1. A preparation method of a fermentation waste liquid-based decarbonization cleaning agent comprises the following specific scheme:

step one, reaction sterilization, namely adding 2.4 to 3.8 parts of calcium chloride into 150 parts of fermentation waste liquid by mass, uniformly stirring and mixing, heating to 80 to 100 ℃, preserving heat for 5 to 20min, reacting to generate calcium proteinate, killing microorganisms contained in the fermentation waste liquid, and cooling to 20 to 40 ℃ after the reaction is finished;

step two, removing impurities by coagulation, namely adding 0.2-0.9 part of calcium hydroxide into fermentation liquor, adjusting the pH value to 7.5-10.5, adding 0.8-1.4 part of coagulant and 0.1-0.5 part of settling agent into fermentation waste liquor, stirring and coagulating for 10-30min, filtering, and discarding filter residues;

step three, carrying out oxidation treatment, namely stirring 10-16 parts by mass of 0.5-1.2mol/L of oxidant in the filtrate for reaction for 30-120min at room temperature;

step four, mixing, namely adding the fermentation waste liquid after oxidation treatment into a mixing kettle, adding 6.5-12.6 parts of sodium silicate, 3.6-8.2 parts of sodium hydroxide and 0.6-2.2 parts of sodium tripolyphosphate, controlling the temperature to be 35-55 ℃, stirring and mixing for 30-60min, and thus obtaining the fermentation waste liquid base decarbonization cleaning agent;

the method is characterized in that the fermentation waste liquid is cellulose cyst bacteria fermentation waste liquid.

2. The preparation method of the fermentation waste liquid-based carbon removal cleaning agent as claimed in claim 1, wherein the preparation method comprises the following steps: the settling agent is fly ash or diatomite or bentonite.

3. The preparation method of the fermentation waste liquid-based carbon removal cleaning agent as claimed in claim 1, wherein the preparation method comprises the following steps: the coagulant is polyaluminium sulfate, polyferric sulfate or polyaluminium ferric chloride.

4. The preparation method of the fermentation waste liquid-based carbon removal cleaning agent as claimed in claim 1, wherein the preparation method comprises the following steps: the oxidant is sodium peroxide or benzoyl peroxide or tert-butyl hydroperoxide.

5. The preparation method of the fermentation waste liquid-based carbon removal cleaning agent as claimed in claim 1, wherein the preparation method comprises the following steps: the cellulose cyst bacteria fermentation waste liquid is the fermentation waste liquid left after the epothilone is completely separated and extracted.

Background

After the automobile engine piston runs for a period of time, a layer of carbon deposit is formed on the top surface and the side surface of the piston, so that the phenomena of unstable idling of the engine, difficult starting of cold vehicles, frequent flameout, detonation, high-speed stall, power reduction, increased oil consumption and the like are caused.

CN102808191A discloses a biological carbon-removing cleaning agent, which comprises a biosurfactant, a high-efficiency surfactant, lipase, an auxiliary agent, a solvent, a spice and pure water. The biological carbon removal cleaning agent adopts the most advanced biotechnology at present, and integrates the dual functions of surface oil dirt cleaning degradation and softening carbon deposit shedding into a whole, thereby being a modern, environment-friendly, efficient and professional carbon removal cleaning agent. The biological carbon-removing cleaning agent has the advantages of effectively removing carbon deposition in an automobile combustion chamber and an air inlet manifold, reducing the oil consumption of an automobile, increasing power, prolonging the service life, improving the exhaust emission and the like with low energy consumption, low cost, high efficiency, high environmental protection and the like.

CN107686778A relates to a biological carbon removal cleaning agent, which is prepared from the following raw materials in parts by weight: 5-8 parts of polyisobutene amine, 6-9 parts of monoethanolamine, 3-5 parts of lipase, 4-8 parts of sodium alkyl benzene sulfonate, 1-3 parts of deodorized kerosene, 1-3 parts of spice and 30-50 parts of pure water. The invention has the advantages of environmental protection, high efficiency, effective removal of carbon deposition in the automobile combustion chamber and the intake manifold, reduction of automobile oil consumption, increase of power, prolongation of service life, improvement of tail gas emission and the like.

