotary switching valve 20, a first storage pipeline 30, an air bin 41, and a plurality of solution bins 42; the first suction and discharge device 10 is connected to a common port of the first rotary switching valve 20 through a first storage pipe 30; the independent valve ports of the first rotary switching valve 20 are connected to the sample inlet 51, the air chamber 41, and the plurality of solution chambers 42 of the microfluidic chip 50 in a one-to-one correspondence. The first suction and discharge device 10 is switched and communicated among the microfluidic chip 50, the air chamber 41 and the plurality of solution chambers 42 through the first rotary switching valve 20, so that the suction of fluids such as air and solution is completed, and in addition, the fluids can be pushed into the microfluidic chip 50 to complete the sample injection. Therefore, the sample introduction is not required to be manually switched, and the sample introduction efficiency is improved, so that the efficiency of the instrument is improved.

The common valve port of the first rotary switching valve 20 may be located in the middle, and each of the independent valve ports is located around the common valve port at intervals. Therefore, the pipeline between the independent valve port and the common valve port is short, the cleaning is easy, in addition, the flow path of the fluid such as air, solution and the like is short, and the sample introduction speed is high. Of course, the common valve port and the independent valve ports of the first rotary switching valve 20 may be distributed on the first rotary switching valve 20 in other manners, for example, the common valve port is located at one end of the first rotary switching valve 20, and each independent valve port is located at the other end of the first rotary switching valve 20.

Wherein the plurality of solution compartments 42 may include 1 sample compartment and at least 1 reagent compartment. The sample chamber may be used for accommodating a sample, and the at least one reagent chamber may include a wetting fluid chamber for accommodating a wetting fluid, a cleaning agent chamber for accommodating a cleaning agent, and a waste fluid collecting chamber for accommodating a waste fluid, including but not limited to the above-mentioned chamber bodies. It should be noted that the waste liquid collecting bin can be used for collecting the waste liquid, and first suction and discharge device 10 communicates the waste liquid collecting bin through first rotary switching valve 20 and can be used for discharging the inside waste liquid of sampling system into the waste liquid collecting bin, can not follow the waste liquid collecting bin and absorb the waste liquid.

Since a product may remain after the sample reacts in the microfluidic chip 50, the remaining product may be washed by the wetting fluid in the wetting fluid compartment to observe the reaction result.

The air in the air chamber 41 can isolate the wetting fluid between the fluids in the sample injection system from the sample, thereby avoiding the influence of the wetting fluid on the sample. Illustratively, the air reservoir 41 may be open to the atmosphere to take advantage of ambient air.

The cleaning agent in the cleaning agent bin can be used for cleaning the sample feeding system. Illustratively, the cleaning agent may be alcohol.

In this embodiment, the first suction and discharge device 10 may be any one or more of a syringe pump, a plunger pump, a diaphragm pump, and a peristaltic pump, as long as the first suction and discharge device 10 can provide power for the sample feeding system.

The present embodiment is described by taking a syringe pump as an example. The syringe pump may be in communication with a water tank, and the liquid in the water tank may be used to assist in propelling a flow of fluid such as air, solution, etc. in the sample injection system.

It is assumed that the solution compartment 42 includes a wetting solution compartment, a sample compartment, and a waste compartment. When the sampling system works, the first suction and discharge device 10 is sequentially communicated with the wetting fluid bin, the air bin 41 and the sample bin through the first rotary switching valve 20, and the first suction and discharge device 10 sequentially sucks the wetting fluid, the air and the sample into the first storage pipeline 30; then, the first suction and discharge device 10 is communicated with the sample inlet 51 of the microfluidic chip 50 through the first rotary switching valve 20, the wetting fluid, the air, the sample and the like in the first storage pipeline 30 are discharged into the microfluidic chip 50, the sample enters the microfluidic chip 50 to perform experiments such as reaction or culture, the experimental result is judged through direct observation or an auxiliary detector, finally, the pipeline is cleaned by using alcohol, and the waste liquid is discharged into a waste liquid bin. Thereby completing the experiment on the microfluidic chip 50.