CN107236604A discloses a foam carbon deposition removal cleaning agent, which is prepared from 35-42% of nitrogen-methyl tetrahydropyrrolone, 2-5% of ethylene glycol monobutyl ether, 25-30% of deionized water, TERGTTOL 15-S-199-13%, 0.3-0.7% of ammonia water, 3-6% of morpholine, 0.1-0.4% of benzotriazole, 0.1-0.3% of sodium benzoate and 26-17% of propane butane LPG 2.713; the foam carbon deposition removing cleaning agent is suitable for various gasoline engines containing carburetors and injection systems, and can effectively decompose carbon deposition and compounds accumulated on valves, pistons, piston rings and intake manifolds, thereby improving the phenomena of fuel consumption, unstable idling and insufficient horsepower of automobiles, and simultaneously reducing the emission of carbon monoxide and incompletely combusted waste gas.

The cleaning agent for removing carbon deposition in the prior art and the invention contains a large amount of organic solvents and chemical agents, and the substances can cause secondary pollution to the environment while removing the carbon deposition, and even threaten the health of operators.

Disclosure of Invention

In order to solve the problems, the invention provides a decarbonization cleaning agent without secondary pollution, and the invention provides a preparation method of a fermentation waste liquid-based decarbonization cleaning agent.

A preparation method of a fermentation waste liquid-based decarbonization cleaning agent comprises the following specific scheme:

step one, reaction sterilization, namely adding 2.4 to 3.8 parts of calcium chloride into 150 parts of fermentation waste liquid by mass, uniformly stirring and mixing, heating to 80 to 100 ℃, preserving heat for 5 to 20min, reacting to generate calcium proteinate, killing microorganisms contained in the fermentation waste liquid, and cooling to 20 to 40 ℃ after the reaction is finished;

calcium chloride can promote the dissolution of protein, and Japanese scholars firstly dissolve cocoon filaments by calcium chloride or lithium bromide and the like in the study of silk crystalline structures, and later, WangJiannan and the like of Suzhou university in China have adopted the principle that calcium chloride can perform ion coordination reaction with side face hydroxyl of amino acid in protein in fermentation waste liquid to form chelate, so that secondary bonds among protein polypeptide chains in the fermentation waste liquid are destroyed, the bonding force among protein molecules is reduced, the structure is loose, and the calcium fibroin is dissolved in an aqueous solution.

Step two, removing impurities by coagulation, namely adding 0.2-0.9 part of calcium hydroxide into fermentation liquor, adjusting the pH value to 7.5-10.5, adding 0.8-1.4 part of coagulant and 0.1-0.5 part of settling agent into fermentation waste liquor, stirring and coagulating for 10-30min, filtering, and discarding filter residues;

step three, oxidation treatment, namely stirring 10-16 parts of oxidant with the molar weight of 0.5-1.2mol/L in the filtrate for reaction for 30-120min at room temperature;

the protein in the fermentation waste liquid can open disulfide bonds in the protein under the action of an oxidant, the folding and coiling structure of the protein is changed, and the disulfide bonds are oxidized into sulfonic groups, so that protein molecules have a good surface activity function, and the reaction formula is as follows:

step four, mixing, namely adding the fermentation waste liquid after oxidation treatment into a mixing kettle, adding 6.5-12.6 parts of sodium silicate, 3.6-8.2 parts of sodium hydroxide and 0.6-2.2 parts of sodium tripolyphosphate, controlling the temperature to be 35-55 ℃, stirring and mixing for 30-60min, and thus obtaining the fermentation waste liquid base decarbonization cleaning agent;

the method is characterized in that the fermentation waste liquid is cellulose cyst bacteria fermentation waste liquid.

The settling agent is fly ash or diatomite or bentonite.

The coagulant is polyaluminium sulfate, polyferric sulfate or polyaluminium ferric chloride.

The oxidant is sodium peroxide or benzoyl peroxide or tert-butyl hydroperoxide.

The cellulose cyst bacteria fermentation waste liquid is the fermentation waste liquid left after the epothilone is completely separated and extracted.

The invention provides a method for preparing a carbon-removing cleaning agent by using cellulomonas campestris fermentation waste liquid, and the carbon-removing cleaning agent prepared by the method does not contain any organic solvent or heavy metal substances, is safe and environment-friendly, does not contain organic volatile substances, and has the advantages of no harm to skin and no corrosion to workpieces.

The carbon removing cleaning agent can rapidly remove oil and carbon, and has high efficiency and quick response.

The carbon-removing cleaning agent disclosed by the invention fully utilizes the waste cellulosome cyst bacteria fermentation waste liquid, the treatment cost of the fermentation waste liquid is avoided, the waste is directly utilized, the waste is changed into the valuable, and the comprehensive cost of the carbon-removing cleaning agent is greatly reduced.

The carbon-removing cleaning agent disclosed by the invention is simple in preparation method, low in fixed investment, low in raw material cost, low in process energy consumption, and compounded with the development concepts of green chemistry and recycling economy.

Detailed Description

The invention is further illustrated by the following specific examples:

the cellulose cyst bacteria fermentation waste liquid adopted by the embodiment of the invention is the waste liquid generated by the culture liquid of inoculated GSUV3-205 type strains, and the fermentation culture medium comprises the following components in parts by weight: potato starch 0.25%, magnesium sulfate 0.12%, anhydrous calcium chloride 0.13%, glucose 0.22%, soybean powder 0.21%, skimmed milk powder 0.13%, vitamin B₁₂0.55mg/L ， EDTA-Fe³⁺1ml/L, 1ml/L of trace elements, 2.5 percent of adsorption resin (Amberlite XAD-16 resin (Rohm and Haas)), and the pH value of the fermentation liquor is pH 7.2.

Example 1

A preparation method of a fermentation waste liquid-based decarbonization cleaning agent comprises the following specific scheme:

adding 2.4kg of calcium chloride into 100kg of cellulosome cyst bacteria fermentation waste liquid, stirring and mixing uniformly, heating to 80 ℃, preserving heat for 5min, reacting to generate calcium proteinate, killing microorganisms contained in the fermentation waste liquid, and cooling to 20 ℃; then adding 0.2kg of calcium hydroxide into the fermentation liquor, adjusting the pH value to 7.5, adding 0.8kg of polyaluminium sulfate and 0.1kg of fly ash into the fermentation waste liquor, stirring and coagulating for 10min, filtering, and discarding filter residues; 10kg of sodium peroxide with the molar weight of 0.5mol/L is added into the filtrate, and the mixture is stirred and reacts for 30min at room temperature; and adding the oxidized fermentation waste liquid into a mixing kettle, adding 6.5kg of sodium silicate, 3.6kg of sodium hydroxide and 0.6kg of sodium tripolyphosphate, controlling the temperature to be 35 ℃, and stirring and mixing for 30min to obtain the fermentation waste liquid base decarbonization cleaning agent.

Example 2

A preparation method of a fermentation waste liquid-based decarbonization cleaning agent comprises the following specific scheme:

adding 2.8kg of calcium chloride into 130kg of cellulosome cyst bacteria fermentation waste liquid, stirring and mixing uniformly, heating to 90 ℃, preserving heat for 10min, reacting to generate calcium proteinate, killing microorganisms contained in the fermentation waste liquid, and cooling to 30 ℃ after completion; then adding 0.6kg of calcium hydroxide into the fermentation liquor, adjusting the pH value to 8.5, adding 1.2kg of polymeric ferric sulfate and 0.3kg of diatomite into the fermentation waste liquor, stirring and coagulating for 20min, filtering, and discarding filter residues; 13kg of benzoyl peroxide with the molar weight of 0.9mol/L is added into the filtrate, and the mixture is stirred and reacts for 60min at room temperature; and adding the oxidized fermentation waste liquid into a mixing kettle, adding 9.6kg of sodium silicate, 5.8kg of sodium hydroxide and 1.5kg of sodium tripolyphosphate, controlling the temperature to be 45 ℃, and stirring and mixing for 40min to obtain the fermentation waste liquid base decarbonization cleaning agent.

The settling agent is fly ash or diatomite or bentonite.

The coagulant is polyaluminium sulfate or polyferric sulfate polyaluminium ferric chloride.

The oxidant is sodium peroxide or benzoyl peroxide or sodium peroxide tert-butyl.