In this embodiment, one first rotary switch valve 20 can perform a sample injection operation on one sample inlet 51 of the microfluidic chip 50, and the sample inlets 51 of the microfluidic chip 50 can be at least one, for example; 1, 2, 3, 4, 6, including but not limited to the above numbers. In the embodiment shown in fig. 1, in order to realize simultaneous injection to 4 injection ports 51, the number of the first suction and discharge device 10, the first rotary switching valve 20 and the first storage tube 30 may be equal to the number of the injection ports 51 of the microfluidic chip 50. Thus, one of the first suction and discharge devices 10, one of the first rotary switching valves 20, and one of the first storage tubes 30 correspond to one of the sample inlets 51 of the microfluidic chip 50.

Each of the first suction and discharge devices 10 is connected to the common port of a corresponding one of the first rotary switching valves 20 through a corresponding one of the first storage pipes 30. For example, the number of the sample inlets 51 of the microfluidic chip 50 is M, M first suction and discharge devices 10 suck M samples from the sample chamber through M first rotary switching valves 20 and enter M first storage pipelines 30, the suction principle of the wetting fluid, the air and the cleaning agent is similar, and M first suction and discharge devices 10 push M sucked fluids into M sample inlets 51 of the microfluidic chip 50 through M first rotary switching valves 20. So that the sample injection operation can be simultaneously performed on the M sample injection ports 51 of the microfluidic chip 50.

The samples taken from the sample bins can be the same sample or different samples. Referring to fig. 2, each sample inlet 51 of the microfluidic chip 50 corresponds to a corresponding detection item, and the detection items corresponding to the sample inlets 51 of the microfluidic chip 50 may be the same or different. Therefore, when the samples are the same sample, detection of different items of the same sample can be realized; or when the samples are different samples, the detection of the same item of different samples can be realized.

Example two

Since the solution entering the microfluidic chip 50 is only a few microliters, in order to improve the accuracy and stability of the sample injection system, the sample injection speed of the microfluidic chip 50 needs to be relatively slow. However, the first aspirating and discharging device with small volume and slow sample introduction is selected in the first embodiment, which often causes the speed of aspirating the sample to be too slow, resulting in a longer time for the whole sample introduction process. In order to achieve the purpose of rapidly sucking the samples, the reagents and the like from the various cabin bodies and simultaneously ensure that the samples are slowly pushed into the microfluidic chip 50, two processes of sucking the samples and pushing the samples into the microfluidic chip 50 are respectively processed by two suction and discharge devices, the samples are sucked by the suction and discharge devices with the high speed, and the samples are pushed into the microfluidic chip 50 by the sample feeding unit with the low speed.

The differences from the above embodiment are: in this embodiment, referring to fig. 3, the sample injection system may further include a buffer sample injection unit 60, and the first suction/discharge device 10 is communicated with the microfluidic chip 50 through the buffer sample injection unit 60. The sample feeding speed of the sample inlet 51 by the sample buffer unit 60 is less than the suction speed of the first suction device 10. The first suction and discharge device 10 sucks the sample, temporarily stores the sample in the sample slowing unit 60, and finally slowly discharges the sample into the microfluidic chip 50 through the sample slowing unit 60.

Of the independent valve ports of the first rotary switching valve 20, the independent valve port corresponding to the sample inlet 51 connected to the microfluidic chip 50 is the first valve port 21. The first valve port 21 corresponds to a sample outlet of the first rotary switch valve 20, and the fluid enters the microfluidic chip 50 through the first valve port 21. A buffer sample injection unit 60 is arranged between the first valve port 21 and the microfluidic chip 50, the first valve port 21 is connected with a sample injection port 51 of the microfluidic chip 50 through the buffer sample injection unit 60, that is, the fluid enters the sample injection port 51 of the microfluidic chip 50 after passing through the first valve port 21 of the first rotary switching valve 20 and the buffer sample injection unit 60 in sequence.