Example 3

A preparation method of a fermentation waste liquid-based decarbonization cleaning agent comprises the following specific scheme:

adding 3.8kg of calcium chloride into 150kg of cellulomonas cellulosae fermentation waste liquid, stirring and mixing uniformly, heating to 100 ℃, preserving heat for 20min, reacting to generate calcium proteinate, killing microorganisms contained in the fermentation waste liquid, and cooling to 40 ℃; then adding 0.9kg of calcium hydroxide into the fermentation liquor, adjusting the pH value to 10.5, adding 1.4kg of polyaluminum ferric chloride and 0.5kg of bentonite into the fermentation waste liquor, stirring and coagulating for 30min, filtering, and discarding filter residues; 16kg of tert-butyl hydroperoxide with the molar weight of 1.2mol/L is added into the filtrate, and the mixture is stirred and reacted for 120min at room temperature; and adding the oxidized fermentation waste liquid into a mixing kettle, adding 12.6kg of sodium silicate, 8.2kg of sodium hydroxide and 2.2kg of sodium tripolyphosphate, controlling the temperature to be 55 ℃, and stirring and mixing for 60min to obtain the fermentation waste liquid base decarbonization cleaning agent.

Comparative example 1

A preparation method of a fermentation waste liquid-based decarbonization cleaning agent comprises the following specific scheme:

adding 100kg of pure water and 4.8kg of soap into a mixing kettle, adding 6.5kg of sodium silicate, 3.6kg of sodium hydroxide and 0.6kg of sodium tripolyphosphate, controlling the temperature to be 35 ℃, and stirring and mixing for 30min to obtain the fermentation waste liquid base decarbonization cleaning agent.

Comparative example 2

A preparation method of a fermentation waste liquid-based decarbonization cleaning agent comprises the following specific scheme:

adding 2.4kg of calcium chloride into 100kg of cellulosome cyst bacteria fermentation waste liquid, stirring and mixing uniformly, heating to 80 ℃, preserving heat for 5min, reacting to generate calcium proteinate, killing microorganisms contained in the fermentation waste liquid, and cooling to 20 ℃; then adding 0.2kg of calcium hydroxide into the fermentation liquor, adjusting the pH value to 7.5, adding 0.8kg of polyaluminium sulfate and 0.1kg of fly ash into the fermentation waste liquor, stirring and coagulating for 10min, filtering, and discarding filter residues; 10kg of sodium peroxide with the molar weight of 0.5mol/L is added into the filtrate, and the mixture is stirred and reacts for 30min at room temperature; and adding the oxidized fermentation waste liquid into a mixing kettle, adding 3.6kg of sodium hydroxide and 0.6kg of sodium tripolyphosphate, controlling the temperature to be 35 ℃, stirring and mixing for 30min to obtain the fermentation waste liquid base decarbonization cleaning agent.

Comparative example 3

A preparation method of a fermentation waste liquid-based decarbonization cleaning agent comprises the following specific scheme:

adding 2.4kg of calcium chloride into 100kg of cellulosome cyst bacteria fermentation waste liquid, stirring and mixing uniformly, heating to 80 ℃, preserving heat for 5min, reacting to generate calcium proteinate, killing microorganisms contained in the fermentation waste liquid, and cooling to 20 ℃; then adding 0.2kg of calcium hydroxide into the fermentation liquor, adjusting the pH value to 7.5, adding 0.8kg of polyaluminium sulfate and 0.1kg of fly ash into the fermentation waste liquor, stirring and coagulating for 10min, filtering, and discarding filter residues; 10kg of sodium peroxide with the molar weight of 0.5mol/L is added into the filtrate, and the mixture is stirred and reacts for 30min at room temperature; and adding the oxidized fermentation waste liquid into a mixing kettle, adding 6.5kg of sodium silicate and 3.6kg of sodium hydroxide, controlling the temperature to be 35 ℃, stirring and mixing for 30min to obtain the fermentation waste liquid base decarbonization cleaning agent.

Comparative example 4

The branded gotterwei model was used: the decarbonizing cleaner of KB-8622 was used as a comparative sample.

The performance of the carbon removal cleaning agent prepared by the invention is measured. Foaming ability: adding the sample into a foaming machine by using the foaming machine, and pumping air to pressurize to 2KG/cm²The longer the foam is maintained, the better the bubble capacity of the sample. The carbon removal effect evaluation method comprises the following steps: and (3) putting the carbon steel sheet with the carbon deposit area of 10cm multiplied by 10cm into the sample, soaking for 10min, washing with clear water, and calculating the carbon removal efficiency according to the residual carbon deposit area.

The results of the performance test of the carbon removing agents of the above examples and comparative examples are shown in the following table:

	carbon removal efficiency (%)	Foaming time(s)
			Example 1	92.5	38
Example 2	94.1	42
			Example 3	94.9	45
Comparative example 1	74.1	21
			Comparative example 2	86.4	34
Comparative example 3	88.1	36
			Comparative example 4	90.2	38