Specifically, as shown in fig. 3, the buffer sample injection unit 60 may include: a second suction and discharge device 61, a second rotary switching valve 62 and a second storage pipe 63. In order to realize the slow sample injection, the suction and discharge speed of the second suction and discharge device 61 may be less than that of the first suction and discharge device 10. The first suction and discharge device 10 pushes the fluid into the second storage tube 63 through the second rotary switching valve 62 for temporary storage, and the second suction and discharge device 61 pushes the fluid temporarily stored in the second storage tube 63 into the microfluidic chip 50 through the second rotary switching valve 62.

Wherein, the common port of the second rotary switching valve 62 is connected with the sample inlet 51 of the microfluidic chip 50 through the second storage tube 63, and the independent ports of the second rotary switching valve 62 are connected to the first ports 21 and the second suction/discharge device 61 in a one-to-one correspondence manner.

When the sampling system works, the first suction and discharge device 10 sequentially passes through the common valve port and the independent valve port of the first rotary switching valve 20, sucks the solution isolated by air from each bin body, and temporarily stores the sucked fluid in the first storage pipeline 30; then, the first suction and discharge device 10 pushes the fluid into the second storage pipe 63 sequentially through the common port of the first rotary switching valve 20, the first port 21, one independent port of the second rotary switching valve 62, and the common port of the second rotary switching valve 62; the second rotary switching valve 62 switches another independent valve port to communicate with the common valve port; the second suction and discharge device 61 pushes the fluid in the second storage pipeline 63 into the microfluidic chip 50 through another independent valve port and the common valve port switched by the second rotary switching valve 62 at a sample injection speed required by the chip, thereby realizing sample injection operation.

In this embodiment, one second rotary switching valve 62 can perform a sample buffer operation on one sample inlet 51 of the microfluidic chip 50, and there may be at least one sample inlet 51 of the microfluidic chip 50, and in order to realize simultaneous sample buffer for each sample inlet 51, the number of the buffer sample introduction units 60 and the number of the first valve ports 21 may be equal to the number of the sample inlets 51 on the microfluidic chip 50; each first valve port 21 is connected to a corresponding 1 sample inlet 51 of the microfluidic chip 50 through a corresponding 1 sample buffer unit 60. That is, the number of the second suction and discharge device 61, the second rotary switching valve 62, the first valve port 21 and the second storage tube 63 may be equal to the number of the sample inlets 51 of the microfluidic chip 50. One of the second suction and discharge devices 61, one of the second rotary switching valves 62, one of the second storage tubes 63, and one of the first valve ports 21 correspond to one of the sample inlets 51 of the microfluidic chip 50. Each first valve port 21 is connected to a corresponding one of the sample inlets 51 of the microfluidic chip 50 through a corresponding one of the second rotary switch valves 62.

For example, when the number of the sample inlets 51 of the microfluidic chip 50 is N, the first suction and discharge device 10 pushes N samples into N second storage channels 63 through N first valve ports 21 and N second rotary switching valves 62; then, the N second suction and discharge devices 61 push the N samples from the N second storage tubes 63 into the N sample inlets 51 of the microfluidic chip 50 through the N second rotary switching valves 62. So that the sample injection operation can be simultaneously performed on the N sample injection ports 51 of the microfluidic chip 50. In this case, the independent ports of the first rotary switching valve 20 include at least: n independent valve ports as the first valve port 21, one independent valve port connected to the air bin, and several independent valve ports connected to the solution bin in one-to-one correspondence.

In this embodiment, the second suction device 61 has a similar principle to the first suction device 10, and is not described again. For example, the second suction and discharge device 61 and the first suction and discharge device 10 may both be injection pumps, the first suction and discharge device 10 may use a 1mL syringe, and the suction and discharge speed of the first suction and discharge device 10 is relatively high; then, the second suction/discharge device 61 may use a 20 μ L or 50 μ L syringe, and the suction/discharge speed of the second suction/discharge device 61 is slower than that of the first suction/discharge device 10.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.